• Journal of Embryo Transfer
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• v.33 no.4
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• pp.205-210
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• 2018

This study was performed to investigate the effect of types of oil (OVOIL vs. OIL) on B6D2F1 mice oogenesis. In this study, B6D2F1 F1 mice were used in order to maximize oogenesis. The expansion rate of cumulus cells ($82.0%{\pm}0.2$ vs. $78.0%{\pm}0.1$), in vitro fertilization rate ($92.0%{\pm}0.1$ vs. $88.0%{\pm}0.1$), developmental rate ($91.0%{\pm}0.1$ vs. $87.0%{\pm}0.2$), blastocysts formation rate ($56.0%{\pm}0.1$ vs. $57.0%{\pm}0.1$), and zona hatched rate($41.4%{\pm}0.2$ vs. $24.0%{\pm}0.1$) were not different between groups (NS; P>0.05). However, there was a significant difference in maturation rate; the OVOIL group showed higher maturation rate compared to that of the OIL group ($96.0%{\pm}0.1$ vs. $87.0%{\pm}0.1$; P<0.05). In the blastocysts cell numbers, the total cell numbers ($83.9{\pm}26.1$ vs. $56.9{\pm}23.9$), ICM cell numbers ($15.7{\pm}8.8$ vs. $6.3{\pm}3.5$), TE cell numbers ($68.3{\pm}25.7$ vs. $50.7{\pm}24.1$), % ICM ($21.6%{\pm}0.1$ vs. $12.7%{\pm}0.1$), and the ratio of ICM:TE ($1:6.2{\pm}6.5$ vs. $1:10.3{\pm}7.0$) were significantly higher in the OVOIL group than the OIL group (P<0.05). These results suggested that it is expected to achieve the more developmental ability of B6D2F1 mice depending on the type of oil (OVOIL vs. OIL). In addition, the results can provide essential information for culture condition on B6D2F1 mice. Henceforth, thus, it is expected that these results herein might be used for in vitro culture of human embryos.

• Tuberculosis and Respiratory Diseases
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• v.50 no.3
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• pp.334-342
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• 2001

Background : RpoB gene mutations have been found in about 96-98% of rifampicin (RMP)-resistant Mycobacterium tuberculosis. Recent reports confirm that the in laboratory settings a rpoB gene mutation can be used as a surrogate marker for multi-drug resistant tuberculosis. However, its usefulness in clinical applications has not been evaluated. This study was performed to confirm whether mutation analysis of the rpoB gene of M. tuberculosis is useful in clinical settings. Methods : The medical records of 33 patients in whom rpoB gene analysis was conducted using an INNOLiPA Rif. TB assay (LiPA) from June, 1998, to July, 2000, at the Asan Medical Center were retrospectively reviewed in 33 patients. The clinical characteristics in addition to the drug susceptibility and LiPA results were analyzed. The drug susceptibility test was considered as a gold standard method for M. tuberculosis susceptibility and these results were compared with those of the rpoB gene study and sequencing analysis. Sequencing analysis of the rpoB gene was done in cases where there was a discrepancy between the results of the drug susceptibility an d rpoB gene study. Results : The mean age and sex ratio was $42{\pm}18$, and 24:9 (M:F), respectively. There were 19 RMP susceptible (58%) and 14 RMP-resistant cases (42%) according to the rpoB gene study. The mean time from the request to reporting the results of the rpoB gene study was $5.2{\pm}2.6$ days. The mean gap from reporting the rpoB gene study to reporting the susceptibility was $56{\pm}35$ days. Twenty-eight cases (85%) showed identical results compared with the drug susceptibility results, whereas five cases (15%) showed contradictory results. When compared with the sequencing analysis, of the five cases that showed contradictory results, two had LiP A analysis errors and the remaining three were identical to the sequencing results. The rpoB gene study was of assistance in choosing the appropriate drugs in 28 cases (85%). Conclusions : An rpoB gene study using an LiP A assay was useful in rapidly diagnosing RMP-resistant tuberculosis, which enabled a proper choice of the appropriate drugs in clinical practices. However, an LiPA assay always should be performed in conjunction with microscopy, culture, and susceptibility tests.

• Journal of The Korean Society of Agricultural Engineers
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• v.60 no.6
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• pp.73-81
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• 2018

In this study, subsurface discharge performance has been studied through theoretical seepage analysis on four types of culverts that can be installed under the condition of non-excavation, such as (a)perforated pipe(${\Phi}50mm$), (b)perforated pipe+horizontal mat (B50cm) (c)perforated pipe+horizontal mat+vertical gravel(B<10cm), (d)perforated pipe+vertical gravel(B<10cm), and existing typical type (e)perforated pipe with gravel (B40, h=40cm) which can be installed by excavation. The analysis results were as follows. i) Subsurface discharge performance per unit (m) was (a)type 56%, (b) 91%, (c) 96%, (d) 76%, respectively, lower than the value of (e)culvert. ii) However, considering that non-excavation culvert can be installed at a spacing of 5m with the installation cost of the existing excavation culvert at the interval of 10m, it was analyzed that unit subsurface discharge(q) of (a)20.2mm/day(110%), (b)32.8(178%), (c)34.6(188%) (d)27.5(149%) in the four types of non-excavation culvert installed at intervals of 5m under the condition of $ k=10^{-4}cm/s$ was much larger than the amount of (e)type 18.5(100%), existing excavation culvert installed at 10m interval. iii) Through the test construction, peak subsurface drainage discharge($q_p$) was 38.4mm/day, which is larger than the value of design criteria and confirmed that it satisfies the analysis results as well. iv) In particular, it was evaluated that (b)perforated pipe+horizontal mat(B50cm) are low cost, high efficiency subsurface drainage culvert type with sufficient drainage performance(178%).

• Journal of Microbiology and Biotechnology
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• v.33 no.5
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• pp.600-606
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• 2023

Dengue virus (DENV) is a widespread arbovirus. To efficiently establish infection, DENV evolves multiple strategies to hijack the host innate immune response. Herein, we examined the inhibitory effects of DENV serotype 2 (DENV2) nonstructural proteins on RIG-I-directed antiviral immune response. We found that DENV2 NS2A, NS2B, NS4A, and NS4B significantly inhibited RIG-I-mediated IFN-β promoter activation. The roles of NS2B in RIG-I-directed antiviral immune response are unknown. Our study further showed that NS2B could dose-dependently suppress RIG-I/MAVS-induced activation of IFN-β promoter. Consistently, NS2B significantly decreased RIG-I- and MAVS-induced transcription of IFNB1, ISG15, and ISG56. Mechanistically, NS2B was found to interact with MAVS and IKKε to impair RIG-I-directed antiviral response. Our findings demonstrated a previously uncharacterized function of NS2B in RIG-I-mediated antiviral response, making it a promising drug target for anti-DENV treatments.

• Korean Journal of Microbiology
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• v.17 no.4
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• pp.165-178
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• 1979

Phages of Lactobaciilus casei (PLC) isolated from plant drainage were classified and characterized. The results are as follows : 1. On the basis of host range pattern, phages could be divided into 2 groups (PLC-B and PLC-C). PLC-B group phages could be further divided into 5 sub-groups $(B_1, \;B_2, \;B_3, \;B_4, \;and\;B_5)$. Although PLC-C group phages had the same host range, they could be also divided into 2 sub-groups $(C_1\;and\;C_2)$ by morphlogical type. 2. It was $B_3$ group phages that represented a major proportion (44.4%) of phages tested. However, $B_1$ group phages were shown to have the widest host range. 3. Electron micrographs revealed that the phages fell into three different morphological types. $(B_1, \;B_2, \;and\;B_3)$ group phages hd a hexagonal head (52nm in diameter) and a sheathless noncontractile (245 nm in length). $B_4\;and\;C_2$ group phages had a hexagonal head (56 nm) and a short flexible tail (169nm) having no sheath. $B_5\;and\;C_1$ group phages were shown to have a hexagonal head (81 nm) and a contractile tail (140 nm) having a sheath, a base plate and tail fibers. 4. The inactivation of the phages by antisera indicated that serological relationships correlated completely with morphological types. 5. $B_1, \;C_1\;and\;C_2$ group phages produced a large (1, 2 mm in diameter) plaque with a clear ring. The morphology of plaques of $B_3\;and\;B_5$ group phages was the same as those produced by the above, but the average plaque sizes for $B_3\;and\;B_5$ were 0.8 mm abd 0.5 mm, respectively. $B_2\;and\;B_4$ group phages produced a small (0.5 mm) turbid plaque with an irregular edge. 6. The latent period and the average burst size of $B_1\;and\;B_3$ group phages were 90 min and 100, respectively. These phages reuqired calcium ions for their miltiplication. 7. $B_3$ group phages could not be absrobed to R-variant $KC_1$. 8. The order of resistance of phages to heat was $B_2\;>\;B_1, B_4\;and\;B_5\;>\;B_3\;and\;C_2, \;B_5$ group phages were more stable than $B_3$ in various pH values. $C_2$ group phages were more sensitive to UV irradiation than $B_1\;and\;B_3$ group phages. 9. Strains YIT9018 and IAM 1043 were induced by mitomycin C treatment. Phage particles detected in the lysates had a hexagonal head (38 and 49 nm, respectively), but no tail. Any sensitive indicator strain could not be isolated in spite of repaeated trials.

• Journal of Food Hygiene and Safety
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• v.35 no.5
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• pp.477-488
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• 2020

Recently, foodborne illness outbreaks linked to fresh produce are being increasingly reported in the United States, the EU, and Korea as well. Some of this increase may be due to improved surveillance, increase in consumption, change in consumers' habits, and complex distribution systems. Garlic chive is a green, fresh-cut vegetable consumed year-round as a nutrition-rich herb in Korea. It is also prone to contamination with foodborne pathogens during pre-harvest, as amendment with high amounts of livestock manure or compost to soil is required in its cultivation. Our aim in this study was to evaluate microbial contamination of garlic chives, garlic chives cultivation soil, compost, and irrigation water in the southern part of Korea. Samples were collected in A, B, and C regions in 2019 and 2020, and 69, 72, 27, and 40 of garlic chives, soil, compost, and irrigated water, respectively, were analyzed for the presence of sanitary indicator bacteria (total aerobic bacteria, coliforms and Escherichia coli), Bacillus cereus, Staphylococcus aureus, pathogenic E. coli, E. coli O157:H7, Listeria monocytogenes, and Salmonella spp. In A, B, and C regions, levels of total aerobic bacteria, coliform, B. cereus, and S. aureus on all samples were between 1.14 and 8.83 log CFU/g, 0.43 and 5.01 log CFU/g, 0.41 and 5.55 log CFU/g, and 1.81 and 6.27 log CFU/g, respectively. B. cereus isolated from garlic chives and environmental samples showed β-hemolysis activity. Incidence of S. aureus in garlic chive and its production environments in 2020 was different from 2019. In this study, B. cereus and S. aureus were the only pathogenic microorganisms detected in all samples. As a result, this work suggests that continuous monitoring in the production and pre-harvest environment is required to improve hthe hygiene and safety of garlic chive.

• The Journal of the Korean Society for Microbiology
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• v.20 no.1
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• pp.109-113
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• 1985

It was well known that B. pertussis cells possess protective antigen, histamine sensitizing factor, heatstable and labile toxin, hemagglutinin, agglutinogen and the others. Previous reports involving above antigenic properties of B. pertussis have been carried out for several years. However, leucocyte promoting property was not yet investigated. In this report, the results of studies on the leucocytosis, particulary the lymphocytosis, produced in mice by injecting pertussis vaccine were presented. Especially leucocyte promoting property and histamine sensitizing property of B. pertussis vaccine treated at various temperatures were compared. The relationship between the leucocyte promoting property and histamine sensitizing property was investigated. Results were as follows. 1. Although leucocytosis was significantly rised in both 0.5ml injection and 0.1ml injection of pertussis vaccine than in control, at the higher dose (0.5ml injection) an elevation in white cell count was more significant. The leucocyte responce to pertussis vaccine was greater following 0.5 ml injection than following 0.1ml injection. 2. Lymphocytosis was significantly rised in both 0.5ml injection and 0.1ml injection of pertussis vaccine than in control. At higher dose (0.5ml injection), an elevation in lymphocyte count was more significant. 3. Order of elevation in differential leucocyte counts was lymphocyte, polymorphonuclear leucocyte and monocyte. 4. The leucocyte response to pertussis vaccine was 2 fold greater following intravenous injection than following subcutaneous injection. 5. Decrease leucocyte promoting activity and histamine sensitizing activity resulted from exposure to temperature above $56^{\circ}C$. Histamine sensitizing activity of pertussis vaccine treated at various temperatures paralleled leucocyte promoting activity.

• International Journal of Fluid Machinery and Systems
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• v.6 no.2
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• pp.56-74
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• 2013

Stall stagnations in the system of axial-flow compressors and ducts occur in transition from deep surge conditions to decayed or converged stall conditions. The present study is concerned with the boundaries between the deep surges and the stagnation stalls on the basis of analytical results by a code on surge transients analysis and simulation. The fundamental acoustical-geometrical stagnation boundaries were made clear from examinations of the results on a variety of duct configurations coupled with a nine-stage compressor and a single stage fan. The boundary was found to be formed by three parts, i.e., B- and A-boundaries, and an intermediate zone. The B-boundary occurs for the suction-duct having a length of about a quarter of the wave-length of the first resonance in the case of very short and fat plenum-type delivery duct. On the other hand, the A-boundary occurs for the long and narrow duct-type delivery flow-path having a length about a fifth of the wavelength and relatively small sectional area in the case of short and narrow suction ducts. In addition to this, the reduced surge-cycle frequencies with respect to the duct lengths are observed to have respective limiting values at the stagnation boundaries. The reduced frequency for the B-boundary is related with a limiting value of the Greitzer's B parameter. The tendency and the characteristic features of the related flow behaviors in the neighborhood of the boundaries were also made clearer.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.5
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• pp.680-683
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• 2001

The apparent metabolisable energy (AME) and N-corrected ME (AMEn) of deoiled rice bran (DORB) were determined with adult quails at 6 and 10- week of age. The DORB obtained from two types of extraction process, Batch (DORB-B) and the Continuous (DORB-C), was each included in a practical type of the reference diet at 20 or 40% level. The analysed crude protein, ether extract, total ash, calcium, phosphorus, glucose and starch content of DORB-B and DORB-C were found at 19.0, 0.79, 17.05, 0.11, 1.92, 2.3, 11.22, and 15.02, 1.56, 13.0, 0.40, 2.76, 2.16, 19.0, respectively. The level of inclusion of DORB in diet appeared to exert a significant effect on the AME and AMEn values. When bioassayed at 20% inclusion level the DORB was found to have a significantly (p<0.01) lower value than that obtained at 40% inclusion level. However, no significant effect of age of quails on the AME values of DORB was evident. The ME bioassays with quails gave significantly (p<0.01) higher AME values for DORB-C than DORB-B thereby indicating that the continuous system of solvent extraction of rice bran is superior to the batch system from this point of view. The AME value of DORB predicted from its chemical composition also revealed that the DORB-C contained approximately 15% more energy than that in DORB-B.

• The Journal of Fisheries Business Administration
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• v.14 no.1
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• pp.44-56
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• 1983

This study is attempted to serve the fundamental theory for ‘The reorganization of Korean coastal and adjacent water fishery.’ On the Korean coastal and adjacent water fishery where one species stock is catched by multiple fisheries, traditional analysis is not suitable, as analyzing through adjusting the heterogeneous fishing effort among the fisheries to an unit having same fishing strength. Therefore, this study presents the ‘Multi-Variable Model’, adopting fishing effort from each fishery as independent variable, respectively, in order to analyze the quantitative fluctuation of fishery resource not with fishing strength but with amount of fishing effort, measured by the unit of each fishery. For the sake of simplication, this study assumes that one species is catched by two fishery, premise two assumption. 1) Every fishery has not the selectivity in fishing 2) The promotion of fishing efficiency is accomplished in the same speed. Resource equilibrium equation of each fishery is; $$CPUE_1=\frac{Y_1}{E_1}=a_1+ b_1\cdot E_1+c_1\cdot E_1$$ $$CPUE_1=\frac{Y_1}{E_1}=a_1+ b_1\cdot E_1+c_1\cdot E_1$$ Sustainable yield equation is; $$SY_1=a_1\cdot E_1+\cdot b_1E{_1}{^2}+c_1\cdot E_1\cdot E_1$$ $$SY_1=a_1\cdot E_1+b_1\cdot E_1\cdot E_1+c_1\cdot E{_1}{^2}$$ This study is rudimentary, hereafter, refinemental analysis will be supplemented.