• Korean Journal of Veterinary Service
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• v.15 no.2
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• pp.150-165
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• 1992

The study for a effect of monitoring on bovine mastitis was conduced for a year from Dec, 1989 to Nov, 1990, Sampling the bulk milk of 350 herds in Inchon city and out of 10 herds among them were carried out herds guidance, CMT, SCC, isolation of pathogens and antibiotic sensitivity tests. The results obtained were summarized as follows 1. Annual mean SCC of 1213 herds was 558, 000 cell /ml 2. The number of SCC below 500, 000 cell /ml to quarters for herds guidance was at 1st 77. 0%, End 84.8% and 3rd 80.4%. The is shown that milk quality was steadly improved. 3. The most number of isolated pathogens of bovine mastitis was Staphylococcus SPP - 402(47.2%) Streptococcus SPP - 80(18.7% ) 4. The highest rate of antibiotic sensitivity test was Stapylococcus SPP - cephalothin(76.7%) Streptococcus SPP - ampicillin(77.5%) Gram negative bacilli - tetracyclin(76.0%) 5. The effect of monitoring on bovine mastitis was improved showing that at 1st 49.0% to 3rd 72.0%

• Journal of Chest Surgery
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• v.11 no.1
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• pp.12-17
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• 1978

Rifampicin has been widely hailed as the most effective antituberculosis antibiotics since the clinical use of streptomycin, but its immunosuppressive side effect was still annoying problem to be excluded. These studies were carried out to determine the effect of Tuberein-3, tuberculous bacilli extraction with water, on Rifampicin induced T-lymphocytopenia in 5 cases of pulmonary tuberculosis who have never exposed to antimetabolites or steroid compounds. After 2 weeks treatment of Rifampicin, all cases showed T-lymphocytopenia, active $13.0{\pm}2.3$ % and total $43.1{\pm}4.4$%. Followed by another 2 weeks treatment with Rifampicin combined with Tuberein-3, T-lymphocytes in peripheral blood returned to the normal limit, active $21.6{\pm}3.3$% and total $56.3{\pm}1.7$%. Tubercin-3 revealed the restoring activity of suppression of T-lymphocyte rosettes by Rifampicin.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.309-312
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• 2000

A halophilic bacterium was isolated from domestic marine. The bacterium was gram negative and motile. Transmission electron micrograph after cultivating for 6-, 20-, 72-, and 144 hrs showed that it was bacilli and contained intracellular granules, which were pleomorphic, larger in density by the time and considered to be PHB since they were positive on the sudan black B staining. The presence of sodium chloride was critical, because the isolated marine bacterium could not multiply and even produce any immunostimulant in the deficiency of sodium chloride. The strain produced an immunostimulant, which was investigated for the biological characteristics. The optimal conditions for the production of the immunostimulant were 1 % dextrose and 1 % yeast extract in artificial sea water for carbon and nitrogen sources, respectively. The initial pH and growth temperature for the production were 8.0 and $30^{\circ}C$ under the presence of oxigen, respectively.

• Tuberculosis and Respiratory Diseases
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• v.79 no.1
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• pp.42-45
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• 2016

Raoultella species are gram-negative, non-motile, aerobic bacilli that are primarily considered as environmental bacteria. Raoultella planticola is reportedly a rare cause of human infections. Also, the definite pathological mechanism of Raoultella planticola is currently unknown. We report a case of pneumonia caused by Raoultella planticola.

• The Korean Journal of Cytopathology
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• v.4 no.2
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• pp.100-104
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• 1993

Sputum smears of 116 cases in 55 pulmonary tuberculosis patients with the demonstration of acid-fast bacilli in sputum were retrospectively reviewed to investigate the cytologic diagnosis of pulmonary tuberculosis in sputum. Epithelioid cells, multinucleated giant cells of Langhans or nonspecific type, or necrotic materials of caseation or nonspecific nature were found in 40% of the cases, but in 60% of the cases, only nonspecific findings including squamous metaplasia were found. Only in 6% of the cases, the cytologic diagnosis of tuberculosis was possible with the findings of epithelioid cells and muitlnucleated giant cells in a necrotic background.

• Journal of Microbiology and Biotechnology
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• v.17 no.4
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• pp.668-672
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• 2007

A microcosmal experiment using a metagenomic technique was designed to assess the effect of BTEX (benzene, toluene, ethylbenzene, and xylenes) on an indigenous bacterial community in a Daejeon forest soil. A compositional shift of bacterial groups in an artificial BTEX-contaminated soil was examined by the 16S rDNA PCR-DGGE method. Phylogenetic analysis of 16S rDNAs in the dominant DGGE bands showed that the number of Actinobacteria and Bacillus populations increased. To confirm these observations, we performed PCR to amplify the 23S rDNA and 16S rDNA against the sample metagenome using Actinobacteria-targeting and Bacilli-specific primer sets, respectively. The result further confirmed that a bacterial community containing Actinobacteria and Bacillus was affected by BTEX.

• Journal of Korean Foot and Ankle Society
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• v.4 no.1
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• pp.30-34
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• 2000

The Actinomyces organism is inherent in human as normal flora found in the mouth. Outside of the mouth, this organism can cause actinomycosis. Involvement of the extremity is very rare and atypical. The authors have experienced a case of actinomycosis of the foot and report with the literature review. Histopathologic finding shows multiple sulfur granules. In histochemical stains, periodic acid-Schiff, Gomori methionine silver, and gram stain were positive, and acid fast bacilli stain was negative. The patient was treated with surgical excision and antibiotic administration with Ampicillin for 6 months.

• Journal of Chest Surgery
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• v.13 no.1
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• pp.48-51
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• 1980

Open heart surgery patients have additional risks arising from the special nature of the operative procedure. And postoperative infections in patients with extracorporeal circulation are associated with high incidence of serious sequelae. To investigate the incidence and organisms of contamination, and the relation between the duration of extracorporeal circulation and contamination, following study was done. Eighty-four of the open heart surgery patients were examined with cultures from the blood and priming solution before and after bypass. 1. Cultures before bypass were sterile, but 2 cases of cultures from the blood and priming solution after bypass were positive, and culture positive group was 5% of all patients. 2. The organisms were Gram-negative, aerobic coliform bacilli; 3. The culture positive group had significantly longer bypass time.

• Korean Journal of Veterinary Research
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• v.1 no.1
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• pp.72.2-72.2
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• 1961

Since April, 1957, 200 White Leghorn and 100 Newhampshire had been brought to the poultry farm of our college from Songwhan National Breeding Station. The looses due to avian tuberculosis were 67 chickens by June, 1959. Thirteen of them were examined histopathologically. Grayish white tuberculous nodules varying in size were recognized in the liver, spleen, bone marrow, and intestine of all affected birds. The heart rind testis were involved in each case, and pulmonary tuberculosis which, unlike in mammals, was kown to be rarely encountered in birds was observed in two cases. One case showed amyloid like degeneration in hepatic cell cords of the liver, glomerular tufts and collecting ducts of the kidney. Lesions in the spleen presented typical Sago spleen. Sections from the liver, spleen, intestine, and lungs were stained by Ziel-Neelsen stain. Acid Fast stained bacilli were found embeded in the cytoplasm of epithlioid cells and foreign-body giant cells.

• Korean Journal of Veterinary Service
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• v.29 no.4
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• pp.497-501
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• 2006

Acute hemorrhagic enteritis was diagnosed in a seven-month-old male Shitzu dog dying of blood stained diarrhea and vomiting. Clinical findings were anorexia, dullness and sudden death after massive bloody diarrhea. At necropsy, main lesion was the hemorrhage in small intestine, mainly duodenum and jejunum. Microscopically, Gram positive long bacilli were massively detected on the mucose epithelial cells and necrotic debris of small intestine. Coagulative necrosis of epithelial cells and thrombosis of small intestine were also identified. However, there was no lesion of crypt epithelium. Mineral infiltration in both gastric mucosa and renal tubules was detected and proliferation of fibrous tissue was also shown in corticomedullary regions. In bacterial examination, C perfringens was isolated in anaerobic culture and it was confirmed to type A by multiplex PCR. Therefore, the dog was diagnosed as C perfringens type A associated enteritis with uremia.