• 한국미생물·생명공학회지
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• 제20권1호
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• pp.79-84
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• 1992

Rhodopseudomonas sphaeoides B6 세포를 agar gel 중에 고정했을 때, 수소생산을 위한 최적 agar 농도는 2(w/v)였다. B6의 2% agar gel 고정화균체(300ml gel: 2.85 mg dry cells/ml)와 비고정세포(1l culture: 0.87 mg dry cells/ml)에 있어서 초기의 최고 수소 생산활성은 각각 47.5 및 48.0ml/hr/culture로 거의 같았다. 그러나, 수소생산이 거의 정지된 배양후기에는 lactate의 제한공급( 10 mmole)에 의한 비고정세포의 활성회복은 50% 이하로 감소되었지만, 고정화세포의 활성은 거의 초기상태로 회복되었다. B6의 고정화균체를 이용하여 12시간 주기의 광조사조건으로, 매 1l의 수소생산시 마다 그에 소비된 만큼의 기질에 상당하는 9.3mmole의 DL-lactate와 1.86mmole의 L-glutamate 함유 기본배지를 주기적으로 공급한 결과, 228시간의 배양기간 동안 명암의 반복조건에도 불구하고 평균 510 ml/day/300m/ gel(2.9mg dry cells/ml)의 속도로 수소생산이 지속되었다. 이 결과는 광합성세균에 의한 수소생산에 있어서, 보다 효율적 기질공급과 가변적인 태양광 이용 조건에 대응되는 자동배양 시스템의 구성 가능성을 시사한다.

• Asian-Australasian Journal of Animal Sciences
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• 제28권11호
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• pp.1629-1640
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• 2015

This study was performed to compare carcass traits, sensory characteristics, physiochemical composition, and contents of nucleotides, collagen, and free amino acids among quality grades (QG) and to understand the association between QG and above parameters in loin and rump of Korean cattle steer. Loin and rump samples were obtained from 48 Korean cattle steers with each of four QG (QG 1++, 1+, 1, and 2; average 32 months of age). Carcass weight and marbling score (MS) were highest in QG 1++, whereas texture score measured by a meat grader was highest in QG 2. A correlation analysis revealed that MS (r = 0.98; p<0.01) and fat content (r = 0.73; p<0.01) had strong positive correlations with QG and that texture had a strong negative correlation (r = -0.78) with QG. Fat content in loin was highest but protein and moisture contents were lowest in QG 1++. Our results confirmed that a major determinant of QG is the MS; thus, intramuscular fat content. The International Commission on Illumination $L^*$, $a^*$, and $b^*$ values in loin were highest in QG 1++. Numeric values of shear force in loin were lowest in QG 1++, whereas those of tenderness, juiciness, and overall acceptability tended to be highest in QG 1++ without statistical significance. QG was strongly correlated with juiciness (r = 0.81; p<0.01) and overall acceptability (r = 0.87; p<0.001). All sensory characteristics were higher (p<0.05) in loin than those in rump. Adenosine-5'-monophosphate (AMP) and inosine-5'-monophosphate (IMP) contents in both loin and rump did not differ among QGs. No nucleotide (AMP, IMP, inosine, hypoxanthine) was correlated with any of the sensory traits. Total, soluble, and insoluble collagen contents in loin were higher in QG 1++ than those in QG 1. All three collagens had lower content in loin than that in rump. All three collagens were positively correlated with tenderness, juiciness, and overall acceptability. Glutamic acid content did not significantly differ among the four QGs in either loin or rump. In conclusion, it is confirmed that QG is associated with sensory traits but nucleotide contents in beef may not be a major factor determining meat palatability in the present study.

• Journal of Plant Biotechnology
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• 제1권1호
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• pp.1-7
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• 1999

Large-scale cultures of plant cell, tissue, and organ have been achieved by using BTBB. When different sized BTBBs (5 L, 20 L, 100 L, 300 L, and 500 L) were tested for the culture of yew cells (Taxus cuspidata Sieb. et Zucc.), cell growth increment reached to 94.5% in SCV after 24 days of culture with 30% of inoculation cell density. However, there were some variations in the production of taxol and its derivatives among the BTBBs of different size. Approximate 4 ㎎/l of taxol and 84 ㎎/l of total taxanes were obtained by using a 500L BTBB after 6 weeks of culture. With a 20L BTBB, about 20,000 cuttings of virus-free potatoes (cv. Dejima) could be obtained by inoculating 128 explants and maintaining 8 weeks under 16 hr light illumination. The frequency of ex vitro rooting of the cuttings revealed as more than 99% under 30% shade. By incorporating two-stage culture process consisting of multiple bulblet formation in solid medium and bulblet development in liquid medium, mass propagation of lily through bioreactor seemed to be possible. In the case of 'Marcopolo', the growth of mini-bulblets in BTBB was nearly 10 folds faster than that of the solid medium. Time course study revealed that maximum MAR yield of ginseng (Panax ginseng C. A. Meyer) in a 5 L and 20 L BTBB after 8 weeks of culture was 500 g and 2.2 ㎏, respectively. By cutting the MAR once and/or twice during the culture, the yield of root biomass could be increased more than 50% in fresh weight at the time of harvest. With initial inoculum of 500 g of sliced MAR in a 500 L BTBB, 74.8 ㎏ of adventitious root mass was obtained after 8 weeks of culture. The average content of total ginseng saponin obtained from small-scale and/or pilotscale BTBBs was approximately 1% per gram dry weight. Based on our results, we suggest that large-scale cultures of plant cell, tissue, and organ using BTBB system should be quite a feasible approach when compared with conventional method of tissue culture.

• 대한치과보철학회지
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• 제44권3호
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• pp.284-294
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• 2006

Statement of problem. Proliferation of Candida albicans is primarily within the plaque on the fitting surface of the denture rather than on the inflamed mucosa. Consequently, the treatment of the denture is equally important as treatment of the tissue. Cleansing and disinfection should be efficiently carried-out as the organisms can penetrate into the voids of the acrylic resin and grow in them, from which they can continue to infect and reinfect bearing tissues. Purpose. The purpose of this study was to evaluate the applicability of photocatalytic reaction to eliminate Candida albicans from acrylic resin denture base, and to investigate the anti-fungal effect with various UVA illumination time. Materials and Methods. The specimens were cured by the conventional method following the manufacturer's instruction using thermal polymerized denture base resin (Vertex RS: Dentimex, Netherlands). $TiO_2$ photocatalyst sol(LT), which is able to be coated at normal temperature, was made from the Ti-alkoxide progenitor. The XRD patterns, TEM images and nitrogen absorption ability of the $TiO_2$ photocatalyst sol(LT) were compared with the commercial $TiO_2$ photocatalyst P-25. The experimental specimens were coated with the mixture of the $TiO_2$ photocatalyst sol(LT) and binder material (silane) using dip-coater, and uncoated resin plates were used as the control group. Crystallinity of $TiO_2$ of the specimen was tested by the XRD. Size, shape and chemical compositions were also analyzed using the FE-SEM/ EDS. The angle and methylene blue degradation efsciency were measured for evaluating the photocatalytic activity of the $TiO_2$ film. Finally, the antifungal activity of the specimen was tested. Candida albicans KCTC 7629(1 ml, initial concentration $10^5$ cells/ ml) were applied to the experiment and control group specimens and subsequently two UVA light source with 10W, 353 nm peak emission were illuminated to the specimens from 15cm above. The extracted $2{\mu}l$ of sample was plated on nutrient agar plate ($Bacto^{TM}$ Brain Heart Infusion; BD, USA) with 10 minute intervals for 120 minute, respectively. It was incubated for 24 hours at $37^{\circ}C$ and the colony forming units (CFUs) were then counted. Results. Compared the characteristics of LT photocatalyst with commercial P-25 photocatalyst, LT were shown higher activity than P-25. The LT coated experimental specimen surface had anatase crystal form, less than 20 nm of particle size and wide specific surface area. To evaluate the photocatalytic activity of specimens, methylene blue degradation reaction were used and about 5% of degradation rate were measured after 2 hours. The average contact angle was less than $20^{\circ}$ indicating that the LT photocatalyst had hydrophilicity. In the antifungal activity test for Candida albicans, 0% survival rate were measured within 30 minute after irradiation of UVA light. Conclusion. From the results reported above, it is concluded that the UVA-LT photocatalytic reaction have an antifungal effect on the denture surface Candida albicans, and so that could be applicable to the clinical use as a cleaning method.

• 가정∙방문간호학회지
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• 제12권2호
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• pp.1-15
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• 2005

Purpose: This study was conducted to assess the fall risk factors. Method: The subjects were 87 persons who were older than 65 years living at 28 nursing home in Seoul and Gyunggi province. Subjects were interviewed using RAFS II for intrinsic factor and the environmental factor were assessed using a structured questionnaire from Oct. to Dec. in 2004. The data were analyzed by SPSS(ver. 12.0) programs, using descriptive statistics, $x^2$-test, and t-test. Result: The 37.9% of the subjects experienced the fall, and its average number is $1.94\pm1.75$. The Women's experience of the fall was higher than that of the men, but it was no significant difference with gender. There was no significant difference with the age and duration of living. The mean of the intrinsic risk factor was 13.38 in total score 39 points marks on the RAFS II scales. The intrinsic risk factor score of the fallen group 15.71 was significantly higher than the non-fallen group 12.10. The variables of recent fall experience(t=4.72, p=0.000) and urinary dysfunction(t=2.64, p=0.010) was significantly higher than the non-fallen group. The highest variable of the intrinsic factor was the age and the variables of drug intake, balance, chronic disease, recent fall experience, urinary dysfunction were followed in order. The mean of the environmental risk factor was 0.24 points. No significant differences were shown in environmental risk factor between the fallen and the non-fallen groups. To the fallen group, the place of entrance was the highest risky environmental factor. To see in area dimension, the floor surfaces was the highest risky environmental factor and equipment and illumination factor was the following risky elements in order. In the total score of environmental risk factors based on the ares, the fallen group was 0.26 and the non-fallen group was 0.24, but there were no significant differences between the groups. Conclusion: This results suggested that visual protection strategy, set up the safety device in the place of entrance and inner stairway, bathroom and nonskid mat in the nursing home would be contribute to the prevention of the fall for the elderly.

• 수산해양기술연구
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• 제31권3호
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• pp.213-219
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• 1995

색광에 대한 감성돔, Acanthopagrus schlegelii [Bleeker]의 행동을 조사하기 위하여, 2가지씩의 서로 다른 색광을 조합하여 수조의 양단에서 동시에 광자극을 가했을 때의 반응을 주간과 야간으로 구분 조사한 결과는 다음과 같다. 1. 수조내에서의 감성돔의 구간별 분포곡선은 중앙구간보다 양단구간에 많이 모여서 대체로 U자형이 되었으며, 주야간의 차이도 다소 있었다. 2. 양색광쪽에서의 평균 분포차는 7.18%(0.35~19.55%)였고, 주간(8.20%) 보다 야간(6.15%)에 더 적었다. 3. 감성돔이 잘 모이는 색광은 주간에는 청색, 황색, 적색, 백색의 순이며, 야간에는 청색, 적색, 황색, 백색의 순으로 나타났다. 4. 조명시간의 경과에 따른 집어율의 변화는 일정한 증감추세를 보이지 않고 계속 불안정하게 변동했다. 5. 두 가지 색광에 대한 집어율의 차는 비교적 뚜렷하지 않았으나, 주간보다 야간에 그 차가 심했다.

• 한국융합학회논문지
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• 제10권6호
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• pp.7-14
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• 2019

본 논문에서는 YCbCr 색공간을 이용한 피부색 추출에서 조명과 그림자에 의한 손실 영역을 Flood Fill 알고리즘을 이용하여 보완하고 Haar-like 특징을 이용한 Cascade Classifier 얼굴 검출 방법을 제안하였다. Haar-like 특징을 이용한 Cascade Classifier는 이미지에서 기존의 YCbCr 색공간을 이용한 피부색 추출은 단순히 임계값만 사용하기 때문에 조명, 그림자 등에 의해 잡음과 손실 영역이 발생할 수 있다. 이러한 문제를 해결하기 위해 침식, 팽창 연산을 사용하여 잡음을 제거하였고 손실 영역을 추정하기 위해 Flood Fill 알고리즘을 사용하여 손실 영역을 추정하였다. 추정한 영역에 대하여 YCbCr 색공간의 임계값을 추가로 허용하였다. 나머지 손실영역에 대하여 위에서 추정한 영역중 추가로 허용한 영역의 평균값으로 색을 채워 넣었다. 추출한 이미지에 Haar-like Cascade Classifier를 사용하여 얼굴을 검출하였다. 기존의 Haar-like Cascade Classifier의 방법보다 제안하는 방법이 정확도가 약 4% 향상되었으며 YCbCr 색공간만을 이용한 피부색 추출보다 제안하는 방법의 검출률이 약 2% 향상되었다.

• 정보처리학회논문지:소프트웨어 및 데이터공학
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• 제12권6호
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• pp.267-274
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• 2023

본 논문에서는 입력 영상에 대한 촬영 장면의 조명 색도를 추정하는 방법을 제안한다. 조명 기준영역을 이용하여 입력영상의 촬영 장면에 가장 근접한 조명 색도를 추정한다. 기존의 방법은 일정한 수의 기준조명 정보를 이용한다. 입력 영상으로부터 화소의 색도분포 정보와 기준 조명에 대한 미리 준비된 색도 집합을 대조하여 겹치는 면적이 가장 큰 기준 조명을 해당 입력 영상에 대한 장면 조명으로 간주한다. 겹치는 면적을 계산하는 과정에서 각 기준 조명에 대한 가중치를 가우시안 분포 형태로 적용하였으나, 분산 값에 대하여 명확한 기준을 제시하지 못하였다. 제안한 방법은 주어진 기준조명으로부터 독립적인 기준색도 영역을 추출하고, 입력영상의 모든 화소에 대하여 RGB 칼라좌표계의 r-g 색도 평면에서의 특징치를 계산한 다음, 독립적인 색도영역과 입력영상으로부터의 특징치를 이용하여 유사도를 평가한다. 유사도가 가장 높게 나타나는 조명을 해당 영상의 조명 색도 성분으로 추정하였다. 데이터베이스의 영상과 기준조명 색도를 이용한 성능평가에서 제안한 방법은 기존의 기본 방법에 비하여 평균 60% 정도의 개선을 보였고, 기존의 가우시안 분산 값이 0.1인 경우에 비하여 53% 내외의 개선 성능을 보였다.

• 대한원격탐사학회지
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• 제40권4호
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• pp.397-418
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• 2024

The Land Surface Temperature (LST) is a crucial parameter in identifying drought. It is essential to identify how LST can increase its accuracy, particularly in mountainous and hill areas. Increasing the LST accuracy can be achieved by applying early data processing in the correction phase, specifically in the context of topographic correction on the Lambertian model. Empirical evidence has demonstrated that this particular stage effectively enhances the process of identifying objects, especially within areas that lack direct illumination. Therefore, this research aims to examine the application of the Lambertian model in estimating LST using the Multi-Channel Method (MCM) across various physiographic regions. Lambertian model is a method that utilizes Lambertian reflectance and specifically addresses the radiance value obtained from Sun-Canopy-Sensor(SCS) and Cosine Correction measurements. Applying topographical adjustment to the LST outcome results in a notable augmentation in the dispersion of LST values. Nevertheless, the area physiography is also significant as the plains terrain tends to have an extreme LST value of ≥ 350 K. In mountainous and hilly terrains, the LST value often falls within the range of 310-325 K. The absence of topographic correction in LST results in varying values: 22 K for the plains area, 12-21 K for hilly and mountainous terrain, and 7-9 K for both plains and mountainous terrains. Furthermore, validation results indicate that employing the Lambertian model with SCS and Cosine Correction methods yields superior outcomes compared to processing without the Lambertian model, particularly in hilly and mountainous terrain. Conversely, in plain areas, the Lambertian model's application proves suboptimal. Additionally, the relationship between physiography and LST derived using the Lambertian model shows a high average R² value of 0.99. The lowest errors(K) and root mean square error values, approximately ±2 K and 0.54, respectively, were achieved using the Lambertian model with the SCS method. Based on the findings, this research concluded that the Lambertian model could increase LST values. These corrected values are often higher than the LST values obtained without the Lambertian model.

• 생물환경조절학회지
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• 제21권3호
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• pp.220-227
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• 2012

폐쇄형 묘생산 시스템에서 청색LED, 적색LED, 백색형광등을 인공광원으로 이용한 가운데 파프리카의 육묘시 생장 특성과 정식 후 생장 및 초기 수량을 분석하고자 본 연구가 수행되었다. 폐쇄형 시스템에서 파프리카 육묘용 환경조건은 광주기 16/8h, 평균 PPF $204{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, 기온 26/$20^{\circ}C$, 상대습도 70%이었다. 육묘 후 21일째에 백색형광등과 LED 하에서 생장된 파프리카 묘의 엽장, 엽폭, 엽면적 등 잎 관련지표뿐만 아니라 지상부 생체중과 건물중, 엽록소함량 등이 자연광 처리구에 비해서 크게 나타났다. 청색 LED, 적색LED 및 자연광 처리구에서의 엽면적은 대조구인 형광등 처리구와 비교할 때 각각 63%, 63%, 28%에 해당하였다. 또한 청색LED, 적색LED 및 자연광 처리구의 지상부 건물중은 각각 대조구의 64%, 50%, 22%로 나타났다. 정식 후 18일째에 엽수는 대조구에서 44매로 가장 크게 나타났다. 적색LED, 청색LED 및 자연광 처리구의 엽수는 대조구에 비해서 각각 86%, 81%, 48%로서 정식 시기와 비교할 때 엽수의 차이가 줄어들었다. 정식 후 114일째에 초장은 청색LED와 적색LED 처리구에서 상대적으로 작게 나타났다. 이러한 결과는 단색LED 하에서 육묘된 파프리카의 줄기 신장이 정식 후에 억제된 것으로 판단된다. 초기 4주 동안 수확된 파프리카는 청색LED 3.5개/plant, 적색LED 3.3개/plant, 자연광 1.0개/plant으로서 대조구 2.2개/plant에 비해서 각각 159%, 150%, 45%로 나타났다. 초기수량은 적색LED 453g/plant, 청색LED 403g/plant, 자연광 101g/plant으로서 대조구 273g/plant와 비교할 때 각각 166%, 148%, 37%로 나타났다. 한편 적색LED 처리구에서의 평균 중량은 136g으로서 다른 처리구와 비교할 때 상대적으로 큰 과실이 생산되었다. 한편 정식 후 온실에서의 재배기간이 길어짐에 따라 인공광 처리구와 자연광 처리구에서 수량 차이가 없었다. 이러한 결과를 종합하면 LED 또는 형광등을 인공광원으로 이용한 조건에서 육묘된 파프리카의 정식 후 초기 생육이 양호하였으며, 초기 수확이 자연광 처리구에 비해서 1주 정도 빠르게 이루어졌음을 알 수 있다. 따라서 LED 또는 형광등과 같은 인공광원이 파프리카 육묘에 이용될 경우 묘소질의 향상, 조기 수확 및 초기 수량의 증대가 기대된다.