• 대한미생물학회지
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• 제21권3호
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• pp.355-361
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• 1986

Vibrio vulnificus, a halophilic Vibrio has gained worldwide attention as a cause of severe and frequently fatal wound infections and life-threatening septicemia. For this reason V. vulnificus is thought to produce extreme hemoconcentration and rapid death after infection, and because V. vulnificus is thought to be less susceptible to bactericidal activity of normal human serum, the present study was undertaken to assess the susceptibility of V. vulnificus to human serum bactericidal activity with that of V. parahemolyticus and V. cholerae and to assess the effect of Vibrio species, Salmonella typhimurium and E. coli on hematocrit values in experimentally infected mice. Serum bactericidal activity against both V. vulnificus and V. cholerae was higher than against V. parahemoltyicus. Survival of the test strains in heat-inactivated human serum was greater than that in heat-uninactiveted serum. Both V. parahemolyticus and V. cholerae produced slight hemoconcentration within 2 to 6 hr after intraperitoneal injection of $10^7$ viable bacteria into mice. In contrast, V. vulnificus, S. typhimurium and E. coli produced hemodilution rather than hemoconcentration after 4 or 6 hr after infection. With these results the author can conclude that V. vulnificus is more susceptible to serum bactericidal activity than other Vibrio species, and V. vulnificus did not produce hemoconcentration.

• 미생물학회지
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• 제38권4호
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• pp.281-286
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• 2002

2000년 1 월부터 2000년 10월까지 가두리 양식장에서 Vibrio vulnificus를 검출하였고 이들의 억제 방법을 연구하였다. 이 세균의 검출은 선택적 분리법과 vvhA 유전자를 확인하는 방법을 이용하였다. V. vulnificus는 수온이 $17^{\circ}C$이상인 5월부터 검출되었고 $19^{\circ}C$ 이상인 6월부터 9월까지는 대부분의 시료에서 검출되었다. V. vulnificus를 제어하기 위한 방법 중 냉동 및 냉장 처리는 살균효과를 나타내지 못했다. Citric acid도 균의 생장을 억제하지 못했으나, 500 mg/1이상의 EDTA가 첨가될 경우 균이 완전히 사멸되었다. 분말 광촉매인 산화티타니움은 자외선을 조사할 경우 15분～1 시간 이내에 이 세균을 완전히 사멸시키는 효과를 나타내었다. 산화티타니움을 유리 구슬에 코팅한 광촉매 장치를 이용하여 0.2/min의 turnover rate로 사멸효과를 얻었다.

• 한국식품과학회지
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• 제31권2호
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• pp.522-526
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• 1999

S. mutans에 대한 키토산의 항균효과는 그 분자량에 따라 뚜렷한 차이를 보였으며, 약 30,000의 분자량을 갖는 키토산이 가장 높은 항균활성을 보였다. 키토산 처리에 의해 세포로부터 단백질, 핵산, 2가 양이온 등이 상당량 유출되는 것이 관찰되었으며, 세포내 물질의 유출정도는 키토산의 항균효과와 밀접한 상관성을 보였다. 이러한 세포내 물질의 유출은 양이온성을 띠는 키토산의 아민기와 세포벽내의 음이온 성분과의 강한 결합에 의해 세포벽의 결합력이 약화되면서 생기는 결과로서 추측되었다. 키토산처리에 의해 S. mutans의 세포벽이 원형질로부터 분리되고 세포내 물질이 세포벽 내에서 밖으로 유출되는 현상은 투과전자현미경(TEM)에 의해 확인되었다.

• 한국축산식품학회지
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• 제43권3호
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• pp.402-411
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• 2023

This study was conducted to investigate the bactericidal effect of nisin (Nisin) only, atmospheric pressure plasma (APP) only, and a combination of APP and nisin (APP+Nisin)(APP+Nisin) on beef jerky and sliced ham inoculated with Escherichia coli O157:H7, gram-negative bacteria. The bactericidal effect against E. coli O157:H7 and Listeria monocytogenes was confirmed using a nisin solution at a concentration of 0-100 ppm, and APP+Nisin was tested on beef jerky and sliced ham using 100 ppm nisin. Beef jerky and sliced ham were treated with APP for 5 min and 9 min, respectively. In the bacterial solution, 100 ppm nisin out of 0-100 ppm nisin exhibited the highest bactericidal activity against L. monocytogenes (gram-positive bacteria; p<0.05); however, it did not exhibit bactericidal effects against E. coli O157:H7 (gram-negative bacteria). The APP+Nisin APP+Nisin exhibited a 100% reduction rate in both E. coli O157:H7 and L. monocytogenes compared to the control group, and was more effective than the Nisin. The APP+Nisin decreased the number of colonies formed by 0.80 and 1.96 Log CFU/g for beef jerky and sliced ham, respectively, compared to the control, and exhibited a higher bactericidal effect compared to the Nisin (p<0.05). These results demonstrate the synergistic bactericidal effect of APP and nisin, providing a possible method to improve the limitations of nisin against gram-negative bacteria. In addition, this technology has the potential to be applied to various meats and meat products to control surface microorganisms.

• 한국임상수의학회지
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• 제27권3호
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• pp.252-256
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• 2010

소 유방염은 주로 세균 감염에 의한 유선조직에 염증이 생기는 질병으로 유방염의 치료와 억제를 위해서는 1차적으로 세균을 사멸시키는데 있다. 본 연구에서는 소 유방염의 원인이 되는 다양한 세균들에 대한 은 이온의 항균효과와 적용 농도를 측정하였다. 유방염 원인균에 대한 은 이온의 최소 억제 농도 (minimal inhibitory concentrations, MICs)와 최소 살균 농도 (minimum bactericidal concentrations, MBCs)를 측정하였으며, 주사전자현미경 (scanning electron microscopy, SEM)을 이용하여 은 이온에 의한 세균의 형태 변화를 확인하였다. 다양한 유방염 원인균에 대한은 이온의 최소 억제 농도와 최소 살균 농도는 1.9에서 15.6 ${\mu}g$/ml로 세균에 따라 차이가 보였으며, 은이온 처리 후 주사전자현미경 촬영 결과 Staphylococcus aureus와 Escherichia coli의 세포벽이 함몰되거나 파괴되는 것을 볼 수 있었다. 이러한 결과는 유방염 원인이 되는 다양한 원인균에 대해 은이온의 살균 효과와 그 적용 농도를 보여주는 것으로, 소 유방염의 치료와 방어를 위한 임상적인 적용 가능성을 보여주는 것이다.

• 한국세라믹학회지
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• 제34권1호
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• pp.13-22
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• 1997

은 이온과 구리 이온을 동시에 함유하는 살균용 인산염계 수용성 유리를 용융법으로 제조하였다. 분말상의 유리 시편을 상온의 증류수에 넣어 조성과 시간에 따른 용해 특성을, bulk상 시편에 대해서는 용해 전.후의 표면변화를 각각 관찰하였고, 살균 효과 및 세포독성을 평가하였다. 은 이온:구리 이온=1:1인 조성에서 최대 용해량을 나타내었으며 용해거동은 초기부터 total dissolution이 지배적이었다. 시간의 경과에 따라 용해는 진행이 되지만, 표면에 생성되는 새로운 층은 관찰되지 않았다. Pseudomonas sp, e. coli, staphylococcus aureus, 및 salmonella균에 대한 살균 효과는 용해량의 증가에 따라 증가하여 우수한 살균효과를 나타내었다. L929 세포에 대한 독성 실험 결과, 은 이온의 농도가 10ppm이상에서만 독성을 나타내었다.

• 한국세라믹학회지
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• 제33권2호
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• pp.242-250
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• 1996

Glasses of Na2O-Al2O3-SiO2 system were prepared and ion-exchange characteristics change of properties and bactericidal effects by Na+↔Ag+ ion exchange were studied. Parent glasses with three compositions of varying Na2O in the 20~30 wt% were ion-exchanged in the molten salt of 2 mol% AgNO3+98 mol% NaNO3 at 320~36$0^{\circ}C$ for 15~16min. Amount of ion exchange and penetration depth of Ag+ ion increased with Na2O content in the parent glass ion exchange temperature and time. After ion exchange densities and Vickers hardness of the glasses increased and the glasses showed yellow-brown color and as amount of ion exchange increased the color turned deep because partial reduction and agglomeration of Ag+ into Ag0 results in absorption of visible light. After ion exchange chemical durability of the glass to wter was enhanced compara-bly that weight loss and change of surface of the glass were not found for the leaching test in 5$0^{\circ}C$ K.I water for 240hrs. Bactericidal effect of ion exchanged glass on Staphylococcus aureus and E. coli was determined by microorganism test and bactericidal effect increased with amount of ion exchange and incubation time.

• Restorative Dentistry and Endodontics
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• 제1권1호
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• pp.43-49
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• 1975

This study was divided into two parts. In the first experiment, the in vitro antimicrobial effect was tested in order to evaluate the effect of vapors, and the effectiveness of the nonspecific endodontic medicaments (formocresol, camphorated parachlorophenol and eugenol). In the second experiment, the intracanal effect was tested in vitro under simulated clinical condition. The actual bactericidal effect of the nonspecific endodontic medicaments (formocresol, camphorated parachlorophenol and eugenol) was quantitated. The results were obtained as follows: 1. The zone of inhibition was appeared on the vapors of formocresol only, however there were no zone of inhibition appeared on the vapors of camphorated parachlorophenol and eugenol. 2. Formocresol produced the widest zone of inhibition and eugenol, the next and camphorated parachlorophenol, the narrowest. 3. All of the tested medicaments were vaporized in the root canal. They proved to be the effective antimicrobial activity in the root canal. 4. All of the tested medicaments were showed more bactericidal effect at 72 hours than 48 hours. 5. In comparing with the bactericidal effect of the tested medicaments in the root canal, formocresol was showed the most bactericidal medicament, camphorated parachlorophenol was showed the least. 6. Complete sterilization of the root canal was not achieved in any medicaments applied in this study.

• International Journal of Oral Biology
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• 제43권3호
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• pp.129-132
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• 2018

Enterococcus faecalis is a major causative agent of endodontic treatment failure. The purpose of this study was to investigate bactericidal effects of ethanol extract of Garcinia mangostana L. (mangosteen extract) on five strains of E. faecalis that were isolated from human oral cavities. The bactericidal effects of mangosteen extract were assessed by measurement of minimum bactericidal concentration (MBC) value. The cytotoxicity of mangosteen extract on immortalized human gingival fibroblasts, hTERT-hNOF, was determined based on cell counting method. The data revealed the MBC value of mangosteen extract against the E. faecalis strains was $4{\mu}g/ml$. Additionally, the cell viability of mangosteen extract on hTERT-hNOF was 83.7-89.1% at the 1 to $16{\mu}g/ml$. These findings indicated that mangosteen extract could be used as a root canal cleaner during management of endodontic treatment failure caused by E. faecalis.

• International Journal of Oral Biology
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• 제43권3호
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• pp.123-127
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• 2018

Polyphenon 60 refers to the mixture of catechins present in green tea. The aim of this study was to investigate the antimicrobial activities of polyphenon 60 against 4 strains of Streptococcus mutans and 2 strains of Streptococcus sorbrinus, which are the major causative bacteria of dental caries. The minimum bactericidal concentration (MBC) values of polyphenon 60 for S. mutans and S. sobrinus were determined and the effect of biofilm formation inhibition of that was evaluated. The MBC value of polyphenon 60 against the bacterial strains was 2.5 mg/ml except for one particular strain, S. mutans KCOM 1128 for which the value was 1.25 mg/ml. The results of biofilm formation inhibition assay revealed that polyphenon 60 inhibited biofilm formation more than 90% at a concentration of 2.5 mg/ml. It was apparent that polyphenon exhibited biofilm formation inhibition activity along with bactericidal effect against S. mutans and S. sobrinus. Therefore, it is proposed that polyphenon 60 as one of the components of bactericidal agents could be useful in developing oral hygiene products, toothpaste or gargling solution.