• Journal of Microbiology and Biotechnology
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• 제22권6호
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• pp.742-753
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• 2012

Copper-containing compounds are introduced into the environment through agricultural chemicals, mining, and metal industries and cause severe detrimental effects on ecosystems. Certain microorganisms exposed to these stressors exhibit molecular mechanisms to maintain intracellular copper homeostasis and avoid toxicity. We have previously reported that the soil bacterial isolate Achromobacter sp. AO22 is multi-heavy metal tolerant and exhibits a mer operon associated with a Tn21 type transposon. The present study reports that AO22 also hosts a unique cop locus encoding copper homeostasis determinants. The putative cop genes were amplified from the strain AO22 using degenerate primers based on reported cop and pco sequences, and a constructed 10,552 base pair contig (GenBank Accession No. GU929214). BLAST analyses of the sequence revealed a unique cop locus of 10 complete open reading frames, designated copSRABGOFCDK, with unusual separation of copCD from copAB. The promoter areas exhibit two putative cop boxes, and copRS appear to be transcribed divergently from other genes. The putative protein CopA may be a copper oxidase involved in export to the periplasm, CopB is likely extracytoplasmic, CopC may be periplasmic, CopD is cytoplasmic/inner membrane, CopF is a P-type ATPase, and CopG, CopO, and CopK are likely copper chaperones. CopA, B, C, and D exhibit several potential copper ligands and CopS and CopR exhibit features of two-component regulatory systems. Sequences flanking indicate the AO22 cop locus may be present within a genomic island. Achromobacter sp. strain AO22 is thus an ideal candidate for understanding copper homeostasis mechanisms and exploiting them for copper biosensor or biosorption systems.

• Bulletin of the Korean Chemical Society
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• 제31권10호
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• pp.2813-2818
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• 2010

A novel method was developed for the speciation of chromium in natural water samples based on homogeneous liquid-liquid extraction and determination by flame atomic absorption spectrometry (FAAS). In this method, Cr(III) reacts with a new Schiff's base ligand to form the hydrophobic complex, which is subsequently entrapped in the sediment phase, whereas Cr(VI) remained in aqueous phase. The Cr(VI) assay is based on its reduction to Cr(III) by the addition of sodium sulfite to the sample solution. Thus, separation of Cr(III) and Cr(VI) could be realized. Homogeneous liquid-liquid extraction based on the pH-independent phase-separation process was investigated using a ternary solvent system (water-tetrabutylammonium ion ($TBA^+$)-chloroform) for the preconcentration of chromium. The phase separation phenomenon occurred by an ion-pair formation of TBA and perchlorate ion. Then sedimented phase was separated using a $100\;{\mu}L$ micro-syringe and diluted to 1.0 mL with ethanol. The sample was introduced into the flame by conventional aspiration. After the optimization of complexation and extraction conditions such as pH = 9.5, [ligand] = $1.0{\times}10^{-4}\;M$, [$TBA^+$] = $2.0{\times}10^{-2}\;M$, [$CHCl_3$] = $100.0\;{\mu}L$ and [$ClO_4$] = $2.0{\times}10{-2}\;M$, a preconcentration factor (Va/Vs) of 100 was obtained for only 10 mL of the sample. The relative standard deviation was 2.8% (n = 10). The limit of detection was sufficiently low and lie at ppb level. The proposed method was applied for the extraction and determination of chromium in natural water samples with satisfactory results.

• 한국식품영양과학회지
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• 제29권1호
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• pp.120-127
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• 2000

Deep-fat frying is a common cooking practice. There has been considerable concern regarding the mutagenic and carcinogenic potential of thermally oxidized oils. Studies on deep-fried foods so far have revealed not much on the mutagenicity of the oils in the foods. Therefore, in the present study, it was attempted to investigate the mutagenicity ofthe thermally oxidized safflower oil. Oil was heated in a home-fryer at a temperature of 180$\pm$3$^{\circ}C$ for 48 hours. Oil samples were taken at 0, 8, 16, 24, 32, 40 and 48 hours of heating, respectively. Each sample was used to study the changes in peroxide value (POV), acid value (AV), iodine value (IV), conjugated dienoic acid (CDA) content, %, and fatty acid composition. Another series of samples were fractionated into non-polar and polar fractions by column chromatography. The mutagenicity of the samples taken from the thermally oxidized oils, as well as the non-polar and polar fractions of the thermally oxidized oils, was investigated with the Ames test. The Ames test was carried out with and without metabolic activation. Bacterial tester strains used in the present study were the histidine auxotrophic strains of Salmonella typhimurium TA100, TA1535 and TA102 were used for the detection of base pair mutations, and TA98 and TA1537 for frame shift mutations. Each series of samples was dissolved in tetraphydrofuran (inhibitor-free) and tested at doses ranging from 0.05 to 5 mg/plate. None of the oil samples taken during the 48 hour oxidation period showed any mugagenic activity. This was the case, even after the activaton with 59 mix. Also, none of the polar and non-polar fractions showed any mutagenic activity on all the strains tested.

• 한국통신학회논문지
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• 제27권2A호
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• pp.137-145
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• 2002

3차원 모델링을 위한 방법 중 서로 다른 위치에서 획득한 두 영상으로부터 3차원 거리 정보를 추출하는 스테레오 영상 기법은 매우 유용한 방법이다. 일반적으로 2D 스테레오 영상으로부터 3차원 모델을 생성하기 위해서는 좌측 영상과 우측 영상의 일치하는 화소를 찾는 정합 과정에 의한 변위 추정이 매우 중요하다. 본 논문에서는 스테레오 영상의 변위 추정 문제를 해결하기 위해 기존 방법들의 장단점을 고려하여 변위의 정확성과 시간상의 효율성을 향상시킬 수 있는 방법을 제안한다. 빠른 수행을 위하여 먼저 스테레오 영상에 대하여 웨이블릿 변환을 수행하고 특징점 정합을 통하여 영상에서 기준이 될만한 기준점을 설정한다. 이 기준점은 95% 이상의 매칭 확률을 가지고 있는 점으로 이러한 기준점에 기초하여 영역 기반 방법의 조밀한 변위 추정을 위한 가변 블록 탐색창의 크기 결정과 오정합을 막기 위한 ordering constraint가 적용된다. 이렇게 하여 빠른 수행 시간안에 변위를 추정할 수 있었고, 영역 기반 방법의 문제점이었던 일정 크기의 윈도우를 일률적으로 적용하기 때문에 나타나는 폐색 영역의 문제나 반복 패턴으로 인한 오차를 해결할 수 있었다.

• Genomics & Informatics
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• 제3권2호
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• pp.61-65
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• 2005

Comparing 231 genes on chimpanzee chromosome 22 with their orthologous on human chromosome 21, we have found that 15 orthologs have indels within their coding sequences. It was rather surprising that significant number of genes have changed by indel, despite the shorter time since their divergence and led us hypothesize that indels and structural changes may represent one of the major mechanism of proteome evolution in the higher primates. Human T-complex protein 10 like (TCP 10L) is a representative having indel within its coding sequence. Gene structure of human TCP10L compared with chimpanzee TCP10L gene showed 16 base pair difference in genomic DNA. As a result of the indel, frame shift mutation occurs in coding sequence (CDS) and human TCP10L express longer polypeptide of 21 amino acid residues than that of chimpanzee. Our prediction found that the indel may affect to dramatic change of secondary protein structure between human and chimpanzee TCP10L. Especially, the structural changes in the C-terminal region of TCP10L protein may affect on the interacting potential to other proteins rather than DNA binding function of the protein. Through these changes, TCP10L might influence gene expression profiles in liver and testis and subsequently influence the physiological changes required in primate evolution.

• Animal cells and systems
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• 제3권1호
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• pp.89-96
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• 1999

Nucleotide sequences of a 501 base-pair (bp) fragment in the mitochondrial cytochrome b (cyt b) gene were analyzed for 12 populations of Rana rugosa from Korea and Japan using a polymerase chain reaction (PCR) and direct silver sequencing. Two genetically distinct groups (type-A and type-B) were found in Korea. Type-A was found throughout most of South Korea and type-B was restricted to the mid-southeastern regions (Samchok, Yongdok, Chongsong and Pohang). But in the Tonghae population, both types were found. The level of mitochondrial DNA (mtDNA) sequence differences ranged from 0% to .0.8% among six populations of type-A, and 0 to 1.0% among 4 populations of type-B. However, sequence differences between type-A and type-B ranged from 5.4% to 6.6%, Using Kimura's two-parameter distance, the level of genetic sequence divergence between type-A and type-B was 6.7%. The Japanese R. rugosa was clustered very far from the Korean R. rugosa with 14.7%. In the neighbor-joining and UPGMA tree, all Korean samples were grouped, but subdivided into two types in 99% of the bootstrap iteration.

• 한국정보통신학회논문지
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• 제18권11호
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• pp.2613-2620
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• 2014

본 논문에서는 전이중(full duplexing) 모드로 동작하는 기지국과 시분할 이중(TDD: Time Division Duplexing) 모 드로 동작하는 사용자들이 통신하는 전이중 무선 셀룰라 네트워크를 고려한다. 전이중 모드 사용시 상향링크 사용자 는 하향링크 사용자에게 간섭을 유발하므로 시스템 성능을 극대화하기 위해서는 같은 무선 자원을 동시에 사용하는 상 하향링크 사용자 쌍을 어떻게 결정하는 지가 매우 중요하다. 본 논문은 셀 용량을 최대화하기 위한 최적화 문제 를 제시하고 낮은 복잡도를 갖는 차선의 사용자 스케쥴링 알고리즘을 제안한다. 제안하는 스케쥴링 방식은 더 좋은 신호 품질을 갖는 하향링크 사용자가 자신에게 간섭을 덜 미치게 하는 상향링크 사용자를 먼저 선택할 수 있도록 우 선권을 갖는 방식으로 동작한다. 시뮬레이션 결과 제안 사용자 스케쥴링 방식을 사용하는 전이중 시스템은 최적 성 능을 달성하면서 기존 TDD 시스템의 셀 용량을 현저히 개선한다.

• 정보과학회 논문지
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• 제43권1호
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• pp.40-46
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• 2016

도로지도 생성은 인공위성 촬영이나 현장실사를 기반으로 한다. 그리하여 도로지도를 생성하고 수정하는데 많은 시간과 비용이 든다. 이러한 이유로 차량 GPS 데이터를 이용해 도로지도를 생성하는 연구가 활발히 진행되고 있다. 도로지도 생성 연구에서 가장 중요한 문제는 주도로와 같은 대표궤적을 추출하는 것이다. 대표궤적 추출을 수행할 때에는 시작과 끝이 비슷한 궤적데이터들의 집합을 전제로 하여 궤적을 추출한다. 따라서 대표궤적을 추출하기에 앞서 전처리 과정으로 궤적 클러스터링 작업이 필요하다. 본 논문에서는 이러한 문제를 해결하기 위해 하나의 영역을 일정한 격자로 분할하고, Sweep Line 알고리즘을 응용해 유사궤적들을 탐색한다. 마지막으로 프레쉐거리를 이용하여 궤적 간 유사도를 계산하였다. 실제로 서울의 강남구 지역에 있는 500대의 차량 GPS 궤적을 가지고 클러스터링 작업을 수행하였다. 또한, 실험을 통하여 격자분할 접근방식의 빠른 수행시간과 안정성을 보였다.

• 한국동물위생학회지
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• 제28권4호
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• pp.375-385
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• 2005

The present study was conducted to investigate the genetic profile of two prevalent avian pathogens in Korea namely, Newcastle disease virus (NDV) and infectious bursal disease virus (IBDV). Two farms located in Yeongi-gun, Chungnam were selected for this study. The two viruses were isolated from various organs (spleen, trachea, bursa of Fabricius) of deceased chickens that showed clinical symptoms of Newcastle Disease or Infectious bursal disease like swelling and congestion of the F bursa, facial edema, lacrimation, greenish yellow diarrhea as well as pathological signs like airsacculitis, haemorrhages in the intestines and so on. For analysis of NDV and IBDV, a 466 and 435 base pair fragments corresponding to the HN and VP2 regions which are highly conserved among related strains of NDV and IBDV, respectively, were amplified by RT-PCR and analyzed by sequencing. Comparison of the VP2 region showed a $99.3\%$ homology between the Korean IBDV isolate and the BJ836-attenuated vaccine strain. In contrast, the HN region of the Korean NDV isolate only has an 83 to $84\%$ homology with the vaccine strains LaSota, B1 and VGGA. Our findings reveal that the prevalent NDV strain in Korea is genetically different from the vaccine strains and may explain the recent outbreaks of Newcastle disease in the region.

• Parasites, Hosts and Diseases
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• 제53권5호
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• pp.611-618
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• 2015

This study describes the first record of Globocephalus samoensis (Nematoda: Ancylostomatidae) recovered in wild boars from southwestern regions of Korea. Gastrointestinal tracts of 111 Korean wild boars (Sus scrofa coreanus) hunted from mountains in Suncheon-si, Gwangyang-si, and Boseong-gun between 2009 and 2012 were examined for their visceral helminths. G. samoensis, as identified by morphological characteristics of the head and tail, were recovered from the small intestine of 51 (45.9%) wild boars. Worms were found from 7 of 28 wild boars (25.0%) from Suncheon-si, 40 of 79 (50.6%) from Gwangyang-si, and all 4 (100%) from Boseong-gun. The length of adult females was $7.2{\pm}0.5mm$, and the thickest part of the body measured the average $0.47{\pm}0.03mm$, while those of males were $6.52{\pm}0.19$ and $0.37{\pm}0.02mm$, respectively. The buccal cavity was equipped with a pair of large and bicuspid subventral lancets near the base of the capsule. The average length of spicules of males was $0.45{\pm}0.02mm$. By the present study, G. samoensis is recorded for the first time in southwestern regions of Korea. Additionally, morphological characteristics and identification keys provided in the present study will be helpful in the faunistic and taxonomic studies for strongylid nematodes in both domestic and wild pigs. The infection of G. samoensis apparently did not elicit pathologic lesions, as revealed by macroscopic observation during the autopsy of all wild boars in this study.