• Title/Summary/Keyword: Base pair

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The 18s rDNA Sequences of the Basidiocarps of Tricholoma matsutake in Korea (한국산 송이버섯에서의 18s ribosomal DNA 서열)

  • Lee, Sang-Sun;Hong, Sung-Woon
    • The Korean Journal of Mycology
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    • v.26 no.2 s.85
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    • pp.256-264
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    • 1998
  • The 18S rDNA sequences of Tricholoma matsutake (TM=T. caligatum var. nauseoum) collected in Korea were analyzed for the ectomycorrhizal fungi in the roots of Pinus densiflora. The 514 base pairs of rDNA region were synthesized by UF-5 and UR-6 primers, and double checked in the base pair. The sequence of four strains synthesized were all identical in this work, but different from those done by the previous workers. The basidiocarps collected in this work. were identified to T. matstake after searching the 18s rDNA by the BLAST in NCBI. Only several base pairs of 18S rDNA analyzed from other related basidiocarps were different from our analyses of 18S rDNA. The dendrogram were made based on the sequences of the 514 bp 18S rDNA by CLUSTAL-X alignment program. The groupings of the species at the level of genus in the dendrogram were well constructed.

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Molecular Identification and Morphological Development of Larvae of Psettina tosana Collected from Southern Sea of Korea (한국 남해에서 채집된 사량넙치 Psettina tosana 자어의 분자 동정 및 형태 발달)

  • Ji, Jae-Min;Yu, Hyo-Jae;Hwang, Kang-Seok;Park, Jeong-Ho;Lee, Jeong-Hoon;Kim, Jin-Koo
    • Korean Journal of Ichthyology
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    • v.29 no.4
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    • pp.244-251
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    • 2017
  • A total of 15 larvae [3.53~19.49 mm standard length (SL)] belonging to the family Bothidae collected from the southern sea of Korea in 2016 were identified as Psettina tosana based on 434 base-pair sequences of mitochondrial DNA cytochrome c oxidase subunit I. Larvae of Psettina tosana have anterior-most two elongated dorsal fin rays. Uniserial melanophores present on the dorsal and anal fin base, whereas melanophores on the body absent. An inflection point in the relative growth of head length and head depth against SL was shown between 9.93 mm and 10.73 mm SL. The examined larvae of Psettina tosana are clearly distinguished from the most similar species, Psettina iijimae in having no melanophore patches in the proximity of dorsal and anal fin base.

Design of Broadband Polarization Diversity Antenna for Mobile Base Stations (이동 통신 기지국용 광대역 편파 다이버시티 안테나 설계)

  • Seo, In-Jong;Cho, In-Ho;Lee, Cheon-Hee;Jung, Jin-Woo;Lee, Hyeon-Jin;Lim, Yeong-Seog
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.21 no.9
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    • pp.1023-1029
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    • 2010
  • In this paper, we proposed the broadband polarization diversity antenna operating in the PCS, WCDMA and WiBro band for mobile base station. We designed the antenna using the dipole antenna of the square loop type and microstrip feeding structure. Additionally, we used the choke box to remove the distortion of radiation patterns by the reflector structure when operating broadband. The simulation was performed using MWS in a commercial tool of CST company and the antenna was fabricated on a teflon substrate with 3.33 of the relative permittivity. The proposed antenna has the bandwidth of 640 MHz(from 1.75 to 2.39 GHz) when VSWR is below 1.5. At the operating bands, the interisolation between the cross-pair radiators is less than -25 dB and the maximum gains for PCS, WCDMA and WiBro band are 8.9, 8.2 and 8.6 dBi, respectively.

Genetic identification of Sinomenium acutum based on chloroplast gene ndhF sequences

  • Ryuk, Jin Ah;Lee, Hye Won;Ko, Byoung Seob
    • The Korea Journal of Herbology
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    • v.28 no.5
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    • pp.1-6
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    • 2013
  • Objectives : This study was conducted to identify the original Sinomini Caulis et Rhizoma plant among Stephania tetrandra, Cocculus trilobus, and Aristolochiae fangchi to develop the genetic marker for Sinomini Caulis et Rhizoma. Methods : Sinomenium acutum was identified by the classification and identification committee of the National Center for Standardization of Herbal Medicines. The chloroplast ndhF gene was amplified. We performed sequences alignment analysis of Sinomenium acutum, Stephania tetrandra, C. trilobus, and A. fangchi using BioEdit program. The SFR markers designed were consisted of SF01, SR04, and SR05 primers. Results : Many variations of Sinomeni Caulis et Rhizoma are currently commercialized as herbal medicine. We compared the base sequences of the ndhF intergenic space of chloroplast DNA with Sinomenium acutum, Stephania tetrandra, C. trilobus, and A. fangchi. According to the results, it showed that the nucleotide variations were seen in 30 genes of four species. Phylogenetic analysis revealed that 4 species were classified into five groups based on an inter-group divergence in nucleotide sequence of 9%. We developed SFR marker nucleotides enough to authenticate respective species and confirmed its application on the band size at 419 base pair. These sequence differences at corresponding positions were available genetic markers to identity the Sinomeni Caulis et Rhizoma. Conclusions : Base on these results, the ndhF region was effective in distinguishing Sinomini Caulis et Rhizoma The SFR genetic marker was useful for identifying Sinomini Caulis et Rhizoma with other species.

A Comparative Study of Acidemia-induced Hyperkalemia and Hyperkalemia-induced Acidemia (산혈증 유발 고칼륨혈증과 고칼륨혈증 유발 산혈증의 비교 연구)

  • Yoon, Jun-Oh;Park, Choon-Ok;Hwang, Sang-Ik;Kim, Chong-Whan;Kim, Woo-Gyeum
    • The Korean Journal of Physiology
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    • v.24 no.1
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    • pp.123-129
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    • 1990
  • A comparative study of acid-base balance has been made between acidemia-induced hyperkalemia and hyperkalemia-induced acidemia. A group of rabbits was infused 0.1 N hydrochloric acid solution and metabolic acidosis was induced. Another group was administered 20 mM potassium chloride solution and hyperkalemia was induced. The third group was infused 0.1 N hydrochloric acid and 20 mM potassium chloride solution, simultaneously. Acid-base data and plasma potassium ion concentration were monitored every thirty minutes in these three groups of rabbits. Following results were obtained: 1 ) Along with the infusion of hydrochloric acid, acute metabolic acidosis was induced in the rabbits. Plasma bicarbonate ion concentration decreased primarily in this group. As a respiratory compensation, there was a tendency of reduction of arterial $Pco_{2}$. The alteration of data became larger along with the amount of administration and the time elapsed. However, hyperkalemia was not so severe compared with the second group. 2) In potassium chloride infused group, plasma potassium ion concentration increased along with the time elapsed and the amount of infusion. And the alteration of acid-base data was parrallel to the level of potassium ion concentration, above all depression of pH was prominent. 3) Above data suggest that when acute metabolic acidosis was induced, exchange of intracellular potassium ion with extracellular hydrogen ion seems significant for the regulation of extracellular acid-base balance. And when hyperkalemia was induced with the infusion of potassium chloride solution, the exchange of intracellular hydrogen ion with extracellular potassium ion also seems significant for the regulation of extracellular potassium balance. 4) In the group of rabbits infused hydrochloric acid and potassium simultaneously, disturbances of acid-base balance and potassium balance were much more severe than two other groups. In these mixed disturbances, the process of compensatory mechanism might be inhibited and one disturbance might aggregate each other. 5) Through above data it has been postulated that in acid-base disturbance potassium balance can be sacrificed as a compensatory mechanism, and vice versa in disturbance of potassium balance. And our data also suggest that hydrogen ion and potassium ion are compensatory pair, one another.

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A new Family of Poecilostomatoid Copepods (Polyankyliidae) from a Tide Pool on Mud Flat in Korea

  • Ho, Ju-Shey;Kim, Il-Hoi
    • Animal cells and systems
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    • v.1 no.3
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    • pp.429-434
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    • 1997
  • A new genus with a new species of poecilostomatoid copepod, Polyankylis orientalis, is described based on a pair of specimens collected from a tide pool on muddy shore in Korea. It represents a new family of the lichomolgoid complex with the following distinguishing characters: (1) 6-segmented antennule (resulted from the fusion of the third and fourth segments) with additional aesthetascs in male, (2) hook on mediodistal corner of antennal coxobasis (first segment), (3) proximal and distal scales on the outer margin of mandible present, (4) main axis of mandibular gnathobase short and lacking notches at the base of mandibular lash, and (5) inner margin of mandibular lash with denticulate processes. Additionally, maxillar syncoxa carries a large, pointed process on ventral surface, legs 1-4 do not show sexua1 dimorphism, and leg 4 is uniramous with 1-segmented exopod. A detailed discussion is given of its affinities with the ten existing families of the lichomolgoid complex.

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On a Study of Measurement Method of Utterance Velocity for the Reduction of Transmission Rate in CELP Vocoder. (LSP 파라미터를 이용한 발성측정법)

  • 장경아;배명진
    • Proceedings of the IEEK Conference
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    • 2000.11d
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    • pp.199-202
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    • 2000
  • Speaking Rate has variety depends on the situation and habit of speakers. It has been many studied about speaking rate In speaker recognition. The study of speaking rate in speech recognition is one of considerable matter when It is recognized the speakers and it is measured by many speech data base and complicate estimation for accuracy. In this paper, conventional vocoder process the speech signal when encoding and transmitting without regard to speaking rate so in order to apply the speaking rate for vocoder It should be considered the simpler algorithm and less computation amount than the conventional method of speaking rate used In speech recognition. We proposed the speaking rate algorithm which is used the simple parameter with Line Spectrum Pair (LSP). The proposed peaking rate method is measured by the information of LSP in speech. We measured the variety rate of phenomenon about utterances which have different velocity, respectively. As a result, It has distinct variation rate of phenomenon between utterances uttered fast and slow and the rate is 42.8% higher in case of uttered fast than in case of uttered slow.

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New Record of Brama dussumieri (Pisces: Bramidae) from Korea, as Revealed by Morphological and Molecular Analyses

  • Lee, Woo Jun;Kim, Jin-Koo
    • Fisheries and Aquatic Sciences
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    • v.18 no.3
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    • pp.311-316
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    • 2015
  • Ten specimens of Brama dussumieri (family Bramidae) were collected from waters off Jeju Island, Busan, and Gangneung, Korea, during 2013-2014. The specimens were characterized by having 58-64 lateral line scales and 13-15 gill rakers. An analysis of 567 base pair sequences of mitochondrial DNA cytochrome c oxidase subunit I showed that sequences in our ten specimens are concordant with those of B. dussumieri from the USA, India, and Japan, although with slight differences (genetic distance = 0.000-0.018). Brama dussumieri was distinguished from the most similar species, Brama japonica, by the number of lateral line scales (57-65 in B. dussumieri vs. 65-75 in B. japonica) and the number of gill rakers (13-15 in B. dussumieri vs. 17-20 in B. japonica). We propose the new Korean name "Wae-sae-da-rae" for B. dussumieri in Korea.

First Record of the Omura's Whale (Balaenoptera omurai) in Korean Waters

  • Kim, Ji Hye;Kim, Hyun Woo;Kim, Eun-Mi;Sohn, Hawsun
    • Animal Systematics, Evolution and Diversity
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    • v.34 no.3
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    • pp.162-167
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    • 2018
  • To confirm the genetic identification and phylogenetic relationships of unidentified 6 baleen whales by-caught from 2002 to 2016, a partial sequence of approximately 500 base pair (bp) of the mitochondrial DNA (mtDNA) control region was analyzed and compared to published sequence from Genbank. Our results indicated that the two individuals among 6 specimens are clustered with Omura's whale clade through phylogenetic analysis, which had only a single haplotype. Omura's whale was reclassified as a new species in 2003 and they had not been previously reported in Korean waters. This study firstly revealed existence of Omura's whale in Korean waters by molecular analysis based on mtDNA control region.

Evidence gathering for line based recognition by real plane

  • Lee, Jae-Kyu;Ryu, Moon-Wook;Lee, Jang-Won
    • 한국HCI학회:학술대회논문집
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    • 2008.02a
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    • pp.195-199
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    • 2008
  • We present an approach to detect real plane for line base recognition and pose estimation Given 3D line segments, we set up reference plane for each line pair and measure the normal distance from the end point to the reference plane. And then, normal distances are measured between remains of line endpoints and reference plane to decide whether these lines are coplanar with respect to the reference plane. After we conduct this coplanarity test, we initiate visibility test using z-buffer value to prune out ambiguous planes from reference planes. We applied this algorithm to real images, and the results are found useful for evidence fusion and probabilistic verification to assist the line based recognition as well as 3D pose estimation.

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