• International Journal of Industrial Entomology and Biomaterials
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• 제6권2호
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• pp.115-123
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• 2003

Oviposition and egg laying is an important physiological and behavioural event in the life cycle of the silkworm, Bombyx mori (L). Oviposition and egg laying is dependent on a number of intrinsic and extrinsic factors viz., neural, hormonal, environmental, physical, behavioral etc for the perpetuation of population. Although, the virgin female moths have fully developed embryos but active egg laying begins under the influence of mating which provides essential copulation stimulus for oviposition. After mating drastic biochemical changes occurred that incites egg laying under the influence of optimum environmental conditions. Weight of pupae as well as larval density has significant role on oviposition and egg laying behaviour in the silkworm wherein high pupal weight and inadequate rearing space affects not only the biology, morphology and physiology but also the oviposition and egg laying. Surface topography, plane of inclination, mating length, age of male moth, temperature and photoperiod etc has significant effect on reproductive physiology of silkworm. An attempt has been made in this review article to elucidate briefly the works carried out on mating behaviour, direct and indirect copulation stimulus, vitellogenesis, influence of environmental factors besides effect of weight of pupae and or pharate adult and larval density on oviposition and egg laying behavior in the silkworm, B. mori and its significance in silkworm seed production.

• 한국응용곤충학회지
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• 제34권3호
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• pp.243-248
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• 1995

효과적인 미생물 제제로 사용할 수 있는 Bacillus thuringiensis 균주들을 분리하기 위하여 경기도 일원의 72군데의 저곡창고에서 먼지를 채취하여 B. thuringiensis의 분포와 독성을 조사하였다. 총 411개의 채취된 저곡창고의 먼지에서 포자와 내독소 단백질을 생산하는 146개에 이르는 많은 수의 B. thuringiensis가 분리되었다. 저곡창고에서 분리된 146개 Bt의 독성 분포는 나비목의 누에 (Bombyx mori)유충에 독성을 띠는 균주가 84%로 가장 많이 분리되었고, 파리목의 빨간 집모기(Culex pipiens)유충에는 3%, 나비목과 파리목에 동시에 독성을 띠는 균주는 1%였다. 그러나 딱정벌레목의 쌀바구미(Sitophitus oryzae)에 독성을 띠는 균주는 분리되지 않았으며, 내독소 단백질은 형성하지만 독성을 띠지 않는 B. thuringiensis가 12%의 비율로 분리되어 흔히 존재함을 알 수 있었다. 이상의 결과로서 저곡창고는 B. thuringiensis의 유리한 분리장소임을 알 수 있었다.

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2003년도 International Symposium of Silkworm/Insect Biotechnology and Annual Meeting of Korea Society of Sericultural Science
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• pp.78-79
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• 2003

To further understanding of the role of calreticulin in insects, we have isolated a cDNA of calreticulin from silkworm, Bombyx mori. The cDNA encodes a 398 amino acid residues of B. mori calreticulin with endoplasmic reticulum retentional HDEL motif at its C-terminus, and a predicted molecular mass of 45801 Da. The B. mori calreticulin shows high protein homology with those of G mellonella (88%), A. aegypti (71%) and H. sapiens (63%). (omitted)

• International Journal of Industrial Entomology and Biomaterials
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• 제8권1호
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• pp.95-99
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• 2004

Synthetic oligodeoxynucleotides (ODNs) containing unmethylated CpG dinucleotides in particular base contexts are known to induce immunity in vertebrate cells. In insect, however, it was recent to find out that ODNs induces insect immunity as other immune inducer such as lipopolysaccharide. However, the finding was solely based on one lepidopteran insect, Bombyx mori, and the expression of insect immunity was neither dependent on numbers of CpG repeats nor methylation of CpG repeats within ODNs. Instead, foreignness of DNA has been suggested to be a key factor governing induction of antibacterial peptide. In this study, we expanded our previous understanding to the potentiality of ODNs as an immune inducer for antifungal peptide in Galleria mellonella and B. mori. To do this, a defensin-type antifungal peptide gene, reported from G. mellonella was cloned and partially sequenced from G. mellonella and B. mori successfully and utilized as a probe in the Northern blot analysis. We found out that ODNs also work as an immune inducer for antifungal peptide in the fat body and midgut of G. mellonella and B. mori larvae. Also, induction pattern of antifungal peptide was irrelevant to the numbers of CpG repeats within ODNs as previously reported on the induction pattern of antibacterial peptides.

• International Journal of Industrial Entomology and Biomaterials
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• 제37권1호
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• pp.15-20
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• 2018

Most proteins produced in the endoplasmic reticulum (ER) of eukaryotic cells fold via disulfide formation (oxidative folding). Oxidative folding is catalyzed by protein disulfide isomerase (PDI) and PDI-related ER protein thiol disulfide oxidoreductases (ER oxidoreductases). In yeast and mammals, ER oxidoreductin-1s (ERO1s) supply oxidizing equivalent to the active centers of PDI. We previously identified and characterized the ERO1 of Bombyx mori (bERO1) as a thioredoxin-like protein that shares primary sequence homology with other ERO1s. Here we compare the reactivation of inactivated rRNase and sRNase by bERO1, and show that bERO1 and bPDI cooperatively refold denatured RNase A. This is the first result suggesting that bERO1 plays an essential role in ER quality control through the combined activities of bERO1 and bPDI as a catalyst of protein folding in the ER and sustaining cellular redox homeostasis.

• International Journal of Industrial Entomology and Biomaterials
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• 제29권2호
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• pp.145-152
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• 2014

The variation in the level of immune response related gene expression in silkworm, Bombyx mori following infection with Bombyx mori nucleopolyhedrovirus (BmNPV) was analyzed at different time intervals. The occlusion bodies of BmNPV orally inoculated to the two most divergent silkworm races viz., Sarupat (resistant to BmNPV infection) and CSR2 (susceptible to BmNPV infection) were subjected to oral BmNPV inoculation. The expression profile of gp 41 gene of BmNPV in the Sarupat and CSR2 races revealed that the virus could invade the midguts of both susceptible and resistant races. However, its multiplication was significantly less in the midgut of resistant race, while, in the susceptible race, the viral multiplication reached maximum level within 12 h. These findings indicate that potential host genes are involved in the inhibition of viral multiplication within larval midgut. The immune response genes arylphorin, cathepsin B, gloverin, lebocin, serpin, Hsp 19.9, Hsp 20.1, Hsp 20.4, Hsp 20.8, Hsp 21.4, Hsp 23.7, Hsp 40, Hsp 70, Hsp90 revealed differential level of expression on NPV infection. The gloverin, serpin, Hsp 23.7 and Hsp 40 genes are significantly up-regulated in the resistant race after NPV infection. The early up-regulation of these genes suggests that these genes could play an important role in baculovirus resistance in the silkworm, B. mori.

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2003년도 International Symposium of Silkworm/Insect Biotechnology and Annual Meeting of Korea Society of Sericultural Science
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• pp.53-54
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• 2003

The silk fibroin has been widely used as industrial materials such as cosmetics and food l)-2). In this study, general hydrolysate of B. mori fibroin as a biomaterial, development of functional-cometics included hydrolysate of B. mori fibroin will be discussed. (omitted)

• International Journal of Industrial Entomology and Biomaterials
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• 제25권2호
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• pp.205-208
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• 2012

Antheraea pernyi silkworm is a well known wild silkworm to produce a valuable silk fiber. A. pernyi silkworm was reared in Korean oak field and examined the cocoon characteristics, such as cocoon weight, cocoon shell weight, and percentage of cocoon shell weight. Degumming loss was also measured after alkali degumming process. A. pernyi silkworm spins tawny color cocoon in oval shape. Cocoon shell weight of A. pernyi silkworm, 0.78 g, was heavier than that of B. mori silkworm, 0.51 to 0.63 g. Cocoon shell percentage of A. pernyi silkworm, 32.8%, was higher than that of B. mori silkworm, 23.4 to 25.2%. Degumming loss percentage of A. pernyi silkworm, 17.1%, was lower than that of B. mori silkworm, 25.0%. SEM showed that the surface of the cocoon filament was coarse and oriented with longitudinal direction.

• International Journal of Industrial Entomology and Biomaterials
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• 제2권2호
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• pp.181-184
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• 2001

The effect of topical application with 10, 20 and 30 $\mu\textrm{g}$/ml cortisone and hydrocortisone to the fifth stadium larvae of the silkworm, B. mori on fat body glycogen, protein, total lipids, phospholipids, neutral lipids and haemolymph trehalose and protein has been studied. The fat body glycogen haemolymph trehalose significantly decreased in all the treated groups except in the 10 and 20 $\mu\textrm{g}$/ml treated groups. The fat body protein increased significantly in all the cortisone and hydrocortisone treated groups except in the group treated with 10 $\mu\textrm{g}$ hydrocortisone. Whereas that of haemolymph protein significantly increased in all the groups treated with cortisone and hydrocortisone. The total lipids, phospho1ipids and neutral lipids of the fat body decreased significantly in all the groups treated with cortisone and hydrocortisone when compared with that of carrier control.

• International Journal of Industrial Entomology and Biomaterials
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• 제9권2호
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• pp.265-267
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• 2004

The silkworm, Bombyx mori L. is prone to infection of various pathogenic organisms. Pebrine, one of the deadliest disease of silkworm caused by highly virulent parasitic microsporidian, Nosema bombycis has been understood since long. Infections of the disease range from chronic to highly virulent and can result in complete lose to the sericulture industry. Several strains and species of microsporidians have since been isolated from the infected silkworms. A new microsporidian spore was isolated from Lamerin breed of the silkworm B. mori have been studied under scanning electron microscope, found to be different in spore size (length 4.36$\pm$0.06 ${\mu}{\textrm}{m}$, width 2.14$\pm$0.01${\mu}{\textrm}{m}$) and shape (ova cylindrical with slight depression) from standard strain N. bombycis (length 3.08$\pm$0.21 ${\mu}{\textrm}{m}$, width 2.01$\pm$0.05 ${\mu}{\textrm}{m}$ and ovidal respectively). In immunological test, the silkworm breed Lamerin isolated micrisporidian spore does not react to different antibody (N. bombycis, M$_{11}$ and M$_{12}$) sensitized latex particle and thus appeared to be a different strain of microsporidian parasitic to the Lamerin breed of the silkworm B. mori.i.i.