• The Plant Pathology Journal
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• v.26 no.4
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• pp.306-312
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• 2010

The pathotypes of Cercospora beticola, causal agent of sugar beet leaf spot disease, were identified by application of pathogenicity test using 100 isolates obtained from the provinces with intensive sugar beet cultivation. For the identification of pathotypes, five sugar beet cultivars were used each with different resistance factors. Cultivar reactions were determined by inoculation of cultivars with the isolates under controlled conditions and measuring disease severity on the $15^{th}$ day according to the 1-9 KWS Scale. Based on the reactions of the five cultivars, a total of 15 pathotypes were detected. All employed sugar beet cultivars were resistant to Pathotype no:1 comprising most of the isolates. Genetic diversity of the causal agent was characterized by AFLP reaction. The products acquired at the end of AFLP reaction were detected by means of Beckman CEQ 8800 DNA Capillary Series Analysis and the results obtained were evaluated according to the similarity index UPGMA. For the genetic analysis of C. beticola isolates, 9874 polymorphic fragments of sizes between 100 and 500 bp were analysed which were generated by nine primers. The dendrogram derived from AFLP analysis depicted the existence of five different subgroups. The polymorphism rate among isolates was 91.13% and the dendrogram distribution of the pathotypes obtained by pathogenicity indicated that pathotypes were not discriminated and did not compose any groups.

• International Journal of Industrial Entomology and Biomaterials
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• v.36 no.2
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• pp.25-30
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• 2018

The larvae of Hermetia. illucens have a high probability of coming into contact with microorganisms such as bacteria and fungi. Therefore, the survival of H. illucens is primarily the protection of their own against microbial infection. This effect depends on the development of the innate immune system. Antimicrobial Peptides (AMPs) exhibit antimicrobial activity against other bacterial strains and can provide important data to understand the basis of the innate immunity of H. illucens. In this study, we injected larvae with Enterococcus. faecalis (gram-positive bacteria) and Serratia. marcescens as (gram-negative bacteria) to test the hypothesis that H. illucens is protected from infection by its immune-related gene expression repertoire. To identify the inducible immune-related genes, we performed and cataloged the transcriptomes by RNA-Seq analysis. We compared the transcriptomes of whole larvae and obtained a DNA fragment of 465 bp including the poly (A) tail by RACE as a novel H. illucens immune-related gene against bacteria. A novel target mRNA expression was higher in immunized larvae with E. faecalis and S. marcescens groups than non-immunized group. We expect our study to provide evidence that the global RNA-Seq approach allowed for the identification of a gene of interest which was further analyzed by quantitative RT-PCR, together with genes chosen from the available literature.

• The Plant Pathology Journal
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• v.21 no.4
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• pp.328-333
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• 2005

Paenibacillus elgii SD17 (KCTC $10016BP^T$=NBRC $100335^T$) was recently reported as a new species. Based on its inhibitory activity to Thanatephorus cucumeris AG1-1, strain SD17 was further evaluated for its potential as a biocontrol agent against soil-borne diseases of turf grasses in Korea. P. elgii SD17 showed a broad spectrum of antimicrobial activity in vitro test and suppressed development of turf grass diseases; Pythium blight caused by Pythium aphanidermatum and brown patch caused by T. cucumeris AG1-1 on creeping bentgrass (Agrostis palustris) in the growth chamber tests. Under a condition for massive culture in a 5,000 L fermenter, P. elgii SD17 reached $6.4{\times}10^8$ spores/ml that resulted in approximately $1.0{\times}10^7$ cfu/g when formulated into a granule formulation (GR) using the whole culture broth instead of water. Using the GR formulation, biocontrol activity of P. elgii SD17 was confirmed. In the growth chamber tests, the GR formulation was effective against brown patch and Pythium blight with similar level of disease severity compared to each of the standard fungicides at the application rates of 10 g/$m^2$ or above. In the field tests, compared to each untreated control, the GR formulation also effectively controlled Pythium blight, brown patch and large patch at all the application rates of 5, 10 and 20 g/$m^2$, respectively, without significant response by the application rates. However its performance was inferior to each of the standard chemical fungicides. Based on these results, we consider this GR formulation of P. elgii SD17 as an effective biocontol agent to suppress Pythium blight, brown patch and large patch of turf grasses in Korea.

• Journal of Life Science
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• v.22 no.3
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• pp.285-292
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• 2012

Genus Spiraea is composed of many long-lived woody species that are primarily distributed throughout Asia and Europe. In this study, we evaluated a representative sample of the 38 taxa in the world, including 14 in Korea, with nuclear ribosomal DNA internal transcribed spacer sequences (ITS) to estimate genetic relationships within the genus. The molecular data allowed us to resolve well-supported clades in the taxa. In 47 world accessions (38 taxa: 14 Korean taxa, 33 world taxa, and 9 overlapping taxa), total alignment length was 689 positions, of which 452 were parsimony informative, 527 variable, 75 singleton, and 159 constant characters. Although the phylogenic tree showed that many taxa of genus Spiraea were well separated from each other, many branches were not congruent with the morphological characteristics and geographical distributions of the genus. There were 430 segregating sites and the nucleotide diversity (${\pi}$) value was 0.281. Under the neutral mutation hypothesis, the probability that the Tajima test statistic (D) is positive (2.325) is more than 0.5. Therefore, there may be a site at which natural selection, which increases genetic variation, is operating.

• Journal of The Geomorphological Association of Korea
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• v.20 no.3
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• pp.55-63
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• 2013

The study deals with the geochemical analyses on the stratigraphy and sedimentation of the paleofluvial outcrop under Jeongok basalt layer in Chugaryeong Rift Valley. The results of field survey and analysis show that the paleofluvial sand and gravel layers had been constructed before the arrival of basaltic lava in Jeongok area of Hantan River valley in central Korea. XRF, for the main elements analysis, results present that JG2-3, a test sample from basalt weathering layer, has different characteristics from 5 other samples in the aspects of chemical weathering levels. ICP-MS, for analysis of the rare earth elements analysis, estimates that gravel layer, in the lowest part of the outcrop, had been originated from pre-lava bedrocks near the upper reach of the Hantan River, so the layer is almost not related to basalt layers. Finally, the OSL dating results in paleofluvial sedimentation had been in process until nearly 40,000 BP, before formation of lava plateau in the Jeongok area.

• The Journal of Korean Obstetrics and Gynecology
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• v.27 no.4
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• pp.15-24
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• 2014

Objectives: There were only small studies about the difference of the quality of life depending on blood stasis. We investigated that the blood stasis made the differences of quality of life. Methods: We recruited the participants stratified according to age and gender. The participants were assessed independently by two Korean medicine doctors more than 7 years of clinical experiences whether or not with blood stasis. And they responded at the questionnaire associated with blood stasis and SF-36. The analysis was done with IBM SPSS Statistics (ver. 21). Results: Total 101 participants were checked for with/without blood stasis. 40 participants were accord in the diagnosis with blood stasis by two doctors. Two researchers coincided in diagnosis with non-blood stasis in 42 participants. 19 participants showed the discrepancy between the assessment of blood stasis and they were classified as non-blood stasis. The mean age in group of blood stasis was $44.07{\pm}11.65$, it was not shown the significant difference comparing non-blood stasis group. The subscale of physical function (PF), role-physical (RP), bodily pain (BP), general health (GH), vitality (VT), mental health (MH) and role emotional (RE) in SF-36 were significantly low in the group of blood stasis. Blood test including biochemistry, fibrinogen, prothrombin time and D-dimer was not in abnormal range according to blood stasis without CRP. Conclusions: The patients that diagnosed of blood stasis showed the bad physical health status, and partially bad mental health. It is necessary to investigate whether Korean traditional treatment for blood stasis would be made improvement for quality of life. Moreover, the standardization of diagnosis on blood stasis would be needed.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.54 no.5
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• pp.617-623
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• 2021

The study was conducted to investigate the dietary supplementation of citrus by-product (CBP) and CBP fermented with Bacillus subtilis (BS) or B. pumilus (BP) on growth performance, feed utilization, innate immune responses and disease resistance of Korean rockfish Sebastes schlegelii. Triplicate groups of juvenile fish (initial weight of 22.9 g) were fed one of five experimental diets which were formulated to replace a synthetic vitamin C (L-ascorbyl-2-polyphosphate, LAPP) with CBP or fermented CBP. A basal diet without LAPP and CBP was used as the control diet (Con). At the end of the 13 weeks feeding trial, growth performance, feed utilization and survival of fish fed the Con were significantly lower than those of fish fed the LAPP, CBP and fermented CBP diets. Fish fed the Con were significantly lower in lysozyme activity than fish fed LAPP, CBP and fermented CBP diets. In a challenge test against Edwardsiella tarda, cumulative mortality of fish fed LAPP, CBP and fermented CBP diets was significantly lower (20-30%) than that of the Con (60%). The findings in this study indicate that the CBP and/or fermented CBP can be a promising vitamin C source for LAPP replacement in diet for Korean rockfish.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.7
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• pp.939-948
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• 2019

Objective: Extension and Agouti loci play a key role for proportions of eumelanin and pheomelanin in determining coat color in several species, including goat. Mongolian goats exhibit diverse types of coat color phenotypes. In this study, investigation of the melanocortin 1 receptor (MC1R) coding region in different coat colors in Mongolian goats was performed to ascertain the presence of the extension allele. Methods: A total of 105 goat samples representing three goat breeds were collected for this study from middle Mongolia. A 938 base pair (bp) long coding region of the MC1R gene was sequenced for three different breeds with different coat colors (Gobi Gurwan Saikhan: complete black, Zalaa Jinstiin Tsagaan: complete white, Mongolian native goat: admixture of different of coat colors). The genotypes of these goats were obtained from analyzing and comparing the sequencing results. Results: A total of seven haplotypes defined by five substitution were identified. The five single nucleotide polymorphisms included two synonymous mutations (c.183C>T and c.489G>A) and three missense (non-synonymous) mutations (c.676A>G, c.748T>G, and c.770T>A). Comparison of genotypes frequencies of two common missense mutions using chi-sqaure ($x^2$) test revealed significant differences between coat color groups (p<0.001). A logistic regression analysis additionally suggested highly significant association between genotypes and variation of black versus white uniform combination. Alternatively, most investigated goats (60.4%) belonged to H2 (TGAGT) haplotype. Conclusion: According to the findings obtained in this study on the investigated coat colors, mutations in MC1R gene may have the crucial role for determining eumelanin and pheomelanin phenotypes. Due to the complication of coat color phenotype, more detailed investigation needed.

• Tuberculosis and Respiratory Diseases
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• v.82 no.2
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• pp.126-132
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• 2019

Background: The development of lung cancer results from the interaction between genetic mutations and dynamic epigenetic alterations, although the exact mechanisms are not completely understood. Changes in DNA methylation may be a promising biomarker for early detection and prognosis of lung cancer. We evaluated the serial changes in genome-wide DNA methylation patterns in blood samples of lung cancer patients. Methods: Blood samples were obtained for three consecutive years from three patients (2 years before, 1 year before, and after lung cancer detection) and from three control subjects (without lung cancer). We used the MethylationEPIC BeadChip method, which covers the 850,000 bp cytosine-phosphate-guanine (CpG) site, to conduct an epigenome-wide analysis. Significant differentially methylated regions (DMRs) were identified using p-values <0.05 in a correlation test identifying serial methylation changes and serial increase or decrease in ${\beta}$ value above 0.1 for three consecutive years. Results: We found three significant CpG sites with differentially methylated ${\beta}$ values and 7,105 CpG sites with significant correlation from control patients without lung cancer. However, there were no significant DMRs. In contrast, we found 11 significant CpG sites with differentially methylated ${\beta}$ values and 10,562 CpG sites with significant correlation from patients with lung cancer. There were two significant DMRs: cg21126229 (RNF212) and cg27098574 (BCAR1). Conclusion: This study revealed DNA methylation changes that might be implicated in lung cancer development. The DNA methylation changes may be the possible candidate target regions for the early detection and prevention of lung cancer.

• Journal of The Geomorphological Association of Korea
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• v.25 no.4
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• pp.63-75
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• 2018

The lower marine terrace 1 and 2 surfaces distributed between Ulsan and Pohang coast in the southeastern coast of the Korean penninsula have been correlated with MIS 5e and 5a (or 5c) by amino acid dates, 14C dates, wide-spread tephra correlation and pollen analysis respectively. In this study, to test the reliability of the OSL method for the estimation of the numerical burial age of marine sediment deposits, we analyzed the samples from the marine terraces which have been known as typical marine terraces formed during MIS 5e and MIS 5a in the above-mentioned coast. The burial ages of the marine deposit of the lower marine terrace 1 and 2, with paleoshoreline altitudes of 18m to 19m and 10m to 11m respectively, both showed about the same age of 60 ka BP. The lower marine terraces 1 and 2, however, were divided into two terrace surfaces by a clear terrace cliff. Besides, the OSL dates of the lower and upper parts of the lower marine terrace 2 of the Bonggil coast showed the reversed burial ages. In the lower marine terrace 1 of the Sanhari coast, almost the same burial ages were derived from both the lower part (marine rounded gravel layer) and the upper part (terrestrial angular gravel layer) of the terrace deposit. Therefore, at the present time, judging from only the OSL dates measured in this study, it could be argued that the OSL method is not the best for the estimation of forming periods of the lower marine terraces 1 and 2 and their classification.