• BMB Reports
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• v.50 no.7
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• pp.384-389
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• 2017

The Nogo-B receptor (NgBR) is necessary for not only Nogo-B-mediated angiogenesis but also vascular endothelial growth factor (VEGF) -induced angiogenesis. However, the molecular mechanisms underlying the regulatory role of the VEGF-NgBR axis in angiogenesis are not fully understood. Here, we report that miR-26a serves as a critical regulator of VEGF-mediated angiogenesis through directly targeting NgBR in endothelial cells (ECs). Stimulation of ECs by VEGF increased the expression of NgBR and decreased the expression of miR-26a. In addition, miR-26a decreased the VEGF-induced migration and proliferation of ECs. Moreover, miR-26a overexpression in ECs decreased the VEGF-induced phosphorylation of the endothelial nitric oxide synthase (eNOS) and the production of nitric oxide, which is important for angiogenesis. Overall, these data suggest that miR-26a plays a key role in VEGF-mediated angiogenesis through the modulation of eNOS activity, which is mediated by its ability to regulate NgBR expression by directly targeting the NgBR 3'-UTR.

• Bulletin of the Korean Chemical Society
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• v.14 no.2
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• pp.266-271
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• 1993

The reaction of $(CO_5$)M=C(OMe)Tol (M=Cr, Mo, W and $Tol=p-C_6H_4Me)$ and $BBr_3$ followed by treatment with tetramethylethylenediamine (TMEDA) yields a mixture of two diastereomers, trans, $cis-Br(CO)_2(tmeda)M{\equiv}$CTol [M=Cr(1a), Mo(2a), W(3a)] and cis, $trans-Br(CO)_2(tmeda)M{\equiv}$CTol [M=Cr(1b), Mo(2b), W(3b)], respectively. These compounds have been isolated as crystalline solids and characterized by spectroscopic (infrared, mass, $^1H$ and $^{13}C-NMR)$ data. The trans, cis-Br(CO)2(tmeda)Cr${\equiv}$CTol (1a), has been examine via a single crystal X-ray diffraction study : $BrCrO_2N_2C_{16}H_{23}$, Mr=407.27, triclinic, $P{\bar{1}},\;a=12.792(2),\;b=13.400(5),\;c= 11.645(4)\;{\AA},\;{\alpha}=101.26(2)^{\circ},\;{\beta}=103.04(2)^{\circ},\;{\gamma}=91.88(2)^{\circ},\;{\nu}=1907(1){\AA}^3,\;Z=2,\;{\rho}(calcd)=1.418\;gcm^{-3},\;{\lambda}(MoK{\alpha})=0.71069\;{\AA},\;{\mu}=26.25 cm^{-1},\;F(000)=831.97,\;T=295K,\;R=0.0977$ for 1332 significant reflections $[F_0>5{\sigma}(F_0)]$. There are two essentially equivalent molecules in the crystallographic asymmetric unit. Each molecule is octahedral with the bromide ligand trans to the alkylidyne carbon, the two cis-carbonyl ligands, and the bidentate TMEDA ligand.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.26 no.9
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• pp.1241-1248
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• 2002

Performance extension of the absorption refrigerator with LiBr solution is often faced to operate very close to the crystallization limit. Especially in the development of an air-cooled cycle, the crystallization of working solution in the system is a very difficult problem to overcome. This paper describes the cycle of hot water driven absorption system using a new working absorption solution instead of LiBr solution to improve the efficiency. In this study, we found out the characteristics of new working absorption solution through the cycle simulation and compared LiBr solution to evaluate. The effect of cooling water temperature, weak solution flow rate, hot water temperature and hot water flow rate were also examined. The COP is increased 22% higher in the case of LiBr＋Li1＋LiC1＋LiNO$_3$＋$H_2O$, 2% LiBr＋HO(CH$_2$)$_3$OH＋$H_2O$ than that of LiBr solution for the same operation condition.

• Korean Journal of Crystallography
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• v.15 no.1
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• pp.5-8
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• 2004

The hydrothermal reaction of copper(I) bromide (CuBr) and pyrazine ($C_4H_4N_2$, pyz) gave a 2-D corrdination polymer [CuBr(pyz)] (1). X-ray structure determination revealed that polymer 1 has a 2-D network based on rectangular grids, each of which has the dimensions of 4.0${\times}$5.7 ${\AA}$. Polymer 1 has channels along the b-axis.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.33 no.3
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• pp.110-115
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• 2023

During the Hg₂Br₂ physical vapor transport process, with increasing the partial pressure of component B, P_B from 40 Torr to 200 Torr, a unicellular convective flow structures move from the crystal growth region to the center region in the vapor phase. The boundary layer flow is dominant for P_B = 40 Torr, and the core region flow is dominant for P_B = 200 Torr. The flow in the vapor phase shows a three-dimensional convective flow structure with a single cell (unicellular) for P_B = 40 Torr and 200 Torr, exhibits an asymmetrical flow with respect to the x, y central axis under the horizontally oriented configuration with an aspect ratio (length-to-width) of 3 and linear conducting walls. The critical temperature difference between the source and crystal region is about 30 K. The total molar flux of Hg₂Br₂ increases with the temperature difference until the total molar flux reaches the critical value. At the critical total molar flux, the total molar flux abruptly decreases.

• Microbiology and Biotechnology Letters
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• v.50 no.3
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• pp.395-403
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• 2022

In this study, we identified that the fermentation of Korean indigenous probiotics and red ginseng produced ginsenoside compound K (CK) from major ginsenosides. Based on whole genome sequencing of 19 probiotics species, β-glucosidase, α-arabinofuranosidase, β-xylosidase, and α-rhamnosidase related to bioconversion of ginsenosides are identified in the genome of 19 species, 3 species, 6 species, and 8 species, respectively. Among the 19 probiotics species, Bifidobacterium longum CBT BG7 converted from ginsenoside Rb1 to CK, and both B. breve CBT BR3 and B. lactis CBT BL3 converted ginsenoside Rb1 to Rd. The final concentration and yield of ginsenoside F2 and CK were higher in the fermentation with the nondisrupted cells than with disrupted cells. The combination of both CBT BG7 and BL3, and CBT BG7 and BR3 showed higher amounts of F2 than CBT BG7 only. CBT BG7 with adding α-amylase increased the amounts of F2. In this study, we identified that the fermentation of both Korean indigenous probiotic bacteria CBT BG7, BR3 and BL3, and red gingseng is able to produce CK, a bioactive compound that promotes health benefits.

• Korean Journal of Breeding Science
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• v.50 no.4
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• pp.378-386
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• 2018

GROWTH-REGULATING FACTOR (GRF) genes encode plant-specific transcription factors and play critical roles in regulating the growth and development of lateral organs. In order to explore the agricultural potential of Brassica rapa GRF genes (BrGRFs), we constructed two BrGRF-overexpressing B. napus plants (BrGRF3-1OX and -9OX). BrGRF3-1OX and -9OX developed larger cotyledons, leaves, and seeds than the wild type. The increased organs' sizes were due to increases in cell number, but not due to cell size alterations. RT-PCR analysis revealed that BrGRFs regulated the expression of a wide range of genes that are involved in gibberellin-, auxin-, cell division-related growth processes. Taken together, our data indicate that BrGRFs act as positive regulators of B. napus growth, thus raising the possibility that they may serve as a useful genetic source for crop improvement with respect to organ size and seed production.

• Journal of Sericultural and Entomological Science
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• v.35 no.2
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• pp.129-133
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• 1993

The 44Md plasmid of Bacillus thruingiensis var. kurstaki HD-1(B. t k HD-1) was partially digested with Sau3AI and the fragments were cloned into E. coli HB101 on vector pBR322. Of 2, 950 clones with a recombinant pBR322, only one clone KC1 was determined to have the gene for crystal toxic proteins from the 44Md plasmid of B. t k HD-1 at the BamHI site of pBR322. The recombinant pBR322 was named pKC1 and its molecular size was 12kb. The KC1 produced a protein which was toxic to the silkworm and antigenically similar to the crystal toxic protein of B. t k HD-1. Also, electrophoretic mobility of the KC1 protein was apparently the same as that of the crystal toxic protein of B. t k HD-1.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.21 no.2
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• pp.294-302
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• 1997

Falling-film evaporation experiments for aqueous solution of lithium bromide (LiBr) were performed on a horizontal smooth 19.05-mm-dia copper tube. Average heat transfer coefficients were obtained with varied film Reynolds numbers, system pressures, LiBr concentrations and degrees of wall superheat. Heat transfer coefficients increase with increasing system pressure and decreasing concentration. For degrees of wall superheat, the heat transfer coefficient did not't show the distinct trend. For this experimental ranges, heat transfer coefficients showed maximum values at an optimal film Reynolds number. The results of this work were compared with pool boiling data reported previously, and it was shown that the heat transfer performance is superior to the pool boiling.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.20 no.2
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• pp.670-679
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• 1996

A numerical study for vapor absorption into LiBr-H$_{2}$O solution film flowing over horizontal circular tubes has been carried out. The momentum, energy and diffusion equations, which are parabolized by the boundary- layer approximation, are solved simultaneously for various mass-flow rates and inlet conditions. The results for the velocity, temperature and concentration fields, as well as the heat and mass flux at the free surface are presented. The effects of inlet conditions, i.e., flow rate, temperature and concentration, on the absorption process are also examined and discussed.