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http://dx.doi.org/10.9787/KJBS.2018.50.4.378

Effects of Overexpression of Brassica rapa GROWTH-REGULATING FACTOR Genes on B. napus Organ Size  

Hong, Joon Ki (Agricultural Biotechnology Department, National Institute of Agricultural Sciences, Rural Development Administration)
Suh, Eun Jung (Agricultural Biotechnology Department, National Institute of Agricultural Sciences, Rural Development Administration)
Lee, Seung-Bum (Agricultural Biotechnology Department, National Institute of Agricultural Sciences, Rural Development Administration)
Yoon, Hye-Jin (Agricultural Biotechnology Department, National Institute of Agricultural Sciences, Rural Development Administration)
Lee, Yeon-Hee (Agricultural Biotechnology Department, National Institute of Agricultural Sciences, Rural Development Administration)
Publication Information
Korean Journal of Breeding Science / v.50, no.4, 2018 , pp. 378-386 More about this Journal
Abstract
GROWTH-REGULATING FACTOR (GRF) genes encode plant-specific transcription factors and play critical roles in regulating the growth and development of lateral organs. In order to explore the agricultural potential of Brassica rapa GRF genes (BrGRFs), we constructed two BrGRF-overexpressing B. napus plants (BrGRF3-1OX and -9OX). BrGRF3-1OX and -9OX developed larger cotyledons, leaves, and seeds than the wild type. The increased organs' sizes were due to increases in cell number, but not due to cell size alterations. RT-PCR analysis revealed that BrGRFs regulated the expression of a wide range of genes that are involved in gibberellin-, auxin-, cell division-related growth processes. Taken together, our data indicate that BrGRFs act as positive regulators of B. napus growth, thus raising the possibility that they may serve as a useful genetic source for crop improvement with respect to organ size and seed production.
Keywords
Cell division; Cell number; GROWTH-REGULATING FACTOR; Organ size; Transcription factor;
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