• Title/Summary/Keyword: B. mori

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Molecular Cloning and Characterization of the Gene encoding $\beta$-N-acetylhlucosaminidases Homologue from Bombyx mandarina (멧누에(Bombyx mandarina)로부터 $\beta$-N-Acetyglunosamicidase를 코딩하는 cDNA의 분리 및 염기서열 결정)

  • 구태원;황재삼;성규병;윤은영;방혜선;권오유
    • Journal of Sericultural and Entomological Science
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    • v.41 no.3
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    • pp.147-153
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    • 1999
  • Chitinolytic enzymes such as ${\beta}$-N-acetylglucosaminidase are major hydrolases involved in insect molting. We have isolated, sequenced a CDNA encoding ${\beta}$-N-acetylglucosaminidase from the silkworm, Bombyx mandarina, and compared its sequence with genes encoding chitinolytic enyzmes from other sources. The insert DNA in the clone is 3,284 nucleotides long with an open reading frame of 1,788 nucleotides that encodes a protein of 596 amino acids with a molecular weight of 68.2 kDa. There is a 3’-untranslated region composed with 1.479 nucleotides and are several potential polyadenylation signals. The predicted amino acid sequence apparently contains a leader peptide of 23 amino acids. A search of the amino acids sequence databases for sequences similarities to other ${\beta}$-N-acetylglucosaminidases or ${\beta}$-N-acetylhexosaminidases. The highest similarity matched with the enzyme from B. mori, which has a sequence identity of 95%. On the other hand, the identity between the B. mandarina enzyme and those from M. sexta and human are 70% and 24%, respectively.

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Consumption and Conversion Efficiency of Food in New Elite Bivoltine Hybrid Silkworm, Bombyx mori L. under Restricted Feeding Levels

  • Mathur, Vinod B.;Rahmathulla, V.K.;Bhaskar, O.Vijaya
    • International Journal of Industrial Entomology and Biomaterials
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    • v.5 no.2
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    • pp.213-216
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    • 2002
  • Food consumption and conversion efficiency of new bivoltine hybrids (CSR2$\times$CSR4 and CSR2$\times$CSR5) were studied under restricted feeding levels (10, 20 and 30% less quantity of mulberry leaves). The data were compared with a control fed with standard quantum of feed as per the recommendation. The nutritional indices parameters i. e. ingests, digesta, approximate digestibility (%) and reference ratio were recorded higher in control batches compared to less feed batches while nutritional efficiency parameters i. e., ECI and ECD to cocoon and shell were recorded significantly higher in restricted feeding level batches. This increase is attributed due to the physiological adaptation under nutritional stress condition.

Analysis of a PM Motor Drive System by a Coupled Method with MATLAB and FEM

  • Ishikawa T.;Sunaga T.;Nakamura S.;Mori D.;Hashimoto S.;Matsunami M.
    • KIEE International Transaction on Electrical Machinery and Energy Conversion Systems
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    • v.5B no.3
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    • pp.229-232
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    • 2005
  • Finite element method (FEM) is a very powerful tool for the calculation of magnetic field of electromagnetic devices. MATLAB/Simulink is also well known as a very useful tool for control systems. This paper proposes a very promising method, where the FEM is coupled with MATLAB. We apply this method to analyze a permanent magnet (PM) motor drive system, and compare with results using MATLAB only.

GENERATION OF MAGNETIC FIELDS IN COSMOLOGICAL SHOCKS

  • MEDVEDEV MIKHAIL V.;SILVA LUIS O.;FIORE MASSIMILIANO;FONSECA RICARDO A.;MORI WARREN B.
    • Journal of The Korean Astronomical Society
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    • v.37 no.5
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    • pp.533-541
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    • 2004
  • The origin of magnetic fields in the universe remains an outstanding problem in cosmology. We propose that these fields are produced by shocks during the large-scale structure formation. We discuss the mechanism of the field generation via the counter-streaming (Weibel) instability. We also show that these Weibel-generated fields are long-lived and weakly coupled to dissipation. Subsequent field amplification by the intra-cluster turbulence may also take place, thus maintaining the magnetic energy density close to equipartition.

Molecular Cloning and Characterization of the Gene Encoding Chitinase from Bombyx mandarina (멧누에(Bombyx mandarina)로부터 Chitinase를 코딩하는 cDNA의 분리 및 염기서열 결정)

  • 구태원;황재삼;성규병;윤은영;방혜선;권오유
    • Journal of Life Science
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    • v.9 no.4
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    • pp.341-347
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    • 1999
  • Insects use chitinolytic enzyme to digest chitin in the exoskelton during the molting process. We have isolated and sequenced a chitinase-encoding cDNA from the silkworm, Bombyx mandarina, compared its sequenced with genes encoding chitinolytic enzymes from other sources. The insert DNA in the clone is 2,675 nucleotides long with an open reading frame of 1,695 uncletides that encodes a protein of 565 amino acids with a molecuar weight of 63.4 kDa. The 3' -untranslated region of 889 nucleotides is AT-rich and contains two putative polyadenylation signals. The N-terminal sequence of the encoded protein contains numerous hydrophobic residues characteristic of a leader peptide. The amino acid alignment revealed that the endo-$\beta$-N-acetylglucosaminidase had 83% and 97% homology with M. sexta and B. mori, respectively. The deduced amino acid had two highly conserved region at the amino acid residues 97-111 and 139-148 that were related to the existing chitinase.

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Physiological study of Bombyx mori L. due to spraying Agricultural Chemicals on mulberry leaf (농약살포상엽이 누에의 생리에 미치는 영향)

  • 김주읍
    • Journal of Sericultural and Entomological Science
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    • v.2
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    • pp.83-88
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    • 1962
  • These works were carried on to study the damage due to agricultural chemicals on silkworm and to find out the best ways to use them, considering, in the horticultural field, the utilization of them has rapidly been increased. The results may be summarized as follows: 1) The mulberry leaves being more than 100m from the horticultural field used the agricultural chemicals did not harm the silkworm. 2) Even if the mulberry leaves were located less than loom from the horticultural field sprayed with the chemicals, the silkworms were not injured after washing the mulberry leaves which were attached with the chemicals. 3) The chemicals, E.P.N., B. H. C. 2%, and Selesan were more seriously injured to silkworm than the other ones.

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Immunological Relationship of Crystal Proteins of Six Bacillus thuringiensis Serovarieties; B. thuringiensis serovar. coreanensis (H25), konkukian (H34), leesis (H33), seoulensis (H35), sooncheon (H41) andyosoo (H18a18c)

  • Lee, Kwang-Yong;Kang, Eun-Young;Kwon, Hyuk-Han;Lee, Hyung-Hoan
    • Journal of Microbiology and Biotechnology
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    • v.14 no.2
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    • pp.415-416
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    • 2004
  • Crystals of six new Bacillus thuringiensis serovarieties [coreanensis (H25), konkukian (H34), leesis (H33), seoulensis (H35), sooncheon (H41), and yosoo (HI8a18c)] with different H-antigens, which are toxic to Bombyx mori and/or mosquito larvae, were serologically quite distinct from each other.

Aeroelastic behavior of nano-composite beam-plates with double delaminations

  • Mousavi, S.B.;Yazdi, Ali A.
    • Steel and Composite Structures
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    • v.33 no.5
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    • pp.653-661
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    • 2019
  • In this paper aeroelastic behavior of 3-phase nano-composite beam-plate with double delaminations is investigated. It is tried to study the effect of carbon nano-tubes (CNTs) on critical flutter pressure of reinforced damaged nano-composite structures. In this case, the CNTs are appending to the polymer matrix uniformly. The Eshelby-Mori-Tanaka model is used to obtain the effective material properties of 3-phase nano-composite beam-plate. To investigate the aeroelastic behavior of delaminated beam-plate subjected to supersonic flow, it is assumed that the damaged segments are forced to vibrate together. The boundary conditions and auxiliary conditions at edges of delaminated segments are used to predict critical flutter pressure. The influence of CNTs and different delamination parameters such as delamination length, axial position and its position through thickness are investigated on critical flutter pressure.

Hemolymph Juvenile Hormone Binding Protein of Fifth Instar Larvae of Bombyx mori L.: Identification and Purification (누에나방의 5령유충 혈림프의 유약호르몬 결합단백질: 확인 및 정제)

  • Park, Chul-Ho;Kim, Hak-Ryul
    • The Korean Journal of Zoology
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    • v.37 no.1
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    • pp.66-75
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    • 1994
  • Juvenile hormone binding protein was identified in the hemolymph of fifth instar larvae and purified using column chromatography. Hemolymph was mixed with [3H] JH-III and electrophoresed on 691 NON-SDS gel, indicating that radioactivity peak appears at Rf value of 0.55. Gel filtration showed two radioactivity peaks equivalent to bound and free [3H]JH-III, respectively. JHBP was purified from hemolymph through gel filtration (Sephadex G-100), anion exchange chromatosraphv (DEAE Sepharose CL-6B), chromatofocusing chromatographv (PBE 94) and preparative electrophoresis.

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