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http://dx.doi.org/10.7314/APJCP.2014.15.2.861

Hybrid Capture 2 Assay Based Evaluation of High-Risk HPV Status in Healthy Women of North-East India  

Rai, Avdhesh Kumar (DBT Centre for Molecular Biology and Cancer Research, Dr. B. Borooah Cancer Institute)
Das, Debabrata (DBT Centre for Molecular Biology and Cancer Research, Dr. B. Borooah Cancer Institute)
Kataki, Amal Chandra (Department of Gynaecologic Oncology, Dr. B. Borooah Cancer Institute)
Barmon, Debabrata (Department of Gynaecologic Oncology, Dr. B. Borooah Cancer Institute)
Aggarwal, Dippy (Department of Obstetrics and Gynaecology, Hayat Multi-Speciality Hospital)
Deka, Pankaj (Department of Gynaecologic Oncology, Dr. B. Borooah Cancer Institute)
Shrivastava, Sushruta (Department of Gynaecologic Oncology, Dr. B. Borooah Cancer Institute)
Sharma, Jagannath Dev (Department of Pathology, Dr. B. Borooah Cancer Institute)
Sarma, Anupam (Department of Pathology, Dr. B. Borooah Cancer Institute)
Baruah, Upasana (Department of Gynaecologic Oncology, Dr. B. Borooah Cancer Institute)
Sharma, Mousumi (Department of Pathology, Dr. B. Borooah Cancer Institute)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.2, 2014 , pp. 861-865 More about this Journal
Abstract
Background: High risk HPV (HR-HPV) testing has been recommended as an effective tool along with cytology screening in identification of cervical intraepithelial lesions (CINs) and prevention of their progress towards invasive cervical cancer. The aim of this study was to assess the HR-HPV DNA status by Hybrid Capture 2 (HC2) assay in healthy asymptomatic women of North-East India. Materials and Methods: This study examined cervical cell samples of forty three (n=43) healthy women by HC2 assay. A High Risk HPV DNA kit (Qiagen) was used which can detect 13 high risk HPV types: 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 and 68. Results: The mean relative light units (RLU) for samples was in the range of 141-5, 94, 619. HR-HPV DNA was confirmed in 16% (7/43) of participant women samples. Among demographic and clinical parameters, menstrual irregularity (p=0.039) and infection history (p=0.028) has shown statistically significant differences between the HR-HPV-positive and negative groups. In the HR-HPV positive group, two women were confirmed for CINs after colposcopy and histopathologic examination. Conclusions: We suggest that there may be an association between irregular menstruation and infection history of the urogenital tract with HR-HPV DNA prevalence in North-East Indian asymptomatic women. HC2 assay can be a valuable tool for HR-HPV screening.
Keywords
HC2 assay; high risk HPV; healthy women of North East India;
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1 Schiffman M, Wentzensen N, Wacholder S, et al (2011). Human papillomavirus testing in the prevention of cervical cancer. J Natl Cancer Inst, 103, 368-83   DOI
2 Sarian LO, Derchain S, Shabalova I, et al (2010). Optional screening strategies for cervical cancer using standalone tests and their combinations among low- and medium-income populations in Latin America and Eastern Europe. J Med Screen, 17, 195-203   DOI
3 Sowjanya AP, Jain M, Poli UR, et al (2005). Prevalence and distribution of high-risk human papilloma virus (HPV) types in invasive squamous cell carcinoma of the cervix and in normal women in Andhra Pradesh, India. BMC Infect Dis, 5, 116   DOI   ScienceOn
4 Sowjanya AP, Paul P, Vedantham H, et al (2009). Suitability of self-collected vaginal samples for cervical cancer screening in periurban villages in Andhra Pradesh, India. Cancer Epidemiol Biomarkers Prev, 18, 1373-8   DOI
5 Tao K, Yan J, Yang H, et al (2013). Comparative study of the Cervista and Hybrid Capture 2 methods in detecting high-risk human papillomavirus in cervical lesions. Diagn. Cytopathol. [Epub ahead of print???]
6 Three-Year Report of Population Based Cancer Registries 2009-2011: Incidence and Distribution of Cancer. (2013). NCDIR-NCRP (ICMR), Bangalore.
7 Zhao C, Weng B, Li Z, Yang H, Austin RM (2013). Follow-up outcomes of a large cohort of low-risk women with negative imaged liquid-based cytology and negativeHPV test results. Am J Clin Pathol, 139, 32-8   DOI
8 Leinonen M, Nieminen P, Kotaniemi-Talonen L, et al (2009). Age-specific evaluation of primary human papillomavirus screening vs conventional cytology in a randomized setting. J Natl Cancer Inst, 101, 1612-23   DOI   ScienceOn
9 Jentschke M, Soergel P, Hillemanns P (2013). Evaluation of a multiplex real time PCR assay for the detection of human papillomavirus infections on self-collected cervicovaginal lavage samples. J Virol Methods, 193, 131-4   DOI
10 Katyal S, Mehrotra R (2011). Complementary procedures in cervical cancer screening in low resource settings. J Obstet Gynaecol India, 61, 436-8   DOI
11 Kitchener HC, Almonte M, Thomson C, et al (2009). HPV testing in combination with liquid-based cytology in primary cervical screening (ARTISTIC): a randomised controlled trial. Lancet Oncol, 10, 672-82   DOI   ScienceOn
12 Meijer CJ, Berkhof J, Castle PE, et al (2009). Guidelines for human papillomavirus DNA test requirements for primary cervical cancer screening in women 30 years and older. Int J Cancer, 124, 516-20   DOI   ScienceOn
13 Pandey S, Mishra M, Chandrawati (2012). Human papillomavirus screening in north Indian women. Asian Pac J Cancer Prev, 13, 2643-6   과학기술학회마을   DOI   ScienceOn
14 Brun-Micaleff E, Coffy A, Rey V, et al (2013). Cervical cancer screening by cytology and human papillomavirus testing during pregnancy in French women with poor adhesion to regular cervical screening. J Med Virol, 86, 536-45.
15 Piyathilake CJ, Badiga S, Paul P, et al (2010). Indian women with higher serum concentrations of folate and vitamin B12 are significantly less likely to be infected with carcinogenic or high-risk (HR) types of human papillomaviruses (HPVs). Int J Womens Health, 2, 7-12
16 Ronco G, Giorgi-Rossi P, Carozzi F, et al (2008). Results at recruitment from a randomized controlled trial comparing human papillomavirus testing alone with conventional cytology as the primary cervical cancer screening test. J Natl Cancer Inst, 100, 492-501   DOI
17 Das D, Rai AK, Kataki AC, et al (2013). Nested multiplex PCR based detection of human papillomavirus in cervical carcinoma patients of North- East India. Asian Pac J Cancer Prev, 14, 785-90   과학기술학회마을   DOI
18 Cubie HA, Cuschieri K (2013). Understanding HPV tests and their appropriate applications. Cytopathology, 24, 289-308
19 Datta P, Bhatla N, Dar L, et al (2010). Prevalence of human papillomavirus infection among young women in North India. Cancer Epidemiol, 34, 157-61   DOI   ScienceOn
20 Ferlay J, Shin HR, Bray F, et al (2010). Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer, 127, 2893-917.   DOI   ScienceOn
21 Ferreccio C, Barriga MI, Lagos M, et al (2012). Screening trial of human papillomavirus for early detection of cervical cancer in Santiago. Int J Cancer, 132, 916-23
22 Arbyn M, Roelens J, Simoens C, et al (2013). Human papillomavirus testing versus repeat cytology for triage of minor cytological cervical lesions. Cochrane Database Syst Rev, 3, 8054.
23 Gillio-Tos A, De Marco L, Carozzi F M, et al (2013). New Technologies for Cervical Cancer of the Hybrid Capture 2 Test: Data from the (NTCC) Study. J Clin Microbiol, 51, 2901-7   DOI
24 Giorgi-Rossi P, Franceschi S, Ronco G (2012). HPV prevalence and accuracy of HPV testing to detect high-grade cervical intraepithelial neoplasia. Int J Cancer, 130, 1387-94   DOI
25 Goldie SJ, Gaffikin L, Goldhaber-Fiebert JD, et al (2005). Cost effectiveness of cervical cancer screening in five developing countries. N Engl J Med, 353, 2158-68   DOI   ScienceOn
26 Basu P, Mittal S, Bhaumik S, et al (2013). Prevalence of high-risk human papillomavirus and cervical intraepithelial neoplasias in a previously unscreened population-a pooled analysis from three studies. Int J Cancer, 132, 1693-9   DOI
27 Bhatla N, Dar L, Patro AR, et al (2009). Can human papillomavirus DNA testing of self-collected vaginal samples compare with physician-collected cervical samples and cytology for cervical cancer screening in developing countries? Cancer Epidemiol, 33, 446-50   DOI
28 Bhatla N, Puri K, Joseph E, et al (2013). Association of Chlamydia trachomatis infection with human papillomavirus (HPV) & cervical intraepithelial neoplasia-a pilot study. Indian J Med Res, 137, 533-9
29 Bhatla N, Puri K, Kriplani A, et al (2012). Adjunctive testing for cervical cancer screening in low resource settings. Aust N Z J Obstet Gynaecol, 52, 133-9   DOI