• Title/Summary/Keyword: B chain

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Immunoadjuvant Activity of Korean Mistletoe Lectin B-chain (한국산 겨우살이 Lectin B-chain의 면역증강 효과)

  • Her, Sun-Mi;An, Hyo-Sun;Kim, Kyu-Dae;Kim, Young-Hoon;Kim, In-Bo;Yoon, Taek-Joon;Kim, Jong-Bae
    • Korean Journal of Pharmacognosy
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    • v.42 no.3
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    • pp.246-252
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    • 2011
  • Korean mistletoe Lectin (KML-C) is composed of A and B sub-chain. B chain binds to carbohydrates on cell surface and A chain hinders translation and induces an apoptosis as a RIP (ribosome inactivating protein). KML-C has very strong biological activities, it has seriously limits to use as a cancer therapy or adjuvant because of its toxicity to normal cells. This study is therefore conducted to see if B chain of KML-C might have immunological activity, especially adjuvant activities with less toxicity. We isolated B chain from KML-C using the lactose affinity chromatography, and examined their immunoadjuvant activity. The isolated B-chain did not show any cytotoxicity against tumor cell, RAW264.7, and P388D1 while KML-C had a very strong toxicity. This non-toxic effect was observed also by in-vivo study. Both humoral and cellular immunities were observed ; the antibody titer was increased when the mice were immunized with B-chain used as adjuvant like Freund's adjuvant, indicating that B chain of mistletoe lectin alone might be used for adjuvant; it also increased DTH in cellular immunity. These results suggest that B-chain of KML-C might be used for adjuvant used for the production of antibody or vaccine with less toxicity.

Periplasmic Expression of a Recombinant Antibody (MabB9) in Escherichia coli

  • Chang, Hae-Choon;Kwak, Ju-Won
    • Journal of Microbiology and Biotechnology
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    • v.7 no.5
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    • pp.299-304
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    • 1997
  • Expression in the periplasm of Escherichia coli of cloned heavy and light chain cDNAs for Fab fragment of a murine monoclonal antibody MabB9 (${\gamma}2b$, K), specific for human plasma apolipoprotein B-100 of LDL, was studied. For the purpose, a vector for two-cistronic expression of the heavy chain cDNA, at the 5' terminus, and light chain cDNA, at the 3' terminus, was constructed using the signal sequences, pelB (for heavy chain) and ompA (for light chain) in a pET vector system. The constructed vector was transformed into E. coli BL21(DE3). The expressed heavy chain (25 kDa) and light chain (23 kDa) of the antibody molecule were detected in total cell extracts as well as in the periplasmic extracts of E. coli.

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A Study on Knowledge-Diffusing Mechanisms of e-Marketplace to Promote Performances of B2B Transactions (기업간 전자상거래 성과 제고를 위한 가상시장의 지식확산체계에 관한 연구)

  • Jeong, Jongsik
    • Knowledge Management Research
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    • v.3 no.2
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    • pp.17-30
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    • 2002
  • The emergence of business-to-business e-marketplace spanning both vertical and horizontal markets has relandscaped the competitive playing field in nearly every industry over the past few years by aggregating scale and spends, increasing market and value chain transparency, automating transactions along the value chain. The traits uncovered in business-to-business e-marketplaces are the taking initiative of leading companies in e-marketplace, value chain composing of promoting commerces etc, wide alliance network, and B2B in relation to B2C etc.

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Relationship between Molecular Structure of Rice Amylopectin and Texture of Cooked Rice (쌀의 아밀로펙틴 분자구조와 밥의 텍스쳐)

  • Kang, Kil-Jin;Kim, Kwan;Kim, Sung-Kon
    • Korean Journal of Food Science and Technology
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    • v.27 no.1
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    • pp.105-111
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    • 1995
  • The relationship betwwen the molecular structure of amylopectin and the texture of cooked rice was investigated using Korean rice [3 varieties of Japonica type and 3 varieties of Tongil type(Japonica-Indica breeding type)]. The molecular structure of rice amylopectin was polymodal and distributed A chain of $\overline{DP}$ 12.4, short B chain of $\overline{DP}$ 20.6, B chain of $\overline{DP}$ 26.3, long B chain of $\overline{DP}$ 45 and super long chain of above $\overline{DP}$ 55. The super long chain of amylopectin was composed of long linear chain with poorly branched chain. Also, the super long chain of amylopectin showed positive correlated with average chain length, inherent viscosity and ${\beta}-amyloysis$ limit$({\%})$, but negative correlated with ${\lambda}max$ of iodine reaction of amylopectin. The structural properties of amylopectin in Japonica type were different from those of amylopectin in Tongil type. In relationship between molecular structure of amylopectin and texture of cooked rice, the average chain length, inherent viscosity, ${\beta}-amyloysis$ limit and super long chain of amylopectin was showed a positive correlation with hardness, but a negative correlation with adhesiveness of cooked rice. The long chain of rice amylopectin is the less, the eating quality of cooled rice was the better. These results suggest that the molecular structure of rice amylopectin could be responsible for the texture of cooked rice.

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Design of Baseband Analog Chain with Optimum Allocation of Gain and Filter Rejection for WLAN Applications

  • Cha, Min-Yeon;Kwon, Ick-Jin
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.11 no.4
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    • pp.309-317
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    • 2011
  • This paper describes a baseband analog (BBA) chain for wireless local area network (WLAN) applications. For the given specifications of the receiver BBA chain, the optimum allocation of the gain and filter rejection of each block in a BBA chain is achieved to maximize the SFDR. The fully integrated BBA chain is fabricated in 0.13 ${\mu}m$ CMOS technology. An input-referred third-order intercept point (IIP3) of 22.9 dBm at a gain of 0.5 dB and an input-referred noise voltage (IRN) of 32.2 nV/${\surd}$Hz at a gain of 63.3 dB are obtained. By optimizing the allocation of the gain and filter rejection using the proposed design methodology, an excellent SFDR performance of 63.9 dB is achieved with a power consumption of 12 mW.

Solution Structure of an Active Mini-Proinsulin, M2PI: Inter-chain Flexibility is Crucial for Insulin Activity

  • Cho, Yoon-Sang;Chang, Seung-Gu;Choi, Ki-Doo;Shin, Hang-Cheol;Ahn, Byung-Yoon;Kim, Key-Sun
    • BMB Reports
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    • v.33 no.2
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    • pp.120-125
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    • 2000
  • M2PI is an active single chain mini-proinsulin with a 9-residue linker containing the turn-forming sequence 'YPGDV' between the B- and A-chains, but which retains about 50% of native insulin receptor binding activity. The refolding efficiency of M2PI is higher than proinsulin by 20-40% at alkaline pH, and native insulin is generated by the enzymatic conversion of M2PI. The solution structure of M2PI was determined by NMR spectroscopy. The global structure of M2PI is similar to that of native insulin, but the flexible linker between the B- and A-chains perturbed the N-terminal A-chain and C-terminal B-chain. The helix in the N-terminal A-chain is partly perturbed and the ${\beta}$-turn in the B-chain is disrupted in M2PI. However, the linker between the two chains was completely disordered indicating that the designed turn was not formed under the experimental conditions (20% acetic acid). Considering the fact that an insulin analogue, directly cross-linked between the C-terminus of the B-chain and the N-terminus of the A-chain, has negligible binding activity, a flexible linker between the two chains is sufficient to keep binding activity of M2PI, but the perturbed secondary structures are detrimental to receptor binding.

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Internet Usage for the Implementation of Quick Response as Supply Chain Management across Business-to-Business Electronic Commerce in Textile and Apparel Industry (섬유.의류산업의 B-to-B EC에서 SCM으로 QR 수행을 위한 인터넷 활용)

  • 오현남
    • The Research Journal of the Costume Culture
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    • v.9 no.1
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    • pp.100-110
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    • 2001
  • The purpose of this study is to identify Internet usage for the implementation of Quick Response Supply Chain Management across Business-to-Business Electronic Commerce in textile and apparel industry. This paper involves theoretical studies, which developed 3 steps to analyze the relationship of B-to-B EC, SCM, and QR, and provides broader awareness of new trend in the textile and apparel industry. SCM as one of B-to-B EC solutions introduced QR into the textile and apparel industry in 1985, and B-to-B EC is regarded as a means for achievement of QR with the widespread adoption of Internet technologies by businesses over the last four years. Finally, the Internet enables textile and apparel firms to access international networks of suppliers, distributors, and customers, so Internet-based B-to-B EC, SCM, and QR with Internet/EDI and XML/EDI are expected to become a central part in propelling fashion business into new directions.

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Fermentation and Purification of LacZ-Fused Single Chain Insulin Precursor for($B^{30}$-Homoserine) Human Insulin

  • SeungYup Lee;Jeo
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.1 no.1
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    • pp.9-12
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    • 1996
  • In order to produce the single chain precursor of a novel human insulin analogue, (B30-Homoserine) insulin, the fermentative behaviors of Escherichia coli JM103 were studied, which harbors pKBA plasmid carrying a hybrid gene in which the gene for a single chain precursor was fused with lacZ gene under tac promoter. The maximal induction of gene expression was achieved when more than 0.05 mM of isopropyl-$\beta$-D-thiogalactopyranoside(IPTG) was supplemented to fermentation medium after 4 h cultivation of E. coli, and followed by longer than 2-h fermentation. The hybrid protein of the single chain insulin precursor was isolated from cytoplasmic inclusion bodies by dissolving in 8M urea solution, and purified through DEAE-Sephacel and Sephadex G-200 column chromatographies with a recovery of 35%. The finally purified hybrid protein showed a single band on sodium dodecyl sulfate-polyacrylamide gel.

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Immunogenecity of Low Molecular Weight Immunogens in Laying Hens (닭에서 저급 분자량 항원의 면역원성)

  • Lee, Kyong-Ae
    • Korean Journal of Human Ecology
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    • v.5 no.1
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    • pp.75-80
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    • 1996
  • The immunogenecity of low molecular weight ($MW{\le}30,000$) immunogens in laying hens was investigated. Immunogens were insulin derivatives and ${\beta}$-lactoglobulin(${\beta}-Lg$). Insulin derivatives were reduced- carboxymethylated(RCM) insulin, and RCM-A and RCM-B chain of insulin. The yolk antibodies against RCM-A chain of insulin appeared after the first immunization. The yolk antibodies against RCM-B chain of insulin were elicited 5 weeks after the third booster injection. Although the anti-RCM-B chain yolk antibodies recognized native insulin, the anti-RCM-A chain yolk antibodies didn't native insulin. The anti-RCM insulin yolk antibodies were induced after the second booster injection and showed cross-reactivities with native insulin. On the other hand, ${\beta}-Lg$ showed stronger immunogenecity than insulin derivatives. The $anti-{\beta}-Lg$ yolk antibodies were produced after the second booster injection and the peak titer was reached 3 weeks after the third booster injection.

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Evidence for VH Gene Replacement in Human Fetal B Cells

  • Lee, Jisoo;Cho, Young Joo;Lipsky, Peter E.
    • IMMUNE NETWORK
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    • v.2 no.2
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    • pp.79-85
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    • 2002
  • Background: In contrast to evidences of Ig H chain receptor editing in transformed cell lines and transgenic mouse models, there has been no direct evidence that this phenomenon occurs in human developing B cells. Methods: $V_HDJ_H$ rearrangements were obtained from genomic DNA of individual $IgM^-$ B cells from liver and $IgM^+B$ cells from bone marrow of 18 wk of gestation human fetus by PCR amplification and direct sequencing. Results: We found three examples of H chain receptor editing from $IgM^+$ and $IgM^-human$ fetal B cells. Two types of $V_H$ replacements were identified. The first involved $V_H$ hybrid formation, in which part of a $V_H$ gene from the initial VDJ rearrangement is replaced by part of an upstream $V_H$ gene at the site of cryptic RSS. The second involved a gene conversion like replacement of CDR2, in which another $V_H$ gene donated a portion of its CDR2 sequence to the initial VDJ rearrangement. Conclusion: These data provide evidence of receptor editing at the H chain loci in developing human B cells, and also the first evidence of a gene conversion event in human Ig genes.