• 한국임상수의학회지
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• 제22권3호
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• pp.202-205
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• 2005

본 연구는 개에서 위수혈(BL-21) 및 중완혈(CV-12)에 대한 지침의 구토억제효과를 규명할 목적으로 수행하였다. 사용 혈위는 BL-21 및 CV-12이었다. 각 군에서 구토 유발을 위하여 $2{\%}$ xylazine(2.2 mg/kg)을 투여하였으며, metoclopramide 수침은 1 mg/kg의 비율로 해당 혈위에 각각 수침하였다 대조군 및 실험군에 있어서 xylazine 투여 20분전에 BL-21 및 CV-12에 자침, metoclopramide 수침 및 뜸 처치를 각각 실시하였다. 대조군 및 실험군에서 구토율 및 구토발현시간을 각각 조사한 결과는 다음과 같았다. BL-21자침군, CV-12 자침군 및 BL-21과 CV-12 병용 자침군의 구토율은 각각 $33.3{\%}\;50.0{\%}$ 및 $33.3{\%}$로서 대조군($66.7{\%}$)보다 낮았다. BL-21 자침군, CV-12 자침군 및 BL-21과 CV-12 병용 자침군에 있어서 구토 발현 시간은 대조군과 유사하였다. BL-21 수침군 및 CV-12 수침군의 구토 억제율은 각각 $100{\%}$ 및 $83.6{\%}$로서 수침군의 대조군($63.6{\%}$)보다 높았다. 구토발현시간은 수침군의 BL-21 수침군은 0분이었으며, CV-12 수침군은 5분으로서 수침군의 대조군과 유사하였다. BL-21 뜸 처치군 및 CV-12뜸 처치군의 구토율은 각각 $33.3{\%}$로서 뜸 처치군의 대조군($66.7{\%}$)보다 낮았다. 구토발현시간은 뜸 처치군의 대조군 $2{\~}4$분, BL-21 뜸 처치군이 $2{\~}4$분 및 CV-12 뜸 처치군이 $2{\~}3$분으로서 큰 차이를 나타내지 않았다. 이상의 결과를 종합해 볼 때, 개에서 $2{\%}$ xylazine 투여로 유발된 구토에 대한 BL-21 및 CV-12의 자침, metoclopramide 수침 및 뜸 처치가 각각 구토 억제에 효과적이었으며, 또한 이들 중 BL-21에 대한 metoclopramide 수침의 구토억제 효과가 가장 우수하였다.

• Journal of Microbiology and Biotechnology
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• 제30권10호
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• pp.1592-1596
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• 2020

The aerobic growth and metabolic performance of Escherichia coli strains BL21 and W3110 were studied when the Vitreoscilla hemoglobin (VHb) was constitutively expressed in the chromosome. When VHb was expressed, acetate production decreased in both strains and was nearly eliminated in BL21. Transcriptional levels of the glyoxylate shunt genes decreased in both strains when VHb was expressed. However, higher transcription of the α-ketoglutarate dehydrogenase genes were observed for W3110, while for BL21 transcription levels decreased. VHb expression reduced the transcription of the cytochrome bo₃ genes only in BL21. These results are useful for better selecting a production host.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVII)
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• pp.663-667
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• 2005

Water-soluble glucan was produced by mutants of E. coli BL21(DE)/CrdS-F and E. coli BL21(DE)/CrdS-C in a fermentor. Mutants of E. coli BL21(DE)/CrdS-F which has putative ${\beta}-1,3-glucan$ synthase catalytic subunit (gi:40556679) gene and E. coli BL21(DE)/CrdS-C which contains the active catalytic domain of partial curdlan synthase gene. The molecular weight of water-soluble glucan was analysed with HPLC.

• Korean Journal of Acupuncture
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• 제29권4호
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• pp.604-615
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• 2012

Objectives : The aim of this study was to observe the effect of moxibustion at combined acupoints of ST36, BL21 and CV12 in rats with sex and age. Methods : This study measured small intestinal motility in rats. First, cauterize with moxa was applied 5 times to the ST36, BL21 and CV12 in rats classified by sex and age under enflurane anesthesia. And then same treatment is done to mached acupoints of ST36, BL21 and CV12. Results : In single acupoint groups, cauterizing with moxa on ST36 increased with 5, 7 weeks in female and all weeks in male. The BL21 group increased with 5, 6, 7 weeks in female, and only 5 weeks in male. The CV12 group showed increasing with 5, 6 weeks in female and only 7 weeks in male. In combined acupoints groups, the ST36+BL21 with 8 weeks in male, the ST36+CV12 with 5, 8 weeks in female and 5, 6, 8 weeks in male, the CV12+BL21 only with 5 weeks in female, the ST36+BL21+CV12 only with 5 weeks in female showed increasing, respectively. Conclusions : Although these differents according to the sex and age in rats do not have a established tendency, the results suggested that the effects of moxibustion are experimentally should be considered the sex and age of individual.

• Journal of Acupuncture Research
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• 제20권2호
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• pp.135-144
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• 2003

Objective : The purpose of this study is To investigate the effects of acupuncture and moxibustion at Zusanli(ST36) and Weishu(BL21) Methods : serum gastrin level by radioimmunoassay was measured at 5 days after acupuncture and moxibustion of those acupoints. Gastric endocrine cell(G cell and Histamine immunoreactive density) by avidin-biotinylated complex(ABC) technique, histological examinations(Alcian Blue-PAS Stain; Alcian blue-Periodic Acid Schiff reagent) of the gastric mucosa were also performed. Acupuncture applied to the ST36 acupoint increased gastrin level of serum, but moxibustion did not produced significant effect. All of acupuncture and moxibustion at BL21 acupoint increased gastrin level of serum significantly. In moxibustion at ST36 and BL21, the number of gastrin secreting cells in gastric mucosa, the density of immunoreactive histamin secreting cells and the density of body mucosa stained by PAS were decreased compare to acupuncture at ST36 and BL21. In acupuncture and moxibustion at BL21, the density of pylorus mucosa stained by PAS were increased compare to the groups applied to ST36. In the density of body mucosa stained by AB, moxibustion at BL21 and ST36 were increased compare to the other groups. Results : These data suggest that acupuncture and moxibustion at BL21 increased gastrin level of serum and those effects were more potent than acupuncture at ST36.

• 대한수의학회지
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• 제45권3호
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• pp.399-403
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• 2005

This study was performed to elucidate the effect of acupuncture and aqua-acupuncture in dogs. Each group was divided into control (18 dogs), PC-6 (6 dogs) and BL-21 (6 dogs) group for the antiemetic effect of acupuncture, respectively. And, the animals were divided into control (18 dogs), PC-6 (18 dogs) and BL-21 (18 dogs) group for the antiemetic effect of aqua-acupuncture with metoclopramide, respectively. 2% xylazine was injected to the all dogs to induce the emesis, and acupuncture and aqua-acupuncture treatment (0.5% metoclopramide) were applied to animals for 20 min. before xylazine injection, respectively. Emetic rates in PC-6 (33.3%) and BL-21 (16.7%) were lower than that of control group (44.4%) in acupuncture groups, respectively. Emetic rates in PC-6 (16.7%) and BL-21 (0.0%) were lower than that of control group (38.9%) in aqua-acupuncture groups, respectively. Based on these findings, it was considered that the antiemetic effects of acupuncture and aqua-puncture at BL-21 were more effective than those of PC-6 groups, and aqua-acupuncture with metoclopramide at BL-21 was the most effective among them.

• Journal of Magnetics
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• 제21권3호
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• pp.399-404
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• 2016

This paper presents a new permanent magnet (PM) assisted topology for a recently introduced brushless wound rotor synchronous machine (BL-WRSM) [1]. The BL-WRSM had a dual-inverter configuration for generating a composite magneto motive force (MMF) with a fundamental component and a subharmonic component. The subharmonic component of the MMF is used for brushless excitation of the rotor. In this paper, additional PMs were introduced on the rotor of the BL-WRSM, making it a hybrid BL-WRSM. We also discussed the flux weakening operation for the hybrid BL-WRSM. The hybrid BL-WRSM offered advantages for starting the machine and provided better performance under full-load conditions. The finite element method (FEM) was used to analyze the performance of the hybrid BL-WRSM, and we compared its performance with BL-WRSM. Finally, prototypes were built with and without the PM-assistance, and experiments were conducted to demonstrate their performance.

• Journal of Acupuncture Research
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• 제24권4호
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• pp.55-68
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• 2007

Objectives : This research was performed to investigate the effect of herbal acupuncture(Crataegus pinnatifida) at WI-SU($BL_{21}$) on weight gain, food intake, food efficiency, serum of lipid concentrations, liver function and cardiovascular risk of rats fed high fat diet for 8weeks. Methods : Experimental groups were divided into high fat diet group(Control), high fat diet and saline in $BL_{21}$ treated group(Saline), high fat diet and Crataegus pinnatifida-herbal acupuncture in $BL_{21}$ treated group(HA-1, 0.08mg/ml/g), high fat diet and Crataegus pinnatifida-herbal acupuncture in $BL_{21}$ treated group(HA-2, 0.04mg/ml/g), high fat diet and Crataegus pinnatifida-herbal acupuncture in $BL_{21}$ treated group(HA-3, 0.016mg/ml/g), Herbal acupuncture was bilaterally treated at Crataegus pinnatifida-herbal acupuncture in both $BL_{21}$ 1time (0.2cc) per 4days, total 14 times in 56days. Results : Body weight was decreased in HA-1 and food efficiency was decreased in HA-1, HA-3. Feed intake was increased in HA-3. The level of serum total cholesterol was decreased in Saline, HA-1, HA-2, HA-3. The level of serum HDL-cholesterol was increased in HA-1, but LDL-cholesterol was not significant. The level of serum triglyceride was decreased in HA-1, HA-2, HA-3. and the level of serum Total Bilirubin was decreased in HA-1. The level of serum Atherogenic index was decreased in HA-1, HA-2, HA-3. and the level of serum HTR was increased in HA-3. The evel of serum AST, ALT were increased in saline, and the level of serum r-GTP was not significant. Conclusions : Crataegus pinnatifida acupuncture in Wi-Su($BL_{21}$) can control the body weight, feed intake and feed efficiency ratio, total cholesterol, HDL-cholesterol, triglyceride, total bilirubin, and prevention cardiovascular risk. And Crataegus pinnatifida acupuncture is not injurious on liver function.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.550-553
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• 2003

$Hisx_6-GFPuv-Cyt$ c fusion protein을 E. coli 균주 JM109과 BL21 각각에서 발현시킨 결과 발현온도는 $30^{\circ}C$보다 $37^{\circ}C$에서, BL21보다 JM109에서의 발현량이 더 많았다. 그러나 JM109, $37^{\circ}C$에서 발현시킨 fusion protein의 $Ni^{2+}-IDA-agarose$ purification결과 약 45kDa 부근의 fusion protein의 density가 감소되었음을 SDS-PAGE analysis을 통해 알 수 있었다. 또한 western blotting analysis를 통해 이 impurity가 degraded fusion임을 확인 할 수 있었다. degraded fusion은 BL21 균주에서 발현시킨 fusion protein에서도 생성됨을 확인하였다. 모든 결과를 종합해 볼때 $Hisx_6-GFPuv-Cyt$ c fusion protein의 발현은 IM109, $37^{\circ}C$에서 더 많았지만, BL21, $37^{\circ}C$에서 expression시킨 fusion protein이 보다 안정하다고 판단 되어진다. 향후 fusion protein이 bioelectronic device에 적용되려면 degraded fusion protein의 생성을 줄여 activity를 유지하도록 안정한 형태로 발현되어 순수하게 분리정제 되어야 하겠다.

• 한국임상수의학회지
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• 제24권4호
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• pp.608-612
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• 2007

4년령의 중성화한 수컷 코커 스파니엘이 후지 마비와 진전 때문에 내원 하였다. 본 환축은 X-ray와 MRI를 이용하여 추간판탈출증이 합병된 척수연화증으로 진단되었다. 환축의 치료를 위하여 봉독 약침, 뜸 및 한약제의 투여를 이용하였다. 사용 혈위는 GV02-1, GV04, GV11, GV12, GV13, GV14, BL11, BL18, BL23, BL25, BL28, GB21, HT07, KI03, LI04, LU07, SI03, SI06, ST36, ST38, ST39, ST40, ST41, GB30, GB31, GB34, LIV03, SP06 및 압통점($T1{\sim}T4\;and\;T9{\sim}L1$) 이었다. 그 결과, 1차 치료 시 환축은 기립이 불가능하였으며, 사지를 움직이지 못하였다. 그러나 7차, 14차 및 21차 치료 시 점차적인 임상증상의 개선이 관찰되었다. 비록 한방수의학적 방법으로 임상 증상이 완치된 것은 아니지만, 21차 치료 후 임상증상이 일부 호전된 증례이었다.