• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.45-45
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• 2020

A large number of expressed sequence tags (ESTs) in public databases have provided an opportunity for the systematic development of simple sequence repeat (SSR) markers. EST-SSRs derived from conserved coding sequences show considerable cross-species transferability in related species. In the present study, we assessed the utility of foxtail millet EST-SSRs in barnyard millet. A total of 312 EST-SSRs of foxtail millet were tested using 84 Echinochloa crus-galli germplasm accessions; a high rate of transferability (62%) and 46 primer sets (13%) were shown the polymorphism in barnyard millet. The 13% of functional EST-SSRs) was demonstrated between cereals and barnyard millet. SSR marker profile data were scored for the computation of pairwise distances as well as a Neighbor Joining (NJ) tree of all the genotypes. The averaged values of gene diversity (H_E) and polymorphism information content (PIC) were 0.213 and 0.179 within populations, respectively. The 84 barnyard millet germplasm accessions were divided into five different groups, which agreed well with their geographical origins. The exotic 12 accessions of India type barnyard millet (E. frumentacea) were all separated form Korean local collection genotype. The present results provide evidence of divergence between cultured and wild type barnyard, as a millet and grass. The polymorphic SSR markers indicated in this study were of great value in analysis of genetic diversity that can be further used for crop improvement through breeding.

• Journal of The Korean Society of Grassland and Forage Science
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• v.41 no.3
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• pp.217-222
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• 2021

Barnyard millet is a short-lived tropical, short-term C4 plant and has superior vitality in humid conditions owing to its freshwater habitat. It shows strong adaptability to soils with poor drainage and low fertility, and efficiently competes with rice in paddy fields. Barnyard millet grain is used as feed in the Indian region and is a great source of dietary fiber, proteins, fats, vitamins, and some essential amino acids. Considering its high nutritional value and its potential as a food resource and fodder crop, various countries are showing interest in cultivating barnyard millet. However, in Korea, farm households have not yet recognized the benefits of cultivating barnyard millet, and research regarding this is scarce. In this review, the features of forage barnyard millet and its related research trends are discussed, with the aim of improving interest in this crop and promoting its cultivation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.5
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• pp.466-476
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• 1993

Field experiment was carried out to study the new and old leaf development and photosynthesis of sorghum-sudangrass hybrid 855F, pearl millet Suwon No.6 and barnyard millet in cutting and non-cutting plots from the 2nd cutting day(September, 17th). Leaf regrowth of sorghum-sudangrass hybrid and pearl millet begun after the 2nd cutting day, except barnyard millet. Photosynthetic rate of new leaf blades in cutting plots reached to higher level than old leaf at 12th to 20th day after cutting(DAC). Stomatal density of leaf blade of each crop in non-cutting plot was observed higher pearl millet and sorghum-sudangrass hybrid than barnyard millet. New leaf blade of each crop in cutting plot was showed complete development in stomata size, form and vascular bundles in 12th DAC. Non-structural carbohydrates(NSC) contents of stembase in sorghum-sudan hybrid which had 17 %, the highest among three forages decreased daily into 8th DAC and begun to increase from 20th DAC. But, those of pearl millet and barnyard millet in cutting plots were about 10 % at cutting day and inclined to decrease continuously into the 20th DAC and reached about 3～4% in 32nd DAC. These results showed that regrowth energy of sorghum-sudangrass hybrid was mainly dependent on non-structural carbohydrates of stembase until 12th DAC, but pearl millet had active lower leaves supported its regrowth by concurrent photosynthesis. As barnyard millet which did not reserve enough NSC, its regrowth fail to survive under low temperature. Also, late regrowth of rest two forage crops was delayed with decreasing daily temperature after mid-September.

• Journal of The Korean Society of Grassland and Forage Science
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• v.42 no.3
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• pp.176-182
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• 2022

The aim of this study was to investigate dry matter productivity and nitrate nitrogen content in the growth stages of barnyard millet (Echinochloa esculenta) cultivated for feed, which was treated with different nitrogen fertilization levels. An early variety of barnyard millet (cv. Shirohie) was used for the test, and the different treatments with nitrogen fertilizer were as follows: 50% (N-40 kg/ha, T1), 100% (N-80 kg/ha, reference amount, T2), 150% (N-120 kg/ha, T3), 200% (N- 160 kg/ha, T4), 250% (N-200 kg/ha, T5), and 300% (N-240 kg/ha, T6). Sowing was done on May 13, 2021 and plants were harvested for four stage; vegetative stage, elongation stage, heading stage, and milk stage. The length of the millet increased significantly as the amount of nitrogen fertilization increased during the harvest period (p<0.05), but the difference was insignificant during the milk stage (p>0.05). Moreover, barnyard millet dry matter yield increased significantly as the levels of nitrogen fertilization increased (p<0.05), but there was no significant difference in dry matter yield among nitrogen fertilization levels during the heading stage (p>0.05). Chlorophyll also was significantly higher in T5 (250%) at all harvesting times, whereas nitrate nitrogen content was highest at the vegetative stage, gradually decreased as growth progressed, and lowest at the milk stage. Finally, as the nitrogen fertilization levels increased, the nitrate nitrogen content was significantly higher in all treatment groups (p<0.05). Therefore, our results suggest that the most appropriate nitrogen fertilizer levels is between 150%-200%, considering the dry matter yield, feed ingredients and nitrate nitrogen content in barnyard millet for feed.

• Journal of The Korean Society of Grassland and Forage Science
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• v.41 no.3
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• pp.205-209
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• 2021

Barnyard millet (Echinochloa esculenta) can be processed through soiling, hay, and silage, depending on the weather conditions during harvesting. However, research on barnyard millet is insufficient, and standards for cultivar, seeding density, and fertilizers have not been established. This study was conducted to examine the effects of seeding density and seeding methods on dry matter yields. For this, we used the early-maturing (Shirohie millet) variety of the barnyard millet. The experimental design included different seeding density (10 kg/ha, 15 kg/ha, 20 kg/ha (standard seeding density), 25 kg/ha and 30 kg/ha), and different methods of seeding (drill seeding and broadcast seeding). The seeding date was May 13, 2021, and the harvest date was July 13, 2021. Harvesting was carried out when the heading reached 40 %. Lodging occurred at 5, 9 and 7 at 20, 25 and 30 kg/ha densities in the broadcast seeding, but not in the drill seeding. With decreasing density of seeding, tillage number showed an increasing trend in both drill seeding and broadcast seeding (p>0.05). The plant heights were comparable in both drill seeding and broadcast seeding (p>0.05). The heading stage of barnyard millet was checked July 7 for drill seeding, and, on July 8 for broadcast seeding. It took 62 days, i.e., till July 13 for the heading to reach 40 % of the output. The dry matter yield of barnyard millet was significantly higher at the seeding density of 30 kg/ha, for both the methods of seeding (p<0.05). There was no difference in the chemical composition of grain, based on the seeding method and seeding density. However, as the seeding density increased, the CP, NDF, ADF, and TDN contents increased in both drill seeding and broadcast seeding. We found that, the dry matter content was the highest at the seeding density of 30 kg/ha for both the methods of seeding, but this was only 1.3 times higher than that of 10 kg/ha. Considering the seed price and labor force involved in seeding, it is advisable to have a seeding density of 15-20 kg/ha.

• Weed & Turfgrass Science
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• v.6 no.2
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• pp.124-129
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• 2017

The Experiments were conducted by moderate season culture of each of early, medium and late maturing varieties which were considered to be of strong salt tolerance in low and high salty reclaimed areas (0.2% at the May). This study was carried out to investigate the proper nitrogen fertilizer level and seeding rates at reclaimed saline land in Korea. The proper seeding rates were $40kg\;ha^{-1}$ in 0.2% saline land. The dry matter production of barnyard millet was possibly estimated by exponential functions of $Y=0.0098X^2+0.7030X+2.6267$. Effects of nitrogen rate on agronomic characteristics, forage yield, and chemical composition of barnyard millet to reclaimed tidal land are summarized as follows: The proper nitrogen fertilizer level was $200kg\;ha^{-1}$ in 0.2% saline land. N was absorbed actively before the emergence of the barnyard millet but showed relative decrease thereafter. The early growth of the barnyard millet was inhibited, resulting in the favorable late growth, increased panicle weight and ratio of matured grain. These results suggest that barnyard millet is the most forage crops for cultivation on reclaimed tideland in view of the good emergence and forage production.

• The Korean Journal of Food And Nutrition
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• v.25 no.4
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• pp.706-712
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• 2012

The effects of barnyard millet (Echinochloa spp.) content (10~30%) on wheat flour dough and noodle properties were investigated. As the amount of barnyard millet increased, the particle size and ash content of mixed flour increased, while lightness tended to decrease. The gelatinization characteristics of millet flour showed higher peak viscosity, holding strength, final viscosity, and setback compared with wheat flour. There was significant positive correlation between protein content and sedimentation volume, as well as between protein content and water absorption. As the amount of barnyard millet increased, hardness, springiness and cohesiveness of wet noodles tended to decrease. From the results of sensory evaluation, composite flours (addition up to 20% barnyard millet) were rated with a quality score for taste and overall acceptance which was comparable with the control flour.

• Journal of The Korean Society of Grassland and Forage Science
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• v.42 no.1
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• pp.26-31
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• 2022

This study was carried out to evaluate the growth characteristics and forage yield potential for warm season grass as emergency forages. The experimental design was a randomized block design (RBD) with three replications. Two barnyard millet (Echinochloa species cv. Shirohie and Jeju native), a pearl millet (Pennisetum glaucum cv Feed milk 2) a proso millet (Panicum miliaceum cv Native), a teffgrass (Eragrostis tef cv. Tiffany) and a kleingrass (Panicum coloratum cv. Selection 75) were compared for forage production and quality at the Mid regions of Korea. Warm season forage crops were sown on May 21 and June 23 respectively, and in 2021, it was sown twice on May 21 and June 21 The number of days to seedling emergence for barnyard millet and teffgrass was observed approximately 10 and 3 days after seeding, respectively. The cultivation period from seeding to harvest was within 60 days for all entry spices except for the late-heading type barnyard millet (within 84 days). As for the dry matter yield by seeding date, the dry matter yield of the late-heading type barnyard millet in May seeding was the highest at 23,872 kg/ha, and the kleingrass was the lowest at 3,888 kg/ha. For the June seeding, the dry matter yield of the late-heading type barnyard millet was 17,032 kg/ha, the highest, and the proso millet, teffgrass and kleingrass showed the lowest at 5,468, 5,442, and 5,197 kg/ha, respectively. The crude protein (CP) content was varied by warm season grass species, but the early-heading type barnyard millet, teffgrass, and kleingrass showed the highest tendency, and the late-heading type barnyard millet showed the lowest at 5.7~5.9%. Neutral detergent fiber (NDF) and acid detergent fiber (ADF) content did not show a significant difference between the seeding in May, but kleingrass in June sowed lower than the others.

• Journal of The Korean Society of Grassland and Forage Science
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• v.41 no.2
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• pp.141-146
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• 2021

This study was evaluated to compare annual productivity and feed value of Italian ryegrass and summer forage crops at paddy field in middle region of Korea. Italian ryegrass (Kowinearly) was used as winter forage crop, and forage rice (Youngwoo) and barnyard millet (Jeju) were used as summer forage crops. Each crop was cultivated using the standard forage cultivation method. The plant height, dry matter yield, crude protein content, and total digestible nutrient content of Italian ryegrass were 90.6 cm, 7,681 kg/ha, 9.2%, and 63.8%, respectively, and it was no significant difference by summer forage crops (p>0.05). The plant height of summer forage crops was the higher in barnyard millet than in forage rice (p<0.05). The dry matter, crude protein, and total digestible nutrient yields of summer forage crops were the higher in forage rice than in barnyard millet (p<0.05). Also, the feed value of summer forage crops was higher in forage rice than in barnyard millet. In conclusion, the combination of Italian ryegrass-forage rice was the most effective cropping system for annual forage production with high-yield and high-feed value, and it was considered the combination of Italian ryegrass-barnyard millet was good cropping system for annual forage production through reducing labor and cultivating stable at paddy field in middle region of Korea.

• Journal of Life Science
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• v.25 no.11
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• pp.1265-1272
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• 2015

Barnyard millet Miryang 3 [Echinochloa esculenta (A. Braun)] grains have recently been acknowledged for beneficial health properties due to phenolic ingredients and dietary fiber. This study has been conducted on the anti-diabetic activity of barnyard millet Miryang 3 which shows the strongest anti-inflammatory activity among barnyard millet inhabiting in South Korea. When 80% ethanol (EtOH) extract of barnyard millet Miryang 3 grains were orally administered into db/db diabetic mice for 8 weeks (600 mg/kg/day), the glucose level in blood following fasting appeared to be improved compared to the control group. The results of glucose tolerance test and blood lipid profile assay were similar to those of the metformin-administered positive control group. In addition, the level of body weight increase (8.54±2.24) was lower than the level of metformin-administered group (10.36±3.15); however, there was no subtle difference with negative and positive control groups in terms of food efficiency rates. In addition, total cholesterol levels of the 80% EtOH extract-administered group (160.7±7.6) were significantly reduced compared to the diabetic control group (229.3±47.8) and metformin-administered group (176.0±25.6). Consequently, these results show that barnyard millet grains alleviates many of the diabetic symptoms in vivo non-insulin-dependent diabetes mellitus, and suggest that barnyard millet grains can be applicable in developing new functional food materials.