• Title/Summary/Keyword: BDOC(Biodegradable Dissolved Organic Carbon)

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Analysis of Attached Bacterial Communities of Biological Activated Carbon Process Using DGGE Method (DGGE 기법을 이용한 생물활성탄 공정의 부착 박테리아 군집분석)

  • Son, Hee-Jong;Choi, Jin-Taek;Son, Hyeng-Sik;Lee, Sang-Joon
    • Journal of Korean Society of Environmental Engineers
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    • v.34 no.8
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    • pp.533-540
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    • 2012
  • The concentration of organic compounds was analyzed at each step of BAC (biological activated carbon) process though BDOC (biodegradable dissolved organic carbon) total/rapid/slow. Further, bacteria communities and biomass concentrations measured DGGE (denaturing gradirnt gel electrophoresis) and ATP (adenosine triphosphate) methods were analyzed. The bed volume of steady state is different based on assessment of organic compounds removal. Bed volumes at steady state in DOC, $BDOC_{rapid}$ and $BDOC_{total/slow}$ removal were around 27,500, 15,000 and 32,000, respectively. A biomass didn't change after the bed volume reached 22,500 according to analyzing HPC (heterotrophic plate count) and ATP concentration of bacteria. The concentration of HPC and ATP were $3.3{\times}10^8$ cells/g and $2.14{\mu}g/g$, respectively. The number of the DGGE band were only 5 at the bed volume 8,916, but increased up to 11 at the bed volume 49,632. As operation time increase, bacterial group were more diversity. Four bacteria species including Pseudomonas fluorescens, the uncultured bacterium similar to Acinetobacteria, uncultured Novosphingobium sp. and Flavobacterium frigidarium have detected from the early stages and Proteobacteria group were dominantly detected.

Analysis of Effects of Factors Influencing Biofilm Formation in Drinking Water Distribution Pipe Using Factorial Experimental Design (요인실험계획을 이용한 수도관 생물막 형성 영향 인자의 효과 분석)

  • Park, Se-Keun;Choi, Sung-Chan;Kim, Yeong-Kwan
    • Journal of Korean Society of Water and Wastewater
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    • v.19 no.2
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    • pp.181-192
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    • 2005
  • This study evaluated the effect of factors influencing the initial biofilm formation in drinking water distribution pipe by running experiments using a $2^{4-1}$ fractional factorial experimental design with a replicate. Important variables used for assessing biofilm formation included BDOC(biodegradable dissolved organic carbon), viable heterotrophic bacteria present in drinking water, water temperature, and shear stress at two levels each. Based on the statistical analysis of biofilm levels measured as attached HPC(heterotrophic plate count) and community-level assay, the main factors that have significant effects on biofilm formation were found to be viable heterotrophic bacteria and BDOC. Water temperature only exhibited significant effect on the levels of attached HPC, while shear stress was not a significant factor under given conditions. Moreover, the statistical analysis revealed that interactions between the important variables were not statistically significant at a 0.05 significance level.

Characterization of Organic Matters Removed by Biological Activated Carbon (생물활성탄처리에서 제거된 유기물 특성)

  • Kim, Woo-Hang;Mitsumasa, Okada
    • Journal of Environmental Science International
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    • v.16 no.6
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    • pp.671-675
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    • 2007
  • The objective of this study was to clarify the characteristics of the removed micropollutant since the breakthrough of adsorption ability was occurred in biological activated carbon(BAC) process. The removal efficiency of DOC (Dissolved Organic Carbon) was 36 % in the breakthrough of BAC occurred by NOM (Natural Organic Matter). The most of removal DOC was found out the adsorbable and biodegradable DOC (A&BDOC). But it was not clear to remove by any mechanism because A&BDOC have simultaneously the adsorption of activated carbon and biodegradation by microorganism in BAC. The removal of bromophenol was examined with BAC and rapid sand filter, for investigation of DOC removal mechanism in the breakthrough of BAC. In this experiment, BAC filter has been operated for 20 months for the treatment of reservoir water. The BAC filter was already exhausted by NOM. Bromophenol, adsorbable and refractory matter, was completely removed by BAC filter. Therefore, it might be removed by the adsorption in BAC. Adsorption isotherms of bromophenol were compared to two BACs which was preloaded with 500 daltons and 3,000 daltons of NOM. BAC preloaded with 3,000 daltons of NOM was not decreased to the adsorbability of bromophenol but BAC preloaded with 500 daltons of NOM was greatly decreased to it. These result indicated that NOM of low molecular weight can be removed by adsorption after a long period of operation and the breakthrough by NOM in BAC. Therefore, micropollutants might be removed through adsorption by saturated BAC.