• Journal of Korean Society of Water and Wastewater
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• v.20 no.6
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• pp.893-904
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• 2006

To improve sensitivity of biosensor as yeast two-hybrid detection system for estrogenic activity of suspected chemicals, we tested effects of several combinations of the bait and fish components in the two-hybrid system on Saccharomyces cerevisiae inducted a chromosome-integrated lacZ reporter gene that was under the control of CYC1 promoter and the upstream Gal4p-binding element $UAS_{GAL}$. The bait components that were fused with the Gal4p DNA binding domain are full-length human estrogen receptor ${\alpha}$ and its ligand-binding domain. The fish components that were fused with the Gal4p transcriptional activation domain were nuclear receptor-binding domains of co-activators SRC1 and TIF2. We found that the combination of the full-length human estrogen receptor ${\alpha}$ with the nuclear receptor-binding domain of co-activator SRC1 was most effective for the estrogen-dependent induction of reporter activity among the two-hybrid systems so far reported. The relative strength of transcriptional activation by representative natural and xenobiotic chemicals was well correlated with their estrogenic potency that had been reported with other assay systems.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.57 no.3
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• pp.185-193
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• 2021

This study was conducted to evaluate the performance of the octopus pot according to mesh sizes. Entering behavior of Octopus minor and bait (Macrophthalmus japonicus) escape rate on the mesh sizes of the pots were investigated for six times in indoor tank. The sea trials for evaluating the performance of Octopus minor pot to different mesh sizes (22, 20 and 18 mm) were conducted for six times from 2017 to 2018 in the coastal sea of Deukyang Bay, the Republic of Korea. Behavior patterns of contact pot to leaved pot were more frequent than contact pot to bait search. When the octopus contacted to the pot, there was no clear search behavior to distinguish the mesh sizes. Total catch of 46% was accounted for 18 mm pots, followed by 34% at 20 mm and 20% at 22 mm (P < 0.05). Catch per unit effort was calculated as 30 g/pot at 22 mm, 44 g/pot at 20 mm and 59 g/pot at 18 mm. As a result of evaluating 50% selection of mantle length and weight on the mesh sizes, mantle length (mm) and weight (g) were 84.6 and 147.8 in 22 mm, followed by 20 and 18 mm.

• Journal of Food Hygiene and Safety
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• v.8 no.3
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• pp.135-140
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• 1993

Dried fishes such as dried pollack, dried sliced squid, dried white bait, dried anchoy) and dried cod used to cook Korean traditional foods were airpacked in polyethylene tube and irradiated with 7 kGy of gamma-ray source. The effect of gamma irradiation on microbial growth in dried fishes was investigated during storage at ambient temperature for 12 months. The total aerobic bacteria in the control group were contaminated by $3.9{\times}10^{3}\;cells/g$ in dried pollack, $5.6{\times}10^{5}\;cells/g$ in dried sliced squid, $1.2{\times}10^{5}\;cells/g$ in dried white bait, $1.2{\times}10^{4}\;cells/g$ in dried anchovy and $1.2{\times}10^{4}\;cells/g$ in dried cod and 7 kGy irradiation could eliminate the bacteria and also reduce aerobic bacterial load to 1~3 log cycle and no apparent growth of microorganisms occurred during storage. Molds in the control group were contaminated by $4.0{\times}10^{2}\;cells/g$ in dried pollack, $$1.3{\times}10^{2}\;cells/g$ in dried sliced squid, $2.5{\times}10^{2}\;cells/g$ in dried white bait, 90 cells/g in dried anchovy and $2.0{\times}10^{2}\;cells/g$ in dried cod, respectively. 7 kGy irradiation could sterilize the molds and the growth of molds of nonirradiated samples were slightly decreased during storage. Yeasts in the control group were contaminated by $1.4{\times}10^{3}\;cells/g$ in dried poUack, 75 cells/g in dried sliced squid, $1.1{\times}10^{3}\;cells/g$ in dried white bait, 50 cells/g in dried anchovy, $1.6{\times}10^{2}\;cells/g$ in dried cod, respectively and irradiation could sterilize the yeasts and growth of yeasts was slightly decreased in dried pollack and dried white bait but increased in order of dried anchovy, dried cod and dried sliced squid. Coliforms in all sample were sterilized by irradiation and its growth was decreased during storage except dried anchovy.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.20 no.2
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• pp.106-113
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• 1987

The Alaska pollack longline operations, which consist of baiting, shooting, hauling and arrangement of hooks, are dependant on manual labour up to the present. The automation against this traditional way is necessary to eliminate the manual operations and to reduce crew. We have developed a prototype longline system suitable for Alaska pollack longline gear, which is composed of an automatic baiting machine, an automatic line hauler, a hook cleaner and storage rails. The automatic bailing machine driven by hydraulic power is precise baiting method controlled sequentially, and the automatic line hauler is to haul up the mainline by means of hydraulic power and at the same time to split every hook and to carry it onto storage rail automatically. A series functioning tests on shooting and hauling apparatus were carried out in the laboratory and at sea. The results obtained are as follows ; 1. As for the baiting machine, the exciting time of solenoid which operates a directional valve, bait feeding and cutting time, is shortened according to the increase of pressure, and also, after cutting the bait, the over-rotated angle of the blade increased in accordance with the increase of pressure. 2. The baiting efficiency is about $90\%$ when using sand lance (Hypoptychus dybowskii), and the most proper pressure of the hydraulic circuit in feeding and cutting the bait is between $13\;kgf/cm^2\;and\;20\;kgf/cm^2$. 3. The hook splitting rate of the automatic line hauler is about $95.5\%$ regardless of hauling speed and materials of snood. 4. The case of unseparating hook is appeared when the snood gets entangled or the hook is sticked in the mainline.

• 보존과학연구
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• s.21
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• pp.5-55
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• 2000

The cultural properties are damaged by various causes according to the characteristics of material, the condition of preservation, and the period of time. Especially, biodeterioration makes lots of damages in organic properties than inorganic ones. The damages of wooden cultural properties by insects usually are caused by the three orders; Isoptera, Coleoptera, and Hymenoptera. As the result of investigation on the state of 141 buildings of wooden cultural properties in 1999, some of them were damaged by many kinds off actors; wasp, powder post beetle, cigarette beetle, termite, decay, and physical cracking. And it was found that the patterns of damages were related to species-specific habits of insects. There are several methods of pest control for the prevention of wooden cultural properties from damages caused by insects. Those are as follows; physical control, chemical control, biological control, and integrated pest management. When insects and fungi were detected at the wooden buildings, the fumigation is best treatment to stop biodeterioration. And then, wood materials also need to be treated with insecticidal and antiseptic chemicals to avoid a reinfestation, because the fumigant is volatile. The six commercial chemicals which are applied to the insecticidal and antiseptic treatment of wooden cultural properties were purchased to test their abilities. According to the comparative results of efficacy of them in laboratory, chemical D showed excellent efficacy in all items, including antiseptic and termiticidal items. The goal of these pest controls is to protect wooden buildings from insects and microorganisms. The most effective method used currently is chemical control(fumigation, insecticidal and anticeptic chemical treatment), but it has to be treated periodically to control pest effectively. Recently environmentally-friendly control methods such as bait system or biological treatments are replacing traditional barrier treatments using large amounts of chemicals. Especially, termite is a social insect which makes a colony. Although a building with fumigation treatment is safe for a while, once attacked building has a risk of damage by reinfestation of termite. Therefore, to control termites from damaged building, the entire colony including reproductives(queen and king) and larvae around buildings must beeliminated. Bait system can be used as a preventive measure in early detection of them through termites colony monitoring and baiting. It would be the most effective for termite control if bait system would be used together with the chemical controls.

• Korean journal of applied entomology
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• v.51 no.1
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• pp.67-72
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• 2012

This study was conducted to evaluate the scouting methods for larva and adults of the alfalfa weevil, $Hypera$ $postica$ Gyllenhal on Chinese milkvetch, $Astragalus$ $sinicus$ L. in the field. Three sampling methods, shake-bucket, shake-picking, and a sweeping net were evaluated for collecting alfalfa weevil larvae. We found significant differences among scouting methods and date in all survey fields. Sweep-net sampling was less able to detect small larvae than large larvae, which were not detected until early April whereas the shake-bucket, and shake-picking methods efficiently collected larvae from middle March. A Pitfall trap with three different baits - no bait, kidney bean seeds and sprouting kidney beans were compared for collection efficiency of alfalfa weevil adults. Collection efficiencies were significantly different by bait(p<0.018). Traps baited with sprouting kidney beans were the most efficient for collecting the alfalfa weevil. The number of alfalfa weevil caught was not significantly different between kidney bean seed and no bait in the pitfall trap. Therefore, the shake-bucket method for larvae and a pitfall trap baited with sprouting kidney beans for adults are recommended for scouting of the alfalfa weevil in a Chinese milkvetch field.

• Proceedings of the Korea Contents Association Conference
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• 2011.05a
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• pp.353-354
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• 2011

한국 학생들이 영어 모음들을 어떻게 인지하는지 알아보기 위해서, 49명의 한국 대학생들을 대상으로 11개의 영어 목표 모음 [i, ɪ, eɪ, ɛ, æ, ɑ, ɔ, oʊ, ʊ, u, ʌ]이 들어간 단어 beat, bit, bait, bet, bat, pot, bought, boat, put, boot, but들의 모음을 판별하는 인지실험을 실행하였다. 실험결과 bet, bought, boot의 모음을 판별하는 정확도가 50%이하로 낮았고, 반면에 bat, pot, boat의 모음 인지 정확도는 70% 이상으로 높았다. 영어 목표 모음별로 혼동도표를 통해서 목표 영어 모음들의 혼동패턴과 최소대립어 훈련도 제시되었다.

• Journal of applied mathematics & informatics
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• v.42 no.1
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• pp.65-76
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• 2024

In this paper, the I-transform of the Mellin convolution type is presented. Based on the Mellin transform theory, a general integral transform of the Mellin convolution type is introduced. The generating operators for I-transform together with the corresponding operational relations are also presented.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.15 no.1
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• pp.17-22
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• 1979

To catch live anchovies which will be used as bait for skipjack fishing, set net, lift net and purse seine net were tried in Korean coastal waters by the authors. Among them the former two methods showed unsuitable to catch in bulk even though the catch showed good survival. The last showed unsuitable result because of the lack of exclusive fishing boat and skilled fishermen. From the viewpoint of practical use, the authors regarded it may be suitable to improve the traditional boat seine net which are used as major fishing gear to catch anchovies in Korea, so far as Korea is concerned. So we tried to catch live anchovies with partially reconstructed boat seine net and to hold the catch in the creel improved by the authors. Even though the result showed less than 70 percent of survival, slightly worse than other fishing methods, the experimented method showed to be practical in catching the live anchovies intensively.

• BMB Reports
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• v.34 no.1
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• pp.15-20
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• 2001

One of the innate immune reactions in invertebrates is the pro-phenoloxidase (pro-PO) activation system that is involved in the generation of superoxide, melanin synthesis, and the subsequent sequestration of foreign matter entering the hemocoel of the invertebrates. However, the molecular mechanism of this biological reaction is still obscure. To expand our understanding of the biological roles of the pro-PO activation system in invertebrates, we performed a yeast two-hybrid screening by using three regions of pro-PO as bait and a yeast two-hybrid cDNA library from Tenebrio molitor larvae as prey We isolated a novel partial cDNA clone that encodes a glycine-rich protein that interacted with the active phenoloxidase (termed phenoloxidase interacting protein, POIP). POIP consists of two domains: One is an N-terminal unique domain and the other is a C-terminal glycine-rich domain. The C-terminal glycine-rich domain showed sequential homology with those of insect antifungal proteins. Also, the yeast two-hybrid screen in a reverse orientation (using POIP as bait) yielded PO, suggesting that the PO-POIP interaction is specific. By using a 315 bP PCR fragment of the N-terminal unique region of POIP, we cloned the full-length cDNA of POIP from the Tenebruo cDNA library constructed by using E. coli injected larvae. The interaction analysis between PO, and a truncated fragment lacking the N-terminal unique region of POIP, indicated that the N-terminal unique region is necessary for interaction between PO and POIP. The expression level of the POIP mRNA is increased by bacterial injection into T. molitor larvae. This suggests that POIP might be engaged in the humoral defense reaction.