• Korean Journal of Microbiology
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• v.32 no.1
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• pp.70-77
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• 1994

The author isolated 7 mutant candidates which mapped around cysA (which was 10 o'clock). They were divided into two groups. One of them was located counterclockwise to cysA, and the other was clockwise to cysA. Since bldA was mapped counterclockwise to cysA, the candidate which mapped counterclockwise to cysA was transduced with phage containing wild type bldA gene clone. The candidates might be the alleles of bldA, because they were complemented by bldA clone. However some of such mutants sporulated very well and developed as much pigment as wild type on rich media plate. Their phenotype was not like bld mutant at all on such conditions. There were real antibiotics gene expressions, since transcriptional reporter gene xylE had shown high activities. Majority of the bldA like mutants showed act gene expressions when they were transformed with high copy number plasmid containing actII-ORF4.

• Journal of the Korean Magnetic Resonance Society
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• v.17 no.1
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• pp.59-66
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• 2013

BldD, a developmental transcription factor from Streptomyces coelicolor, is a homodimeric, DNA-binding protein with 167 amino acids in each subunit. Each monomer consists of two structurally distinct domains, the N-terminal domain (BldD-NTD) responsible for DNA-binding and dimerization and the C-terminal domain (BldD-CTD). In contrast to the BldD-NTD, of which crystal structure has been solved, the BldD-CTD has been characterized neither in structure nor in function. Thus, in terms of structural genomics, structural study of the BldD-CTD has been conducted in solution, and in the present work, mainchain NMR assignments of the recombinant BldD-CTD (residues 80-167 of BldD) could be achieved by a series of heteronuclear multidimensional NMR experiments on a [$^{13}C/^{15}N$]-enriched protein sample. Finally, the secondary structure prediction by CSI and TALOS+ analysis using the assigned chemical shifts data identified a ${\beta}-{\alpha}-{\alpha}-{\beta}-{\alpha}-{\alpha}-{\alpha}$ topology of the domain. The results will provide the most fundamental data for more detailed approach to the atomic structure of the BldD-CTD, which would be essential for entire understanding of the molecular function of BldD.

• Proceedings of the Korean Biophysical Society Conference
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• 2002.06b
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• pp.49-49
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• 2002

Mutants blocked at the earliest stages of morphological development in Streptomyces species are called bld mutants. We have cloned bldB gene ORF from Slividans. Genomic Southern blot analysis for main strains S.lividans, S.seoulensis, S.coelicolor A3(2), and S.griseus indicated that bldB gene is conserved in all main Streptomyces strains.(omitted)

• Korean Journal of Optics and Photonics
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• v.5 no.1
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• pp.106-112
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• 1994

The injection-locking of the broad-area diode laser was analysed by using the Fabry-Perot model. The far-field pattern of the output beam was able to be treated by superposing the individual beams emitted from the front facet due to the multiple reflections between the front and rear facets. and the exact near and far field patterns were obtained. The angle-steering effect according to change of the incident frequency was changed was calculated and found to be 0.022 degree/GHz with a bandwidth of 120 GHz.20 GHz.

• Proceedings of the Korean Biophysical Society Conference
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• 2003.06a
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• pp.49-49
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• 2003

BldD is one of a set of morphological differentiation regulators in Streptomyces coelicolor. The bldD coding region was cloned and heterologously expressed in Escherichia coli. Previous analysis showed the splitting appearance of purified BldD.

• Journal of Life Science
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• v.22 no.3
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• pp.347-353
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• 2012

The purpose of this study was to research the cosmeceutical activity of 70% ethanol extracts when Brassica oleracea var. italica Plenck (BO) was dried under red lighting (RLD), blue lighting (BLD), red+blue lighting (RBLD), or white lighting (WLD). The electron-donating abilities of 70% ethanol extracts from drying BO under RLD, BLD, RBLD, and WLD were 62.8%, 68.1%, 60.9%, and 69.9%, respectively, at a concentration of 500 ppm. The superoxide dismutase (SOD)-like activities of 70% ethanol extracts from drying BO under RLD, BLD, RBLD, and WLD were 39.2%, 47.2%, 19.6%, and 21.6%, respectively, at a concentration of 1,000 ppm. In terms of xanthine oxidase inhibition effects, the proportions of 70% ethanol extracts after drying BO under RLD, BLD, RBLD, and WLD were 45.1%, 56.2%, 38.8%, and 53.3%, respectively, at a concentration of 1,000 ppm. The tyrosinase inhibition effects of 70% ethanol extracts when BO was dried under RLD, BLD, RBLD, and WLD were 21.0%, 13.1%, 26.0%, and 19.1%, respectively, at a concentration of 1,000 ppm. The collagenase inhibition effect of 70% ethanol extracts when BO was dried under RLD, BLD, RBLD, and WLD were 47.4%, 20.8%, 54.6%, and 37.2%, respectively, at a concentration of 1,000 ppm. The astringent inhibition effects of 70% ethanol extracts when BO was dried under RLD, BLD, RBLD, and WLD were 21.5%, 39.6%, 40.0%, and 51.6%, respectively, at a concentration of 5,000 ppm. All of these findings suggest that BO extracts dried under RLD, BLD, RBLD, and WLD have great potential as a cosmeceutical ingredient with good biological activity.

• Korean Journal of Microbiology
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• v.33 no.2
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• pp.149-156
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• 1997

We have employed comparative RFL,P(Restriction Fragment Ixngth Pol~iniorphism) analysis and molecular phylogenetic techniques to investigate the diversity of uncultured microorganisms associated with the anaerobic PCP degradation in PCP-adapted enrichment cultures inoculated by samples from anaerobic cewage sludgc(Jangrim, Pusan) and leachate of landfill site(Kimhae). 16s rDNA cloncs were obtairted by PCR amplification of mixed population DNAs extracted directly from the nonactive and active stage ol each PCP-adapted culture. After three rounds of comparative RFLP analyses. two RFLP types. designated as Ala and Hld, were found prevalent and common in both active stage samples. Thc analysis of phylogenctic diversity bawd on the 5'-terminal 180 nt of sequences from whole clones of the Ala and Bld RFLP types showed close similarity among themselves. In case of Bld clones, 7XQ of them shared identical sequences. Thcse resuliq suggest that the clones of both RFLP types wcre originated from highly affiliated microorganisms which are e~iriched as a result of metabolic activity to PCP. The full-length 16s rRNA sequence of each representative clone from both RFLP types was determined. and an Ala clone w i n found to he related to Clo.strrdiurn ulfutzac~(Genk~ank No. Z69203) and a Bld clone to Thermobacteroides proteolyticus(Genbank No. X09335), with sequence similarities of 89%' and 97%. respectively.

• Asian Journal of Business Environment
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• v.2 no.2
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• pp.27-33
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• 2012

Purpose - The article studies aims to construct the center of economy in the upriver area of Chang Jiang, and has realistic significance probing into the contribution of insurance essential factor market to economic development on the contribution role of essential factor market of insurance in financial industry development to economic growth in Chongqing in both aspects of direct and indirect contribution by the way of demonstration analysis. Research data and methodology - The data are from Statistic Yearbook in Chongqing in 1997-2008.The conclusion shows that essential factor market of insurance development falls behind of economic growth in direct aspect; BBD, BLD and FIR could pull economic growth, but ID just restrain economic growth in Chongqing. Results -The estimate coefficient sigh of BDD, BLD, FIR are plus but ID is not, it is to say the increase of bank deposit dump could impel economic growth, which is accord with general thought. Conclusions - At last, the article Having Studied on the contribution role of essential factor market of insurance in financial industry development to economic growth in Chongqing by the way of demonstration analysis.

• Journal of Korean Physical Therapy Science
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• v.31 no.1
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• pp.46-52
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• 2024

Background: This study aims to investigate compensatory strategy in a dog with glenoid dysplasia using kinetic gait analysis before and after reconstruction of medial patellar luxation. Design: Case report Method: On the platform, gait analysis was evaluated for a dog with congenital luxation and bilateral medial patellar luxation (MPL). A dog was evaluated for maximal vertical force (MVF), body load distribution (BLD), and symmetry index (SI), including the left forelimb with congenital luxation before MPL surgery, 15 days of surgery, and 40 days of surgery. Result:: In the comparison between the preoperative and the 15 days of surgery, the MVF of the bilateral forelimbs, especially in the non-affected forelimbs, increased, and the SI also increased. For BLD, the maximum load distribution increased, but the total load distribution decreased. In the comparison of 15 days and 40 days of surgery, MVF and BLD increased, and SI decreased on 40days of surgery. Conclusions: In a dog, the shift in weight load to the non-affected side occurs all the limbs, affecting the peak vertical force, weight load distribution, and symmetry index.

• Journal of Mechanical Science and Technology
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• v.18 no.6
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• pp.946-954
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• 2004

A model in the form of a Bearing Load Distribution (BLD) matrix in the Multi Bearing Rotor System (MBRS) is established by a transfer matrix equation with the consideration of a bearing load, elevation and uniform load distribution. The concept of Bearing Load Sensitivity (BLS) is proposed and matrices for load and elevation sensitivity are obtained. In order to share MBRS design resources on the Internet with remote customers, the basic principle of Remote Computing (RC) based on the Internet is introduced ; the RC of the BLD and BLS is achieved by Microsoft Active Server Pages (ASP) technology.