• Journal of Microbiology and Biotechnology
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• 제34권6호
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• pp.1348-1355
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• 2024

The eukaryotic translation initiation factor eIF5B is a bacterial IF2 ortholog that plays an important role in ribosome joining and stabilization of the initiator tRNA on the AUG start codon during the initiation of translation. We identified the fluorophenyl oxazole derivative 2,2-dibromo-1-(2-(4-fluorophenyl)benzo[d]oxazol-5-yl)ethanone quinolinol as an inhibitor of fungal protein synthesis using an in vitro translation assay in a fungal system. Mutants resistant to this compound were isolated in Saccharomyces cerevisiae and were demonstrated to contain amino acid substitutions in eIF5B that conferred the resistance. These results suggest that eIF5B is a target of potential antifungal compound and that mutation of eIF5B can confer resistance. Subsequent identification of 16 other mutants revealed that primary mutations clustered mainly on domain 2 of eIF5B and secondarily mainly on domain 4. Domain 2 has been implicated in the interaction with the small ribosomal subunit during initiation of translation. The tested translation inhibitor could act by weakening the functional contact between eIF5B and the ribosome complex. This data provides the basis for the development of a new family of antifungals.

• 정보처리학회논문지B
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• 제19B권3호
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• pp.183-188
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• 2012

정보 검색에서 성능 저하의 주요 요인은 사용자의 질의와 검색 문서 사이에서의 어휘 불일치 때문이다. 어휘 불일치 문제를 해결하기 위해 본 논문에서는 어휘 번역확률을 이용한 번역기반 언어모델에 질의개념연관도를 반영한 검색 모델을 제안한다. 어휘관계 정보를 획득하기 위하여 문장-다음문장 쌍을 이용하여 어휘 번역확률을 계산하였다. 제안모델의 유효성을 검증하기 위해 TREC AP 컬렉션에 대해 실험하였다. 실험결과에서 제안모델이 언어모델에 비해 아주 우수한 성능향상을 보였고, 번역기반 언어모델에 비해서도 높은 성능을 나타냈다.

• Molecules and Cells
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• 제25권2호
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• pp.172-177
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• 2008

Eukaryotic translation initiation factor 5B (eIF5B) plays a role in recognition of the AUG codon in conjunction with translation factor eIF2, and promotes joining of the 60S ribosomal subunit. To see whether the eIF5B proteins of other organisms function in Saccharomyces cerevisiae, we cloned the corresponding genes from Oryza sativa, Arabidopsis thaliana, Aspergillus nidulans and Candida albican and expressed them under the control of the galactose-inducible GAL promoter in the $fun12{\Delta}$ strain of Saccharomyces cerevisiae. Expression of Candida albicans eIF5B complemented the slow-growth phenotype of the $fun12{\Delta}$ strain, but that of Aspergillus nidulance did not, despite the fact that its protein was expressed better than that of Candida albicans. The Arabidopsis thaliana protein was also not functional in Saccharomyces. These results reveal that the eIF5B in Candida albicans has a close functional relationship with that of Sacharomyces cerevisiae, as also shown by a phylogenetic analysis based on the amino acid sequences of the eIF5Bs.

• 한국어병학회지
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• 제34권1호
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• pp.17-22
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• 2021

Eukaryotic translation is initiated by either cap-dependent or cap-independent way, and the cap-independent translation can be initiated by the internal ribosomal entry site (IRES). In this study, to know whether the 5'UTR leader sequence of marine birnavirus (MABV) segment A and segment B can act as IRES, bicistronic vectors harboring a CMV promoter-driven red fluorescent gene (mCherry) and poliovirus IRES- or MABV's leader sequence-driven green fluorescent gene (eGFP) were constructed, then, transfected into a mammalian cell line (BHK-21 cells) and a fish cell line (CHSE-214 cells). The results showed that the poliovirus IRES worked well in BHK-21 cells, but did not work in CHSE-214 cells. In the evaluation of MABV's leader sequences, the reporter eGFP gene under the 5'UTR leader sequence of MABV's segment A was well-translated in CHSE-214 cells, indicating 5'UTR of MABV's segment A initiates translation in the cap-independent way and can be used as a fish-specific IRES system. However, the 5'UTR leader sequence of MABV's segment B did not initiate translation in CHSE-214 cells. As the precise mechanism of birnavirid IRES-mediated translation is not known, more elaborate investigations are needed to uncover why the leader sequence of segment B could not initiate translation in the present study. In addition, further studies on the host species range of MABV's segment A IRES and on the screening of other fish-specific IRESs are needed.

• 대한수학회보
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• 제61권3호
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• pp.585-595
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• 2024

We show that a pseudo-Anosov map constructed as a product of the large power of Dehn twists of two filling curves always has a geodesic axis on the curve graph of the surface. We also obtain estimates of the stable translation length of a pseudo-Anosov map, when two filling curves are replaced by multicurves. Three main applications of our theorem are the following: (a) determining which word realizes the minimal translation length on the curve graph within a specific class of words, (b) giving a new class of pseudo-Anosov maps optimizing the ratio of stable translation lengths on the curve graph to that on Teichmüller space, (c) giving a partial answer of how much power is needed for Dehn twists to generate right-angled Artin subgroup of the mapping class group.

• Korean Journal of Mathematics
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• 제22권4호
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• pp.711-723
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• 2014

We prove in this paper the absolute continuity of the representing measures of the hypergeometric translation operators $\mathcal{T}_x$ and $\mathcal{T}_x^W$ associated respectively to the Cherednik operators and the Heckman-Opdam theory attached to the root system of type $B_2$ and $C_2$ which are studied in [9].

• 한국수학교육학회지시리즈B:순수및응용수학
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• 제28권1호
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• pp.43-53
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• 2021

We study translation surfaces in the Euclidean 3-space ��3 and the Gauss map N with respect to the so-called Cheng-Yau operator ☐. As a result, we prove that the only translation surfaces with Gauss map N satisfying ☐N = AN for some 3 × 3 matrix A are the flat ones. We also show that the only translation surfaces with Gauss map N satisfying ☐N = AN for some nonzero 3 × 3 matrix A are the cylindrical surfaces.

• 미생물학회지
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• 제40권1호
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• pp.7-11
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• 2004

eIF5B는 단백질합성의 개시 인자로서 Met-$tRNA^{Met}$을 AUG 개시코돈에 전달하고, 리보솜의 두 소단위체 결합을 유도한다. 이 인자의 기능을 연구할 목적으로 eIF5B를 코딩하는 FUN12 유전자의 5'말단 부위를 삭제하는 연구를 수행하였다. 프로모터의 대부분을 삭제한 FUN12를 함유한 pRS효모벡터를 FUN12가 삭제되어 천천히 자라는 돌연변이주 ($fun12{\Delta}g$)에 전달하였을 때 그 표현형을 부분적으로 상보하였다. 위와 같이 제조된 벡터에서 N-말단이 상실된 eIF5B 단백질이 발현되었고, 그 양이 정상 균주에서 발현되는 eIF5B 양의 약 5%에 불과하였다. 이와 같이 부분적으로 성장을 상보한 균주에서 발현된 적은 양의 단백질합성개시 인자 eIF5B는 직접적으로 그 성장을 제한하는 요소로 작용한다. 이러한 균주에서 성장의 제한인자인 eIF5B는 in vitro 에서도 역시 전체 단백질 합성의 활성을 조절하였다.

• 대한수학회보
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• 제59권5호
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• pp.1093-1103
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• 2022

In this paper, we establish an evaluation formula to calculate the Wiener integral of polynomials in terms of natural dual pairings on abstract Wiener spaces (H, B, 𝜈). To do this we first derive a translation theorem for the Wiener integral of functionals associated with operators in 𝓛(B), the Banach space of bounded linear operators from B to itself. We then apply the translation theorem to establish an integration by parts formula for the Wiener integral of functionals combined with operators in 𝓛(B). We finally apply this parts formula to evaluate the Wiener integral of certain polynomials in terms of natural dual pairings.

• 정보처리학회논문지B
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• 제17B권6호
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• pp.459-466
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• 2010

자연언어처리는 여러 가지 모호성 문제를 가지는데, 특히 영한기계번역은 번역 과정의 각 단계마다 해결해야 할 모호성 문제를 가진다. 본 논문에서는 실용적인 영한기계번역 시스템의 개발을 목적으로 영어 분석의 효율성을 높이기 위해 영어 단어의 품사 모호성 해소 문제에 초점을 두었다. 기계번역의 효율성 제고를 위해 영한기계번역 시스템에 통합하기 위한 품사결정 모듈은 빠른 시간에 정확한 품사결정을 하면서도 오류를 최소화 하여야 한다. 본 논문에서는 확률적 품사결정 방법을 제안하고 3가지 품사결정 확률 모델을 제시하였다. Penn Treebank 말뭉치로부터의 통계 정보를 이용하여 확률 모델을 구축하였으며 실험을 통해 제안한 품사결정 방법의 정확성과 품사결정에 의한 기계번역 시스템의 효율 향상 정도를 제시하였다.