• Journal of Life Science
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• v.30 no.1
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• pp.88-95
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• 2020

Using a random arbitrarily primed polymerase chain reaction, messenger RNA expression levels were assessed after exposure to 10% (v/v) toluene for 8 hr in solvent-tolerant Pseudomonas sp. BCNU 106. Among the 100 up-expressed products, 50 complementary DNA fragments were confirmed to express repeatedly; these were cloned and then sequenced. Blast analysis revealed that toluene stimulated an adaptive increase in the gene expression level in association with transcriptions such as LysR family of transcriptional regulators and RNA polymerase factor sigma-32. The expression of catalase and Mn2+/Fe2+ transporter genes functionally associated with inorganic ion transport and metabolism increased, and the increased expression of type IV pilus assembly PilZ and multi-sensor signal transduction histidine kinase genes, functionally categorized into signal transduction and mechanisms, was also demonstrated under toluene stress. The gene expression level of beta-hexosaminidase in association with carbohydrate transport and metabolism increased, and those of DNA polymerase III subunit epsilon, DNA-3-methyladenine glycosylase II, DEAD/DEAH box helicase domain-containing protein, and ABC transporter also increased after exposure to toluene in DNA replication, recombination, and repair, and even in defense mechanism. In particular, the RNAs corresponding to the ABC transporter, Mn2+/Fe2+ transporter, and the β-hexosaminidase gene were confirmed to be markedly induced in the presence of 10% toluene. Thus, defense mechanism, cellular ion homeostasis, and biofilm formation were shown as essential for toluene tolerance in Pseudomonas sp. BCNU 106.

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.257-260
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• 2001

BCNU 106, 154 and 171 was isolated in the areas contaminated by high concentrations of organic solvents. These strains had the tolerance to almost all kinds of organic solvents used in this study. Especially, it was interested that BCNU 106, and 171 had the tolerance to meta-, para- and ortho. xylene. And 3 strains had high MIC (minimal inhibition concentrations).

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.590-592
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• 2000

The indole tolerance level of Pseudomonas savastanoi BCNU 106 was as high as 300 mg/ml when toluene or p-xylene was added to the medium to 20% by volume. Pseudomonas savastanoi BCNU 106 grown in a two-phase system containing the various concentrations of indole and solvents produced indigo and indirubin. The optimal condition in the production of indigo and indirubin was also studied.

• Proceedings of the Korean Society of Life Science Conference
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• 2001.09a
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• pp.74-74
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• 2001

• Proceedings of the Korean Society of Life Science Conference
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• 2001.09a
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• pp.77-78
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• 2001

• KSBB Journal
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• v.26 no.2
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• pp.100-106
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• 2011

Strain BCNU 5011 was isolated from forest soil samples collected in the Taebaek mountain in the Gangwon province, Korea. The biochemical, physiological and 16S rRNA sequence analysis strongly indicated that this isolate was most closely related to Paenibacillus polymyxa. A maximum production level of antimicrobial substances of Paenibacillus sp. BCNU 5011 was achieved under aerobic incubation at $30^{\circ}C$ for 3 days in SST broth.Paenibacillus sp. BCNU 5011 showed a broad spectrum of activity against Gram positive and Gram negative bacteria, including methicllinresistant Staphylococcus aureus (MRSA). Paenibacillus sp. BCNU 5011 was also shown to inhibit the growth of different potential human pathogenic bacteria and fungi in vitro. Peptide extract showed better antimicrobial activity than solvent extracts. But active antimicrobial compounds might be included in both peptide extract and solvent extracts. Further separation, purification and identification of active principles leads project to develop antimicrobial agents and anti-MRSA agents.

• Proceedings of the Korean Society of Life Science Conference
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• 2005.09a
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• pp.99-99
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• 2005

• Proceedings of the Korean Society of Life Science Conference
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• 2005.09a
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• pp.100-100
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• 2005

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.368-368
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• 2005

• Proceedings of the Korean Society of Life Science Conference
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• 2003.05a
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• pp.133-133
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• 2003