• Mycobiology
• /
• 제50권5호
• /
• pp.302-316
• /
• 2022

Many Apiospora species have been isolated from bamboo plants - to date, 34 bambusicolous Apiospora species have been recorded. They are known as saprophytes, endophytes, and plant pathogens. In this study, 242 bambusicolous Apiospora were isolated from various bamboo materials (branches, culms, leaves, roots, and shoots) and examined using DNA sequence similarity based on the internal transcribed spacer, 28S large subunit ribosomal RNA gene, translation elongation factor 1-alpha, and beta-tubulin regions. Nine Apiospora species (Ap. arundinis, Ap. camelliae-sinensis, Ap. hysterina, Ap. lageniformis sp. nov., Ap. paraphaeosperma, Ap. pseudohyphopodii sp. nov., Ap. rasikravindrae, Ap. saccharicola, and Ap. sargassi) were identified via molecular analysis. Moreover, the highest diversity of Apiospora was found in culms, and the most abundant species was Ap. arundinis. Among the nine Apiospora species, two (Ap. hysterina and Ap. paraphaeosperma) were unrecorded in Korea, and the other two species (Ap. lageniformis sp. nov. and Ap. pseudohyphopodii sp. nov.) were potentially novel species. Here, we describe the diversity of bambusicolous Apiospora species in bamboo organs, construct a multi-locus phylogenetic tree, and delineate morphological features of new bambusicolous Apiospora in Korea.

• Journal of Microbiology and Biotechnology
• /
• 제29권1호
• /
• pp.91-104
• /
• 2019

The taxonomic and functional characteristics of bacterial communities in the pre-chlorinated rapid filters and ozonated biological activated carbon (BAC) filters were compared using Illumina MiSeq sequencing of the 16S rRNA gene and community-level physiological profiling (CLPP) based on sole-carbon-source utilization patterns. Both the rapid filters and BAC filters were dominated by Rhizobiales within ${\alpha}-proteobacteria$, but other abundant orders and genera were significantly different in both types of filter. Firmicutes were abundant only in the intermediate chlorinated rapid filter, while Acidobacteria were abundant only in the BAC filters. Bacterial communities in the rapid filter showed high utilization of carbohydrates, while those in the BAC filters showed high utilization of polymers and carboxylic acids. These different characteristics of the bacterial communities could be related to the different substrates in the influents, filling materials, and residual disinfectants. Chlorination and ozonation inactivated the existing bacteria in the influent and formed different bacterial communities, which could be resistant to the oxidants and effectively utilize different substrates produced by the oxidant, including Phreatobacter in the rapid filters and Hyphomicrobium in the BAC filters. Bradyrhizobium and Leptothrix, which could utilize compounds adsorbed on the GAC, were abundant in the BAC filters. Ozonation increased taxonomic diversity but decreased functional diversity of the bacterial communities in the BAC filters. This study provides some new insights into the effects of oxidation processes and filling materials on the bacterial community structure in the biological filters of drinking water treatment plants.

• Mycobiology
• /
• 제51권5호
• /
• pp.300-312
• /
• 2023

Hydnum is a genus of ectomycorrhizal fungi belonging to the Hydnaceae family. It is widely distributed across different regions of the world, including North America, Europe, and Asia; however, some of them showed disjunct distributions. In recent years, with the integration of molecular techniques, the taxonomy and classification of Hydnum have undergone several revisions and advancements. However, these changes have not yet been applied in the Republic of Korea. In this study, we conducted an integrated analysis combining the morphological and molecular analyses of 30 specimens collected over a period of approximately 10 years in the Republic of Korea. For molecular analysis, the sequence data of the internal transcribed spacer (ITS) region, the large subunit of nuclear ribosomal RNA gene (nrLSU), and a portion of translation elongation factor 1-a (TEF1) were employed as molecular markers. Through this study, we identified eight species that had previously not been reported to occur in the Republic of Korea, including one new species, Hydnum paucispinum. A taxonomic key and detailed descriptions of the eight Hydnum species are provided in this study.

• Journal of Forest and Environmental Science
• /
• 제26권1호
• /
• pp.1-7
• /
• 2010

Species diversity is one of the most important specifications of biological societies. Diversity of organisms, measurement of variety and examination of those hypotheses that are about reasons of diversity are such as affairs that have been desired by the ecologists for a long time. In this research, diversity of plant species in forest region, numbers of 60 sample plots in 256.00 square meters have been considered in random - systematic inventory was considered. In each sample plot, four micro-plots in 2.25 square meters in order to study on herbal cover, were executed that totally 240 micro-plots were considered. At each plot six diversity indices in relation to physiographic factors (slope, geographical aspect and altitude from the sea level) were studied. The results indicate that species diversity is more in the northern direction and also species diversity in slops less than 30% has the most amounts. Factor of altitude from the sea level did not have meaningful relation with species diversity. Through study on correlation of the numbers of species in sample plots with indices and also process and role of indices in different processors of analysis, Simpson's reciprocal index was suggested as suitable index in this type of studies.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2017년도 9th Asian Crop Science Association conference
• /
• pp.161-161
• /
• 2017

Seed endophytes are very remarkable groups of bacteria for their unique abilities of being vertically transmitted and conserved. As plants attain hybrid vigor and heterosis in the process of crossbreeding, this might also lead to the changes in the community structure and diversity of plant endophytes in the hybrid plants ultimately affecting the endophytes of the seeds. It would be interesting to characterize how seed endophyte composition change over time. The objective of this study is to gain insights into the influence of natural crossbreeding and parental lineage in the seed bacterial endophytic communities of two pure inbred lines exploring contributions of the two most important sources of plant endophytes - colonization from external sources and vertical transmission via seeds. Total genomic DNA was isolated from rice seeds and bacterial DNA was selectively amplified by PCR. The diversity of endophytic bacteria was studied through Terminal-Restriction Fragment Length Polymorphism (T-RFLP) analysis. Diversity between the original parents and the pure inbred line may show significant differences in terms of richness, evenness and diversity indices. Heat maps reveal astonishing contributions of both or either parents (IR29 ${\times}$ Pokkali and AT401 ${\times}$ IR31868) in the shaping of the bacterial seed endophytes of the hybrid, FL478 and IC32, respectively. Most of the T-RFs of the subsequent pure inbred line could be traced to any or both of the parents. Comparison of common and genotype-specific T-RFs of parents and their offspring reveals that majority of the T-RFs are shared suggesting higher transmission of bacterial communities common to both parents. The parents influence the bacterial community of their offspring. Unique T-RFs of the offspring also suggest external sources of colonization particularly as the seeds are cultivated in different ecogeographical locations. This study showed that host parental lines contributed greatly in the shaping of bacterial seed endophytes of their offspring. It also revealed transmission and potential conservation of core seed bacterial endophytes that generally become the dominant microbiota in the succeeding generations of plant hosts.

• 한국응용곤충학회지
• /
• 제60권1호
• /
• pp.49-61
• /
• 2021

과수에서 초기 해충종합관리(IPM) 모형은 응애류 종합관리를 기반으로 수립되었다고 할 수 있다. 근간은 기존 방제체계인 화학적 방제와 호환적인 포식성 이리응애 천적을 접목한 생물적 방제가 작동하는 것에 있었다. 이후로 생태계 종다양성과 해충 개체군 변동과의 상호작용에 대한 가설과 기작은 과수 생태계에서 응애류 중심의 해충관리에 대한 더 넓은 이해의 폭을 제공하였다. 생태계의 원리를 바탕으로 환경의 개변 또는 변경을 통한 생물적 방제와 해충관리는 농생태공학적 개념으로 발전하고 있다. 특히 응애류 해충의 관리에 있어서는 재배환경의 변화에 따라 관련된 천적의 다양성이 역동적으로 변동되어 작동하고 있으며, 식식성 응애류의 종 구성도 과원관리 환경에 따라 변화를 보이고 있다. 본 원고에서는 사과 IPM의 근간이 되었던 응애류 생물적 방제를 고찰하면서 생태공학적 측면에서 종다양성과 해충관리, 포식성 응애류 종다양성과 응애류 관리, 환경과 응애류의 종 구성 변동 원리에 대하여 기존 사례연구를 바탕으로 재조명하고, 향후 우리나라에서 사과원 응애류의 생물적 방제 전략을 제시하고자 한다.

• 한국환경과학회지
• /
• 제14권12호
• /
• pp.1119-1127
• /
• 2005

The management of data on biological diversity is presently complex and confusing. This study was initiated to construct a database so that such data could be stored in a data management, and analysis instrument to correct the problems inherent in the current incoherent storage methods. MySQL was used in DBMS(DataBase Management System), and the program was basically produced using Java technology Also, the program was developed so people could adapt to the requirements that are changing every minute. We hope this was accomplished by modifying easily and quickly the advanced programming technology and patterns. To this end, an effective and flexible database schema was devised to store and analyze diversity databases. Even users with no knowledge of databases should be able to access this management instrument and easily manage the database through the World Wide Web. On a basis of databases stored in this manner, it could become routinely used for various databases using this analysis instrument supplied on the World Wide Web. Supplying the derived results by using a simple table and making results visible using simple charts, researchers could easily adapt these methods to various data analyses. As the diversity data was stored in a database, not in a general file, this study makes the precise, error-free and high -quality storage in a consistent manner. The methods proposed here should also minimize the errors that might appear in each data search, data movement, or data conversion by supplying management instrumentation on the Web. Also, this study was to deduce the various results to the level we required and execute the comparative analysis without the lengthy time necessary to supply the analytical instrument with similar results as provided by various other methods of analysis. The results of this research may be summerized as follows: 1)This study suggests methods of storage by giving consistency to diversity data. 2)This study prepared a suggested foundation for comparative analysis of various data. 3)It may suggest further research, which could lead to more and better standardization of diversity data and to better methods for predicting changes in species diversity.

• Animal Systematics, Evolution and Diversity
• /
• 제36권2호
• /
• pp.154-158
• /
• 2020

The Asian crested ibis Nipponia nippon is one of the world's most endangered species. Except for the Sanxii population from China, it is known that all of the crested ibis populations from East Asia have been extinguished. In these days, most of them are being inbred as captive populations in China, South Korea, and Japan, which caused their low expected genetic diversity. Microsatellite markers are well known as a suitable DNA marker for exploring genetic diversity among captive populations of a variety of endangered species. In the present study, ten microsatellite markers were developed for the captive populations of the South Korean crested ibis, which were employed to examine the level of genetic diversity with the two founders from Sanxii, China and the 70 descendants of them. As a result, the mean number of gene diversity, observed heterozygosity, and expected heterozygosity of the captive population were 0.70, 0.84, and 0.70 respectively. It revealed that the captive population of South Korea is as genetically more stable than we expected. In addition, the principal coordinates analysis and genetic structure analyses showed that the captive population of N. nippon can be divided into the two different genetic groups. The developed microsatellite markers here could be helpful for crested ibis conservation in East Asian countries such as China and Japan as well as South Korea.

• 한국환경복원기술학회지
• /
• 제23권6호
• /
• pp.167-185
• /
• 2020

The purpose of this study is to prepare a plan for improving the bird habitat environment of Linear Park. To this end, after grasping the status of bird habitats on the Gyeongchun Line Forest Road, a representative linear park in Seoul, the habitat environment was evaluated and the correlation with the bird habitat was analyzed to derive a plan to improve the habitat environment. The results for correlation between diversity of birds and habitat environment were as follows. For the habitat environment inside the park, the order of positive correlation was in the order of park area (0.92), number of insect species (0.87), green area ratio (0.77), average width of linear park (0.74), Biotope area ratio (0.73), Immigration planted species (0.57). Also, for habitat environment outside the park, the bird diversity was influenced in the order of area outside the park (0.88), green area ratio of the park (0.76). Thus, in order to enhance the diversity of birds found in the park, the bird habitat environment inside the park needs to expand the park area, secure insect diversity, enhance green area ratio and ecological area ratio, expand the width of linear park, and lower the impermeable layer. For the bird habitat environment outside the park, wider area, green area ratio, and forest area of the park influenced on better bird habitation while lower ratio of road space and building-to-land ratio influenced on higher bird diversity. It is necessary to create an environment inhabitable for various species of birds and to make a healthy and pleasant city urban system for co-existence of human and living creatures.

• 한국해양바이오학회지
• /
• 제16권1호
• /
• pp.1-18
• /
• 2024

Coastal dunes must be conserved. Their native halophytes support coastal geography while their symbiotic microorganisms help vegetation thrive. The Goraebul coast has the largest, well-conserved dune system on the East Sea of the Korean Peninsula due to a climax mixed halophyte (C. soldanella, C. kobomugi, and E. mollis) vegetation support. This study identified rhizobacteria and their diversity in mixed halophyte communities unique to Goraebul. Five phyla, 12 genera, and 21 species were identified based on 16S rDNA sequences from 65 isolates. The phylum Bacillota, class Bacillota, order Bacillales, and family Bacillaceae were identified, with Bacillus as the dominant genus (46.15%). The richness and Shannon's diversity were higher at the species than at the genus level due to the dominance of Bacillus; however, various Bacillus species (7) were identified. Therefore, the climax mixed vegetation adapted to the Goraebul coast may exert natural selection pressure in favor of the common characteristics of Bacillus. However, despite this advantage, the Shannon equitability (0.86), Simpson (0.08), and Shannon diversity (2.79) indexes indicate a stable rhizosphere cluster and the climax mixed vegetation is affected by symbiotic relationships between healthy rhizosphere microbiota.