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Assessment of In Vitro Oocyte Maturation in Two Gobiid Fish Species, Chasmichthys dolichognathus and Tridentiger trigonocephalus after Exposure to Benzo[a]pyrene

  • Hwang, In-Joon;Baek, Hea-Ja
    • Development and Reproduction
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    • v.15 no.3
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    • pp.223-230
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    • 2011
  • Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental contaminants derived from incomplete combustion of carbons and crude oil. In this study, we investigated the effects of benzo[a]pyrene (B[a]P), a representative PAHs on in vitro sex steroid hormone production and germinal vesicle breakdown (GVBD) using isolated oocytes of longchin goby (Chasmichthys dolichognathus) and chameleon goby (Tridentiger trigonocephalus). Oocytes in diameters of 0.8-0.9 (end vitellogenic stage) and 0.9-1.0 mm (germinal vesicle migratory stage) from longchin goby and 0.5 mm (fully vitellogenic stage) from chameleon goby were used. In GVBD assay, B[a]P at 10 nM stimulated GVBD in the oocytes of 0.8-0.9 mm from longchin goby. B[a]P at 1 nM stimulated GVBD in the oocytes with diameter 0.5 mm from chameleon goby. In steroid production from oocytes of longchin goby, B[a]P at 100 nM decreased testosterone (T) production, B[a]P at 1,000 nM increased estraiol-17 (J (E2) production and 10 and 100 nM increased $17,20{\beta}$-dihydroxy-4-pregnen-3-one ($17{\alpha}20{\beta}P$) production in the oocytes with diameter 0.8-0.9 mm. B[a]P at 1,000 nM increased E2 production, 100 and 1,000 nM increased $17{\alpha}20{\beta}P$ production in the oocytes with diameter 0.9-1.0 mm. In steroid production of oocytes from chameleon goby, B[a]P at 1,000 nM increased $E_2$ production. B[a]P at 10 nM increased $17{\alpha}20{\beta}P$ production. In the ratio of $E_2$ to T ($E_2$/T), B[a]P at 100 and 1,000 nM increased $E_2$/T in the oocytes of longchin goby. B[a]P at 100 nM also increased $E_2$/T in the oocytes of chameleon goby. Taken together, these results suggest that B[a]P have not only weak estrogenic effects but progestogenic effects on oocyte maturation.

Preparation of B4C-Al2O3 Composite Powder by Self-propagation High-temperature Synthesis(SHS) Process under High Pressure (고압 자전 고온반응 합성법에 의한 B4C-Al2O3복합분말 제조)

  • 임경란;강덕일;김창삼
    • Journal of the Korean Ceramic Society
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    • v.40 no.1
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    • pp.18-23
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    • 2003
  • Composite powder of$B_4C-A1_2O_3$was prepared from a mixed powder of$B_2O_3/A1/C$by SHS under argon pressure instead of using a chemical furnace. A mixture of$B_2O_3,$Al and C powder (equivalent amounts to the reaction,$2B_2O_3+4A1+C=B_4C+2A1_2O_3)$was ball milled for 2 h. The mixed powder was placed in a SHS reactor and filled with 10 atm of argon gas and ignited. The inner and outer products were the same by XRD analysis. It was consisted of a composite powder of$B_4C-A1_2O_3$without $AlB_{12}/C_2$which was always produced using a chemical furnace. The composite powder was about$60~100{mu}m$size which was composed of crystalline particles of about 0.3~l${mu}m$size. But when 15 atm of argon was employed, partial sintering took place to give rise hard composite powder of$15~25{mu}m$$B_4C$with $0.1~0.2{mu}m$$A1_2O_3.$

A 0.13 ㎛ CMOS Dual Mode RF Front-end for Active and Passive Antenna (능·수동 듀얼(Dual) 모드 GPS 안테나를 위한 0.13㎛ CMOS 고주파 프론트-엔드(RF Front-end))

  • Jung, Cheun-Sik;Lee, Seung-Min;Kim, Young-Jin
    • Journal of Advanced Navigation Technology
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    • v.13 no.1
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    • pp.48-53
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    • 2009
  • The CMOS RF front-end for Global Positioning System(GPS)are implemented in 1P8M CMOS $0.13{\mu}m$ process. The LNAs consist of LNA1 with high gain and low NF, and LNA2 with low gain and high IIP3 for supporting operation with active and passive antenna. the measured performances of both LNAs are 16.4/13.8 dB gain, 1.4/1.68 dB NF, and -8/-4.4 dBm IIP3 with 3.2/2 mA form 1.2 V supply, respectively. The quadrature downconversion mixer is followed by transimpedance amplifier with gain controllability from 27.5 to 41 dB. The front-end performances in LNA1 mode are 39.8 dB conversion gain, 2.2 dB NF, and -33.4 dBm IIP3 with 6.6 mW power consumption.

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Effect of UV-B Irradiation on Vitamin $D_2$ Contents, Color Value and Flavor Pattern in Pleurotus ostreatus (자외선 B파 조사가 느타리버섯의 비타민 $D_2$ 함량, 색도 및 향 패턴에 미치는 영향)

  • Lee, Jin-Sil
    • Korean journal of food and cookery science
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    • v.23 no.1 s.97
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    • pp.99-106
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    • 2007
  • This study investigated the effect of UV-B irradiation on the quality of Pleurotus ostreatus. The changes of vitamin $D_2$ contents, color value and flavor pattern in mushrooms were analyzed by high-performance liquid chromatography (HPLC), chromameter and gas chromatography - surface acoustic wave (GC-SAW) electronic nose. By exposure to UV-B irradiation (0 kj/m$^2$, 10 kj/m$^2$, 20 kj/m$^2$), vitamin $D_2$ content increased from 0 (control) to 48.50 g/g (DM: dry matter, 10 kj/m$^2$) and 61.58 g/g (DM, 20 kj/m$^2$). Although there was no significant difference in L, a, b values among the three groups, flavor changes were detected by GC-SAW electronic nose. The number of peaks increased from 10 in the control group (0 kj/m$^2$), to 14 and 15 for the 10 kj/m$^2$ and 20 kj/m$^2$ groups, respectively. Nevertheless, the changes of flavor pattern were not detrimental to the mushroom quality. These results suggested that UV-B irradiation is an effective method to increase the vitamin $D_2$ content without degrading the quality.

SOME NEW IDENTITIES CONCERNING THE HORADAM SEQUENCE AND ITS COMPANION SEQUENCE

  • Keskin, Refik;Siar, Zafer
    • Communications of the Korean Mathematical Society
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    • v.34 no.1
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    • pp.1-16
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    • 2019
  • Let a, b, P, and Q be real numbers with $PQ{\neq}0$ and $(a,b){\neq}(0,0)$. The Horadam sequence $\{W_n\}$ is defined by $W_0=a$, $W_1=b$ and $W_n=PW_{n-1}+QW_{n-2}$ for $n{\geq}2$. Let the sequence $\{X_n\}$ be defined by $X_n=W_{n+1}+QW_{n-1}$. In this study, we obtain some new identities between the Horadam sequence $\{W_n\}$ and the sequence $\{X_n\}$. By the help of these identities, we show that Diophantine equations such as $$x^2-Pxy-y^2={\pm}(b^2-Pab-a^2)(P^2+4),\\x^2-Pxy+y^2=-(b^2-Pab+a^2)(P^2-4),\\x^2-(P^2+4)y^2={\pm}4(b^2-Pab-a^2),$$ and $$x^2-(P^2-4)y^2=4(b^2-Pab+a^2)$$ have infinitely many integer solutions x and y, where a, b, and P are integers. Lastly, we make an application of the sequences $\{W_n\}$ and $\{X_n\}$ to trigonometric functions and get some new angle addition formulas such as $${\sin}\;r{\theta}\;{\sin}(m+n+r){\theta}={\sin}(m+r){\theta}\;{\sin}(n+r){\theta}-{\sin}\;m{\theta}\;{\sin}\;n{\theta},\\{\cos}\;r{\theta}\;{\cos}(m+n+r){\theta}={\cos}(m+r){\theta}\;{\cos}(n+r){\theta}-{\sin}\;m{\theta}\;{\sin}\;n{\theta},$$ and $${\cos}\;r{\theta}\;{\sin}(m+n){\theta}={\cos}(n+r){\theta}\;{\sin}\;m{\theta}+{\cos}(m-r){\theta}\;{\sin}\;n{\theta}$$.

Assessing the Viability of Rat Oocyte by Use of Eosin B (Eosin-B에 의한 rat 난모세포의 생존성 검사)

  • 김명철;윤효인;김무강
    • Korean Journal of Animal Reproduction
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    • v.14 no.1
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    • pp.31-35
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    • 1990
  • The viability and developmental potential of rat oocyte was assessed by the use of eosin B. Dye was prepared as a stock solution to contain 1.0 mg eosin B per ml of Brinster's Medium for Ovum Culture plus glucose (BMOC) -2 medium. Three dye concentrations were selected for the Oocytes from rat ovarian follicles were collected and assessed under various conditions of heat oocyte studies by dilution of the stock solution with BMOC: 0.12mM, 0.60mM and 1.20mM. treatment and preservation duration by dye exc¬lusion assay using eosin B. It is suggested that live oocyte excludes eosin B whereas dead oocyte does not.

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The Enhancement of Cytochrome P-450 Mediated Aryl Hydroxylation of Bentazon in Rice Microsomes (벼에서 Bentazon 히드록시화반응에 관련된 Cytochrome P-450 활성(活性) 증진(增進)에 관한 연구(硏究))

  • Pyon, J.Y.;Balke, N.E.
    • Korean Journal of Weed Science
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    • v.17 no.1
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    • pp.59-65
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    • 1997
  • Bentazon 6-hydroxylase (B6H) and cinnamic acid 4-hydroxylase (CA4H) activities were determined in rice (Oryza sativa L.) microsomes to study methods of enhancing cytochrome P-450 mediated aryl hydroxylation of bentazon by hydoxylase inducing compounds. Pretreating rice seeds with 1,8-naphthalic anhydride at 0.5-2% and fenclorim at 5 and 10 ${\mu}M$ increased B6H and CA4H activities. Treatments of rice seedling with ethanol 2.5% enhanced B6H and CA4H activities, and with phenobarbital at 12 mM enhanced B6H activity, and CA4H activity was enhanced at 2 mM. B6H activity was synergistically enhanced by combined treatments of ethanol 2.5 or 5% and phenobarbital 8 or 12mM and also that of 1,8-naphthalic anhydride 0.5 or 1% and phenobarbital 8 or 12 mM, but CA4H activity was decreased by combined treatment. Five-day-old rice seedlings showed higher B6H and CA4H activities which decreased with seedling age.

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Modular Exponentiation by m-Numeral System (m-진법 모듈러 지수연산)

  • Lee, Sang-Un
    • The KIPS Transactions:PartC
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    • v.18C no.1
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    • pp.1-6
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    • 2011
  • The performance and practicality of cryptosystem for encryption, decryption, and primality test is primarily determined by the implementation efficiency of the modular exponentiation of $a^b$(mod n). To compute $a^b$(mod n), the standard binary squaring still seems to be the best choice. But, the d-ary, (d=2,3,4,5,6) method is more efficient in large b bits. This paper suggests m-numeral system modular exponentiation. This method can be apply to$b{\equiv}0$(mod m), $2{\leq}m{\leq}16$. And, also suggests the another method that is exit the algorithm in the case of the result is 1 or a.

CTIO 4m SDSS $u$와 CTIO 1m B filter의 투과함수 특성 및 CTIO 1m Y4KCam의 crosstalk

  • Heo, Hyeon-O;Im, Beom-Du;Seong, Hwan-Gyeong;Bessel, Michael S.
    • The Bulletin of The Korean Astronomical Society
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    • v.37 no.2
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    • pp.155.2-155.2
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    • 2012
  • CTIO 4m 및 CTIO 1m 망원경으로 Westerlund 2의 UBVI 관측을 수행하여, CTIO 4m SDSS $u$ filter의 적색광누출 현상을 발견하였고 그 영향을 분석하였다. 적색광누출 현상은 filter의 투과함수가 설계와는 달리 장파장 영역에서 투과 존재하는 현상으로, CTIO 4m SDSS $u$ filter의 경우 B-V>1.4, V-I>2.0에서 그 영향이 나타나기 시작한다. SDSS $u$ filter의 적색광누출 현상은 별의 고유색지수와 상관없이 관측된 색지수가 클수록 영향이 크며, $B-V{\leq}1.8$, $V-I{\leq}2.8$의 범위에서는 보정이 가능하다. CTIO 1m B filter에서는 성간소광을 받지 않은 별과 성간소광을 많이 받은 별의 표준계변환 결과, $B_{CTIO1m}=B_{Standard}-0.055{\times}E(V-V)$에 해당하는 차이를 보였다. 이러한 차이는 CTIO 1m B filter의 투과함수의 단파장 쪽 날개부분이 표준 Johnson B filter에 비하여 단파장 쪽으로 많이 치우쳐있기 때문으로 보인다. 특히 Ballmer jump에 해당하는 파장인 370 nm에서 filter의 최대투과율에 비하여 32.2%에 달하는 투과율을 보이는데, 이는 Bessell B filter의 3.1%에 비하여 매우 큰 값이다. CTIO 1m 망원경의 Y4KCam CCD에서는 포화된 화소에 의한 crosstalk 뿐 아니라 포화되지 않은 화소에 의한 crosstalk 현상도 보였다. 짧은 노출을 준 영상에서는 5000 ADU 이상에서는 육안에 의한 crosstalk 확인이 가능하며, 포화되지 않은 밝은 별에 의한 crosstalk을 확인하지 않고 측광할 경우 백색왜성으로 오인할 가능성이 있으므로 측광 과정에서 좌표를 통하여 확인할 필요가 있다.

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C32-CONSTRUCTION ON Mn(κ)

  • Song, Youngkwon
    • Korean Journal of Mathematics
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    • v.12 no.1
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    • pp.23-32
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    • 2004
  • Let (B, $m_B$, ${\kappa}$) be a maximal commutative ${\kappa}$-subalgebra of a matrix algebra $M_n(\kappa)$. We will construct a maximal commutative ${\kappa}$-subalgebra (R, $m$, ${\kappa}$) of $M_n+3(\kappa)$ from the algebra B such that the algebra R has dimension greater than the dimension of B by 3. Moreover, we will show a $C_i$-construction doesn't imply a $C^3_2$-construction for $i=1,2$.

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