• Bulletin of the Korean Chemical Society
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• 제28권3호
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• pp.386-390
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• 2007

By use of a simple two-point extrapolation scheme estimating the correlation energies of the molecules along with the basis sets specifically targeted for extrapolation, we have shown that the MP2 basis set limit binding energies of large hydrogen-bonded complexes can be accurately predicted with relatively small amount of computational cost. The basis sets employed for computation and extrapolation consist of the smallest correlation consistent basis set cc-pVDZ and another basis set made of the cc-pVDZ set plus highest angular momentum polarization functions from the cc-pVTZ set, both of which were then augmented by diffuse functions centered on the heavy atoms except hydrogen in the complex. The correlation energy extrapolation formula takes the (X+1)-3 form with X corresponding to 2.0 for the cc-pVDZ set and 2.3 for the other basis set. The estimated MP2 basis set limit binding energies for water hexamer, hydrogen fluoride pentamer, alaninewater, phenol-water, and guanine-cytosine base pair complexes of nucleic acid by this method are 45.2(45.9), 36.1(37.5), 10.9(10.7), 7.1(6.9), and 27.6(27.7) kcal/mol, respectively, with the values in parentheses representing the reference basis set limit values. A comparison with the DFT results by B3LYP method clearly manifests the effectiveness and accuracy of this method in the study of large hydrogen-bonded complexes.

• 한국임상수의학회지
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• 제29권4호
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• pp.306-308
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• 2012

1세의 암컷 애완용 토끼(라이온 헤드, 체중:1.1kg)가 바이러스성 출혈병에 대한 예방접종을 하기 위해 병원에 내원하였다. 평소 야생고양이와 자주 접촉한 사실을 문진상에서 확인하여 분변을 채취하여 PCR검사를 실시한 결과 톡소플라즈마 B1 유전자를 검출하였기에 이를 보고하고자 한다.

• Journal of Microbiology and Biotechnology
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• 제5권4호
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• pp.181-187
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• 1995

The cells of Vibrio vulnificus can be induced to the viable but nonculturable (VBNC) state by natural environmental parameters. The V. vulnificus cells in the VBNC state can not be recovered by ordinary laboratory techniques. This nonculturability could often hamper development of effective processing strategies to minimize the number of V. vulnificus in seafoods. Even with V. vulnificus cells in a culturable state, the length of time required to identify the bacteria in contaminated food by phenotyphic characterization may prevent appropriate in-time responses by public health agencies to infections of the bacteria. In the present study, we used polymerase chain reaction (PCR) to develop a rapid and direct detection method for V. vulnificus in small octopus (Octopus variabilis) which is consumed as a raw food in Korea. The region targeted was a 704-base pair (bp) portion of the hemolysin gene, vvhA, of V. vulnificus. The primers designed for PCR amplification were specific for all V. vulnificus sp. tested. Several methods were examined to extract total DNA directly from V. vulnificus seeded into the octopus homogenate and the guanidine isothiocyanate (CITC) method appeared to be most effective. From the octopus homogenate seeded by V. vulnificus at an initial level of $10^2$ CFU/ml of the homogenate and then incubated for 12 h, the targeted sequence was successfully amplified by PCR and the 704-bp DNA fragment was observed by gel electrophoresis. The total completion of this assay requires less than one day.

• 한국CDE학회논문집
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• 제9권1호
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• pp.51-61
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• 2004

Mechanical parts are often grouped into part families based on the similarity of their shapes, to support efficient manufacturing process planning and design modification. The 2-part sequence papers present similarity assessment techniques to support part family classification for machined parts. These exploit the multiple feature decompositions obtained by the feature recognition method using convex decomposition. Convex decomposition provides a hierarchical volumetric representation of a part, organized in an outside-in hierarchy. It provides local accessibility directions, which supports abstract and qualitative similarity assessment. It is converted to a Form Feature Decomposition (FFD), which represents a part using form features intrinsic to the shape of the part. This supports abstract and qualitative similarity assessment using positive feature volumes.. FFD is converted to Negative Feature Decomposition (NFD), which represents a part as a base component and negative machining features. This supports a detailed, quantitative similarity assessment technique that measures the similarity between machined parts and associated machining processes implied by two parts' NFDs. Features of the NFD are organized into branch groups to capture the NFD hierarchy and feature interrelations. Branch groups of two parts' NFDs are matched to obtain pairs, and then features within each pair of branch groups are compared, exploiting feature type, size, machining direction, and other information relevant to machining processes. This paper, the second one of the two companion papers, describes the similarity assessment method using NFD.

• 한국CDE학회논문집
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• 제9권1호
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• pp.44-50
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• 2004

Mechanical parts are often grouped into part families based on the similarity of their shapes, to support efficient manufacturing process planning and design modification. The 2-part sequence papers present similarity assessment techniques to support part family classification for machined parts. These exploit the multiple feature decompositions obtained by the feature recognition method using convex decomposition. Convex decomposition provides a hierarchical volumetric representation of a part, organized in an outside-in hierarchy. It provides local accessibility directions, which supports abstract and qualitative similarity assessment. It is converted to a Form Feature Decomposition (FFD), which represents a part using form features intrinsic to the shape of the part. This supports abstract and qualitative similarity assessment using positive feature volumes. FFD is converted to Negative Feature Decomposition (NFD), which represents a part as a base component and negative machining features. This supports a detailed, quantitative similarity assessment technique that measures the similarity between machined parts and associated machining processes implied by two parts' NFDs. Features of the NFD are organized into branch groups to capture the NFD hierarchy and feature interrelations. Branch groups of two parts' NFDs are matched to obtain pairs, and then features within each pair of branch groups are compared, exploiting feature type, size, machining direction, and other information relevant to machining processes. This paper, the first one of the two companion papers, describes the similarity assessment methods using convex decomposition and FFD.

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2005년도 추계학술발표대회 및 정기총회
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• pp.15-18
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• 2005

A ribonucleic acid (RNA) is one of the two types of nucleic acids found in living organisms. An RNA molecule represents a long chain of monomers called nucleotides. The sequence of nucleotides of an RNA molecule constitutes its primary structure, and the pattern of pairing between nucleotides determines the secondary structure of an RNA. Non-coding RNA genes produce transcripts that exert their function without ever producing proteins. Predicting the secondary structure of non-coding RNAs is very important for understanding their functions. We focus on Nussinov's algorithm as useful techniques for predicting RNA secondary structures. We introduce a new traceback matrix and scoring table to improve above algorithm. And the improved prediction algorithm provides better levels of performance than the originals.

• 한국수산과학회지
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• 제52권6호
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• pp.696-701
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• 2019

Five specimens (839 - 1280 mm TL, Total length) of the dusky shark Carcharhinus obscurus, belonging to the family Carcharhinidae, were collected from the waters off Jejudo Island, Sinan, Namhae, Busan and Gangneung, Korea, during 2010-2019. Carcharhinus obscurus was similar to Carcharhinus brachyurus but was distinguished by the interdorsal ridge (present in C. obscurus vs. absent in C. brachyurus) and the shape of upper jaw teeth (broad in C. obscurus vs. narrow in C. brachyurus). In addition, 479 base-pair sequences in the mitochondrial DNA cytochrome c oxidase subunit I (COI) of our two specimens corresponded to those of C. obscurus (genetic distance, 0.000-0.003), but clearly distinguished from those of the Korean C. brachyurus (genetic distance, 0.03). We adopted the Korean name "Heuk-sang-eo" for Carcharhinus obscurus, after Kim and Ryu (2017).

• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1997년도 추계학술대회
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• pp.475-477
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• 1997

We have analyzed the fluorescence image obtaining from green fluorescence protein (GFP). In order to monitor the fluorescence of specific gene, we used the amyloid precursor protein promoter which has been known to act as a major role in the development of Alzheimer's disease. The promoter from - 3.0 kb to + 100 base pair was inserted into the gene expression monitoring GFP vector purchased from Clontech. This construct was transfected into the PC 12 and fibroblast cells and the fluorescence image was captured by two kinds of methods. One is using cheaper CCD camera and other is SIT-CCD camera. or the higher sensitivity of the fluorescence image, we developed the multiple image grabbing program. As a results, the fluorescence image by conventional CCD camera have the similar sensitivity compared with that of the SIT-camera by applying the multiple image grabbing programs. By this system. it will be possible to construct the fluorescence monitoring system with lower cost. And gene expression in real time by fluorescence image will be possible without changing the fluorescence images.

• 품질경영학회지
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• 제45권4호
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• pp.829-846
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• 2017

Purpose: This paper compares the effectiveness of typical selective assembly criteria and suggests the most promising one. Based on the result of a computer simulation, the key issues of selective assembly are examined and the best criterion is recommended from the effectiveness perspective. Methods: Using JAVA program, production of ten thousand units for each pair of components are simulated for selective assembly of the two types. And the number of mismatching and the fraction nonconforming for each criterion are determined. Results: The best match criterion appeared to be most promising from the perspectives of both mismatching and nonconforming problems. Its effectiveness appears to be also good even when the precision of one component is different from that of the other. Conclusion: For designing an optimal method for selective assembly, the best match criterion is recommendable as the base criterion.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2003년도 International Symposium
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• pp.89-110
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• 2003

최근 현장(In situ)에서의 토양 및 지하수 오염 정화는 오염물질을 현장에서 추출해 지상에서 처리하는 것이 아니라, 직접 현장에서 미생물 혹은 화학물질과 오염물질의 혼합을 통한 분해기 작을 이용해, 오염물질을 제거하는 과정을 의미한다. 이러한 현장오염정화 기법 중 하나가 Figure 1에 도시한 수직흐름처리정(Horizontal Flow Treatment Wells, HFTWs)으로 2개의 정(well) 중간지점에 반응물질을 설치한 후 각각의 정에서 상향 및 하향흐름을 발생시켜 주위의 지하수를 순환시키면서 정화하는 기법이다.