• Korean Journal of Computational Design and Engineering
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• v.1 no.2
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• pp.122-132
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• 1996

Features are generic shapes with which engineers associate certain attributes and knowledge useful in reasoning about the product. Feature-based modeling systems support additional levels of information beyond those available in geometric modelers. The objective of this study is to develop a PC level feature-based modeling system which explicitly represents dimensions of the part. The feature-based modeler retains all the benefits of traditional B-rep. solid models, and represents the dimensions at a high level of a abstraction so that dimension driven geometry can be achieved.

• Journal of the Korean Society for Precision Engineering
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• v.10 no.1
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• pp.89-99
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• 1993

A solid modeller was developed in this paper. Representation scheme of modelling is nonmanifold B-rep. While namy B-rep modellers use edges as basic element of modelling, this modeller is triangle-based. New modelling element, split triangle that is composed of 3 pure half-edges and has no information about the adjoining triangles, was defined to enlarge the range of representation. Corresponding algorithms for Boolean set operations were also developed.

• Journal of Microbiology and Biotechnology
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• v.29 no.3
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• pp.482-488
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• 2019

Porcine circovirus type 2 (PCV2) is the causative agent of postweaning multisystemic wasting syndrome (PMWS) in pigs. Replicase (Rep) proteins are considered essential for viral replication. Capsid (Cap) protein is the primary immunogenic protein that induces protective immunity. Little is known about comparison on the immunogenicity of PCV2 Rep and Cap fusion protein and Cap protein. In the present study, recombinant baculoviruses expressing the Rep-Cap fusion protein (Bac-Rep-Cap) and the Cap protein (Bac-Cap) of PCV2 were constructed and confirmed with western blot and indirect fluorescence assay. Immunogenicities of the two recombinant proteins were tested in mice. The titers of antibodies were determined with a PCV2-specific enzyme-linked immunosorbent assay (ELISA) and a serum neutralization assay. The $IFN-{\gamma}$ response of immunized mice was measured by ELISA. The mice immunized with the Bac-Rep-Cap and Bac-Cap successfully produced Cap-specific immunoreaction. The mice immunized with the Bac-Cap developed higher PCV2-specific neutralizing antibody titers than mice injected with the Bac-Rep-Cap. $IFN-{\gamma}$ in the Bac-Rep-Cap group was increased compared to those in the Bac-Cap group. Vaccination of mice with the Bac-Rep-Cap showed significantly decreased protective efficacy compared to the Bac-Cap. Our findings will indubitably not only lead to a better understanding of the immunogenicity of PCV2, but also improved vaccines.

• Spatial Information Research
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• v.19 no.1
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• pp.93-105
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• 2011

In recent years, large and complex three-dimensional building has been constructed by the development of building technology and advanced IT skills, and people have lived there and spent a considerable time so far. Accordingly. in this sophisticatcd three-dimensional space, emergencies services or convenient information services have been in demand. In order to provide these services efficiently, understanding of topological relationships among the complex space should be supported naturally. Not on1y each method of understanding the topological relationships but also its efficiency can be different depending on different topological data models. B-rep based data model is the most widely used for storaging and representing of topological relationships. And from early 2000s, many researches on a network based topological data model have been conducted. The purpose of this study is to verify the efficiency of performance on spatial queries. As a result, Network-based topological data model is more efficient than B-rep based data model for determining the spatial relationship.

• Korean Journal of Computational Design and Engineering
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• v.11 no.4
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• pp.289-302
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• 2006

It is becoming a common trend that many designers work on a very complex assembly together in a collaborative environment. In this environment, every designer should be able to see the whole assembly in a full detail or in a rough shape at least. Even though the hardware technology is being improved very rapidly, it is very difficult to display a very complex assembly at a speed to allow smooth interactions for designers. This problem could be solved if a designer could manipulate his portion of the assembly in a full resolution while the remaining portion of the assembly is displayed in a rough resolution. It is also desired that the remaining portion is converted to the full resolution when needed. To realize this environment, the capabilities to simplify the portions of an assembly and to reset to the original resolution should be added to the current CAD systems. Thus operators realizing multi-resolution on B-rep are proposed in this paper. They are: wrap-around, smooth-out, and thinning operator. Through appropriately applying these operators sequentially, an assembly model of any desired resolution can be easily generated. Of course, the assembly can go back to the finer resolution. In this paper, the data structures and the processes to realize these operators are described and a prototype modeling system with these operators is also demonstrated.

• Korean Journal of Computational Design and Engineering
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• v.6 no.1
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• pp.48-58
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• 2001

One of the biggest problems of rapid prototyping(RP) technologies lies in their standard file format for CAD data exchange. Current methods using the de facto industry standard 'STL'have at times resulted in problems such as accuracy, redundancy, and integrity. In this paper we propose a STEP based data exchange framework for rapid prototyping systems. In this paradigm of data exchange, STEP models can be imported and converted into faceted B-rep. solid models for visualization and 2-D layer data for RP. Also an STL model, on the other hand, can be converted into a faceted B-rep. STEP model and exported as a new data exchange model with RP information.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2002.05a
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• pp.836-839
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• 2002

This paper describes the selective Boolean operations on non-manifold geometric models whose union and subtraction operations are communicative. These operations guarantee the same resulting shape in spite of change of the order of Boolean operations, and the integrity of the model for omission of some features. In addition, a B-rep model for a modified modeling history is obtained in a short time, as no boundary evaluation is necessary. These features enable easy implementation of multi-resolution representation of B-rep models.

• Journal of Mechanical Science and Technology
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• v.19 no.2
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• pp.558-566
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• 2005

We propose a feature-based multi-resolution representation of B-rep solid models using history-based Boolean operations based on the merge-and-select algorithm. Because union and subtraction are commutative in the history-based Boolean operations, the integrity of the models at various levels of detail (LOD) is guaranteed for the reordered features regardless of whether the features are subtractive or additive. The multi-resolution solid representation proposed in this paper includes a non-manifold topological merged-set model of all feature primitives as well as a feature-modeling tree reordered consistently with a given LOD criterion. As a result, a B-rep solid model for a given LOD can be provided quickly, because the boundary of the model is evaluated without any geometric calculation and extracted from the merged set by selecting the entities contributing to the LOD model shape.

• Korean Journal of Clinical Laboratory Science
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• v.36 no.1
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• pp.1-6
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• 2004

The enterobacterial repetitive intergenic consensus (ERIC)-PCR is a recently described DNA fingerprinting technique based on amplification of repetitive element distributed in bacteria. We applied of ERIC-PCR to clinical isolates of Vibrio parahaemolyticus and other bacteria associated diarrhea. Twenty isolates of V. parahaemolyticus were used for intragenic genotyping, which were isolated from 2001 to 2002 in Chungbuk National University hospital. For interspecies genotyping, V. vulnificus, V. alginolyticus, V. parahaemolyticus, Escherichia coli, Salmonella and Shigella spp. were used. The genotyping were analyzed by ERIC-PCR. The genotyping of V. parahaemolyticus were grouped two major pattern (A, B) and were subdivided into 10 subtypes (A1, A2, B1-B8) by ERIC-PCR. These method distinctly differentiated bacterial species associated diarrhea. Those results show that ERIC-PCR can be reliable and efficient method for genotyping of V. parahaemolyticus and bacteria associated diarrhea.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.135.2-136
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• 2003

To characterize plasmids in Xanthomonu axonopodis pv. glycines, we isolated plasmids pAG1 from the strain AG1 and pXAG81 and PXAG82 from the strain Bra, respectively, and sequenced three plasmids. The size of plasmids, pAG1, pXAG81, and pXAG82 was 15,149-base pairs (bp), 26,727-bp, and 1,496-bp, respectively Fifteen and twenty six possible open reading frames (ORFs) were present in pAG1 and pXAG81, respectively. Only one ORF homologous to a rep gene of Xylella fastidiosa was present in pXAG82. pAG1 contained genes homologous to avrBs3, tnpA, tnpR, repA, htrA, three parA genes, M.XmaI, R.XmaI, and six hypothetical proteins. pXAG81 contained genes homologous to avrBs3, tnpA, tnpR, repA, htrA, two parA genes, pemI, pemK, mobA, mobB, mobC, mobD, mobE, trwB, traF, traH, ISxac2, and eleven hypothetical proteins. Based on DNA sequence analysis, we presume that pXAG81 is a conjugal plasmid. Interestingly, we found 0.5-kb truncated avirulence gene similar to aurXacE3 on the right border of avrBs3 homolgs of pAG1 and pXAG81. Two hundred twenty five isolates were analyzed to find aurBS3 or tra gene homologs by Southern hybridization. The numbers of avrBs3 homolog varied from 3 in AG1 to 8 in AG166. Two hundred seventeen isolates appeared to can conjugative plasmids (pXAG81 type), and thirty eight isolates appeared to carry non-conjugative plamids (pAGl type). This indicated that aurBs3 gene homologs might be spread by conjugation in X. axonopodis pv. glycines.