• 미생물학회지
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• 제41권1호
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• pp.13-17
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• 2005

본 연구에서는 이 전 연구에서 단일 탄소원과 질소원 및 에너지원으로 aniline을 이용하는 Delftia sp. JK-2에서 분리, 정제된 바 있는 C2,3O의 N-말단 아미노산과 DNA 서열을 분석하였다. Aniline에서 배양한 Delftia sp. JK-2에서 분리된 약 35kDa의 C2,3O의 N-말단 아미노산 서열을 분석 한 결과 $^1MGVMRIGHASLKVMDMDAAVRHYENV^{26}$로 Pseudomonas sp. AW-2와 Comamonas sp. JS765의 C2,3O와 일치하는 것으로 나타났다. 위에서 확인된 아미노산 서열을 바탕으로 제작된 primer와 JK-2의 total genomic DNA를 기질로 사용하여 PCR을 수행한 결과 약 950 bp의 유전자 증폭산물을 획득하였다. 이 증폭산물 중 정확히 확인된 890 bp의 염기서열을 분석한 결과 Delftia JK-2의 C2,3O유전자 염기서열은 Pseudomonas su. AW-2의 C2,3O와 일치하였으며 Comamonas sp. Js765의 C2,3O와 $97\%$의 높은 상동성을 나타내었다.

• 한국식품과학회지
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• 제36권5호
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• pp.723-727
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• 2004

콩 가공식품인 두유, 두부, 비지에서 유전자변형 콩에 삽입된 epsps 유전자의 검출과 정량을 위해 정성과 정량 PCR 방법을 수행하였다. 두유, 두부, 비지에서 유전자변형 콩의 검출은 삽입된 epsps 유전자의 증폭이 크기가 121bp와 330bp가 생성될 수 있는 두 종류의 primer들을 이용하여 0.01%까지 확인하였다. 정량방법은 1, 3, 5%의 유전자변형 콩이 포함된 시료들을 실시간 PCR을 사용하여 유의성 있는 결과를 얻었다. 이러한 결과들은 실시간 PCR 방법을 사용하여 가공식품 내에서 정량적으로 유전자변형 콩을 정량하는데 적용될 수 있음을 보였다.

• 대한교통학회지
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• 제24권5호
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• pp.19-33
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• 2006

본 연구에서는 NTCIP통신규약을 국내 실시간 교통신호제어시스템에 적용할 수 있는지를 판단하기 위해 기존 신호제어시스템의 통신방식을 NTCIP에 맞게 수정한 후 운영시험을 실시하였다. 시험연구과정에서 한국형 실시간 신호제어시스템용 관리정보집합(MIB)과 OER규칙의 메시지작성라이브러리, SNMP/SFMP 전송관리프로토콜을 지원하는 센터 관리자 S/W및 지역제어기의 대행자소프트웨어가 신규 개발되었다. 또한 국제표준이 되기 전에 국내 적용을 위해 보완되어야 할 문제점들이 도출되었다. 수정된 시스템은 Trap규칙 등 일부 규격의 문제점을 보완하여 적용한 결과 비교적 잘 동작하였다. NTCIP통신규약으로 수정된 시스템의 가용성시험 결과 2400bps에서는 58%의 패킷이 유실되어 정상적인 운영이 불가능하였으나 4800bps와 9600bps에서는 정상적으로 운영되었다. 또한 실시간 제어능력을 평가하는 주기단위 정보전송능력에서는 4800bps이상이 되어야 적합한 것으로 나타났다. 결론적으로 4800bps 이상의 속도에서 정상적인 실시간제어가 가능한 것으로 나타났다. 또 국내환경에 NTCIP통신규약 적용을 위해서는 통신환경 개선이 필요한 것으로 나타났다.

• Journal of Preventive Medicine and Public Health
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• 제44권2호
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• pp.74-83
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• 2011

Objectives: This study aimed to identify and assess the factors related to the awareness, treatment, and control of hypertension based on jurisdictional areas of primary health care posts in a rural community of Korea. Methods: This study was performed on 4598 adults aged over 30 years in a rural community and we measured their blood pressure (BP) from October. 2007 to August. 2009. Hypertension is defined as a condition characterized by a systolic BP ${\geq}$ 140 mmHg, a diastolic BP ${\geq}$ 90 mmHg or reported treatment with antihypertensive medications. We analyzed the factors related with the prevalence, awareness, treatment and control of hypertension using chi-square test and multivariate logistic regression analysis. Results: The age-adjusted prevalence of hypertension was 34.7%. The age-adjusted rates of hypertension awareness, treatment and control were 50.6%, 93.9% and 64.1%, respectively. Awareness of hypertension was related with increasing age. Higher awareness was found among men who were felt more stress, were obese and had hypercholesterolemia, and among women who were regulary taking medicine for hypertension, were obese and had diabetes mellitus. In women, the hypertension treatment was related a Medical aid and education for hypertension management. Controlled hypertension was more common among men who were educated about the management of hypertension and among women who had hypercholesterolemia. Conclusions: The awareness of hypertension was low and the control of hypertension was high compared with the nationwide data (KNHANES 2005). The results suggest that understanding the characteristics of hypertension in a community is important to perform a community based hypertension control program.

• The Plant Pathology Journal
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• 제34권6호
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• pp.514-531
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• 2018

Tomato spotted wilt virus (TSWV; Genus Orthotospovirus: Family Tospoviridae) is one of the most destructive viruses affecting a wide range of horticultural crops on a worldwide basis. In 2015 and 2016, 171 leaf and fruit samples from tomato (Solanum lycopersicum) plants with viral symptoms were collected from the fields in various regions of Iran. ELISA test revealed that the samples were infected by TSWV. The results of RT-PCR showed that the expected DNA fragments of about 819 bp in length were amplified using a pair of universal primer corresponding to the RNA polymerase gene and DNA fragments of ca 777 bp and 724 bp in length were amplified using specific primers that have been designed based on the nucleocapsid (N) and non-structural (NSs) genes, respectively. The amplified fragments were cloned into pTG19-T and sequenced. Sequence comparisons with those available in the GenBank showed that the sequences belong to TSWV. The high nucleotide identity and similarities of new sequences based on the L, N, and NSs genes showed that minor evolutionary differences exist amongst the isolates. The phylogenetic tree grouped all isolates six clades based on N and NSs genes. Phylogenetic analysis showed that the Iranian isolates were composed a new distinct clade based on a part of polymerase, N and NSs genes. To our knowledge, this is the first detailed study on molecular characterization and genetic diversity of TSWV isolates from tomato in Iran that could be known as new clade of TSWV isolates.

• Journal of Veterinary Science
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• 제21권2호
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• pp.22.1-22.9
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• 2020

Rabid raccoon dogs (Nyctereutes procyonoides koreensis) have been responsible for animal rabies in South Korea since the 1990s. A recombinant rabies vaccine strain, designated as ERAGS, was constructed for use as a bait vaccine. Therefore, new means of differentiating ERAGS from other rabies virus (RABV) strains will be required in biological manufacturing and diagnostic service centers. In this study, we designed two specific primer sets for differentiation between ERAGS and other RABVs based on mutation in the RABV glycoprotein gene. Polymerase chain reaction analysis of the glycoprotein gene revealed two DNA bands of 383 bp and 583 bp in the ERAGS strain but a single DNA band of 383 bp in the field strains. The detection limits of multiplex reverse transcription polymerase chain reaction (RT-PCR) were 80 and 8 FAID₅₀/reaction for the ERAGS and Evelyn-Rokitnicki-Abelseth strains, respectively. No cross-reactions were detected in the non-RABV reference viruses, including canine distemper virus, parvovirus, canine adenovirus type 1 and 2, and parainfluenza virus. The results of multiplex RT-PCR were 100% consistent with those of the fluorescent antibody test. Therefore, one-step multiplex RT-PCR is likely useful for differentiation between RABVs with and those without mutation at position 333 of the RABV glycoprotein gene.

• 한국의학물리학회지:의학물리
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• 제33권4호
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• pp.80-87
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• 2022

Purpose: Proton therapy has different relative biological effectiveness (RBE) compared with X-ray treatment, which is the standard in radiation therapy, and the fixed RBE value of 1.1 is widely used. However, RBE depends on a charged particle's linear energy transfer (LET); therefore, measuring LET is important. We have developed a LET measurement method using the inefficiency characteristic of an EBT3 film on a proton beam's Bragg peak (BP) region. Methods: A Gafchromic EBT3 film was used to measure the proton beam LET. It measured the dose at a 10-cm pristine BP proton beam in water to determine the quenching factor of the EBT3 film as a reference beam condition. Monte Carlo (MC) calculations of dose-averaged LET (LET_d) were used to determine the quenching factor and validation. The dose-averaged LETs at the 12-, 16-, and 20-cm pristine BP proton beam in water were calculated with the quenching factor. Results: Using the passive scattering proton beam nozzle of the National Cancer Center in Korea, the LET_d was measured for each beam range. The quenching factor was determined to be 26.15 with 0.3% uncertainty under the reference beam condition. The dose-averaged LETs were measured for each test beam condition. Conclusions: We developed a method for measuring the proton beam LET using an EBT3 film. This study showed that the magnitude of the quenching effect can be estimated using only one beam range, and the quenching factor determined under the reference condition can be applied to any therapeutic proton beam range.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2011년도 정기총회 및 추계학술대회 논문집
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• pp.630-636
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• 2011

Air brake system of KTX effectively distributes air to brake panel in each bogie. System consists of carrying bogie brake panel, motor bogie brake panel, driver's valve panel, pneumatic auxiliary panel and other accessories. For the prove endurance, confirm test number. Distributor and control moderable magnet valve are 500,000 times and respectively, carrying bogie MR/BP cut-out cock are 12,000 times. Tests satisfy all of condition until now. Developments conduct a performance test to brake tester of domestic manufacturer and operating authority.

• Asian-Australasian Journal of Animal Sciences
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• 제17권8호
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• pp.1033-1038
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• 2004

LOD scores related to marbling scores and permutation test have been applied for the purpose detecting quantitative trait loci (QTL) and we selected a considerable major locus BM4311. K-means clustering, for the major DNA marker mining of BM4311 microsatellite loci in Hanwoo chromosome 6, has been tried and five traits are divided by three cluster groups. Then, the three cluster groups are classified according to six DNA markers. Finally, bootstrap test method to calculate confidence intervals, using resampling method, has been adapted in order to find major DNA markers. It could be concluded that the major markers of BM4311 locus in Hanwoo chromosome 6 were DNA marker 100 and 95 bp.

• Journal of the Korean Data and Information Science Society
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• 제18권1호
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• pp.175-184
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• 2007

LOD scores and a permutation test for detecting and locating quantitative trait loci (QTL) from the Hanwoo economic trait have been described and we selected a considerable major BMS1167 locus for further analysis. K-means clustering analysis, for the major DNA marker mining of BMS1167 microsatellite loci in Hanwoo chromosome17, has been tried and three cluster groups divide four traits. The three cluster groups are classified according to eight DNA marker bps. Finally, we employed the bootstrap test method to calculate confidence intervals using the resampling method to find major DNA markers. We conclude that the major marker of BMS1167 locus in Hanwoo chromosome17 is only DNA marker 100bp.