• BMB Reports
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• v.33 no.5
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• pp.370-378
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• 2000

Incubation of purified laminin1-nidogen1 complexes with $[{\gamma}-^{32}P]-ATP$ in the presence of the catalytic subunit of the protein kinase A (cAMP-dependent protein kinase) resulted in the phosphorylation of the alpha chain of laminin-1 and of the nidogen-1 molecule. Aminoacid electrophoresis indicated that phosphate was incorporated on serine residues. The phosphorylation effect of laminin-1 on the process of self assembly was studied by turbidometry. In these experiments, the phosphorylated laminin-1 showed a reduced maximal aggregation capacity in comparison to the non-phosphorylated molecule. Examination of the laminin-1 network under the electron microscope showed that the phosphorylated sample formed mainly linear extended oligomers, in contrast to controls that formed large and dense multimeric aggregates. Heparin binding on phosphorylated laminin-1 in comparison to controls was also tested using solid-phase binding assays. The results indicated an enhanced heparin binding to the phosphorylated protein. The results of this study indicate that laminin1-nidogen1 is a substrate for protein kinase A in vitro. This phosphorylation had an obvious influence on the lamininl-nidogen1 network formation and the heparin binding capacity of this molecule. However, further studies are needed to investigate whether or not this phenomenon could play a role in the formation of the structure of basement membranes in vivo.

• Journal of Korean Society of Water and Wastewater
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• v.28 no.3
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• pp.325-337
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• 2014

The research on the actual condition of indoor asbestos concentration in Korea has not been thoroughly accomplished up to now. In this research the ratio of asbestos-containing buildings and indoor asbestos concentration was studied. This investigation was conducted in 2012 and 2013 and buildings were categorized based on region, building type by use, existing space(ground or basement), and construction year, respectively. Also the indoor asbestos concentration change was monitored to evaluation the efficiencies of two types of asbestos-concentration abatement devices. As a result, the ratio of asbestos-containing buildings in Seoul was largely decreased. The ratio of asbestos-containing buildings was higher in hospitals and schools regionally and in ground buildings than in basement. The average indoor asbestos concentrations were 0.0011, 0.0008 piece/cc in 2012 and 2013 investigation, respectively. Those values were much lower than standards(0.01 piece/cc), therefore the threat of indoor asbestos concentration might be negligible. In asbestos-concentration abatement experiments, the circulation velocity of ventilator were changed 2-6.7 m/sec. With 6.7 m/sec of velocity of ventilator, the concentration of indoor asbestos was fluctuated and maximum value was 2.4 piece/cc. With 4.5 and 2 m/sec of velocities of ventilator, the maximum concentration of indoor asbestos was fluctuated and maximum value was 0.9 piece/cc. This indicated that the concentration of indoor asbestos was decreased partly due to the free drop of asbestos. From these results, the proper velocity of ventilator seems to be between 4.7 and 6.5m/sec under this circumstance and further research is required. These research results may be used to guideline of asbestos management policy.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.11
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• pp.416-424
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• 2019

In Korea, press concrete in basements is mainly applied using plain concrete. This system has undesirable defects such as cracks caused by plastic shrinkage and irregular temperature distribution. To solve this problem, metal lath and fibers have been used in the past. However, they have not been effective in controlling cracks. This study analyzed the reduction of plastic shrinkage cracking for press concrete using various admixtures in a basement has been. In the air contents test, the specimens with various admixtures showed air contents similar to plain concrete (4.5±1.5%). The specimens using silica fume, super plasticizer agent, and SBR showed higher compressive strength by about 10-15% than plain concrete. Cracking decreased when the MC, super plasticizer, and SBR were added. When MC was used in the concrete, the plastic shrinkage did not occur.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.1
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• pp.814-821
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• 2015

The purpose of this study is to estimate crack reduction properties of covering concrete with fibers in basement. Air contents, slump, compressive strength, tensile strength and plastic shrinkage has been tested to conduct the optimum addition ratio and type of fiber. The results is a following. For the properties of air contents, all of the specimens added fibers shown the higher than plain concrete. For the flowability, slump decreased about 40-80% when all of the specimens added fibers. For the strength properties, the specimens added nylon fiber shown higher compressive and tensile strength about 5-15% comparing with other concrete. For the plastic shrinkage, cracking decreased when the fiber added comparing with plain concrete. Especially, when nylon fiber added in the concrete, the plastic shrinkage did not occurred. For the overall consideration, when the addition ratio of nylon fiber is 0.6%, the press concrete is identified as showed optimum properties.

• Childhood Kidney Diseases
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• v.13 no.2
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• pp.99-117
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• 2009

Diabetic nephropathy is a major cause of chronic renal failure in developing countries, and the prevalence rate has markedly increased during the past decade. Diabetic nephropathy shows various specific histological changes not only in the glomeruli but also in the tubulointerstitial region. In the early stage, the effacement of podocyte foot processes and thickened glomerular basement membrane (GBM) is noticed even at the stage of microalbuminuria. Nodular, diffuse, and exudative lesions, so-called diabetic glomerulosclerosis, are well known as glomerular lesions. Interstitial lesions also exhibit fibrosis, edema, and thickened tubular basement membrane. Diabetic nephropathy is considered to be multifactorial in origin with increasing evidence that one of the major pathways involved in the development and progression of diabetic nephropathy as a result of hyperglycemia. Hyperglycemia induces renal damage directly or through hemodynamic alterations, such as, glomerular hyperfiltration, shear stress, and microalbuminuria. Chronic hyperglycemia also induces nonhemodynamic dysregulations, such as, increased production of advanced glycosylation endproducts, oxidative stress, activation of signal pathway, and subsequent various cytokines. Those pathogenic mechanisms resulted in extracellular matrix deposition including mesangial expansion and GBM thickening, glomerular hypertrophy, inflammation, and proteinuria. In this review, recent opinions on the histopathologic changes and pathophysiologic mechanisms leading to initiation and progression of diabetic nephropathy will be introduced.

• Journal of the Korean Society of Groundwater Environment
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• v.3 no.2
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• pp.95-100
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• 1996

Schlumberger electrical soundings and Coincident loop time-domain electromagnetic soundings were made on the Nanji-do landfill to investigate the nature of fills and the subsurface structure. The measured data were transformed into apparent resistivity values and then inverted in terms of 1-D resistivity models. At 6 points, both measurements were carried out to check the validity of the interpreted subsurface electrical structures. Interpreted layered models from each method show a good agreement. Obtained models show that a conductive zone exist below the shallow resistive zone. Conductive zone, which is considered to be influenced by decomposition of organic waste materials and infiltration of precipitation, is terminated by resistive zone which is considered as basement. Considering the fact that conductive zone extends to the basement and there exist no barrier layers such as clay layers, contaminant plumes are likely to flow into the groundwater directly.

• Environmental Analysis Health and Toxicology
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• v.24 no.3
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• pp.203-211
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• 2009

A lesser degree of research is available with respect to indoor radon characteristics associated with occupants' exposure. The present study evaluated the radon levels in several public-access buildings or underground facilities, and their temporal variation in underground facilities. Radon measurements were conducted in 2005 and 2006, utilizing a continuous radon detector. A solid alpha detector (RAD7) was utilized to measure indoor radon levels. The mean radon concentrations obtained from the building or facilities were in a descending order: platforms of Daegu subway line 2, 2005 (32 $Bq/m^3$), hot-air bathroom (14 $Bq/m^3$), basement of office building (14 $Bq/m^3$), underground parking garage (14 $Bq/m^3$), underground shop (12 $Bq/m^3$), nursery (10 $Bq/m^3$), platforms of Daegu subway line 2, 2006 (9.0 $Bq/m^3$), platforms of Daegu subway line 1, 2006 (8.9 $Bq/m^3$), supermarket (7.9 $Bq/m^3$), hospital (7.3 $Bq/m^3$), and second-floor of office building (5.7 $Bq/m^3$). In general, underground-level facilities exhibited higher radon levels as compared with ground-level facilities. It was suggested that ventilation is an important parameter regarding the indoor levels of a subway. There was a decreasing or increasing trend in hourly-radon levels in a subway, whereas no trend were observed in a basement of office building. In addition, the radon levels in the subway lines 1 and 2 varied according to the platforms. The radon levels in the present study were much lower than those of previous studies. The average annual effective dose (AED) of radiation from indoor radon exposure was estimated to be between 0.043 and 0.242 mSv/yr, depending on facility types. These AEDs were substantially lower than the worldwide average AED (2.4 mSv/yr).

• Nutritional Sciences
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• v.7 no.3
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• pp.144-150
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• 2004

We have investigated the effect of calcium spirulan(Ca-SP) isolated from a blue-green alga Spirulina platensis, which is a sulfated polysaccharide chelating calcium and mainly composed of rhamnose and fructose, on invasion of both B16- BL6 melanoma cells, Colon 26 carcinoma and HT-1080 fibrosarcoma cells through reconstituted basement membrane (Matrigel). Ca-SP significantly inhibited the invasion of these tumor cells through Matrigel/fibronectin-coated filters in a concentration-dependent manner. Ca-SP also inhibited the haptotactic migration of tumor cells to laminin, but it had no inhibitory effect on tumor cell migration to fibronectin-coated filters. Ca-SP prevented the adhesion of B16-BL6 cells to Matrigel- and laminin-substrates but did not affect the adhesion to fibronectin. The pretreatment of tumor cells with Ca-SP inhibited the adhesion to laminin in a concentration-dependent fashion, while the pretreatment of laminin-substrates did not. Ca-SP had no effect on the production and activation of type IV collagenase in gelatin zymography. In contraset, Ca-SP significantly inhibited degradation of heparan sulfate by purified heparanase. The experimental lung metastasis was significantly reduced by co-injection of B16-BL6 cells with Ca-SP in a dose-dependent manner. Seven intermittent ⅰ.ⅴ. injection of 100$\mu\textrm{g}$ of Ca-SP caused a marked decrease of lung tumor colonization of B16-BL6 cells in a spontaneous lung metastasis model. These results suggest that Ca-SP, a novel sulfated polysaccharide, could reduce the lung colonization of B16-BL6 melanoma cells in experimental metastasis model, by inhibiting the tumor invasion of basement membrane Matrigel, probably through the prevention of the adhesion and migration of tumor cells to laminin-substrate and of the heparanase activity.

• Journal of the Korean Society for Railway
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• v.11 no.1
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• pp.95-100
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• 2008

The simulation analysis of fire-driven flow and passenger evacuation in Daegu subway station, Chung-Ang, have been performed. The first location of outbreak of fire is inside passenger car in the third basement in Chung-Aug station, The smoke flow in the second and third basement has been analyzed using FLUENT 6.2. The CO (carbon monoxide) and temperature distribution in the train units and station platform have been obtained and transferred to input data for evacuation simulation. The highest temperature in the train units was 1500k. For the simulation of passenger evacuation, EXODUS has been used for whole basements (level 1${\sim}$level 3) in the station. Total number of people was assumed to be one thousand and 640 were placed inside train and 360 were placed outside train. In evacuation simulation, an average of 135 passengers were killed and an average time to evacuate takes 10min 19sec. The main evacuation routes used by passengers were investigated and the cause of death was identified by evacuation simulation.

• Proceedings of the SCSK Conference
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• 2003.09a
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• pp.387-412
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• 2003

Influence of gelatinase on basement membrane (BM) structure was investigated by using a skin equivalent (SE) model. The results showed that (1) gelatinase produced by cells degraded the BM and (2) the addition of matrix metalloproteinase-specific inhibitor to the SE medium accelerated the formation of BM structure, indicating that gelatinase is involved in BM impairment. The activity of gelatinase was also studied in healthy human facial skin tissues. The result of in situ zymography revealed gelatinase activity around the basal layer of the epidermis, where BM integrity was severely compromised. Therefore, this enzyme was suggested to be associated with BM decomposition in human facial skin. To assess the behavior of gelatinase in stratum corneum (SC) non-invasively, an immunological study was performed. Since positive immunostaining of pro-gelatinase B was observed in SC stripped from sun-exposed skin, whereas no positive staining detected in SC of non-irradiated skin, gelatinase in the epidermis could be non-invasively detected by measuring gelatinase in SC. Gelatinase in SC of healthy female volunteers was monitored using a special film that sensitively and conveniently detects gelatinase. Ninetr percent of SC from facial skin (l00 women, 40's-50's) was gelatinase-positive. On the other hand, SC from non-irradiated skin was negative. These results strongly suggest that (1) gelatinase is constantly produced in the facial epidermis of most middle-aged woman during their daily life, and (2) the enzyme might be involved in the aging-related degeneration of both BM and the matrix fibers of the upper layer of the dermis, acting as a very important aging factor. Strong inhibitory activity against gelatinase was found in turmeric extract and identified curcumin as the major ingredient. Topical application of cream containing turmeric extract significantly decreased the number of gelatinase-positive SC clusters in human facial skins. These results indicated that turmeric is an effective ingredient to prevent skin from photo aging by suppressing chlonically upregulated gelatinase activity by UV and to improve skin condition.