• 한국응용곤충학회지
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• 제27권3호
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• pp.138-143
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• 1988

한국산 긴나무좀과와 나무좀과의 동정에 도우믈 주고자 체계적으로 아과, 족, 속에 대한 검색표를 마련하였으며 미 기록 속인 Scolytogenes 가 추가 되었고 전체적인 형태, 촉각등도 도시하였다.

• 한국식품영양과학회지
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• 제44권7호
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• pp.1090-1099
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• 2015

본 연구는 잠상산물로부터 고부가가치 항당뇨 기능성 소재를 개발하기 위한 연구의 일환으로, 먼저 뽕나무 부위별 4가지(오디, 뽕잎, 상지, 상백피) 에탄올 추출물의 ${\alpha}$-glucosidase 저해활성을 측정한 결과 상백피가 가장 높은 저해활성을 나타내었으며, 그로부터 여러 column chromatography 및 NMR 기기분석을 통해 4가지 ${\alpha}$-glucosidase 저해제를 분리, 정제 및 동정한 결과는 다음과 같다. 상백피 에탄올 추출물을 Diaion HP-20, silica gel, ODS-A 및 Sephadex LH-20 column chromatography를 실시하여 4가지 화합물[Comp. 1(10 mg), Comp. 2(9.6 mg), Comp. 3(9.3 mg), 및 Comp. 4(6.5 mg)]을 분리 및 정제하였으며, 그들의 효소 저해활성을 측정한 결과 Comp. 1($IC_{50}=5.22{\mu}g/mL$), Comp. 2($IC_{50}=1.78{\mu}g/mL$), Comp. 3($IC_{50}=2.94{\mu}g/mL$) 및 Comp. 4($IC_{50}=1.54{\mu}g/mL$)로 나타났다. 그리고 그들 화합물의 동정을 위해 UV 및 NMR spectroscopy를 이용하여 구조를 분석한 결과 Comp. 1(morusin), Comp. 2(kuwanon H), Comp. 3(chalcomoracin A), Comp. 4(chalcomoracin B)로 각각 동정하였다. 이상의 연구 결과로 미루어 보아 상백피로부터 분리된 ${\alpha}$-glucosidase 저해제는 향후 당뇨 치료용 기능성 소재로 활용할 수 있을 것으로 기대된다.

• Journal of Forest and Environmental Science
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• 제37권1호
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• pp.52-61
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• 2021

Decay fungi can decompose plant debris to recycle carbon in the ecosystem. Still, they can also be fungal pathogens, which can damage living trees and/or wood material and cause a large amount of timber loss. We isolated and identified basidiomycetous fungi from the decayed bark of Mongolian oak wrapped with sticky roll traps. The degrading enzyme activities were then tested for all fungal isolates. The decay ability of selected isolates was assessed based on the weight loss of wood discs after inoculating with culture suspension of decay fungi under the different humidity levels. A total of 46 basidiomycetous fungal isolates belonged to 12 species, and 10 genera were obtained from Jong Myo (16 isolates), Chang Kyung palace (7 isolates), Cheong Gye (10 isolates), and Gun Po (13 isolates). Gymnopus luxurians was the most dominant fungus in the present study, and this species distributed in all survey sites with 9 isolates in Jong Myo, followed by 3 isolates in Chang Kyung palace, while Cheong Gye and Gun Po had only 1 isolate each. Among 46 isolates, 44 isolates secreted at least one enzyme, while 25 isolates produced both cellulase and phenol oxidase enzymes, and 2 isolates produced neither. The assessment of decay ability by artificial inoculation indicated that the weight loss of wood discs was significantly influenced by humidity conditions when inoculated with bark decay fungi. The percent weight losses by G. luxurians inoculation in RH of 90-100% and RH of 65-75% were 4.61% and 2.45%, respectively. The weight loss caused by Abortiporus biennis were 6.67% and 0.46% in RH of 90-100% and RH of 45-55%, respectively. The humidity reduction approach should be applied for further studies to control the growth and spread of bark decay fungi on the trunks wrapped with sticky roll traps.

• Applied Biological Chemistry
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• 제39권1호
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• pp.89-94
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• 1996

한방에서 종기, 치질, 위암 및 염증의 치료목적으로 사용되어온 당느릅나무 (Ulmus davidiana Planch) 근피의 메탄을 추출물로부터 sesquiterpene o-naphthoquinone류 화합물 세가지를 분리 정제하였다. 이들의 구조를 UV-vis, IR, EIMS, HR-EIMS 및 여러 가지 NMR 스펙트럼, 특히 새로운 기법인 pulse field gradient (PFG)-HMQC 및 HMBC 스펙트럼의 분석에 의하여 결정하였다. 그 결과 이들은 2,3-dihydro-3,6,9-trimethylnaphtho(1,8-b,c)pyran-7,8-dione, 3,6,9-trimethylnaphtho(1,8-b,c)pyran-7,8-dione 및 2,3-dihydro-4-hydroxy-3,6,9-trimethylnaphtho(1,8-b,c)pyran-7,8-dione으로 동정 되었으며, 각각 mansonone E, F 및 H로 밝혀졌다. 이 화합물들은 당느릅나무에서는 처음 분리되었으며, 특히 mansonone H는 아프리카 열대식물인 Mansonia altissima Chev 및 Helicters angustifolia에서 분리된 후 자연계에서 처음으로 분리되었다. 문헌에 잘못 보고된 mansonone E 와 F의 C-3a, C-6a 및 C-9b의 세 사급탄소의 chemical shift를 최신 PFG-NMR기법에 의하여 동정하여 교정하였다.

• Journal of Ecology and Environment
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• 제37권4호
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• pp.327-339
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• 2014

This research includes the identification and cultivation of aerial algae from 33 sites located in Mt. Gwanggyo of Gyeonggi-do, Korea, from March 2011 to August 2012. The ecological factors of aerial algae were analyzed and a total of 29 taxa were identified in 4 phyla, 5 classes, 11 orders, 15 families, 19 genera, 28 species and 1 variety; 12 taxa of cyanophytes, 8 taxa of chrysophytes, and 9 taxa of chlorophytes were found. As for newly recorded cyanophytes of Korea, Komvophoron jovis, Microcoleus steenstrupii, and Nostoc edaphicum appeared. Komvophoron jovis, previously known to grow on rocks and boulders, appeared in soil. Microcoleus steenstrupii, reported to appear in desert soils, appeared on the wet surface of the soil after rain. Nostoc edaphicum, in symbiosis with fungi, appeared on tree bark as the lichen. Thus, there are a total of 99 reported taxa of Korean aerial algae, including 3 species that were discovered in this study.

• The Plant Pathology Journal
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• 제16권1호
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• pp.48-51
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• 2000

Citrus tristeza virus (CTV) was identified form CTV-infected early satsuma mandarin (Citus unshiu) and yuzu (C.junos) by RT-PCR. The total RNAs were isolated from citrus bark and seaf tissues infected with CTV and reverse transcription was followed with primers designed for amplifying CTV coat protein gene. DNA fragments 738 bp were amplified by RT-PCR and these products were colned for sequence analysis. Based on the sequence analysis, this PCR product has 97% sequence homology to CTV (T-385) CP gene isolated from USA. RT-PCR assay for CTV detection was more sensitivity than ELISA assay which was done with anti-CTV CP antibody. This is the frist report about CTV identification in Cheju island Korea.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1995년도 Proceedings of the Korea Automation Control Conference, 10th (KACC); Seoul, Korea; 23-25 Oct. 1995
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• pp.119-122
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• 1995

Neural network has found wide applications in the system identification, modeling, and realization based on its function approximation capability. THe system governe dby nonlinear dynamics is hard to be identified by the neural network because there exist following difficulties. FIrst, the training samples obtained by the stae trajectory are apt to be nonuniform over the region of interest. Second, the system may becomje unstable while attempting to obtain the samples. This paper deals with these problems in discrete-time system and suggest effective solutions which provide stability and uniform sampliing by the virtue of robust control theory and heuristic algorithms.

• Journal of the Korean Wood Science and Technology
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• 제39권3호
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• pp.213-220
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• 2011

양평 지평향교 대성전의 목부재에 대한 수종 식별과 연륜연대 분석을 통해 정확한 수종과 건축역사를 조사하고자 하였다. 목부재 49점 가운데 연송인 잣나무류로 식별된 창방 5점과 장여 1점을 제외한 43점은 경송인 소나무류였다. 그리고 5개 목부재에 대한 연륜연대가 측정되었는데, 수피가 있는 대보 1점과 종보 1점의 마지막 연도가 각각 1718년과 1720년(만재 형성 완료)으로 측정되어 "학교등록"에 수차례 기록되었던 지평향교 대성전의 이건이 1720년대에 실행되었거나 이건 없이 대규모의 중수가 있었음을 유추할 수 있었다.

• Journal of Plant Biotechnology
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• 제49권2호
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• pp.139-144
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• 2022

The genus Prunus (family: Rosaceae) consists of over 400 plant species and exhibits vast biodiversity worldwide. Given the wide distribution of this genus, its taxonomic classification is important. Anatomical characteristics are conserved and stable and can therefore be used as an important tool for the taxonomic characterization of plants. Therefore, this study aimed to assess and document the anatomical characteristics of the leaf, stem, and seed of P. africana using micrographs and photographs for possible use in the identification, quality control, and phylogenetic analysis of the species. The anatomical sections of a young stem revealed a cortex consisting of isodiametric parenchyma cells, druse crystals, primary vascular bundles, and pith. The mature stem bark majorly consisted of the rhytidome, with the periderm densely arranged in multiple layers; a cluster of stone cells; and sclerenchyma. The leaf sections were hypostomatic, with stomata sizes ranging from 18.90-(22.34)-26.90 × 15.41-(18.40)-21.22 ㎛. The leaf sections showed the presence of characteristic druse crystals, vascular bundles, and mesophyll layers. The pericarp contained the epicarp, mesocarp, and endocarp, with their thickness being approximately 350-400, 300-350, and 30-50 ㎛, respectively. In addition, it contained a seed testa with a thickness of approximately 50-60 ㎛. The morphological and anatomical characteristics observed in P. africana leaves, stems, and seeds in this study could serve as useful data for the taxonomic identification of this species.

• 한국식품영양과학회지
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• 제32권7호
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• pp.1059-1065
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• 2003

우리나라 야산에서 손쉽게 구할 수 있는 구황식물인 목단피를 건조시켜 분쇄한 후 methanol과 여러 용매로 추출하여 식품 부패 미생물의 증식 억제 효과를 검색하고 그 항균물질을 분석하여 다음과 같은 결과를 얻었다. 목단피의 methanol 추출물은 1500$\mu\textrm{g}$/mL 농도에서 B. subtilis, L. monocytogenes, V parahaemolyticus의 증식을 100% 억제하였으며 S. aureus, E. coli도 같은 농도에서 각각 36.31%와 67.81%의 억제 효과를 보였다. 목단피의 ethylacetate 분획 추출물은 낮은 농도인 500$\mu\textrm{g}$/disc의 농도에서 식품 부패 미생물 5종 모두에 대하여 clear zone을 형성하여 항균력을 나타내었다. 특히 L. monocytogenes, E. coli균에 대해서는 낮은 농도인 500$\mu\textrm{g}$/disc에서 각각 12mm, 11mm의 clear zone을 형성하였다. 목단피의 ethylacetate추출물을 silica gel colum chromatography(7cm${\times}$1.2 cm)한 후 thin layer chromatography(TLC)를 실시하여 항균 실험을 한 결과 B. subtilis의 경우 500 $\mu\textrm{g}$/disc 농도에서 2nd fra. 1이 21mm, V. parahaemolyticus는 250$\mu\textrm{g}$/disc 농도에서 14mm를 형성하여 목단피의 methanol 추출물로부터 각 용매별로 계통 분획하여 분리한 ethylacetate 분획으로부터 silica gel column chromatograpy와 TLC를 하여 항균성을 보인 2nd fra. 1의 성분을 알아보기 위하여 HPLC로 단일분리하여 얻은 peak Ⅸ에 대한 compound를 GC-MS로 분석한 결과 peak Ⅸ에서는 isobutyl isopentanoate가 있는 것으로 추정되었다.