• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2003년도 제10차 국제학술회의 및 추계정기 학술발표회
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• pp.57-57
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• 2003

The plant growth promoting rhizobacteria (PGPR), Pseudomonas sp. (A3) and Bacillus sp. (AT33) were isolated from the rhizosphere of Amaranthus blitum collected from soil contaminated with chromium. Bothbacterial strains quantitatively reduced hexavalent chromium to trivalent chromium. Pseudomonas sp. broughter greater conversion of Cr6+ in the medium (100%) as compared to Bacillus sp.(62%). Phaseolus mungo seeds inoculated with Pseudomonas sp. or Bacillus sp. were grown under different concentration of chromium. The monitoredparameters included elongation of shoot and root, fresh weight, dry weight and concentration of chromium in the shoot and root systems. As compared to non inoculated seedlings those inoculated with A3 and AT33 exhibited better growth.

• 한국균학회지
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• 제49권4호
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• pp.525-530
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• 2021

The cultivation in sawdust media, mushroom productivity, and fruiting body characteristics of Lentinula edodes strains NIFoS 2778 and NIFoS 3363 were compared according to the inoculation conditions. The cultivation period was 5% shorter when liquid spawn was used. Fruiting bodies were induced after 113 days of incubation on media inoculated with liquid spawn, and the cultivation period was 119 days on media inoculated with solid spawn. Mushroom productivity of NIFoS 2778 was the highest (661.4 g) when 36 mL of liquid spawn was used. For NIFoS 3363, mushroom production was higher under liquid inoculation conditions when the same amount of liquid and solid spawns were used. The mushroom characteristics of the two strains were not significantly different, except for gill width and stipe diameter.

• International Journal of Industrial Entomology and Biomaterials
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• 제44권2호
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• pp.29-36
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• 2022

Over the past decades, silk sericin (SS) received increasing attention in the academic and industrial fields. In nature, SS acts as a glue that holds the two strands of silk fibrils together. However, recent works suggest that SS might have a more diverse role during the silk spinning process, such as stabilizing the SF in the silk gland. On the other hand, the sericulture industry has been trying to find novel applications for SS discarded from the silk fabric manufacturing process. Recovery and refining of SS would be the first step of the recycling of SS. Using a proper solvent SS could be shaped into various forms, such as spherical beads, microparticles, fibers, and films. Moreover, the applicability of these SS materials has been investigated in various fields such as cosmetics, templates for nanomaterials, drug delivery, heavy metal adsorption, and enzyme immobilization.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2002년도 춘계학술대회
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• pp.177-177
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• 2002

• Journal of Biosystems Engineering
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• 제27권3호
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• pp.185-194
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• 2002

The objective of this study was to analyze a stability of sideways overturning of a forwarder developed by the Forestry Research Institute. The stability analysis was conducted using a multibody dynamic analysis program. VisualNastran Desktop. A solid model of the forwarder was constructed and its physical properties such as mass, mass center and mass moment of inertia were determined on 3D CAD modeler of the Solid Edge 8.0. 3D simulations of sideways overturning of the forwarder were also performed on the Visual Nastran using the solid model when it traveled across the slope and traversed over an up-hill side obstacle. Stability comparison between a bogie-wheeled and a 6-wheeled forwarders was also made and found that the bogie-wheeled forwarder was more stable than the 6-wheeled one on slopes. The safety speeds of the forwarder predicted by the simulation under various conditions can be used as a guideline for safe operations of forwarders in mountainous area. The technique of using a solid model for the sability analysis can also be applied successfully to other vehicles like agricultural tractors, loaders and construction equipments.

• BMB Reports
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• 제28권1호
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• pp.57-61
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• 1995

The expression of a recombinant human lactoferrin is reported in mouse HC11 mammary epithelial cells. Expression of human lactoferrin (hLF) was achieved by placing its cDNA under the control of the bovine ${\beta}$-casein gene. To improve the hLF expression level in a cell culture system, two artificial introns were also introduced to construct expression vectors. One intron was a hybrid-splice signal consisting of bovine ${\beta}$-casein intron 1 and rabbit ${\beta}$-globin intron II. The other intron was a DNA fragment spanning intron 8 of the bovine ${\beta}$-casein gene. The hybrid intron moderately elevated hLF expression, whereas intron 8 alone did not express any detectable amount of hLF as judged by Northem and Western blot analyses. When the two introns were used together they contributed to a synergistic elevation of hLF expression. These data indicate that artificial introns on both sides of the hLF cDNA were necessary to increase expression of cDNA.

• Asian-Australasian Journal of Animal Sciences
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• 제31권11호
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• pp.1729-1737
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• 2018

Objective: The aim of the current study is to investigate the relationship between prohibitin (PHB), capping actin protein of muscle Z-line beta subunit (CAPZB), and tektin-2 (TEKT2) and sperm motility in Murrah buffalo. Methods: We collected the high-motility and low-motility semen samples, testis, ovary, muscle, kidney, liver, brain and pituitary from Murrah buffalo, and analysed the expression of PHB, CAPZB, and TEKT2 in mRNA (message RNA) and protein level. Results: Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) result showed that the expression of PHB was higher and CAPZB, TEKT2 were specifically expressed in testis as compared to the other 6 tissues, and that in testis, the expression of TEKT2 was higher than that of CAPZB and PHB. Immunohistochemistry test revealed that all three genes were located on the convoluted seminiferous tubule and enriched in spermatogenic cells. Both qRT-PCR and Western Blot results showed that the expression levels of PHB, CAPZB, and TEKT2 were significantly lower in the low-motility semen group compared to the high-motility semen group (p<0.05). Conclusion: The expression levels of PHB, CAPZB, and TEKT2 in Murrah buffalo sperm have a high positive correlation with sperm motility. And the three genes may be potential molecular markers for the decline of buffalo sperm motility.

• 한국축산식품학회지
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• 제36권1호
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• pp.114-121
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• 2016

This study was performed to improve the techniques used for tenderizing red meat as elderly food. Beef meat was immersed in liposome encapsulated enzyme solution and the effect of protease encapsulation on the beef properties was analyzed. The protease encapsulation properties were analyzed according to the size distribution and enzymatic activity. After enzyme reaction on the beef, the chemical properties of the meat such as pH, water holding capacity, shear rate, lipid oxidation and total volatile basic nitrogen (TVB-N) were analyzed. The pH of the beef increased during the reaction and coating protease (CP) was higher than non-coating protease (NCP). Total color differences were increased remarkably after 36 h and generally, the difference in CP was relatively lower than in NCP. WHC was significantly decreased within 24 h, and no effect from the protease coating was observed. Protease activity was significantly increased within 48 h and no differences in the enzyme coating were observed. The TVB-N value of NCP was increased within 24 h while CP was sustained for up to 36 h. The TVB-N value of protease treated meat increased after 36 h and no effect from the protease coating was detected. Consequently, liposome encapsulated protease was found to have similar properties as non-coated protease. Application of liposome seems to be an interesting option for injecting various functional materials without changing the properties of meat.

• 한국자원식물학회지
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• 제20권4호
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• pp.281-291
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• 2007

본 연구는 경북 영양군 검마산(해발 1,0l7m, 북위 $36^{\circ}\;42^{'}\;30.0^{"}{\sim}36^{\circ}\;45^{'}\; 30.1^{"}$, 동경 $129^{\circ}\;12^{'}\;30.0^{"}{\sim}129^{\circ}\;18^{'}\; 00.0^{"}E$)의 관속식물 분포를 파악하기 위하여 수행되었다. 2004년 4월부터 2006년 10월까지 11회에 걸쳐 채집된 관속식물은 총 91과 318속 424종 3아종 73변종 13품종의 총 513종류로 정리되었으며, 이들을 유용성에 따라 구분하면 식용 243종류, 약용 307종류, 공업용 122종류, 관상용 85종류, 용도가 밝혀지지 않은 식물 77종류이었다. 특기할만한 식물로는 한국특산식물이 9종류, 환경부지정 법적 보호식물 4종류, 귀화식물이 21종류로서 귀화율은 4.1%, 도시화지수는 7.5%로 나타났다.

• Mycobiology
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• 제43권3호
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• pp.319-326
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• 2015

Fomes fomentarius is a fungus of the Polyporaceae family and is used in traditional oriental therapies. Although the anti-inflammatory activities of this species have been previously reported, the identity of the bioactive compounds responsible for this activity remains unknown. Here, we investigated whether methyl 9-oxo-(10E,12E)-octadecadienoate (FF-8) purified from F. fomentarius exerts anti-inflammatory activity in murine macrophages stimulated with lipopolysaccharide (LPS). FF-8 suppressed secretion of nitric oxide (NO) and prostaglandin $E_2$ through downregulation of inducible NO synthase and cyclooxygenase-2 expression induced by LPS. In addition, pretreatment of cells with FF-8 led to a reduction in levels of secreted inflammatory cytokines such as tumor necrosis factor-${\alpha}$ and interleukin-6 in macrophages stimulated with LPS. Conversely, FF-8 did not affect nuclear factor ${\kappa}B$, p38, c-Jun NH2-terminal kinase, and extracellular signal-regulated kinase pathways. Instead, FF-8 specifically interfered with signal transducer and activator of transcription 3 (STAT3) phosphorylation induced by LPS. Collectively, this study demonstrated that FF-8 purified from F. fomentarius suppresses inflammatory responses in macrophages stimulated with LPS by inhibiting STAT3 activation. Further studies will be required to elucidate the anti-inflammatory effect of FF-8 in vivo.