• Title/Summary/Keyword: Base sequence

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The complete mitochondrial genome of the blue-tailed damselfly Ischnura elegans (Odonata: Coenagrionidae)-a climate-sensitive indicator species in South Korea

  • Seung Hyun Lee;Jeong Sun Park;Jee-Young Pyo;Sung-Soo Kim;Iksoo Kim
    • International Journal of Industrial Entomology and Biomaterials
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    • v.46 no.2
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    • pp.41-54
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    • 2023
  • The blue-tailed damselfly, Ischnura elegans Van der Linden, 1820 (Odonata: Coenagrionidae), is a climate-sensitive indicator species in South Korea. In this study, we sequenced the complete mitochondrial genome (mitogenome) of I. elegans collected from South Korea for subsequent population genetic analysis, particularly to trace population movements in response to climate change. The 15,963 base pair (bp)-long complete mitogenome of I. elegans has typical sets of genes including a major non-coding region (the A+T-rich region), and an arrangement identical to that observed in ancestral insect species. The ATP6, ND3 and ND1 genes have the TTG start codon, which, although rare, is the canonical start codon for animal mitochondrial tRNA. The A/T content was 71.4% in protein-coding genes, 72.1% in tRNAs, 72.9% in the whole genome, 74.7% in srRNA, 75.3% in lrRNA, and 83.8% in the A+T-rich region. The A+T-rich region is unusually long (1,196 bp) and contains two subunits (192 bp and 176-165 bp), each of which is tandemly triplicated and surrounded by non-repeat sequences. Comparison of the sequence divergence among available mitogenomes of I. elegans, including the one from the current study, revealed ND2 as the most variable gene, followed by COII and COI, suggesting that ND2 should be targeted first in subsequent population-level studies. Phylogenetic reconstruction based on all available mitogenome sequences of Coenagrionidae showed a strong sister relationship between I. elegans and I. senegalensis.

Method of Generating Information Signals in the System Industrial Internet of Things

  • Aleksandr Serkov;Nina Kuchuk;Bogdan Lazurenko;Alla Horiuskina
    • International Journal of Computer Science & Network Security
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    • v.24 no.4
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    • pp.206-210
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    • 2024
  • Industrial facilities that use modern IT technologies require the ensured reliability and security of information in automated enterprise management. Concurrently, so as to ensure a high quality of communication, it is necessary to expand the bandwidth of communication channels, which are limited by the physical parameters of the radio frequency spectrum. In order to overcome this contradiction, we propose the application of technology fundamental to ultra-wideband signals, in which the ratio between the bandwidth and its central part is greater than "one". For this reason, the information signal is emitted without a carrier frequency - simultaneously within the entire frequency band - provided that the signal level is lower than the noise level. For the transmission of information content, the method of positional-time coding is used, in which each information bit is encoded by hundreds of ultrashort pulses that arrive within a certain sequence. Mathematical models of signals and values observed in wireless communication systems with autocorrelation reception of modulated ultra-wideband signals are furthermore recommended. These assist in identifying features of the dependence of the error probability on the normalized signal-to-noise ratio and the signal base. Comparative analysis has shown that the best noise immunity of the systems considered in this paper is the communication system, which uses the time separation of the reference and information signals. During the first half of the bit interval, the switch closes the output of the transmitter directly to the generator of the ultra-wideband signal - forming a reference signal. In the middle of the bit interval, the switch alternates the output to one of two possible positions depending on the encoding signal - "zero" or "one", forming the information part of the ultra-wideband signal. It should also be noted that systems with autocorrelation reception and separate transmission of reference and information signals, provide a high level of structural signal secrecy. Furthermore, they provide the reliable transmission of digital information, especially in interference conditions.

First Record of the Hawaiian Ruby Cardinalfish, Apogon erythrinus (Apogonidae, Perciformes) in Korea (제주 연안에서 채집된 동갈돔과 한국 첫기록종, Apogon erythrinus)

  • Maeng Jin Kim;Song Hun Han;Choon Bok Song
    • Korean Journal of Ichthyology
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    • v.36 no.2
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    • pp.188-192
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    • 2024
  • This is the first report of Apogon erythrinus (Perciformes: Apogonidae) from Korea. A single specimen (33.6 mm SL) was collected by a fish pot from the coastal waters of Jeju-do Island on 28 October 2009. This species is characterized by having 5~6 predorsal scales, 7~9 developed gill rackers, end of second dorsal fin spine not reaching the middle of second dorsal fin base when depressed, and posterior margin of body scales reddish-brown. To confirm the correctness of species identification, the DNA cytochrome c oxidase subunit I sequence was obtained from the sample and compared with those of cardinalfish species recorded in the NCBI database. As a result, it was well-matched to A. erythrinus. We newly added this species to the Korean fish fauna and proposed a new Korean name, "Kueun-nun-eol-ge-bi-neul" because the eyes are large compared to its body.

Complete Genome Sequence of Massilia sp. KACC 81254BP Reveals a Potential for Degrading Polyhydroxyalkanoates

  • Sihyun An;Gyeongjun Cho;Jae-Hyung Ahn;Hang-Yeon Weon;Dayeon Kim;Young-Joon Ko;Jehyeong Yeon;Joon-hui Chung;Han Suk Choi;Jun Heo
    • Microbiology and Biotechnology Letters
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    • v.52 no.1
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    • pp.102-104
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    • 2024
  • Massilia sp. KACC 81254BP, isolated from a landfill on Jeju Island, Republic of Korea, possesses the capability to degrade polyhydroxyalkanoates (PHAs). The genomic analysis of strain KACC 81254BP consists of a circular chromosome comprising 5,028,452 base pairs with a DNA G+C content of 64.6%. This complete genome consists of a total of 4,513 genes, including those encoding the PHA degradation enzyme (PhaZ). This study offers valuable genomic insights into the enzymes responsible for degrading polyester plastics.

DNA Sequences Compression using Repeat technique and Selective Encryption using modified Huffman's Technique

  • Syed Mahamud Hossein; Debashis De; Pradeep Kumar Das Mohapatra
    • International Journal of Computer Science & Network Security
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    • v.24 no.8
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    • pp.85-104
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    • 2024
  • The DNA (Deoxyribonucleic Acid) database size increases tremendously transmuting from millions to billions in a year. Ergo for storing, probing the DNA database requires efficient lossless compression and encryption algorithm for secure communication. The DNA short pattern repetitions are of paramount characteristics in biological sequences. This algorithm is predicated on probing exact reiterate, substring substitute by corresponding ASCII code and engender a Library file, as a result get cumulating of the data stream. In this technique the data is secured utilizing ASCII value and engendering Library file which acts as a signature. The security of information is the most challenging question with veneration to the communication perspective. The selective encryption method is used for security purpose, this technique is applied on compressed data or in the library file or in both files. The fractional part of a message is encrypted in the selective encryption method keeping the remaining part unchanged, this is very paramount with reference to selective encryption system. The Huffman's algorithm is applied in the output of the first phase reiterate technique, including transmuting the Huffman's tree level position and node position for encryption. The mass demand is the minimum storage requirement and computation cost. Time and space complexity of Repeat algorithm are O(N2) and O(N). Time and space complexity of Huffman algorithm are O(n log n) and O(n log n). The artificial data of equipollent length is additionally tested by this algorithm. This modified Huffman technique reduces the compression rate & ratio. The experimental result shows that only 58% to 100% encryption on actual file is done when above 99% modification is in actual file can be observed and compression rate is 1.97bits/base.

Development of Information Extraction System from Multi Source Unstructured Documents for Knowledge Base Expansion (지식베이스 확장을 위한 멀티소스 비정형 문서에서의 정보 추출 시스템의 개발)

  • Choi, Hyunseung;Kim, Mintae;Kim, Wooju;Shin, Dongwook;Lee, Yong Hun
    • Journal of Intelligence and Information Systems
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    • v.24 no.4
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    • pp.111-136
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    • 2018
  • In this paper, we propose a methodology to extract answer information about queries from various types of unstructured documents collected from multi-sources existing on web in order to expand knowledge base. The proposed methodology is divided into the following steps. 1) Collect relevant documents from Wikipedia, Naver encyclopedia, and Naver news sources for "subject-predicate" separated queries and classify the proper documents. 2) Determine whether the sentence is suitable for extracting information and derive the confidence. 3) Based on the predicate feature, extract the information in the proper sentence and derive the overall confidence of the information extraction result. In order to evaluate the performance of the information extraction system, we selected 400 queries from the artificial intelligence speaker of SK-Telecom. Compared with the baseline model, it is confirmed that it shows higher performance index than the existing model. The contribution of this study is that we develop a sequence tagging model based on bi-directional LSTM-CRF using the predicate feature of the query, with this we developed a robust model that can maintain high recall performance even in various types of unstructured documents collected from multiple sources. The problem of information extraction for knowledge base extension should take into account heterogeneous characteristics of source-specific document types. The proposed methodology proved to extract information effectively from various types of unstructured documents compared to the baseline model. There is a limitation in previous research that the performance is poor when extracting information about the document type that is different from the training data. In addition, this study can prevent unnecessary information extraction attempts from the documents that do not include the answer information through the process for predicting the suitability of information extraction of documents and sentences before the information extraction step. It is meaningful that we provided a method that precision performance can be maintained even in actual web environment. The information extraction problem for the knowledge base expansion has the characteristic that it can not guarantee whether the document includes the correct answer because it is aimed at the unstructured document existing in the real web. When the question answering is performed on a real web, previous machine reading comprehension studies has a limitation that it shows a low level of precision because it frequently attempts to extract an answer even in a document in which there is no correct answer. The policy that predicts the suitability of document and sentence information extraction is meaningful in that it contributes to maintaining the performance of information extraction even in real web environment. The limitations of this study and future research directions are as follows. First, it is a problem related to data preprocessing. In this study, the unit of knowledge extraction is classified through the morphological analysis based on the open source Konlpy python package, and the information extraction result can be improperly performed because morphological analysis is not performed properly. To enhance the performance of information extraction results, it is necessary to develop an advanced morpheme analyzer. Second, it is a problem of entity ambiguity. The information extraction system of this study can not distinguish the same name that has different intention. If several people with the same name appear in the news, the system may not extract information about the intended query. In future research, it is necessary to take measures to identify the person with the same name. Third, it is a problem of evaluation query data. In this study, we selected 400 of user queries collected from SK Telecom 's interactive artificial intelligent speaker to evaluate the performance of the information extraction system. n this study, we developed evaluation data set using 800 documents (400 questions * 7 articles per question (1 Wikipedia, 3 Naver encyclopedia, 3 Naver news) by judging whether a correct answer is included or not. To ensure the external validity of the study, it is desirable to use more queries to determine the performance of the system. This is a costly activity that must be done manually. Future research needs to evaluate the system for more queries. It is also necessary to develop a Korean benchmark data set of information extraction system for queries from multi-source web documents to build an environment that can evaluate the results more objectively.

Development of Prevotella nigrescens ATCC $33563^T$-Specific PCR Primers (Prevotella nigrescens ATCC $33563^T$ 균주-특이 중합효소연쇄반응 프라이머 개발)

  • Song, Soo-Keun;Yoo, So-Young;Kim, Mi-Kwang;Kim, Hwa-Sook;Lim, Sun-A;Kim, Do-Kyung;Park, Jae-Yoon;Kook, Joong-Ki
    • Korean Journal of Microbiology
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    • v.44 no.3
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    • pp.212-220
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    • 2008
  • A Pn10 DNA probe was introduced as a Prevotella nigrescens ATCC $33563^T$-specific DNA probe. In that study, the specificity of the Pn10 was tested with only type or reference strains of 5 oral bacterial species. The purpose of this study is to evaluate the specificity of the Pn10 using the wild type strains of P. nigrescens and is to develop the P. nigrescens ATCC $33563^T$-specific PCR primers based on the nucleotide sequence of the Pn10. The specificity of the Pn10 DNA probe was determined by Southern blot analysis. The nucleotide sequence of Pn10 DNA probes was determined by chain termination method. The PCR primers were designed based on the nucleotide sequence of cloned DNA fragment. The data showed that Pn10 DNA probe were hybridized with the genomic DNAs from P. nigrescens ATCC $33563^T$ and KB6. The Pn10 homologous region, KB6-Pn10, of P. nigrescens KB6 was cloned by PCR and sequenced. The Pn10 and KB6-Pn10 DNA fragments were consisted of 1,875 bp and 1,873 bp, respectively. The percent identity of the two was 98.8% and the divergence of them was 0.6%. The two primer sets (Pn10-F-AC/ Pn10-R-AC and Pn10-F-A/ Pn10-R-A), designed base on the nucleotide sequences of Pn10 DNA probe, were specific to the P. nigrescens ATCC $33563^T$. The two PCR primer sets could detect as little as 4 pg of genomic DNA of P. nigrescens ATCC $33563^T$. These results indicate that the two PCR primer sets have proven useful for the identification of P. nigrescens ATCC $33563^T$, especially with regard to the maintenance of the strain.

Genetic Variation in Flammulina velutipes (팽이버섯의 유전적 변이)

  • Kim, Jong-Bong;Jeong, Ja-In
    • Journal of Life Science
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    • v.21 no.10
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    • pp.1434-1442
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    • 2011
  • A genetic variation within 29 strains of F. velutipes was analyzed by internal transcribed spacer (ITS) sequence analysis and random amplified polymorphic DNA (RAPD). Seven hundred and twenty base pairs were sequenced during the analysis of the ITS region, but no significant variation was observed among the 29 strains of F. velutipes. Sixteen out of 40 random primers amplified polymorphic RAPD fragment patterns. The polymorphic levels of RAPD bands by some primers (OPA-2,4,3,9,10,20) were very high in all 29 strains, with 3,030 fragments ranging between 200 and 2,000 bp. Intraspecific genetic dissimilarity of the 29 strains was calculated to range from 3.3% to 45% by Nei-Li's method using these 3,030 RAPD bands. The genetic variation among Korean strains was relatively high, with dissimilarities ranging between 17% and 38.6%. In the Neighbor-Joining analysis using the genetic dissimilarities based on RAPD, all 29 strains were classified into 5 clusters. Strains in each cluster showed specific characteristics according to their origin and strains. These results suggested that OPA and OPB primers could be used for developing molecular genetic markers and screening of unidentified (F. velutipes) strains.

Porcine LMNA Is a Positional Candidate Gene Associated with Growth and Fat Deposition

  • Choi, Bong-Hwan;Lee, Jung-Sim;Lee, Seung-Hwan;Kim, Seung-Chang;Kim, Sang-Wook;Kim, Kwan-Suk;Lee, Jun-Heon;Seong, Hwan-Hoo;Kim, Tae-Hun
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.12
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    • pp.1649-1659
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    • 2012
  • Crosses between Korean and Landrace pigs have revealed a large quantitative trait loci (QTL) region for fat deposition in a region (89 cM) of porcine chromosome 4 (SSC4). To more finely map this QTL region and identify candidate genes for this trait, comparative mapping of pig and human chromosomes was performed in the present study. A region in the human genome that corresponds to the porcine QTL region was identified in HSA1q21. Furthermore, the LMNA gene, which is tightly associated with fat augmentation in humans, was localized to this region. Radiation hybrid (RH) mapping using a Sus scrofa RH panel localized LMNA to a region of 90.3 cM in the porcine genome, distinct from microsatellite marker S0214 (87.3 cM). Two-point analysis showed that LMNA was linked to S0214, SW1996, and S0073 on SSC4 with logarithm (base 10) of odds scores of 20.98, 17.78, and 16.73, respectively. To clone the porcine LMNA gene and to delineate the genomic structure and sequences, including the 3'untranslated region (UTR), rapid amplification of cDNA ends was performed. The coding sequence of porcine LMNA consisted of 1,719 bp, flanked by a 5'UTR and a 3'UTR. Two synonymous single nucleotide polymorphisms (SNPs) were identified in exons 3 and 7. Association tests showed that the SNP located in exon 3 (A193A) was significantly associated with weight at 30 wks (p<0.01) and crude fat content (p<0.05). This association suggests that SNPs located in LMNA could be used for marker-assisted selection in pigs.

A Study on the Auction Schemes of Fish Commodities in the Koheung Region (고흥지역 수산물 경매시장의 특성에 관한 연구)

  • 강연실
    • The Journal of Fisheries Business Administration
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    • v.29 no.2
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    • pp.111-141
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    • 1998
  • Why are auctions so prevalent in fisheries fields\ulcorner One answer is, perhaps, that fisheries products have no standard value. The price of any catch of fish (at least of fish destined for the fresh fish market) depends on the demand and supply conditions at a specific moment of time, influenced by prospective market developments and prices must be remade for each transaction. There are various auction schemes including written-bid method in Korea. It is difficult to make decision the application of auction selling in market distribution. One reason is the absence of adequate data on which to base firm statements. There is very little precise information about the relative volume handled by auction scheme as compared with other sales schemes Because of the paucity reliable data, one must depend largely on qualitative in attempting to above this problem. This paper is designed to examine which auction scheme is more efficient as a economic tool by introducing the three auctions, as samples, utilized in Koheung peninsula. Koheung coast with abundant fisheries resource, locates on the Middle-South part in Korea and has three auction firm operating by fisheries cooperatives. fur selling of dry fish included sea weed, live fish and fresh fish respectively, As a result, 1 found that there are three interesting auction schemes as follow : 1. More than one winners are selected as buyers. The highest bidder can at first get fishes he wants to buy, second winner can get surplus fishes after the highest bidder employes one's privilege with the highest pay, and next winner would be a buyer if fishes would be left in sequence after being sold with higher price. Every fisherman can sell one' s fishes with equal unit price if he delivery it to the winner within one day. Therefore, all the vendors feel they are equal members of fisheries cooperation. 2. Written-bid pricing on the cover of handy book. It is easy to write and to erase the figure more than on the small black board, and is convenient also to keep in the pocket. 3. Auctioning on the fixed platform with fixed fish tank is a very fast auction scheme in spite of short displaying time. Auctioneer presides bidding at one place on the fixed platform, instead of moving, vendors should carry a container of live fishes in the fish tank into showing table in front of would-be buyers and auctioneer. Although the applicability of the auction system to a marketing problem depends in part on subjective considerations by those making decision, basically it is a matter of comparative economic efficiency. In general, if the scheme maximizes returns in relation to the effort expanded by both buyers and sellers, it will be utilized. If it does not, a more efficiency may take place over a period of time, but, even more important, those making decisions may become aware of the potentialities of new schemes. Therefore, in order to applicate the three interesting auction schemes introduced in this paper to other fisheries market, it is necessary not only to analyze many other auction schemes but also to compare the economic efficiency those schemes utilizing in other fisheries market.

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