• Title/Summary/Keyword: B-H 특성

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Chayacterization of Bacillus polyfermenticus SCD as a Probiotic. (Bacillus polyfermenticus SCD의 생균제로서의 특성)

  • 전경동;김혜진;이광호;백현동;강재선
    • Microbiology and Biotechnology Letters
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    • v.30 no.4
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    • pp.359-366
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    • 2002
  • Bacillus polyfermenticus SCD which is commonly called as Bisroot strain is being used for functional foods through the treatment of long-term intestinal disorders, since the live strains in the form of active endospores can successfully reach the target intestine in humans. The cells of B. polyfermenticus SCD were treated for 4h in artificial gastric juice (pH 2.0,3.0) and bile acid. Final viability of the strain in artificial gastric Juice (pH 2.0, 3.0) is reached to 62.8% and 81.2% respectively B. polyfermenticus SCD is resistant to antibiotics such as streptomycin, rifampicin, nystatin and ampicilin. B. polyfermenticus SCD is well known supplies the nutrients by synthesizing vitamin $B_1$, $B_2$, C and K. B. polyfermenticus SCD produces various digestive enzymes and the enzymes enable to completely digest diets in our body. Above all, $\alpha$-amylase and pretense activities are very higher than B. subtilis KCTC 1020, about two fold and twenty five fold respectively. B. polyfermenticus SCD is very stable during long-term storage period in phosphate buffers of wide-range pH, solutions of various concentrations of sodium chloride, 5% glucose solution and water.

B-H Loop Measurement of a High Tensile Steel Plate (사각판재형 강재의 자기특성측정)

  • Kim, Young-Hak;Kim, Ki-Chan;Shin, Kwang-Ho;Kim, Hwi-Seok;Yoon, Kwan-Seob;Yang, Chang-Seob
    • Journal of the Korean Magnetics Society
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    • v.20 no.3
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    • pp.94-99
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    • 2010
  • Minor B-H loop measurement for a rectangular high tensile steel was obtained by using Labview. A ferrite cores of high permeance with primary and secondary windings on the steel plate were used to form a closed loop of magnetic flux. To compensate errors due to an extremely small gap between a pair of ferrite core, and between the ferrite core and the rectangular high tensile steel, quadratic function of least square method was used. Also a 3D FEM magnetic analysis tool was used to measure H and B of the steel. B-H loop of the high tensile steel plate can be measured up to 520 A/m of a magnetic field and 0.15 T of a magnetic flux density.

Molecular Analysis and Enzymatic Characterization of Cathepsin B from Olive Flounder (Paralichthys olivaceus) (넙치 카텝신 B의 분자생물학적 분석 및 효소학적 특성 연구)

  • Jo, Hee-Sung;Kim, Na-Young;Lee, Hyung-Ho;Chung, Joon-Ki
    • Journal of Fisheries and Marine Sciences Education
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    • v.26 no.3
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    • pp.543-552
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    • 2014
  • Papain family중 하나인 cysteine protease는 근골격계 질환 치료를 위한target molecule로 인식 되어왔으며 Cathepsin B 는 단백질 분해의 초기과정에 관여하는 cysteine proteases 중 하나이다. 본 연구는 넙치의 cathepsin B 유전자의 발현 양상과 넙치 cathepsin B(PoCtB)의 클로닝, 발현 및 효소특성을 분석하였다. cDNA Library Screening을 이용하여 넙치의 cDNA를 클로닝하였다. 넙치의 동정된 cathepsin B 유전자는 993bp의 open reading frame과 330개의 아미노산으로 이루어져있다. Cathepsin B의 propeptide region 내에 GNFD motif와 occluding loop 가 존재함으로써 이것이 명백하게 cathepsin B group이라는 것을 보여주며, 계통 유전학적 분석 결과 다른 종의 cathepsin B에 비해 초창기에 분화되어 나온 것으로 사료된다. mature enzyme인 maPoCtB은 fusion protein인 glutathione S-transferase를 포함하는 pGEX-4T-1 vector에 삽입하여 E.coli 균주인 $DH5{\alpha}$ 내에 발현시켰다. 재조합 단백질인 PoCtB을 과발현 시킨 결과 53kDa의 분자량을 가진다. 넙치 cathepsin B 활성은 Z-Arg-Arg-AMC와 같은 fluorogenic 펩타이드 기질을 이용하여 측정되었고 적정 pH는 pH.7.5 이다.

Comparison of in-situ $MgB_2$ Superconducting Properties Under Different Annealing Environment (열처리조건 변화에 따른 in-situ $MgB_2$ 초전도 특성 비교)

  • Chung, K.C.;Sinha, B. B.;Chang, S.H.;Kim, J.H.;Dou, S. X.
    • Progress in Superconductivity
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    • v.14 no.2
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    • pp.116-121
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    • 2012
  • Effect of mixed gas and additional Mg powder in an annealing process of the $MgB_2$ is investigated. Four different type of samples were prepared, each in different annealing environment of Ar, $Ar+4%H_2$, Ar with Mg powder and $Ar+4%H_2$ with Mg powder. Different annealing environment did not affect the electron-phonon interaction which is reflected from the same superconducting transition of 36.6 K for all samples. The reducing effect of hydrogen is clearly depicted from the presence of excess Mg in sample synthesized in $Ar+4%H_2$ gas implying the reduced rate of reaction between Mg and B. This has manifested itself in terms of slightly increased high-field critical current density of the sample. In contrast, the sample synthesized in $Ar+4%H_2$ with Mg powder, has shown overall enhancement in the superconducting properties as presented by higher diamagnetic saturation and critical current density.

Fibrinolytic Activity and Characterization of Bacillus licheniformis HK-12 Isolated from Chungkook-Jang (청국장에서 분리한 세균인 Bacillus licheniformis HK-12의 혈전용해활성 및 특징)

  • Sohn, Byung-Hee;Song, Yu-Jin;Oh, Kye-Heon
    • KSBB Journal
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    • v.23 no.3
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    • pp.251-256
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    • 2008
  • The aim of this work was to investigate the fibrinolytic activity and characterization of Bacillus licheniformis HK-12, which produces the fibrinolytic enzyme excreted from naturally fermented Chungkook-Jang. Initially, the physiological and biochemical characteristics of strain HK-12 was examined. Both physiological analysis using BIOLOG system and phylogenetic analysis using 16S rRNA sequencing were performed to identify the strain, and the strain could be assigned to Bacillus licheniformis, designated as B. lichenformis HK-12, and registered in GenBank as [EU288193]. Phylogenetic analysis of B. licheniformis HK-12 was plotted based on 16S rRNA sequence comparisons. During the incubation period of B. licheniformis HK-12, the changes of bacterial growth, fibrinolytic activity, and pH were monitored. As the results, after 36 hours of incubation, the maximum fibinolytic activity was about 2.25 times than that of plasmin used as standard. Optimal conditions on the growth of B. licheniformis HK-12 associated with the fibrinolytic activity was initial pH 7.0 and 40$^{\circ}C$, respectively.

2 GHz Down Conversion MMIC Mixer using SiGe HBT Foundry (SiGe HBT 공정을 이용한 2 GHz Down Conversion MMIC Mixer 개발)

  • S.-M. Heo;J.-H. Joo;S.-Y. Ryu;J.-S. Choi;Y.-H. Nho;B.-S. Kim
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.13 no.8
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    • pp.764-768
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    • 2002
  • In this paper, a double balanced gilbert cell MMIC mixer was realized in Tachyonics SiGe HBT technology. The fabricated mixer has 17 dB conversion gain, 9.8 dB noise figure, -4.2 dBm output 1 dB compression point, -27 dBc RF to IF isolation, and the good input, output matching characteristics. It draws 10 mA from a 3 V supply. The simulation and the measured results are closer to each other, which confirms accuracy of the model library and reliability of the process.

The Mechanical Properties and Hand of Cotton Fabrics with the Variation of Weft Density (면직물의 위사밀도 변화에 따른 역학특성 및 태)

  • Bae Jin-Hwa;Park Jung-Whan;An Seung-Kook
    • Science of Emotion and Sensibility
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    • v.8 no.4
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    • pp.345-354
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    • 2005
  • In this study, the mechanical properties and hand characteristics have been analyze4 according to fabric structural parameters such as the weft density and weave stricture of cotton fabric. KES-FB system was used to measure hand characteristics and mechanical properties of fabric. The weft density made an effect on bending and shear properties but not on tensile , compression, and surface properties. In case of wearing property, B/w, 2HG/G, 2HB/B, 2HB/W, $\sqrt[3]{B/W}$, $\sqrt{2HB/W}$, W/T, WC/W were affected tv the weft density. The crimp was highly correlated with the tightness, hand, wearing an4 mechanical properties, specially tensile linearity, bending, shear, and compression properties. The weft crimp influenced the bending, shear, compression resilience, surface roughness, hand, and wearing properties. The tightness has an effect on tile bending, shear, compression, surface friction, hand, and wearing properties.

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An Analysis of Electrical and Mechanical Properties on Forest Fire of Polymer Insulator for T/L (송전용 플리머애자의 산불 영향에 따른 전기적 기계적 특성 분석)

  • Choi, I.H.;Choi, J.H.;Lee, D.I.;Min, B.U.;Sin, T.W.;Bang, H.K.
    • Proceedings of the KIEE Conference
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    • 2005.07c
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    • pp.2244-2246
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    • 2005
  • 우리나라 송전선로는 대부분 산지에 위치하고 있기 때문에 산불과 같은 화재가 발생 시 폴리머애자에 대한 신뢰성에 중요한 문제점을 발생시킬 수 있다. 따라서 산불의 가장자리 온도인 $600^{\circ}C$ 부근에 노출된 폴리머애자의 재질에 대한 전기적, 기계적 특성시험과 하우징 재질 특성 분석을 실시하였다. 그 결과 애자의 전기적, 기계적 시험은 기준치 이상으로 합격하였으며, 하우징 표면은 무기물이 노출되어 다공성의 특성을 보였고, 내아크시험 결과는 하부 갓의 아크소멸 시간은 A시료는 260초, 290초였고, B시료는 335초, 336초였다. 또한, 열분석 결과 $220^{\circ}C$ 근처에서 ATH의 분해가 일어나 결정수가 방출되어 실리콘 고무의 열화가 나타났다.

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