• Archives of Pharmacal Research
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• v.16 no.4
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• pp.259-264
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• 1993

Three cell lines, CHO, L929 and B16 which are non-tumorigenic and cancer cells, respectively, were first tested for their survival in the presence of radioprotective ginseng protein fraction(GPF0. The influence of three radioprotectors-CPF, cysteamine, and 1-Methyl-2-bis[(2-methylthio)vinyl] quinolinium iodide (MVQI) on DNA repair capacity of UV damaged cells survival test, the GPF showed higher cytotoxicity in L929 and B16 than in CHO cells. However, the degree of cell killing was also investigated by measuring $^3H$-thymidine incorporation of PUVA treated cells. In cell survival test, the GPF showed higher cytotoxicity in L929 and B16 than in CHO cells. However, the degree of cell killing was not high enough to consider it as an antitumorigenic agent. Variable results were obtained in the effects on DNA repair capacity depending on the protectors and cell lines used. In pretreatment, the presence of GPF and MVOI brought about a sinificant increase in the capacity in both CHO and B16 cells. However, in L929, the enhancing effect was not shown. In all three cell lines, cysteamine showed lower repair capacity than control, suggesting the primary damage reduction in stronger enhancing effects in L929 and B16 cells, while it was weaker in CHO cells. Here also cystemine hsowed a very little or no increase in the capacity in all three cell lines. These results demonstrate that GPF has mild cytotoxicity in tumorignic cells and that GPF and MVQI enhance DNA repair capacity of UV damaged cells, whether they are tumorigenic or not. On the other hand, cysteamine shows only damage reduction effect. Celles of different genetic origin seem to give different responses to the modifier and different modifiers may possibly work by different mechanisms.

• Fibers and Polymers
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• v.3 no.1
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• pp.8-13
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• 2002

The effect of block sequence on the self-assembly of ABC-type triblock copolymers in the ordered state is investigated using an isothermal-isobaric molecular dynamics simulation. The block sequence has an important effect ,on the ]norphology of ABC triblock copolymers. Different morphologies are observed depending on the block sequence as well as the block composition. The triblock copolymers with the volume fraction of 1 : 1 : 1 ($f_A$=$f_B$=$f_C$= 0.33) show the three phase and four layered lamellar structures irrespective of the block sequence. The $A_{32}$$B_{16}$$C_{32}$triblock copolymer with $f_B$=0.2 shows a morphology In which cylinders of midblock B are formed at the interface between A and C lamellae, whereas the morphology of triblock copolymer $B_{16}$$C_{32}$ $A_{32}$ and $C_{32}$ $A_{32}$ $B_{16}$ show a cylindrical core-shell structure and a lamellar type morphology, respectively. The $A_{20}$$B_{40}$$C_{20}$the triblock copolymer with the block B as a major component shows a tricontinuous structure, whereas both $B_{40}$$C_{20}$$A_{20}$ and $C_{20}$$A_{20}$$B_{40}$ triblock coolymers exhibit the lamellar structures. When the block B has larger volrome fraction with $f_B$=0.75, the matrix is composed of block B, and other two blocks A and C form spherical domains.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.23 no.4
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• pp.189-197
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• 1987

As the basic research for the application of a parametric acoustic source to fish finding, the characteristics of beam patterns and parametric gains of the acoustic source were investigated and target strengths of fish, grey mullet, with the acoustic source were measured. The mean primary frequency of the acoustic source was 200KHz and the produced sounds by difference-frequencies were 5KHz, 10KHz, 16KHz and 20KHz. For measurement of target strength in yaw (coronal) plane of fish the to be target was 34cm in length, the pulse duration of the source was 0.3m/sec and the difference frequency was 10KHz in consideration of the length of fish and of parametric gain of the acoustic source. The results obtained are as follow: 1. Beam widths(down 3 dB) of the parametric acoustic source excited at frequencies of 5KHz, 10KHz, 16KHz, and 20KHz were 4.3$^{\circ}$, 2.2$^{\circ}$, 3.0$^{\circ}$ and 2.5$^{\circ}$ respectively. 2. Parametric gains of the parametric acoustic source excited at frequencies of 5KHz, 10KHz, 16KHz and 20KHz were -41 dB, -45 dB, -60 dB and -68 dB respectively. 3. Target strengths of a fish in head and tail aspect using the parametric acoustic source were 5 dB lower than those using 200KHz single frequency sound, but those in side aspect were similar. 4. Target strengths of two or three fish with the parametric acoustic source were 1-3 dB lower than those in head and tail aspect using 200KHz single frequency sound.

• Journal of the Korean Society of Food Science and Nutrition
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• v.21 no.2
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• pp.163-170
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• 1992

Color of kimchi juice was measured instrumentally to evaluate the quality of Korean cabbage kimchi during fermentation at 4$^{\circ}C$ and 16$^{\circ}C$ . These results were compared with those of chemical analyses of kimchi juice and sensory evaluation for kimchi. Chemiral analyses and sensory evaluation showed that the kimchi has reached pH 4.3, the pH value under an optimum ripening period, after 3-days fermentation at 16$^{\circ}C$ with almost the highest contents of vitamin C and carotenoids as well as desirable sour taste and texture. At 4$^{\circ}C$ the kimchi was unripened after 6-days fermentation. At 16$^{\circ}C$, $L^{*}$ value of kimchi juice did not show any significant change during fermentation periods. However, $a^{*}$ value increased until day 4$^{\circ}C$ and thereafter decreased sharply. $b^{*}$ value increased until day 3 and then showed no change. High correlations were seen between volume of kimchi juice, pH, total acidity and $L^{*}$or $b^{*}$ value, and between carotenoid content and $a^{*}$ value, respectively. It was desirable to use color $a^{*}$ or $b^{*}$ value of kimchi juice to evaluate the quality of kimchi. The kimchi was under an over-ripening period when $a^{*}$ value was equal to or lower than the initial value, or when $b^{*}$ value was almost constant. Predicting an optimum ripening period was possible by using a proportional value of $a^{*/}$ $b^{*}$ , i.e., unripening period, 1 $\geq$ and near 1 ; optimum ripening period, near 0.8 ; over-ripening period, < 0.8.eriod, < 0.8.d, < 0.8.

• Radiation Oncology Journal
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• v.17 no.2
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• pp.158-165
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• 1999

Purpose : To investigate the percentage of colonies wi1h16or more cells distribution of human skin fibroblast according to in vitro aging, and to evaluate the relationship between percentage of colonies with 10 or more cells and in vivo donor age in human skin fibroblast culture. Material and Method : C1, C2, C3a, and C3b human skin fibroblast samples from three breast cancer patients were used as subjects. The C1, C2, and C3a donor were 44, 54, and 55 years old, respectively. C3a and C3b cells were isolated from the same person. Single cell suspension of skin fibroblasts was prepared with primary explant technique. One hundred cells are plated into 100m1 tissue culture flask and cultured for two weeks. The colony size was defined as colonies with 16 or more cells. The cultured cell was stained with crystal violet, and number of cells in each colony was determined with stereo microscope at $\times$10 magnification. Passage number of C1, C2, C3a and C3b skin fibroblast were 12th, 17th, and 14th, respectively. Results : Percentage of colonies with 16 or more cells of skin fibroblast samples decreased with increasing in vitro passage number. In contrast, cumulative population doublings of skin fibroblast sample increased with increasing in vitro passage number. Percentage of colonies with 16 or more cells also decreased with increasing population doublings in human skin fibroblast culture. There was strong correlation with percentage of colonies with 16 or more cells and population doublings En C3a skin fibroblast sampie. At the same point of population doublings, the percentage of colonies with 16 or more cells of the young C1 donor was higher level than the old C3a donor. Conclusion : The population doublings increased with increasing in vitro passage number but percentage of colonies with 16 or more cells decreased. The results of this study imply that percentage of colonies with 16 or more cell is useful as a indicator of in vitro human skin fibroblast aging and may estimate the in vivo donor age.

• The Journal of the Acoustical Society of Korea
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• v.9 no.6
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• pp.5-13
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• 1990

본 논문에서는 16Kbps 및 32 Kbps 전송속도에서 ADM의 음질을 개선하기 위하여 두 가지 방 식을 적용한다. 첫째로, 고차 예측기 또는 적응 예측기를 ADM에 활용한다. ADM의 경우에 2차 또는 3 차 예측기를 사용하면 16Kbps 전송속도에서는 별로 개선이 없지만 32Kbps 전송속도에서는 SQNR\sub SEG\척도로 약 3-4dB의 상당한 이득이 얻어진다. 또한 ADM에 적응 예측기를 활용하면 최대 성능은 SZNR\sub SEG\ dir 2dB 정도 개선되지만 양자화 잡음의 축적 때문에 동작 범위가 매우 좁아진다. 둘 째로, 지연 결정 방식을 ADM에 이용한다. 지연 결정 방식을 2차 예측기를 갖고 있는 ADM에 적용하면 약 2dB 정도 개선되지만 양자화 잡음의 축적 때문에 동작 범위가 매우 좁아진다. 둘째로 지연 결정 방 식을 ADM 에 이용한다. 지연 결정 방식을 2차 예측기를 갖고 있는 ADM에 적용하면 1차 예측기를 갖 고 있는 ADMDP 적용했을 때 보다 16또는 32Kbps일 때 SQNR\sub SEG\척도로 재래의 ADM 보다 약 5dB 정도의 성능 개선이 얻어진다.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.32 no.5A
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• pp.490-496
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• 2007

This paper describes the design and implementation of an integrated LTCC front-end module for the IEEE802.11a WLAN applications by performing the behavioral-level simulation using measurement-based behavioral model. To meet the IEEE802.11a WLAN standard, a system transmitting 1024 symbols through 64-QAM process at the rate of 54Mbps should be implemented and nonlinear properties are confirmed by simulations of ACPR and EVM in this circumstance. The right offsets of ACPR which are 30MHz, 20MHz, and 11MHz distant from the center frequency of 5.8GHz are 49.36dBc, 36.90dBc, and 24.58dBc, respectively. The left offsets are 50.14dBc, 30.04dBc, and 28.85dBc, respectively and EVM is 2.94%. The size of the module implemented with LTCC five-layer substrates is $13.4mm{\times}14.2mm$. The measured characteristics of the transmitter show P1dB of 16.2dBm and power gain of 16.73dB. Those of the receiver exhibit the small signal gain of 16.24dB and noise figure of 7.83dB.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.12
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• pp.1887-1894
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• 2007

In this paper, a rapid and reliable gene-targeted species-specific polymerase chain reaction (PCR) technique based on a two-step process was established to identify bifidobacteria in dairy products. The first step was the PCR assay for genus Bifidobacterium with genus specific primers followed by the second step, which identified the species level with species-specific primer mixtures. Ten specific primer pairs, designed from nucleotide sequences of the 16-23S rRNA region, were developed for the Bifidobacterium species including B. angulatum, B. animalis, B. bifidum, B. breve, B. catenulatum, B. infantis, B. longum, B. minimum, B. subtile, and B. thermophilum. This technique was applied to the identification of Bifidobacterium species isolated from 6 probiotic products, and four different Bifidobacterium spp. (B. bifidum, B. longum, B. infantis, and B. breve) were identified. The findings indicated that the 16S-23S rDNA gene-targeted species-specific PCR technique is a simple and reliable method for identification of bifidobacteria in probiotic products. PCR combined with Denaturing Gradient Gel Electrophoresis (DGGE) for identification of the bifidobacteria was also evaluated and compared with the gene-targeted species-specific technique. Results indicated that for fermented milk products consistency was found for both species-specific PCR and PCR-DGGE in detecting species. However, in some lyophilized products, the bands corresponding to these species were not visualized in the DGGE profile but the specific PCR gave a positive result.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.2
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• pp.785-790
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• 2013

Background: Persistent infection of one or more of about 15 high-risk human papillomaviruses (HR-HPVs), most commonly HPV types 16/18, has a significant role in cervical cancer initiation and progression. There are limited data available from north-east India about HPV prevalence though this region has high incidence rates of cervical cancer. The aim of this study was to investigate the HPV genotypes prevalent in cervical cancer patients of north-east India. Materials and Methods: We analyzed 107 cervical cancer patient samples. Nested multiplex PCR assays were employed for detection of 13 high risk and 5 low risk HPV types. Results: HPV was confirmed in 105 samples. The presence of 6 'carcinogenic' HPV types, HPV-16 (88%), -18 (15%), -31(4%),-45 (3%), -59 (4%), -58(1%), and one non carcinogenic, HPV-6/11 (6%), was recorded. Among various demographic and clinical factors only tumour stage showed a statistically significant association with HPV type infection (P=0.019). Conclusions: We suggest that the most prevalent genotype is HPV-16 followed by HPV-18 in cervical carcinoma patients of the north-eastern region of India. Advanced tumour stage may be associated with increased possibility of harbouring multiple HPV genotypes.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.15
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• pp.6535-6539
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• 2015

This study was designed to assess, whether a new chemotherapeutic microtubule inhibitor, Epothilone B (EpoB, Patupilone), can induce DNA damage in normal ovarian cells (MM14.Ov), and to evaluate if such damage could be repaired. The changes were compared with the effect of paclitaxel (PTX) commonly employed in the clinic. The alkaline comet assay technique and TUNEL assay were used. The kinetics of DNA damage formation and the level of apoptotic cells were determined after treatment with IC50 concentrations of EpoB and PTX. It was observed that PTX generated significantly higher apoptotic and genotoxic changes than EpoB. The peak was observed after 48 h of treatment when the DNA damage had a maximal level. The DNA damage induced by both tested drugs was almost completely repaired. As EpoB in normal cells causes less damage to DNA it might be a promising anticancer drug with potential for the treatment of ovarian tumors.