• Journal of Korean Elementary Science Education
• /
• v.27 no.3
• /
• pp.244-251
• /
• 2008

One of the problems with testing for the presence of $CO_2$ is that the results are not visible. In order to over-come this weak point, a new testing method was developed with BTB indicator equipment, that made the gas visible. After that, the experiment was performed and tested the effects of the new visible equipment. The visible equipment could be adapted to regular class use successfully. Also, it was effective in that it reduced waste of gas, minimized danger potential through use of $CO_2$ canisters and candle-sticks, and also increased knowledge about indicators. The new experimental method and equipment affected the students' interest. It is possible that the students' positive participation was due to their interest in the new apparatus and application of the visual senses. The new system was tested fer its effects on teaching content and helping to produce sustained memory of the content. There were no significant differences between the groups in terms of content learning on initial content memory. However, when students in both groups were tested 4 months later, the visual experiment group sustained memory performance, while the other group showed a significant decrease. Generally, boys score higher than girls in terms of interest and participation in experimental activities. In this case, however, there were no difference between groups. It may have been due to introduction of new equipment and different methods from the textbooks. So, this could increase participation in science using various experiments.

• Asian-Australasian Journal of Animal Sciences
• /
• v.33 no.3
• /
• pp.398-407
• /
• 2020

Objective: The Yip1 domain family (YIPF) proteins were proposed to function in endoplasmic reticulum (ER) to Golgi transport and maintenance of the morphology of the Golgi, which were homologues of yeast Yip1p and Yif1p. YIPF3, the member 3 of YIPF family was a homolog of Yif1p. The aim of present study was to investigate the expression and regulation mechanism of porcine YIPF3. Methods: Quantitative realtime polymerase chain reaction (qPCR) was used to analyze porcine YIPF3 mRNA expression pattern in different tissues and pig kidney epithelial (PK15) cells stimulated by polyinosine-polycytidylic acid (poly [I:C]). Site-directed mutations combined with dual luciferase reporter assays and electrophoretic mobility shift assay (EMSA) were employed to reveal transcription regulation mechanism of porcine YIPF3. Results: Results showed that the mRNA of porcine YIPF3 (pYIPF3) was widely expressed with the highest levels in lymph and lung followed by spleen and liver, while weak in heart and skeletal muscle. Subcellular localization results indicated that it expressed in Golgi apparatus and plasma membranes. Upon stimulation with poly (I:C), the level of this gene was dramatically up-regulated in a time- and concentration-dependent manner. pYIPF3 core promoter region harbored three cis-acting elements which were bound by ETS proto-oncogene 2 (ETS2), zinc finger and BTB domain containing 4 (ZBTB4), and zinc finger and BTB domain containing 14 (ZBTB14), respectively. In which, ETS2 and ZBTB4 both promoted pYIPF3 transcription activity while ZBTB14 inhibited it, and these three transcription factors all played important regulation roles in tumorigenesis and apoptosis. Conclusion: The pYIPF3 mRNA expression was regulated by ETS2, ZBTB4, and ZBTB14, and its higher expression in immune organs might contribute to enhancing ER to Golgi transport of proteins, thus adapting to the immune response.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.46 no.5
• /
• pp.600-607
• /
• 2017

The total acidity (TA) of Makgeolli was affected by suspended solids and $CO_2$ produced during the fermentation process. Nine Makgeollis (four sterilized and five unsterilized Makgeollis) were collected in the market, and their TAs were compared before and after filtration and $CO_2$ removal. TAs of sterilized Makgeollis were 0.379~0.477%, which significantly decreased to 0.167~0.225% after filtration and 0.132~0.170% after $CO_2$ removal (P<0.05). TAs of unsterilized Makgeollis were 0.412~0.467% and decreased to 0.157~0.365% after filtration and 0.143~0.280% after $CO_2$ removal (P<0.05). TAs of Makgeollis were compared by three methods using different indicators. The TAs of sterilized and unsterilized Makgeollis were 0.105~0.123% and 0.105~0.200%, respectively, by bromthymol blue+neutral red (light green), 0.129~0.154% and 0.130~0.255%, respectively, by phenolphthalein (faint pink), and 0.120~0.146% and 0.130~0.232%, respectively, by bromthymol (blue). Nowadays, Makgeolli is commercialized with various distinct colors, and thus it is important to select appropriate indicators for proper titration endpoint identification for TA measurement. The compositions of organic acids profiles varied depending on sterilized or unsterilized Makgeollis, in which oxalic acid (0.108~0.329 mg/mL), malic acid (ND to 0.134 mg/mL), lactic acid (0.127~0.776 mg/mL), and citric acid (ND to 1.159 mg/mL) were found, and lactic acid was in unsterilized more than sterilized Makgeollis.

• Progress in Superconductivity and Cryogenics
• /
• v.5 no.2
• /
• pp.1-7
• /
• 2003

Nowadays, one of the serious problems in KEPCO(Korea Electric Power Co-Operation) system is the more higher fault current than the SCC(Short Circuit Capacity) of circuit breaker. There are many alternatives to reduce the increased fault current such as isolations of bus ties, enhancement of SCC of circuit breaker, applications of HVDC-BTB(High Voltage Direct Current-Back to Back) and FCL(fault current limiter). But, these alternatives have some drawbacks in viewpoints of system stability and cost. As the superconductivity technology has been developed, the HTS-FCL(High Temperature Superconductor -Fault Current Limiter) can be one of the attractive alternatives to solve the fault current problem. Under this background, this paper presents the EMTDC(Electro-Magnetic Transient Direct Current) model for resistance type HTS-FCL considering the nonlinear characteristic of final resistance value when quenching phenomena occur.