• Title/Summary/Keyword: BIOENGINEERING AND TECHNOLOGY

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Determination of Antibacterial Activity from Tricholoma matsutake Extract and Its Application to Low Salted Jeot-gal (송이버섯 추출액의 항균력 확인과 저염젓갈 생산에 적용 가능성)

  • Kim, Jin-Seong;Park, Jae-Bum;Jang, Seung-Won;Kwon, Deok-Ho;Jang, Mi-Hee;Lee, Mi-Ok;Ha, Suk-Jin
    • KSBB Journal
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    • v.30 no.5
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    • pp.253-256
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    • 2015
  • The antibacterial activity from Tricholoma matsutake extracts were confirmed by minimum inhibitory concentration (MIC) test against various bacteria. Tricholoma matsutake extracts was applied for manufacturing low salted Jeot-gal with sodium DL-malate and sodium nitrite as food preservatives. Due to antibacterial activity from Tricholoma matsutake extracts, MIC of sodium DL-malate and sodium nitrite were significantly reduced to 0.025 g/L and 0.25 g/L, respectively. As a result of this study, a premium low salted Jeot-gal can be developed with low concentration of food preservatives by adding Tricholoma matsutake extracts.

Optimization of Lipid Extraction from Scenedesmus sp. Using Taguchi Approach (Scenedesmus sp.로부터 Taguchi 법을 이용한 지방추출의 최적화)

  • Kim, Na-Young;Oh, Sung-Ho;Choi, Woon-Yong;Lee, Hyeon-Yong;Lee, Shin-Young
    • KSBB Journal
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    • v.25 no.4
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    • pp.371-378
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    • 2010
  • For the biodiesel fuel production from microalgae, the lipid from wet and dry samples of green algae Scenedesmus sp. was extracted by using various solvents and pre-treatment methods. Extraction yield of the lyophilized sample was better than that of dry sample. Chloroform/methanol (2:1, v/v) and ultrasonication or homogenization method were also selected as the most effective solvent and pre-treatment methods for lipid extraction, respectively. Under these constraint conditions, optimization experiment of lipid extraction was investigated by Taguchi approach using orthogonal matrix $L_9$ ($3^4$) method. The optimum extraction conditions of lipid extraction was obtained at pre-treatment of homogenization, extraction time of 5 hour, temperature of $35^{\circ}C$, and solvent ratio of 1:20 (w/v). Yield of extraction at optimized condition was 20.55% and it was 96% of total lipid content (21.38%) of Scenedesmus sp.

Extraction, Purification and Property of the lipid from Scenedesmus sp. (Scenedesmus sp.로 부터의 지방 추출, 정제 및 특성)

  • Kim, Na-Young;Oh, Sung-Ho;Choi, Woon-Yong;Lee, Hyeon-Yong;Lee, Shin-Young
    • KSBB Journal
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    • v.25 no.4
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    • pp.363-370
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    • 2010
  • Lipid from Scenedesmus sp. was extracted, fractionated and purified by silicic acid column chromatography. Total lipid content of extract was $21.38{\pm}1.03$ wt%, and triacylglycerol, an index lipid for biodiesel production was detected. Ten species of $C_{16}{\sim}C_{22}$ with saturated and unsaturated fatty acid were identified showing the very adequate fatty acid profiles for biodiesel production with a good flow property at low temperature. The fractions of glycolipid, neutral and phospholipid were 52.64, 28.10 and 19.26% of total lipid, respectively. The triacylglycerol of 12.63% and chlorophyll a of 49.47% was fractioned using stepwise elution of n-hexane-diethyl ether (95:5, v/v) solvent. The high content of chlorophyll was considered as a potential source of value-added co-product.

Two-Step Oxidation of Refractory Gold Concentrates with Different Microbial Communities

  • Wang, Guo-hua;Xie, Jian-ping;Li, Shou-peng;Guo, Yu-jie;Pan, Ying;Wu, Haiyan;Liu, Xin-xing
    • Journal of Microbiology and Biotechnology
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    • v.26 no.11
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    • pp.1871-1880
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    • 2016
  • Bio-oxidation is an effective technology for treatment of refractory gold concentrates. However, the unsatisfactory oxidation rate and long residence time, which cause a lower cyanide leaching rate and gold recovery, are key factors that restrict the application of traditional bio-oxidation technology. In this study, the oxidation rate of refractory gold concentrates and the adaption of microorganisms were analyzed to evaluate a newly developed two-step pretreatment process, which includes a high temperature chemical oxidation step and a subsequent bio-oxidation step. The oxidation rate and recovery rate of gold were improved significantly after the two-step process. The results showed that the highest oxidation rate of sulfide sulfur could reach to 99.01 % with an extreme thermophile microbial community when the pulp density was 5%. Accordingly, the recovery rate of gold was elevated to 92.51%. Meanwhile, the results revealed that moderate thermophiles performed better than acidophilic mesophiles and extreme thermophiles, whose oxidation rates declined drastically when the pulp density was increased to 10% and 15%. The oxidation rates of sulfide sulfur with moderate thermophiles were 93.94% and 65.73% when the pulp density was increased to 10% and 15%, respectively. All these results indicated that the two-step pretreatment increased the oxidation rate of refractory gold concentrates and is a potential technology to pretreat the refractory sample. Meanwhile, owing to the sensitivity of the microbial community under different pulp density levels, the optimization of microbial community in bio-oxidation is necessary in industry.

Detection of Heparin in the Salivary Gland and Midgut of Aedes togoi

  • Ha, Young-Ran;Oh, So-Ra;Seo, Eun-Seok;Kim, Bo-Heum;Lee, Dong-Kyu;Lee, Sang-Joon
    • Parasites, Hosts and Diseases
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    • v.52 no.2
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    • pp.183-188
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    • 2014
  • Mosquitoes secrete saliva that contains biological substances, including anticoagulants that counteract a host's hemostatic response and prevent blood clotting during blood feeding. This study aimed to detect heparin, an anticoagulant in Aedes togoi using an immunohistochemical detection method, in the salivary canal, salivary gland, and midgut of male and female mosquitoes. Comparisons showed that female mosquitoes contained higher concentrations of heparin than male mosquitoes. On average, the level of heparin was higher in blood-fed female mosquitoes than in non-blood-fed female mosquitoes. Heparin concentrations were higher in the midgut than in the salivary gland. This indicates presence of heparin in tissues of A. togoi.

Monitoring of Degradation Process of Commercial ME Tapes under High Humidity Environment by AC Impedance Techniques

  • Take, Seisho;Shimanuki, Akiko;Itoi, Yasuhiko;Okuyama, Masaru
    • Corrosion Science and Technology
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    • v.3 no.5
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    • pp.194-197
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    • 2004
  • The corrosion resistance of several kind of ME (Metal Evaporated) tape has been investigated both in mild sulfuric acid solution and NaCl solution by electrochemical impedance spectroscopy. It was found that the degradation of ME tapes was accelerated with increasing the concentration of sulfuric acid. There was no significant change in corrosion resistance when the concentration of NaCl was under 3.5 wt%. However, the impedance value decreased when the concentration of NaCl was up to 10 wt%. The degradation of backside of ME tapes was also investigated by AC impedance measurements. The results showed that the impedance behavior of backside plastic film changed with the concentration of sulfuric acid even at the beginning of immersion, implying the changing of the permeability for the backside of ME tapes. It was also found that the corrosion resistance of DVC (Digital Video Cassette) ME tape was better that that of Hi-8mm ME tapes in sulfuric acid solutions. Also, the backside of DVC ME tape showed better water resistance than that of Hi8 ME tapes.

SIFamide and SIFamide Receptor Define a Novel Neuropeptide Signaling to Promote Sleep in Drosophila

  • Park, Sangjin;Sonn, Jun Young;Oh, Yangkyun;Lim, Chunghun;Choe, Joonho
    • Molecules and Cells
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    • v.37 no.4
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    • pp.295-301
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    • 2014
  • SIFamide receptor (SIFR) is a Drosophila G protein-coupled receptor for the neuropeptide SIFamide (SIFa). Although the sequence and spatial expression of SIFa are evolutionarily conserved among insect species, the physiological function of SIFa/SIFR signaling remains elusive. Here, we provide genetic evidence that SIFa and SIFR promote sleep in Drosophila. Either genetic ablation of SIFa-expressing neurons in the pars intercerebralis (PI) or pan-neuronal depletion of SIFa expression shortened baseline sleep and reduced sleep-bout length, suggesting that it caused sleep fragmentation. Consistently, RNA interference-mediated knockdown of SIFR expression caused short sleep phenotypes as observed in SIFa-ablated or depleted flies. Using a panel of neuron-specific Gal4 drivers, we further mapped SIFR effects to subsets of PI neurons. Taken together, these results reveal a novel physiological role of the neuropeptide SIFa/SIFR pathway to regulate sleep through sleep-promoting neural circuits in the PI of adult fly brains.

Draft Genome Sequence of the White-Rot Fungus Schizophyllum Commune IUM1114-SS01

  • Kim, Da-Woon;Nam, Junhyeok;Nguyen, Ha Thi Kim;Lee, Jiwon;Choi, Yongjun;Choi, Jaehyuk
    • Mycobiology
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    • v.49 no.1
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    • pp.86-88
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    • 2021
  • The monokaryotic strain, Schizophyllum commune strain IUM1114-SS01, was generated from a basidiospore of dikaryotic parental strain IUM1114. It even showed the decolorizing activities for several textile dyes much better than its parental strain. Based on the results of a single-molecule real-time sequencing technology, we present the draft genome of S. commune IUM1114-SS01, comprising 41.1 Mb with GC contents of the genome were 57.44%. Among 13,380 protein-coding genes, 534 genes are carbon hydrate-active enzyme coding genes.