• 한국환경과학회지
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• 제16권4호
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• pp.487-493
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• 2007

In this study is analysis which dams breach shapes are effect on peak discharge of dam-failure. The dam breach shapes and failure time are important peak discharge when dam failure. When dam failure times are 1hr, 2hr and 3hr condition for the ECRD and 0.1hr and 0.2hr for the CG and CFRD that breach shapes changed base length $B_b=1Hd,\;B_b=2Hd\;and\;B_b=3Hd$. As the results from DAMBRK(Dam Break model) peak discharge are increase base widths lengthen. As failure time is longer then peak discharge is decrease. So peak discharge is increase more short of dam failure time. Also peak discharge is increase become larger dam breach shapes.

• Asian Pacific Journal of Cancer Prevention
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• 제15권2호
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• pp.861-865
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• 2014

Background: High risk HPV (HR-HPV) testing has been recommended as an effective tool along with cytology screening in identification of cervical intraepithelial lesions (CINs) and prevention of their progress towards invasive cervical cancer. The aim of this study was to assess the HR-HPV DNA status by Hybrid Capture 2 (HC2) assay in healthy asymptomatic women of North-East India. Materials and Methods: This study examined cervical cell samples of forty three (n=43) healthy women by HC2 assay. A High Risk HPV DNA kit (Qiagen) was used which can detect 13 high risk HPV types: 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 and 68. Results: The mean relative light units (RLU) for samples was in the range of 141-5, 94, 619. HR-HPV DNA was confirmed in 16% (7/43) of participant women samples. Among demographic and clinical parameters, menstrual irregularity (p=0.039) and infection history (p=0.028) has shown statistically significant differences between the HR-HPV-positive and negative groups. In the HR-HPV positive group, two women were confirmed for CINs after colposcopy and histopathologic examination. Conclusions: We suggest that there may be an association between irregular menstruation and infection history of the urogenital tract with HR-HPV DNA prevalence in North-East Indian asymptomatic women. HC2 assay can be a valuable tool for HR-HPV screening.

• The Korean Journal of Physiology
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• 제5권2호
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• pp.29-40
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• 1971

Oxygen consumption, pulmonary ventilation, heart rate, and breathing frequency were measured on 8 men walking on a treadmill carrying load of 9 kg on hand, back, or head. Besides measurements were made on subjects carrying loads of 2.6 kg each on both feet. The speed of level walking was 4, 5, and 5.5km/hr and a fixed speed off km/hr with grades of 0, 3, 6, and 9%. Comparisons were made between free walking without load and walking with various types of loads. The following results were obtained. 1. In level or uphill walking the changes in oxygen consumption, pulmonary ventilation, breathing frequency and heart rate were smallest in back load walking, and largest in hand load walking. The method of back load was most efficient and hand load was the least efficient. The energy cost in head load walking was smaller than that of in hand load walking. It was assumed that foot load costed more energy than hand load. 2. In level walking the measured parameters increased abruptly at the speed of 5.5 km/hr. Oxygen consumption in a free walking at 4 km/hr was 11.4ml/kg b.wt., and 13.1 ml/kg b.wt. 5.5 km/hr, and in a hand load walking at 4 km/hr was 13.9, and 18.8 ml/kg b. wt. at 5.5 km/hr. 3. In uphill walking oxygen consumption and other parameters increased abruptly at the grade of 6%. Oxygen consumption at 4 km/hr and 0% grade was 11.4 ml/kg b. wt., 13.6 at 6% grade, and 16.21/kg b. wt. at 9% grade in a free walking. In back load walking oxygen consumption at 4km/hr and 0% grade was 12.3 ml/kg b.wt.,14.9 at 6% grade, and 18.7 ml/kg b.wt. In hand load walking the oxygen consumption was the greatest, namely, 13.9 at 0% grade, 17.9 at 6%, and 20.0 ml/kg b. wt. at 9% grade. 4. Both in level and uphill walking the changes in pulmonary ventilation and heart rate paralleled with oxygen consumption. 5. The changes in heart rate and breathing frequency in hand load were characteristic. Both in level and uphill walk breathing frequency increased to 30 per minute when a load was held on hand and showed a small increase as the exercise became severe. In the other method of load carrying the Peak value of breathing frequency was less than 30 Per minute. Heart rate showed 106 beats/minute even at a speed of 4 km/hr when a load was held on hand, whereas, heart rate was between, 53 and 100 beats/minute in the other types of load carriage. 6. Number of strides per minute in level walking increased as the speed increased. At the speed floater than 5 km/hr number of strides per minute of load carrying walk was greater than that of free walking. In uphill walk number of strides per minute decreased as the grade increased. Number of strides in hand load walk was greatest and back load walk showed the same number of strides as the free walk.

• 대한본초학회지
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• 제28권3호
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• pp.69-74
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• 2013

Objectives : Since hypopigmentation is known to increase the risk of skin cancer, melanogenesis in the skin needs to be regulated. Here, we evaluated the melanogenesis stimulatory effects of a modified Goagium herbal remedy (HR) and HR+ox bile (Bos taurus domesticus) extract (OBE) to address hypopigmentation disorders. Methods : B16F10 melanoma cells were treated with different dosages of HR and HR+OBE for 24 to 48 h after 1 h of 10 nM ${\alpha}$-melanocyte stimulating hormone (${\alpha}$-MSH). After the treatment, cell viability, tyrosinase activity, melanin synthesis and the expression of genes related to melanin synthesis were measured and the regulation of the ${\alpha}$-MSH signalling through cAMP responding element binding protein (CREB) was determined. Results : HR and HR+OBE with the ranges of $15{\sim}100{\mu}g/mL$ did not affect cell viability in melanoma cells. The 1 h treatment of HR+OBE (50 and $100{\mu}g/mL$) potentiated the phosphorylation of CREB by enhancing ${\alpha}$-MSH signaling and its 24 h treatment increased CREB expression. Consistent with CREB potentiation, their treatment for 24 h, the expression of microphthalmia-associated transcription factor (MIFT), tyrosinase, tyrosinase related protein (TRP)-1 and TRP-2 were increased in realtime PCR. Ultimately, the 48 h treatment of HR+OBE (50 and $100{\mu}g/mL$) increased tyrosniase activity and melanin contents in the melanoma cells in comparison to the control. Conclusions : HR+OBE (50 and $100{\mu}g/mL$) increases melanin synthesis in B16F10 melanoma cells via the stimulation of tyrosinase activity and expression of MIFT, tyrosinase, TRP-1 and TRP-2. HR+OBE can be used as the a possible treatment for hypopigmentation of the skin.

• 한국광학회지
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• 제4권4호
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• pp.487-492
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• 1993

편광유지 광섬유를 이용하여 자이로스코프를 구성하였다. 전체 시스템의 총 광 손실은 약 25dB였으며, 지구자전속도를 기준으로 하여 장기 drift와 단기 잡음을 측정하였다. 장기 drift는 온도변화와 밀접한 관계가 있었으며 약 $3^{\circ}/hr$ 정도의 값을 얻었고, 단기 근 잡음은 약 $0.6^{\circ}/hr/{\sqrt{Hz}}$의 값을 나타내었다.

• Tuberculosis and Respiratory Diseases
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• 제43권4호
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• pp.579-589
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• 1996

연구배경 : 패혈증에서 백혈구의 활성화는 미생물 및 숙주 독성 물질의 청소에 중요한 역활을 하며 한편으로 활성화된 호중구와 그의 특성 물질은 조직 손상파 장기의 기증 부전을 초래한다. 백혈구의 CD11/18 유착 분자는 감염 및 염증 부위로 호중구 이동의 첫 단계인 호중구-내피 세포 유착을 조절한다. 패혈증 치료 전락으로 호중구 억제 효과의 가능성을 알아보기 위해 rat 에서 Escherichia coli 폐렴을 유발하여 CD11b에 대한 단클론 항체(MAb 1B6)의 효과를 평가하였다. 방법 : 1 mg/kg CD11b에 대한 단클론 항체와 1 mg/kg BSA출 폐렴 유발 6 시간전, 유발시 및 유발 6 시간후 무작위적으로 피하 투여하였다. 폐렴 유발후 무작위적으로 24, 60 및 90%의 산소를 투여하였으며 폐렴 유발후 4시간 및 3일간 하루에 한번씩 100 mg/kg의 ceftriaxone을 투여하였다. 말초 및 폐포 호중구와 폐 손상의 지표인 D(A-a)O2, W/D LW ratio 및 폐포 세척액 단백 농도를 12시간과 96시간까지 생존한 동물에서 측정하였다. 결과 : CD11b에 대한 단클론 항체는 말초 및 폐포 호중구를 감소시켰으며 96시간 보다 12시간에 더욱 감소시켰다. 폐손상 지표는 단클론 항체 투여군과 대조군을 비교시 차이를 보이지 앉았으나 생존 동물 수의 부족으로 통계학적 평가의 의미가 없었다. 조기(6 시간) 사망률은 항체 투여군 51%로 대조군 31%보다 의미(P=0.02) 있게 증가하였으나 후기 사망률(12 시간에서 72 시간)은 항체 투여군 44% 대조군 36%로 의미(P=0.089) 있는 차이가 없었다. 결론 : CD11b/18 유착 분자는 감염 및 염증 부위로 호중구의 이동을 조절하는 것으로 알려져 있다. CD11b에 대한 단클론 항체는 rat의 폐인성 패혈증에서 폐포 호중구를 감소시키고 조기 사망률을 증가시켰다. 따라서 CD11b에 대한 단클론 항체가 rat의 폐인성 패혈증의 초기에 호중구의 폐포내 이동을 억제시켜 숙주의 방어 기전에 장애를 초래하여 조기 사망률을 증가시키는 것으로 사료된다.

• Toxicological Research
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• 제11권1호
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• pp.1-8
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• 1995

Fumonisins are a family of mycotoxins produced by the fungus Fusarium moniliforme which is a common contaminant in corn. Fumonisins are potent inhibitors of sphingosine and sphinganine N-acyltransferase (ceramide synthase), key enzymes in sphingolipid metabolism. The purpose of this study was to provide the evidence that the elevated levels of free sphingoid bases (primarily sphinganine) and depletion of complex sphingolipids were closely related to the inhibition of cell growth in LLC-$PK_1$ cells exposed to fumonisin $B_1$$(\leq 35 {\mu}M)$. Concentrations of fumonisin $B_1$ between 10 and $35 {\mu}M$ were known to inhibit cell growth without cytotoxicity in $LLC-PK_1$ cells (Yoo et al. Toxicol. Appl. Pharmacol. 114, 9-15, 1992). Cells exposed to 35$\mu M$ fumonisin B$_1$ for 48 and 72 hr developed a fibroblast-like (elongated and spindle-shaped) appearance and were less confluent than normal cells. At between 24 and 48 hr after exposure to fumonisin $B_1$ cells were beginning to show the inhibition of cell growth and at 72 hr the number of viable cells in fumonisin-treated cultures was about 50% of concurrent control cultures. During the 24 hr lag period preceding inhibition of cell growth, the free sphinganine levels in cells exposed to $35 {\mu}M$ fumonisin $B_1$ were highly elevated (approximately 230 fold higher than normal cells). The elevated levels of free sphinganine were $435\pm14$$pmoles/{10^6}$ cells at 48 hr and approximately TEX>$333\pm11$$pmoles/{10^6}$ cells in cells exposed to $35{\mu}M$ fumonisin$B_1$ at 72 hr, while the levels of free sphinganine in normal cells were less than 2$pmoles/{10^6}$ cells. Under the same condition, depletion of intracellular complex sphingolipids as a consequence of fumonisin inhibition of de novo sphingolipid biosynthesis and turnover pathway was appeared. Content of free sphingold bases in dividing cells was more elevated than in confluent cells at 24-48 hr after cells were exposed to $20{\mu}M$ fumonisin $B_1$. The dividing cells were showing the inhibition of cell growth at 48-72 hr and $20{\mu}M$ fumonisin $B_1$. The results of this study support the hypothesis that the inhibition of cell growth is very well related to the disruption of sphingolipid metabolism in $LLC-PK_1$ cells.

• 한국방사선학회논문지
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• 제17권1호
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• pp.53-62
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• 2023

Lower Extremity CT Angiography 검사 시 환자의 심박동 수(HR)에 따라 하지 혈관의 조영증강 차이를 비교 분석하여 최적화된 영상 기법을 찾고 유용성을 평가하고자 한다. 2022년 1월부터 22년 8월까지 아주대 병원을 내원하여 Lower Extremity CT Angiography 검사를 진행한 외래환자 139명을 대상으로 하였으며, 심박동 수(HR)에 따라 A 그룹(HR ≤65), B 그룹(65<HR<80), C 그룹(80≤ HR)으로 나누었다. 추가로 지연시간을 설정하여 D 그룹(HR ≤65), E 그룹(65<HR<80), F 그룹(80≤ HR) 으로 나누어 조영제 도달시간과 조영증강 평균값을 비교 분석하였다. 결과적으로 심박동 수(HR)의 차이는 하지 혈관 조영증강 강도에 영향을 주었고, 그룹별 정량적 평가 결과 Heart Rate가 65회 이상인 B, C 그룹은 A 그룹에 비해 슬와 동맥의 HU 값이 우수하였다(p<0.001^***). 지연시간을 적용한 D 그룹은 A 그룹에 비하여 하지 동맥의 조영증강 효과가 개선이 되었음을 확인할 수 있었고(p<0.001^***), 다른 그룹과의 비교한 분석은 유의미하지 않았다. 심박동 수(HR) 활용한 Lower extremity CTA 검사 방법은 유의미 하였고, 특히 심박동 수(HR)가 65이하로 낮은 경우에는 환자에게 적절한 지연시간을 적용하여 검사하는 것이 도움이 된다

• Journal of Pharmaceutical Investigation
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• 제23권2호
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• pp.111-118
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• 1993

The hemolytic characteristics of amphotericin B-containing liposomes have been investigated in vitro. From the hemolysis of human erythrocytes against free and liposomal amphotericin B, the marked reduction in the toxicity of amphotericin B was observed by incorporating the drug in egg PC liposomes. For 45 min, free amphotericin B at $9.6\;{\mu}g/ml$ could completely lyse 2 wt% human erythrocytes. However, liposomal amphotericin B had essentially no lytic effect even in the range over $9.6\;{\mu}g/ml$. In the 66 hr-hemolysis experiment, liposomal amphotericin B showed the slowly hemolysing chracteristics during the experimental period regardless of the concentration of amphotericin B but rapid hemolysis only for 12 hr was observed in the case of free amphotericin B and the degree of hemolysis for 12 hr was maintained after that time. Also the hemolysing ability of liposomal amphotericin B at $4\;{\mu}g/ml$ was lower than that of free amphotericin B at the same concentration for 66 hr. On the other hand, the dependence of hemolysis on amphotericin B contents in egg PC liposomes was significant between 1.64 mole% amphotericin B-containing liposomes and 15.79 or 27.27 mole% amphotericin B-containing liposomes. But no marked difference in hemolysis was observed between 15.79 and 27.27 mole% amphotericin B-containing liposomes. Especially, cholesterol as an excipient in amphotericin B-containing liposomes significantly reduced the hemolysis of human erythrocyte. The degree of hemolysis in 5 mole% amphotericin B-containing liposomes was reduced to approximately 50% of value in the cholesterol-free liposomes by adding 50% cholesterol.

• 한국식품조리과학회지
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• 제16권1호
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• pp.75-83
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• 2000

The effects of salting methods on sensory and microbiological properties of Kakdugi were evaluated during fermentation at 10$\^{C}$ for up to 52 days. Kakdugi samples were prepared by 4 different salting methods at the final salt concentration about 1.5%, which was appropriate for organoleptic quality. The salting methods for radish cubes(2cm size) of Kakdugi included; 1) Treatment S-1: spraying dry salt uniformly onto the radish cubes, at the concentration of about 1.5%(w/w) and cured for 1 hr, 2) Treatment S-5: spraying dry salt uniformly onto the radish cubes, at the concentration of about 1.2%(w/w) and cured for 5 hr, 3) Treatment B-1: brining radish cubes in a 8.5%(w/v) salt solution and cured for 1 hr, 4) Treatment B-5: brining radish cubes in a 4.0%(w/v) salt solution and cured for 5 hr. During the early stage of fermentation, sensory test showed higher scores in the overall acceptability of Kakdugi prepared with salting methods S-1 and S-5 than those with B-1 and B-5. However, the trend of acceptability has been reversed by the treatments B-1 and, more notably, by B-5 at the later stage of fermentation. The counts of lactic acid bacteria increased remarkably and then decreased gradually after the optimum ripening period. The major lactic acid bacteria isolated and identified from Kakdugi were Pediococcus, Streptococcus, Leuconostoc, and Lactobacillus.