• KOREAN JOURNAL OF CROP SCIENCE
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• v.57 no.2
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• pp.171-181
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• 2012

Lysine is the first limiting essential amino acid in cereals for humans and monogastric animals, although its content is generally low. A chemically induced high-lysine barley mutant, M98, has an agronomically undesirable shrunken endosperm trait. In order to obtain detailed insight into the atypical traits of M98 grains, we characterized amino acid composition and protein profiles of M98 and its parent cultivar Chalssalbori. Among a total of 16 amino acids, the percentage of each of the 7 amino acids, including lysine, was 1.2~1.8 times higher in M98, comparing to Chalssalbori. The percentage of proline and its precursor, glutamic acid, in M98 was about the half of that of the amino acids in Chalssalbori, but arginine synthesized from glutamic acid was 1.8 times higher in M98, compared that in the parent cultivar. Theses results indicated that the mutation in M98 grains might alter the proportion of amino acids linked to each other in a biosynthetic pathway. A comparison of grain proteome profiles between Chalssalbori and M98 revealed 70 differentially expressed protein spots, where 45 protein spots were up-regulated and 25 protein spots down-regulated in M98 compared to those in Chalssalbori. Of these changed protein spots, 53 were identified using nano-electrospray ionization liquid chromatography mass spectrometry. Most of these identified proteins were involved in various biological processes. In particular, 28 protein spots such as ${\beta}$-amylase, serpins and B3-hordein were identified as proteins associated with the atypical traits of M98. It was thought that a genetic study on the unique protein profile of M98 would be needed to develop an agronomically feasible barley cultivar with high-lysine trait.

• The Plant Pathology Journal
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• v.34 no.2
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• pp.104-112
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• 2018

Accurate and rapid detection of bacterial plant pathogen is the first step toward disease management and prevention of pathogen spread. Bacterial plant pathogens Clavibacter michiganensis subsp. nebraskensis (Cmn), Pantoea stewartii subsp. stewartii (Pss), and Rathayibacter tritici (Rt) cause Goss's bacterial wilt and blight of maize, Stewart's wilt of maize and spike blight of wheat and barley, respectively. The bacterial diseases are not globally distributed and not present in Korea. This study adopted comparative genomics approach and aimed to develop specific primer pairs to detect these three bacterial pathogens. Genome comparison among target pathogens and their closely related bacterial species generated 15-20 candidate primer pairs per bacterial pathogen. The primer pairs were assessed by a conventional PCR for specificity against 33 species of Clavibacter, Pantoea, Rathayibacter, Pectobacterium, Curtobacterium. The investigation for specificity and sensitivity of the primer pairs allowed final selection of one or two primer pairs per bacterial pathogens. In our assay condition, a detection limit of Pss and Cmn was $2pg/{\mu}l$ of genomic DNA per PCR reaction, while the detection limit for Rt primers was higher. The selected primers could also detect bacterial cells up to $8.8{\times}10^3cfu$ to $7.84{\times}10^4cfu$ per gram of grain seeds artificially infected with corresponding bacterial pathogens. The primer pairs and PCR assay developed in this study provide an accurate and rapid detection method for three bacterial pathogens of grains, which can be used to investigate bacteria contamination in grain seeds and to ultimately prevent pathogen dissemination over countries.

• Journal of the Korean Society of Food Science and Nutrition
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• v.27 no.4
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• pp.789-799
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• 1998

Literatures on microorganisms of traditional Korean nuruk published since 1945 were reviewed in this paper. Traditional Korean nuruk consists of raw barley and various grains. Traditional Korean nuruk consists of unbolied raw barely and various grains. They are ground to paste and moistened, and then naturally inoculated by airborne microorganisms. Therefore, many kinds of microorganisms such as fungi, yeast, and bacteria grwo in nuruk. Since 1945, new 14 species of Aspergillus and 9 species of Penicillium have been identified from traditional Korean nuruk. Total number of fungal species identified so far is now up to 38 species among 12 different genus. Among newly isolated fungal species, Aspergillus penicilloides and Penicillium, expansum showed not only high production rate of acid and amylase but also extreme stability of the enzyme at room temperature for 3 months. As examples of newly isolated yeast species, there are 5 species of Candida, 4 species of Hansenula, 1 species of Pichia and 1 species of Schizosaccharomyces. Total number of yeast species isolated so far is up to 18 species from different 8 genus. Newly isolated bacteria, were Bacillus pumilus, Lactobacillus casei and Leuconostoc mesenteroides.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.3
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• pp.299-303
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• 1992

Methods for harvesting and storage of rice were also originally introduced from the ancient China like other practices of agricultural techniques. Thus, optimum time or rice harvesting was adopted by appropriate draining to prevent the losses, and efficient tools were utilized for harvesting and manufacturing. In addition, our unique and original techniques for rice storage were learned and handed down, which prevented winds and rains, and allowed good ventilation. In the 15th and 16th centuries, treatment of smartweed extracts as emphasized to prevent insect damage for stored barley and rice cutting immediately at harvest was encouraged to prevent shattering of rice grains. In the 18th and 19th centuries, means for prevention of damages by water, insects and rats when grains stored in the field were suggested. Also, studies for ecology of various rice cultivars and storage techniques for a long period of time were necessarily emphasized to assume the optimum time of harvest regionally and to cooperatively work timely.

• Korean Journal of Soil Science and Fertilizer
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• v.5 no.1
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• pp.17-23
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• 1972

The effect of completed mineral fertilizer on barley was investigated on ordinary upland and newly recrimed soil derived from granite, basalt and shale. 1. Yield of barley grains with application of completed meneral fertilizer 1 level varied from 100.8% to 233.1% on various soils in different parents rock, and 2 levels of the fertilizer varied from 250.1% to 309.5% on ordinary upland and newly recrimed soil derived from granite compared with control plot. But no effect on newly recrimed soil derived from basalt and shale. 2. $P_2O_5$, MgO and B contents in straw were increased, but Mn contents was decreased on all of soil. N contents in straw was decreased on the soil derived from granite, but $K_2O$ contents was increased on the soil derived from basalt and shale. 3. Relation between the yield and absorbed nutrient had related with positive correlection of 1-5%. 4. N and Mn contents in soil were decreased by application of completed mineral fertilizer, but soil pH, C.E.C. $Av-P_2O_5$, Ex-CaO, Ex-MgO and B contents were increased. Especially soil pH and C.E.C. were increased remarkably. 5. Relation between the yield and Ex-CaO and Ex-MgO contents in soil had related with positive correlation, but Mn contents had related with negative correlation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.5
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• pp.425-431
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• 1993

Temperature effects on the grain filling of winter barley(CV. Gangbori, Olbori and Suwon 18) were studied in controlled environments under three day /night temperature regimes (viz. 25/20, 20/15 and 15/10$^{\circ}C ) and at three stages of development, viz. Vegetative, spike development and grain filling stages. Physiological maturity times to the temperature regimes were observed at 53 to 57 days after heading at 15/10$^{\circ}C , 43 to 45 days and 32 to 33 days at 20/15$^{\circ}C and 25/5$0^{\circ}C$, respectively. When plants were grown in 25/20$^{\circ}C during the spike development stages, the number of spikes per plant and grains per spike decreased by 39% and 41% respectively compared with 20/15$^{\circ}C of day / night temperature. The effect of high temperature on grain weight was not significant in all growth phases except post-anthesis where grain weight decreased by 30%. Therefore, the most important temperature effects were round during the spike development stages.

• Applied Biological Chemistry
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• v.44 no.1
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• pp.30-34
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• 2001

Physicochemical properties of milled rice, brown rice, pearled barley, wheat, sorghum, foxtail millet, soybean, and adzuki bean were evaluated for the production of extrusion formed multi-grain. Grain flours showed large differences in chemical composition including starch, protein, fat, ash, and total dietary fiber contents. Grain flours were brighter in the following order of milled rice>pearled barley>soybean>brown rice>wheat>sorghum>adzuki bean>foxtail millet, and most of the grain flours showed red-yellowish color. Mean particle sizes of grain flours were different among various grains, and whole grain flours tended to have coarser particle size than milled grain flours. The amounts of damaged starch in cereal flours were varied from 5.4 to 10.9%, and limited amount of damaged starch was present in legume flours. Water absorption index of grain flours was, in decreasing order, adzuki>bean>milled rice>brown rice>sorghum>wheat>foxtail millet>pearled barley>soybean. Water solubility index was higher in legume flours containing high protein content.

• Korean Journal of Food Science and Technology
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• v.16 no.1
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• pp.51-58
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• 1984

Grains of naked barley (Baekdong cultivar) were polished, powdered and subjected to the successive extraction into free and bound liquid fractions. These were further fractionated into lipid classes and quantified by means of thin layer chromatography, column chromatography and gas-liquid chromatography. Contents of free and bound lipids in barley flour were 2.27% and 1.01%, which were decreased to 2.12% and 0.76%, respectively, after purification. Free and bound lipids were consisted of monoglycerides, diglycerides, triglycerides, free sterols, sterol esters, free fatty acids and polar lipids. Major constituents of free lipids were 56.2% triglycerides, 14.9% free fatty acids and 13.4% sterols while those of bound lipids were 73.8% polar lipids, 8.4% free fatty acids and 5.2% triglycerides. The content of non-polar lipids in free lipids was 93.6% as compared with 26.2% in bound lipids. However, phospholipids content in bound lipids was 55.5% as compared with 2.5% in free lipids, and glycolipids content in bound lipids was 19.4% as compared with 3.9% in free lipids. Major fatty acids in the free and bound lipid fractions were linoleic acid 52.1%, 54.8%, palmitic acid 24.8%, 30.0% and oleic acid 15.6%, 8.8%, respectively, showing similar patterns in both fractions. The amount of unsaturated fatty acids in free lipids was 72.8% as compared with 68.0% in bound lipids. In comparing the fatty acid composition of non-polar lipids, glycolipids and phospholipids, no difference was observed between free and bound lipid fractions while a slight difference was found among the lipid constituents.

• Korean Journal of Breeding Science
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• v.50 no.4
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• pp.516-521
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• 2018

"Nulichal" was developed by the National Institute of Crop Science, RDA, in 2010. The initial cross was conducted in 2000, and an elite line (HB15305-B-B-31-2) was selected in 2005. Subsequently, preliminary yield and advanced yield trials have designated it as "Iksan 95." It showed good agronomic performance in regional yield trials from 2008 to 2010 and was released as "Nulichal," with resistance against the barley yellow mosaic virus (BaYMV) and high yields with a waxy endosperm. The average heading and maturation dates of "Nulichal" were April 24 and May 30, respectively in paddy fields, which were 1 day later than those of the check cultivar "Saechalssalbori." It had culm and spike lengths of 82 and 4.0 cm, respectively. It showed $774spikes/m^2$, 63 grains/spike, 25.0 g of 1,000-grain weight, and 793 g of test weight. "Nulichal" showed higher levels of resistance to BaYMV than the naturally occurring Iksan (type III strain), Naju (type I), and Jinju (type II). The expansion rate was higher than that of "Saechalssalbori" and its hardness, gumminess, and chewiness were lower than those of "Saechalssalbori." Its average pearled grain yield in the regional yield trial was 4.00 MT/ha in paddy fields, which was 10% higher than that of the check cultivar. The combined availability of "Nulichal" was improved by its increased culm length compared with that of "Hinchalssal."

• Korean Journal of Food Science and Technology
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• v.53 no.3
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• pp.245-251
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• 2021

We determined the quality characteristics, acrylamide concentration, and antioxidant activity of Nurungji prepared using white rice, brown rice, gamma-aminobutyric acid (GABA) brown rice, oats, and barley. The moisture content of Nurungji prepared using white rice was the lowest (1.48%) and the highest (6.53%) was obtained in barley Nurungji. The brightness (L^*) of white rice Nurungji was the highest, whereas that of GABA brown rice Nurungji was the lowest. The acrylamide concentration was the lowest (37.24 ㎍/g) in white rice Nurungji and the highest (255.50 ㎍/g) in oats Nurungji. The levels of total polyphenol and total flavonoid were the lowest in white rice Nurungji and high in oats and GABA brown rice Nurungji. The antioxidant activity was higher in Nurungji prepared using oats, GABA brown rice, brown rice, and barley than that using white rice. It is necessary to select an appropriate grain when preparing Nurungji by considering the amount of acrylamide produced.