• Asian-Australasian Journal of Animal Sciences
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• 제19권8호
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• pp.1079-1084
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• 2006

To determine whether a link exists between reproductive seasonality and the structure of the melatonin receptor 1A (MTNR1A) gene, the latter was studied in nonseasonal estrous breeds (Small Tail Han and Hu ewes) and seasonal estrous breeds (Dorset, Suffolk and German Mutton Merino ewes). A large fragment of the exon 2 of the MTNR1A gene was amplified and a uniform fragment of 824 bp was obtained in 239 ewes of five breeds. The 824 bp PCR product was digested with restriction endonucleases Mnl I and Rsa I, and checked for the presence of restriction sites. The presence (allele M) or absence (allele m) of an Mnl I site at base position 605 led to three genotypes MM (236 bp/236 bp), Mm (236 bp/303 bp) and mm (303 bp/303 bp) in five sheep breeds. The presence (allele R) or absence (allele r) of a Rsa I site at base position 604 led to three genotypes RR (267 bp/267 bp), Rr (267 bp/290 bp) and rr (290 bp/290 bp) in five sheep breeds. Frequencies of MM and RR genotypes were obviously higher, and frequencies of mm and rr genotypes were obviously lower in nonseasonal estrous sheep breeds than in seasonal estrous sheep breeds. Sequencing revealed four mutations (G453T, G612A, G706A, C891T) in mm genotype compared to MM genotype and one mutation (C606T) in rr genotype compared to RR genotype. For polymorphic Mnl I and Rsa I cleavage sites, the differences of genotype distributions were very highly significant (p<0.01) between Small Tail Han ewes and seasonal estrous sheep breeds. In each group, no significant difference (p>0.05) was detected. These results preliminarily showed an association between MM, RR genotypes and nonseasonal estrus in ewes and an association between mm, rr genotypes and seasonal estrus in ewes.

• 농업과학연구
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• 제27권1호
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• pp.33-38
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• 2000

본 연구는 한우와 Holstein종의 $\kappa$ -CN 유전자 발현조절부위, 즉 $\kappa$ -CN gene 5'-flanking region에 대한 특성을 구명하기 위하여 specific primer를 이용하여 PCR(polymerase chain reaction) 방법으로 분석하였다. PCR 증폭 결과, 349bp 크기의 증폭산물을 얻었으며 한우와 Holstein종의 $\kappa$ -CN 유전자의 발현조절부위의 염기서열 분석결과에 있어서는 -82bp에서 -431bp까지 분석되었으며, 종간의 염기서열 비교에서 -386bp에서는 Holstein종에서는 T 염기가 한우에서는 C 염기로 치환되었으며, -241bp와 -192bp에서는 Holstein종에서는 T 및 C 염가가 존재하였으나, 한우에서는 결실되었다. 또한, -183bp에서는 한우에서는 T 염기가 존재하였으나, Holstein종에서는 염기가 결실되어 있음을 알 수 있었다. 또한 한우와 Holstein 종간의 상동성 분석에 있어서는 한우 $\kappa$ -CN 유전자 발현조절부위의 염기서열이 Holstein종과 98.9% 의 상동성을 보였다.

• Preventive Nutrition and Food Science
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• 제2권2호
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• pp.96-100
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• 1997

Carcinogenicity of di(2-ethylhexyl)phthalate(DEHP) to the mose forestomach and its inhibitor activity for the initiation of Benzo[a]pyrene(BP)-induced mouse forestomach neoplasia were studied on the mouse forestomach carcinogenesis regimen. One hundred female ICR mice(6~7 weeks of age) were hosed in a poly-carbonate cage (4 mice/cage) in a humidity- and temperature-controlled room subjected to a semipurified diet for a week. Mice were divided into 4 treatment groups (25 mice/treatment): Basal diet, DEHP, BP, and BP+DEHP. On Monday and wednesday, 0.1ML DEHP mixed with 0.1ml olive oil (for DEHP and DEHP+BP treatment groups) or 0.1ml saline+0.1ml olive oil (for basal diet group) was intubated, p.o., and on Friday, 2mg BP dissolved in 0.2ml olive oil (for BP and BP+DEHP treatment groups) was intubated, p.o. This cycle was repeated for 4 weeks. Beginning with the first intubation of BP an continuing thereafter, body weight and food intake were recorded once and twice weekly, respectively. All surviving mice were sacrificed 22 weeks after the first dose of BP intubation and countered forestomach tumor. No tumor was formed by DEHP treatment. 5.75 tumors per mouse was formed by BP treatment, whereas its number was reduced to 4.53 by BP+DEHP treatment. Similar results were seen in the tumor incidence. Body weight gain was not affected by DEHP treatment, when compared to that b basal diet treatment. The body weight was significantly reduced by BP treatment, but its reduction was recovered to the level of the basal diet group by BP+DEHP treatment. No significant difference was seen in food intake among all treatment groups. These results indicate that DEHP lacks carcinogenic activity to the mose forestomach and rather inhibits the initiation of BP-induced mose forestomach neoplasia.

• 한국지형학회지
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• 제15권2호
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• pp.67-83
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• 2008

아산 지역에 있어서 최종빙기 최성기 이후 구릉사면의 삭박과정을 밝히기 위하여 매곡천 하류부에 위치한 '아골'의 곡저에서 트렌치 작업을 행하여 채취한 분석시료를 대상으로 층상해석과 탄소연대 측정을 실시하였다. 그 결과는 다음과 같다. 조사지역의 트렌치 MG1, MG2, MG3 지점에서 무기물층과 유기물층은 각각 11매와 8매, 7매와 3매, 5매와 3매가 확인되었다. MG1 지점에서 배후 구릉사면의 불안정한 환경하에 발생했던 사면삭박 즉, 사면물질이동은 총 11회 (약 2,900yrBP 이전에 8회, 약 2,900~1,900yrBP 사이에 2회, 약 1,900yrBP 이후에 1회)이다. 그리고 상대적으로 배후 구릉사면의 안정된 환경하에서 형성된 저습지의 횟수는 최소 총 9회(약 3,000yrBP이전에 5회, 약 3,000~2,800yrBP 사이에 2회, 약 2,200~1,900yrBP 사이에 1회)이다. MG2 지점에서 배후 구릉사면의 삭박은 총 7회(약 1,900yrBP이전에 4회, 약 1,900yrBP 이후에 3회)이고, 저습지의 형성은 총 3회(약 1,900yrBP이전에 2회, 약 1,900yrBP 이후에 1회)이다. MG3 지점에서 배후 구릉사면의 삭박 횟수는 총 5회(약 1,900yrBP이전 3회, 약 1,900yrBP 이후에 2회)이고, 저습지의 형성은 총 3회(약 1,900yrBP 이전에 2회, 약 1,900yrBP 이후에 1회)이다. 따라서 아골 곡저를 둘러싼 구릉사면은 1회가 아닌 수 회의 삭박에 의해 형성되었으며, 다양한 유형의 무기물질이 토사류 또는 토석류와 같은 사면물질이동에 의해 조사지역의 곡저에 매적된 것으로 밝혀졌다. 그리고 구릉사면의 삭박주기는 약 $10^2{\sim}10^3$년 시간규모에 수렴되는 것도 알 수 있었다. 이 결과는 아산 주변에 분포하는 구릉사면의 삭박과정 및 제4기 후기의 기후변화 복원에 중요한 기초자료가 될 것이다.

• 경영정보학연구
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• 제6권1호
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• pp.103-121
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• 2004

최근에 경쟁우위를 창출하고 보다 나은 재무적 성과를 확보하는데 있어서 정보시스템 계획과 사업 계획을 통합시키는 효익을 평가할 필요성이 증대되고 있다. 이러한 사업 계획과 정보시스템 계획의 통합유지가 정보시스템 관리자들에게 중대한 관심분야로 자주 언급되고 있지만, 조직의 사업 계획과 정보시스템 계획이 통합되지 못하고 있어 조직의 전략을 효과적으로 지원하지 못하고 있는 것이 일반적인 현실이다. 또한, BP-ISP의 연계에 관한 연구는 많이 행해졌지만, BP-ISP 통합의 필요성이 선행 연구에 의해 명확하게 제시되고 있음에도 불구하고 BP-ISP의 통합에 관한 연구, 특히 완전 통합을 이루기 위한 방안에 관한 연구는 이루어지지 않고 있는 실정이다. 이에 본 연구에서는 Teo와 King(1996; 1997)이 제시한 바와 같이 BP-ISP 통합 단계를 별개의 관리적 통합(type1), 일방향 순차적 통합(type 2), 쌍방향 상호적 통합(type 3), 완전 통합(type 4)으로 구분하여 이를 토대로 설문조사를 통해 우리나라 보험산업이 현재 BP-ISP 통합 단계 중 위치한 단계를 살펴보고, 쌍방향 상호적 통합(type3) 또는 완전 통합(type 4) 단계에 있는 기업을 대상으로 인터뷰를 실시하여 기존 연구들이 간과한 BP-ISP 통합의 저해 요인 및 성공 요인을 밝히는 연구를 수행하였다. 본 연구의 결과는 BP-ISP 통합을 고려하고 있는 기업들에게 통합을 성공적으로 이루기 위한 요인을 제시해주고 있으며, 앞으로 우리나라 기업을 대상으로 BP-ISP 통합 단계를 진단해 보고, 실제로 BP-ISP 통합이 조직의 성과에 기여하는가, 또한 본 연구에서 제시한 저해요인과 성공요인이 실제로 통합에 중요한 영향을 미치는가 등에 관한 연구를 진행할 수 있을 것으로 기대된다.

• Asian-Australasian Journal of Animal Sciences
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• 제15권3호
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• pp.421-426
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• 2002

The pyruvate dehydrogenase complex (PDC), a member of $\alpha$-keto acid dehydrogenase complex, catalyzes the oxidative decarboxylation of pyruvate with the formation of $CO_2$, acetyl-CoA, NADH, and $H^+$. This complex contains multiple copies of three catalytic components including pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2), and dihydrolipoamide dehydrogenase (E3). Two regulatory components (E1-kinase and phospho-E1 phosphatase) and functionally less-understood protein (protein X, E3BP) are also involved in the formation of the complex. In this study, we have partially cloned the gene for E3BP in human. Nine putative clones were isolated by human genomic library screening with 1.35 kb fragment of E3BP cDNA as a probe. For investigation of cloned genes, Southern blot analysis and the construction of the restriction map were performed. One of the isolated clones, E3BP741, has a 3 kb-SacI fragment, which contains 200 bp region matched with E3BP cDNA sequences. The matched DNA sequence encodes the carboxyl-terminal portion of lipoyl-bearing domain and hinge region of human E3BP. Differences between yeast E3BP and mammalian E3BP coupled with the remarkable similarity between mammalian E2 and mammalian E3BP were confirmed from the comparison of the nucleotide sequence and the deduced amino acid sequence in the cloned E3BP. Cloning of human E3BP gene and analysis of the gene structure will facilitate the understanding of the role(s) of E3BP in mammalian PDC.

• Psychiatry investigation
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• 제15권12호
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• pp.1135-1143
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• 2018

Objective The aim of this study was to evaluate differences in psychopathology between offspring of parents with bipolar I disorder (BP-I) and those with bipolar II disorder (BP-II). Methods The sample included 201 offspring between 6 and 17 years of age who had at least one parent with BP-I or BP-II. The offspring were diagnostically evaluated using the Korean Kiddie-Schedule for Affective Disorders and Schizophrenia-Present and Lifetime Version. Psychopathology and Clinical characteristics were evaluated, including lifetime DSM-5 diagnoses, depression, and childhood trauma. Lifetime DSM-5 diagnoses were also compared between schoolchildren aged 6 to 11 years and adolescents aged 12 to 17 years. Results In lifetime DSM-5 diagnoses, offspring of parents with BP-I had significantly increased risk of developing MDD and BP-I than those with BP-II. Regarding clinical characteristics, ADHD rating scale and childhood trauma scale were significantly higher in offspring of parents with BP-I than that in those with BP-II. Conclusion The present study supports that BP-I may be etiologically distinct from BP-II by a possible genetic liability. Our findings indicate that additional research related to bipolar offspring is needed to enhance understanding of differences between BP-I and BP-II.

• 생체재료학회지
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• 제22권4호
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• pp.352-359
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• 2018

Background: Black phosphorus (BP) has emerged as a novel class of nanomaterials owing to its unique optical and electronic properties. BP, a two-dimensional (2D) nanomaterial, is a structure where phosphorenes are stacked together in layers by van der Waals interactions. However, although BP nanodots have many advantages, their biosafety and biological effect have not yet been elucidated as compared to the other nanomaterials. Therefore, it is particularly important to assess the cytotoxicity of BP nanodots for exploring their potentials as novel biomaterials. Methods: BP nanodots were prepared by exfoliation with a modified ultrasonication-assisted solution method. The physicochemical properties of BP nanodots were characterized by transmission electron microscopy, dynamic light scattering, Raman spectroscopy, and X-ray diffractometry. In addition, the cytotoxicity of BP nanodots against C2C12 myoblasts was evaluated. Moreover, their cell imaging potential was investigated. Results: Herein, we concentrated on evaluating the cytotoxicity of BP nanodots and investigating their cell imaging potential. It was revealed that the BP nanodots were cytocompatible at a low concentration, although the cell viability was decreased with increasing BP nanodot concentration. Furthermore, our results demonstrated that the cells took up the BP nanodots, and the BP nanodots exhibited green fluorescence. Conclusions: In conclusion, our findings suggest that the BP nanodots have suitable biocompatibility, and are promising candidates as fluorescence probes for biomedical imaging applications.

• 한국해양생명과학회지
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• 제2권1호
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• pp.34-38
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• 2017

FK506BP는 일명 FK506 binding protein 12이라 불리는 작은 펩티드로서 single 도메인을 가진다. FK506BP는 면역반응 억제, 산화적 스트레스 및 염증과 관련이 있다. 본 연구의 목적은 참돔(Pagrus major)을 저수온(8℃, 33 psu) 및 저염분(20℃, 10 psu) 상태에 노출시킨 후, FK506BP 유전자의 발현을 관찰하는 것이다. 연구결과, FK506BP 유전자의 발현은 저수온(8℃, 33 psu) 및 저염분(20℃, 10 psu)상태에서 유의적으로 증가하는 것으로 나타났다. 이 연구결과로서 FK506BP 유전자는 수온 및 염분 등의 환경 스트레스에 대한 생체지표유전자로서 역할을 한다고 제의한다.

• 대한수의학회지
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• 제58권3호
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• pp.131-136
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• 2018

Blood pressure (BP) measurement plays a pivotal role in veterinary medicine for diagnosing cardiovascular disorders and monitoring anesthesia of animals. Although indirect BP measurement has been widely applied to monitor BP because of its convenience and non-invasiveness, it is still unclear whether indirect BP measurement is compatible with direct BP measurement in minipigs. In addition, the effect of animal posture during BP measurement is not well understood in minipigs despite its importance to cardiovascular performance. Therefore, both systolic and diastolic arterial BPs in minipigs were measured via femoral artery catheterization for direct BP measurement and using a compressive cuff as an indirect BP measurement under the dorsal or right lateral recumbent postures. Numerical values were processed by the Bland-Altman method to calculate the bias ${\pm}$ SD and the limits of agreement (LOA). In accordance with the American College of Veterinary Internal Medicine guidelines, the results between direct and indirect BP measurements were determined as apparent disagreements in both systolic and diastolic arterial BPs under all postures because of large bias ${\pm}$ SD and wide LOA. The results of the present will help prevent misinterpretation of the anesthetized patient's condition during monitoring of BP by indirect measurement.