• 제목/요약/키워드: BIOENGINEERING AND TECHNOLOGY

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송이버섯 추출액의 항균력 확인과 저염젓갈 생산에 적용 가능성 (Determination of Antibacterial Activity from Tricholoma matsutake Extract and Its Application to Low Salted Jeot-gal)

  • 김진성;박재범;장승원;권덕호;장미희;이미옥;하석진
    • KSBB Journal
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    • 제30권5호
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    • pp.253-256
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    • 2015
  • 송이버섯 추출액의 박테리아에 대한 항균력을 측정하기 위하여 최소저해농도를 확인한 결과 젓갈 제조시 빈번하게 발생하는 부패균, 어병원인 균등 9가지 박테리아에 대해 성장이 저해되는 것을 확인하였다. 확인된 송이버섯 추출액의 항균력을 저염 젓갈의 제조 공정에 적용하기 위해 식품보존제인 사과산나트륨과 아질산나트륨을 함께 첨가하여 S. aureus에 대한 최소저해농도를 확인하였다. 그 결과 사과산나트륨과 아질산나트륨의 최소저해농도가 각각 0.25 g/L와 0.025 g/L로 크게 낮아지는 것을 확인할 수 있었다. 본 연구를 통해 항균력을 갖는 송이버섯 추출액을 첨가함으로써 일반적으로 첨가되는 식품보존제의 농도를 크게 낮출 수 있었을 뿐 아니라 송이버섯의 다양한 기능성이 추가된 고급화된 저염 젓갈을 개발할 수 있을 것으로 예상된다.

Scenedesmus sp.로부터 Taguchi 법을 이용한 지방추출의 최적화 (Optimization of Lipid Extraction from Scenedesmus sp. Using Taguchi Approach)

  • 김나영;오성호;최운용;이현용;이신영
    • KSBB Journal
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    • 제25권4호
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    • pp.371-378
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    • 2010
  • For the biodiesel fuel production from microalgae, the lipid from wet and dry samples of green algae Scenedesmus sp. was extracted by using various solvents and pre-treatment methods. Extraction yield of the lyophilized sample was better than that of dry sample. Chloroform/methanol (2:1, v/v) and ultrasonication or homogenization method were also selected as the most effective solvent and pre-treatment methods for lipid extraction, respectively. Under these constraint conditions, optimization experiment of lipid extraction was investigated by Taguchi approach using orthogonal matrix $L_9$ ($3^4$) method. The optimum extraction conditions of lipid extraction was obtained at pre-treatment of homogenization, extraction time of 5 hour, temperature of $35^{\circ}C$, and solvent ratio of 1:20 (w/v). Yield of extraction at optimized condition was 20.55% and it was 96% of total lipid content (21.38%) of Scenedesmus sp.

Scenedesmus sp.로 부터의 지방 추출, 정제 및 특성 (Extraction, Purification and Property of the lipid from Scenedesmus sp.)

  • 김나영;오성호;최운용;이현용;이신영
    • KSBB Journal
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    • 제25권4호
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    • pp.363-370
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    • 2010
  • Lipid from Scenedesmus sp. was extracted, fractionated and purified by silicic acid column chromatography. Total lipid content of extract was $21.38{\pm}1.03$ wt%, and triacylglycerol, an index lipid for biodiesel production was detected. Ten species of $C_{16}{\sim}C_{22}$ with saturated and unsaturated fatty acid were identified showing the very adequate fatty acid profiles for biodiesel production with a good flow property at low temperature. The fractions of glycolipid, neutral and phospholipid were 52.64, 28.10 and 19.26% of total lipid, respectively. The triacylglycerol of 12.63% and chlorophyll a of 49.47% was fractioned using stepwise elution of n-hexane-diethyl ether (95:5, v/v) solvent. The high content of chlorophyll was considered as a potential source of value-added co-product.

Two-Step Oxidation of Refractory Gold Concentrates with Different Microbial Communities

  • Wang, Guo-hua;Xie, Jian-ping;Li, Shou-peng;Guo, Yu-jie;Pan, Ying;Wu, Haiyan;Liu, Xin-xing
    • Journal of Microbiology and Biotechnology
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    • 제26권11호
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    • pp.1871-1880
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    • 2016
  • Bio-oxidation is an effective technology for treatment of refractory gold concentrates. However, the unsatisfactory oxidation rate and long residence time, which cause a lower cyanide leaching rate and gold recovery, are key factors that restrict the application of traditional bio-oxidation technology. In this study, the oxidation rate of refractory gold concentrates and the adaption of microorganisms were analyzed to evaluate a newly developed two-step pretreatment process, which includes a high temperature chemical oxidation step and a subsequent bio-oxidation step. The oxidation rate and recovery rate of gold were improved significantly after the two-step process. The results showed that the highest oxidation rate of sulfide sulfur could reach to 99.01 % with an extreme thermophile microbial community when the pulp density was 5%. Accordingly, the recovery rate of gold was elevated to 92.51%. Meanwhile, the results revealed that moderate thermophiles performed better than acidophilic mesophiles and extreme thermophiles, whose oxidation rates declined drastically when the pulp density was increased to 10% and 15%. The oxidation rates of sulfide sulfur with moderate thermophiles were 93.94% and 65.73% when the pulp density was increased to 10% and 15%, respectively. All these results indicated that the two-step pretreatment increased the oxidation rate of refractory gold concentrates and is a potential technology to pretreat the refractory sample. Meanwhile, owing to the sensitivity of the microbial community under different pulp density levels, the optimization of microbial community in bio-oxidation is necessary in industry.

Detection of Heparin in the Salivary Gland and Midgut of Aedes togoi

  • Ha, Young-Ran;Oh, So-Ra;Seo, Eun-Seok;Kim, Bo-Heum;Lee, Dong-Kyu;Lee, Sang-Joon
    • Parasites, Hosts and Diseases
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    • 제52권2호
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    • pp.183-188
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    • 2014
  • Mosquitoes secrete saliva that contains biological substances, including anticoagulants that counteract a host's hemostatic response and prevent blood clotting during blood feeding. This study aimed to detect heparin, an anticoagulant in Aedes togoi using an immunohistochemical detection method, in the salivary canal, salivary gland, and midgut of male and female mosquitoes. Comparisons showed that female mosquitoes contained higher concentrations of heparin than male mosquitoes. On average, the level of heparin was higher in blood-fed female mosquitoes than in non-blood-fed female mosquitoes. Heparin concentrations were higher in the midgut than in the salivary gland. This indicates presence of heparin in tissues of A. togoi.

Monitoring of Degradation Process of Commercial ME Tapes under High Humidity Environment by AC Impedance Techniques

  • Take, Seisho;Shimanuki, Akiko;Itoi, Yasuhiko;Okuyama, Masaru
    • Corrosion Science and Technology
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    • 제3권5호
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    • pp.194-197
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    • 2004
  • The corrosion resistance of several kind of ME (Metal Evaporated) tape has been investigated both in mild sulfuric acid solution and NaCl solution by electrochemical impedance spectroscopy. It was found that the degradation of ME tapes was accelerated with increasing the concentration of sulfuric acid. There was no significant change in corrosion resistance when the concentration of NaCl was under 3.5 wt%. However, the impedance value decreased when the concentration of NaCl was up to 10 wt%. The degradation of backside of ME tapes was also investigated by AC impedance measurements. The results showed that the impedance behavior of backside plastic film changed with the concentration of sulfuric acid even at the beginning of immersion, implying the changing of the permeability for the backside of ME tapes. It was also found that the corrosion resistance of DVC (Digital Video Cassette) ME tape was better that that of Hi-8mm ME tapes in sulfuric acid solutions. Also, the backside of DVC ME tape showed better water resistance than that of Hi8 ME tapes.

SIFamide and SIFamide Receptor Define a Novel Neuropeptide Signaling to Promote Sleep in Drosophila

  • Park, Sangjin;Sonn, Jun Young;Oh, Yangkyun;Lim, Chunghun;Choe, Joonho
    • Molecules and Cells
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    • 제37권4호
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    • pp.295-301
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    • 2014
  • SIFamide receptor (SIFR) is a Drosophila G protein-coupled receptor for the neuropeptide SIFamide (SIFa). Although the sequence and spatial expression of SIFa are evolutionarily conserved among insect species, the physiological function of SIFa/SIFR signaling remains elusive. Here, we provide genetic evidence that SIFa and SIFR promote sleep in Drosophila. Either genetic ablation of SIFa-expressing neurons in the pars intercerebralis (PI) or pan-neuronal depletion of SIFa expression shortened baseline sleep and reduced sleep-bout length, suggesting that it caused sleep fragmentation. Consistently, RNA interference-mediated knockdown of SIFR expression caused short sleep phenotypes as observed in SIFa-ablated or depleted flies. Using a panel of neuron-specific Gal4 drivers, we further mapped SIFR effects to subsets of PI neurons. Taken together, these results reveal a novel physiological role of the neuropeptide SIFa/SIFR pathway to regulate sleep through sleep-promoting neural circuits in the PI of adult fly brains.

Draft Genome Sequence of the White-Rot Fungus Schizophyllum Commune IUM1114-SS01

  • Kim, Da-Woon;Nam, Junhyeok;Nguyen, Ha Thi Kim;Lee, Jiwon;Choi, Yongjun;Choi, Jaehyuk
    • Mycobiology
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    • 제49권1호
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    • pp.86-88
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    • 2021
  • The monokaryotic strain, Schizophyllum commune strain IUM1114-SS01, was generated from a basidiospore of dikaryotic parental strain IUM1114. It even showed the decolorizing activities for several textile dyes much better than its parental strain. Based on the results of a single-molecule real-time sequencing technology, we present the draft genome of S. commune IUM1114-SS01, comprising 41.1 Mb with GC contents of the genome were 57.44%. Among 13,380 protein-coding genes, 534 genes are carbon hydrate-active enzyme coding genes.