• Biomedical Science Letters
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• v.12 no.2
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• pp.127-130
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• 2006

It is important to coordinated interaction among neurons, astrocytes and endothelial cells to maintain the function of brain. To study their regulatory mechanisms in vitro system, the co-culture system among the isolated cells from brain may be needed. However, the method for purifying brain microvascular endothelial cells (BMEC) far culture have not established yet. In this study, the proper culture methods of mice cells using two different strains, CD1 and C57BL6, to obtain the pure and plentiful endothelial cells were described. The flatted-round forms of CD1 endothelial cells grew on the collagen-IV coating plates, while the purified cells from C57 mice preferred type collagen-I dishes for their growth. Both cells displayed anti-PECAM-1 (CD31) and von Willebrand Factor immune-reactivity. These results indicated that different coating materials not only improve attachment of isolated cells but also promoting growth of cells, suggesting that this method of purifying murine Brain microvascular endothelial cells (BMEC) provides a suitable model to investigate blood-brain-barrier (BBB) properties within neurovascular unit in vitro.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2009.01a
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• pp.413-417
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• 2009

The use of scalable video coding scheme has been regarded as a promising solution for guaranteeing the quality of service of the video streaming over the Internet because it is a capable coding scheme to perform quality adaptation depending on network conditions. In this paper, we use a streaming model that transmits base layer using TCP and enhancement layers using DCCP, which try to provide transmission reliability of the BL and TCP friendliness. Unlike pervious works, the proposed algorithm performs rate adaptation based on playout buffer status. The PoB status of the client is sent back periodically to the server and serves as a network congestion indicator. Experimental results show that our scheme improves streaming quality comparing with pervious scheme in the case of not only constant/dynamic background flows but also VBR-encoded video sequence.

• Journal of the Korean institute of surface engineering
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• v.26 no.3
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• pp.109-114
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• 1993

Black magnetite film could be formed on Invar steel for shadows mask by iron electroplating in thickness of 0.46~0.63$\mu\textrm{m}$ on Invar steel and subsequent oxidation of iron by immersion in strongly alkaline soution for 30~60 minutes. blackness degreed of magnetite oxide film on Invar was C20BL90 in comparision of color chart of Dainippon Ink & Chemicals Inc. Co. and adhesion test showed excellent adhesion of oxide film on substrate. Magnetite oxide film grew up on iron in hot strongly alkaline solution according to 'Sato-Cohen' oxide film growth model.

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.104.1-104.1
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• 2003

The purpose of this study is to investigate the effect of the samples on the blood levels of glucose, glycohemoglobin(HbAlc), total cholesterol and triglyceride. The samples have been used in the treatment of a type 2 diabetic animal model (C57BlKsj db/db). The samples were administrated orally before each meal for 6 weeks. Cinnamon dose was 50mg/kg/day, 100mg/kg/day, 150mg/kg/day and 200mg/kg/day, respectively. Rhodiola rosea dose was 50mg/kg/day, 100mg/kg/day, 15-mg/kg/day and 200mg/kg/day, respectively. (omitted)

• Journal of Physiology & Pathology in Korean Medicine
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• v.19 no.1
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• pp.106-113
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• 2005

Asthma is an inflammatory disease of airways that is induced by Th2 cytokines and inhibited by Th1 cytokines. In this study we wanted to investigate the effect of SCT and BJT on eosinophilla and cytokines of BALF in a mouse model established airway inflammmation. Asthma was induced to male c57/bl6 mice. Allergen-specific antibody responses, cytokine$(IL-4,\;IL-5,\;INF-{\gamma})$, and eosinophil inflammation of the airways were investigated on the BALF and splenocyte. SCT and BJT effectively induced $INF-{\gamma}$ and inhibited IL-4, IL-5 as well as eosinophilic inflammation when SCT and BJT were administered. Total Ig E level in the BALF decreased. SCT was more effectiveness than BJT. It is considered that SCT and BJT have faborable effect on the asthma because the asthma specific series of abnormalities in respiratory system were decreased.

• Journal of Physiology & Pathology in Korean Medicine
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• v.28 no.4
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• pp.403-410
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• 2014

The purpose of this study was to examine the anti-depressive effects of OnDam-Tang with addition of Linderae Radix (ODT-L) on the animal model of depression induced by chronic immobilization stress. Depression model was made by chronic immobilization stress for 2 hours for 21 days. And we performed forced swimming test, analysis of the neurotransmitter and immunohistochemical staining, measured expression levels of serotonin in the brain. ODT-L has decreased immobilization time in forced swimming test. ODT-L has increased amount of melatonin in the brain. ODT-L has increased expression levels of serotonin in the brain. ODT-L prevented damage in the hippocampal region. ODT-L has reduced the expression level of CRF receptors in in hippocampus region. These results suggest that ODT-L may have anti-depressive effects on depression.

• Journal of TMJ Balancing Medicine
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• v.9 no.1
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• pp.4-11
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• 2019

Objectives: Although intraoral balancing appliance therapy has been used effective to several diseases, verification studies through cerebral diseases are poorly reported so far. Thus we investigated the effect of tooth-cut induced dental malocclusion against mouse model of ischemic stroke. Methods: Tooth-cut and 90 min middle cerebral artery occlusion (MCAO) were loaded to C57BL/6 male mice, and total infarct area, neurological deficit scores (NDS), histological change of hippocampal region were observed. Production levels of reactive oxygen species (ROS) and inducible nitric oxide synthase (iNOS) in cerebral tissue were also measured. Results: The longer the tooth-cut period, the greater the area of cerebral infarction caused by MCAO, and NDS began to increase as the tooth was cut, and the results were more negative when MCAO was loaded. Histological change of hippocampal cells was significant when tooth-cut was maintained for 7 days. Those damages were thought to depend on the generation of ROS and iNOS in brain tissue. Conclusions: Since tooth-cut increased total area of cerebral infarction due to MCAO in mice, it is able to be confirmed that anomaly of the temporomandibular occlusion can affect neurological diseases.

• Journal of Acupuncture Research
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• v.31 no.1
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• pp.139-148
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• 2014

Objectives : The aim of this study was to review the effect of herbal medicine on hair growth in animal models reported in Korean domestic journals. Methods : Korean core databases were searched with terms alopecia and hair, and animal study reports on hair growth with herbal medicine were reviewed. Animal model, intervention, and hair growth measure data were extracted. Results : A total of 28 reports were reviewed. C57BL/6 mice with depilation was the most frequent animal models used. Polygoni multiflori radix, Cnidii rhizoma and Biotae Folium were frequently used herbal medicine. Positive effects were reported. Conclusions : Herbal medicines may be promising resource for pharmacopuncture materials for positive effects on hair growth.

• Journal of Physiology & Pathology in Korean Medicine
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• v.20 no.5
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• pp.1285-1289
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• 2006

Hominis placenta (HP) has been used as an agent for promoting physiological function in traditional asian medicine. The present study was peformed to investigate whether HP acupuncture treatment in an experimental tumor mice model inhibit tumor growth through immunomodulatory effects. Mice were inoculated subcutaneously with colon26-L5 cells on the back. Three days after tumor inoculation, HP herbal acupuncture treatment was conducted on BL18 acupoint every other day for three weeks. HP Herbal acupuncture treatment significantly suppressed the primary tumor growth and prolonged survival rate. To evaluate immunomodulatory effect of HP acupuncture, splenocytes proliferation assay, fluorescence-activated cell sorting (FACS) and ELISA for IFN- ${\gamma}$, and IL-4 cytokine level. HP herbal acupuncture enhanced the mitogenic activity of Balb/c whole splenocytes induced by various mitogenic stimuli and increased immune cell population such as T cell, B cell, Th cell, Tc cell and Macrophages. HP herbal acupuncture caused a marked increase of production of Th1 cytokine (IFN- ${\gamma}$ ,) and decrease of production of Th2 cytokine (IL-4). These results indicated that HP herbal acupuncture suppresses tumor growth through a mechanism leading to a Th1 dominant immune state.

• Toxicology and Environmental Health Sciences
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• v.9 no.5
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• pp.279-282
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• 2017

Lactic acid bacteria (LAB) provide numerous beneficial effects on the host body, especially on the intestine. Two LAB strains isolated from Kimchi, Leuconostoc mesenteroides and Lactobacillus sakei, were studied for its anti-inflammatory activity in acidinduced acute colitis in mice. To induce acute colitis in model mice (C57BL/6), 3% of dextran sulfate sodium treatment was treated for 7 days. Assessment of necropsy and histopathology analysis showed that oral supplementation of both L. mesenteroides and L. sakei ameliorated the symptoms of acute colitis. Moreover, the mixture of L. meseneroides and L. sakei showed synergistic effect on colitis. The results suggest that the formulation of L. mesenteroides and L. sakei mixture could be used as an oral supplementation to decrease the inflammatory harmful environment associated with colitis.