• Journal of Ginseng Research
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• v.13 no.1
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• pp.42-48
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• 1989

The binding of bay region diol-epoxides of polycyclic aromatic hydrocarbons (PAHs) to target tissue DNA is thought to be essential for the initiation of cancer by these compounds. In this study we investigated the effect of polyacetylenes such as panaxynol and panaxydol on the formation of benzo(a)pyreno (BP)-metabolite-DNA adduct in the liver of ICR mice. Treatment of mice by i.p. administration of polyacetylenes produced a marked reduction in BP metabolite binding to DNA in vitro. Following i.v. administration of (3H)BP(300, ${\mu}$Ci/21 nmoles/0.1 nt DMSO) to mice, radioactivity was detected in the DNA of the liver in vivo. The result of tentative identification of the 4 peaks between the two standard markers for high pressure liquid chromatography showed that the peaks. I, II, III, and IV were BP-phenol oxide-DNA adduct (or BP-diol-epoxide-dCyt. adduct), (-) BP$.$diolepoxide I:dGuO adduct, (+) BP-diol-epoxide I: dGuo adduct, and BP-diol-epoxide II:dGuO adduct, respectively. The minor adduct, (-) BP-diol epoxide I: dGuo was reduced to 6971 of the amount of the control, while the major adduct, (+) BP-diolepoxide I: dGuO(peak II) which was produced from (-) BP-7, 8-diol was reduced to 78% of that of the control. The amount of the minor adduct, BP-diol-epoxide II:dGuo adduct(peak IV) which formed from (+) BP-7, 8-diol was 58% of the control. These results show that the panaxydol is more related to inhibition of the formation of the minor ad- ducts than of the major adducts, which were generally produced from ($\pm$) BP-7, 8-dihydro-dials.

• Journal of Life Science
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• v.26 no.9
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• pp.1007-1014
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• 2016

A highly efficient, rapid, and reliable multiplex polymerase chain reaction based method for distinguishing ten species of genus Cynoglossus (C. senegalensis, C. abbreviates, C. macrolepidotus, C. arel, C. semilaevis, C. interruptus, C. joyneri, C. lingua, C. robustus, and C. monodi) is described. The species-specific primer sets were designed base on the cytochrome oxidase subunit I gene (1,500 bp). The optimal PCR conditions and primers were selected for ten of Cynoglossus species to determine target base sequences using single PCR. Multiplex PCR using the ten pairs of primers either specifically amplified a DNA fragment of a unique size or failed, depending on each species DNA. The length of amplification fragment of 208 bp for C. senegalensis, 322 bp for C. abbreviates, 493 bp for C. macrolepidotus, 754 bp for C. arel, 874 bp for C. semilaevis, 952 bp for C. interruptus, 1,084 bp for C. joyneri, 1,198 bp for C. lingua, 1,307 bp for C. robustus, and 1,483 bp for C. monodi with the species-specific primers, visualized by agarose gel electrophoresis, allowed perfectly distinction of the Cynoglossus species. The multiplex PCR assay can be easily performed on multiple samples and attain final results in less than 6 hours. This technique should be a useful addition to the molecular typing tools for the tentative identification of Cynoglossus species.

• ETRI Journal
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• v.26 no.5
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• pp.432-436
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• 2004

In this paper, we propose the modified uniformly most powerful (UMP) belief-propagation (BP)-based decoding algorithm which utilizes multiplicative and additive factors to diminish the errors introduced by the approximation of the soft values given by a previously proposed UMP BP-based algorithm. This modified UMP BP-based algorithm shows better performance than that of the normalized UMP BP-based algorithm, i.e., it has an error performance closer to BP than that of the normalized UMP BP-based algorithm on the additive white Gaussian noise channel for low density parity check codes. Also, this algorithm has the same complexity in its implementation as the normalized UMP BP-based algorithm.

• Journal of Ecology and Environment
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• v.29 no.6
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• pp.573-580
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• 2006

A sediment core from the Cheollipo arboretum ($36^{\circ}$ 47' 57'N, $126^{\circ}$ 09' 04') was studied for pollen analysis in order to reconstruct postglacial vegetational change and environmental changes around the central western region of the Korean Peninsula. The record shows four pollen assemblage zones: Zone CHL-I, Quercus stage (ca. 9,300$\sim$6,200 yr BP): zone CHL-II, Quercus-Pinus stage (ca. 6,200$\sim$4,600 yr BP); zone CHL- III, Pinus-Quercus stage (ca. 4,600$\sim$1,160 yr BP): zone CHL-IV and Pinus stage (ca. 1,160 yr BP-present). During the 9,300$\sim$8,500 yr BP, the early Holocene, researchers have guessed a piece of cool-temperate norihern/altimontane mixed coniferous and deciduous broad-leaved forest. Between 8,500$\sim$4,600 yr BP the Quercus dominated the landscape of study area and the established dates of this typical cool-temperate central/montane deciduous broad-leaved forest vegetation might be ca. 6500 yr BP, and then the Pinus developed around the site at ca. 5,700 yr BP. The abrupt increase of Pinus and NAP (non-arboreal pollen) after ca. 1,100 yr BP indicates the vegetation changes due to human activities. From the dynamics of the Chenopodiaceae pollen indicating salt marsh and freshwater pollen flora such as Typha, Trapa, Nymphoides and so forth, we suggest that the tidal flat was altered into freshwater lake around 6,500 yr BP.

• Annals of Clinical Neurophysiology
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• v.15 no.1
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• pp.1-6
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• 2013

Background: Bell's palsy (BP) is the most common cause of unilateral lower motor facial palsy. Recurrent paralysis of the facial nerve is unusual and reported in only 7-8%. Methods: A total of 394 consecutive patients with acute BP patients were enrolled at Daegu Catholic University Hospital from July 2005 to September 2012. We classified the patients into two groups-single BP and recurrent BP-and compared them by patient characteristics, clinical features, MRI findings, electrophysiologic findings and prognosis. The degree of BP was graded according to the House and Brackmann facial nerve grading system. Results: Recurrent BP was observed in 31 (7.9%) patients. The number of recurrence was varied from 2 to 5. The recurrent BP (9.7%) had more incidence of family history and MRI enhancement than those of single BP (2.2%, p=0.047). The single BP (63.4%) had better recovery than recurrent BP (45.2%, p=0.045). Conclusions: The recurrent BP had more incidence of family history, MRI enhancement and poor prognosis than the single BP.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.56 no.4
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• pp.293-298
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• 2011

Physical and structural characteristics of four local barnyardgrass flour and starch were investigated. Amylose content ranged from 6.9 to 12.5%. The BP 3563 and BP 3606 were low amylose lines, and the BP 3592 and BP 3610 showed very low amylose lines. Peak viscosities of four barnyardgrass collections BP-3563, BP-3592, BP-3606, and BP-3610 ranged 264 to 2397. All barnyard grass lines displayed an A-type X-ray diffraction pattern showing the strong diffraction peak at around $2{\theta}$ values $15^{\circ}$, $17^{\circ}$, $18^{\circ}$ and $23^{\circ}$, and a small peak at $20^{\circ}$ $2{\theta}$. Scanning electron microscopy revealed that the starch granule sizes ranged $5.5-12.5{\mu}m$ in diameter showing polygonal and rarely spherical in shape with round edges and some pores at the surface. Starch crystallinity of BP 3563, BP 3592, BP 3606, and BP 3610 was 23.9, 25.0, 24.7, and 25.5%, respectively.

• Asian-Australasian Journal of Animal Sciences
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• v.11 no.1
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• pp.71-77
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• 1998

The experiment was conducted to evaluate Bio-V-Pro as an alternative protein source in broiler diets. Treatments were control, Bio-V-Pro 1% (BP 1), Bio-V-Pro 3% (BP 3), and Bio-V-Pro 5% (BP 5). During the starter period, all treated groups showed beter performance than control group in weight gain and feed intake (p < 0.05). The best FCR was observed in BP 1 group and the worst was found in BP 5 group. For the overall period, chicks fed BP 1 and BP 3 diets gained more than the chicks fed the control and BP 5 diets. All Bio-V-Pro fed groups consumed more feed than the control group (p < 0.05). FCR were similar between the control and BP 1 group, but BP 3 and BP 5 groups showed higher FCR than the control group. During the starter period, all BP groups showed better utilizability of crude protein, crude ash and phosphorus than the control group (p < 0.05). For the finisher period, except for the BP 5 group, the nutrient utilizability was not significantly different in most nutrients. Nutrients utilizability had a tendency (not significant) to decrease as the level of Bio-V-Pro in the diet was increased. Lysine utilizability was not affected by the dietary treatment, however, methionine utilizability was higher in BP 1 and BP 3 group than in the control group. From the results of this experiment, it seems that Bio-V-Pro is a good alternative protein source which can replace fish meal in starter diet up to 3% of the diet effectively.

• Journal of Life Science
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• v.8 no.1
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• pp.32-39
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• 1998

The length and G/C concent of regions of an aphid rDNA unit that spans 13,061bo with 59% G/C content. flolowing belowing below are the those results, 5’ETS is 843bp in length with 69% G/C content, 18S is 2,469bp in length with 59% G/C content, ITS I is 229bp in length with 70% G/C content, 5.8S is 160bp in length with 63% G/C content, ITS II is 325bp in length with 70% G/C content, 28S is 4, 147bp in length with 60% G/C content, IGS is 4,888bp in length with 55% G/C content.

• Journal of Ginseng Research
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• v.13 no.1
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• pp.37-41
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• 1989

Reactive metabolites generated by benzo(a)pyrene(BP) monooxygenase(AHH) interact with nucleophiles in DNA and cause mutation and carcinogenesis. We studied the effect of Panax ginseng C.A. Meyer, which induce epoxide hydratase(EH) activity without concomitant induction of AHH activity, on the binding of BP metabolites to DNA in uitro in Sprague Dawley rats. DNA-BP metabolite adducts can be resolved into at least five distinct peaks by elution of a Sephadex LH-20 column with a water methanol gradieNt. These peaks are arbitrarily designated A(most polar) through I(least polar). Of the 5 peaks tentatively assigned to 7,8 biol-9,10-oxide(A),7,8·oxide(B),4,5-oxide(C), and further metabolites of 9-OH-BP(D & E), peaks A, C, D, and I were reduced to 70, 85, 80, and 30% of controls, respectively, and there was no significant change in peak B. In connection with this DNA binding study, BP metabolizing enzymes including AHH, EH, demethylase(DM) activity and cyt. P-450 contents were also investigated in order to compare the BP treated control with ginseng and BP treated test groups. The results showed that the EH activity was increased by 139% over the BP control, the Cyt. P-450 content was increased by 180% over the control value, and DM and AHH activities were also increased to some degree for the BP test group, but there was no significant effect of the ginseng treatment.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.8
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• pp.1079-1084
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• 2006

To determine whether a link exists between reproductive seasonality and the structure of the melatonin receptor 1A (MTNR1A) gene, the latter was studied in nonseasonal estrous breeds (Small Tail Han and Hu ewes) and seasonal estrous breeds (Dorset, Suffolk and German Mutton Merino ewes). A large fragment of the exon 2 of the MTNR1A gene was amplified and a uniform fragment of 824 bp was obtained in 239 ewes of five breeds. The 824 bp PCR product was digested with restriction endonucleases Mnl I and Rsa I, and checked for the presence of restriction sites. The presence (allele M) or absence (allele m) of an Mnl I site at base position 605 led to three genotypes MM (236 bp/236 bp), Mm (236 bp/303 bp) and mm (303 bp/303 bp) in five sheep breeds. The presence (allele R) or absence (allele r) of a Rsa I site at base position 604 led to three genotypes RR (267 bp/267 bp), Rr (267 bp/290 bp) and rr (290 bp/290 bp) in five sheep breeds. Frequencies of MM and RR genotypes were obviously higher, and frequencies of mm and rr genotypes were obviously lower in nonseasonal estrous sheep breeds than in seasonal estrous sheep breeds. Sequencing revealed four mutations (G453T, G612A, G706A, C891T) in mm genotype compared to MM genotype and one mutation (C606T) in rr genotype compared to RR genotype. For polymorphic Mnl I and Rsa I cleavage sites, the differences of genotype distributions were very highly significant (p<0.01) between Small Tail Han ewes and seasonal estrous sheep breeds. In each group, no significant difference (p>0.05) was detected. These results preliminarily showed an association between MM, RR genotypes and nonseasonal estrus in ewes and an association between mm, rr genotypes and seasonal estrus in ewes.