• Korean Journal of Oriental Medicine
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• v.16 no.1
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• pp.135-140
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• 2010

This study was carried out to investigate the acute toxicity and safety of Ssanghwa-tang extract fermented with Ganoderma lucidum. To evaluate the acute toxity and safety, 0(control group), 1250, 2500 and 5000 mg/kg of Ssanghwa-tang and fermented Ssanghwa-tang extracts were orally administered to 20 male and 20 female ICR mice. After single administration, we observed survival rates, general toxicity, changes of body weight, and autopsy. Compared with the control group, we could not find any toxic alteration in all treated groups (1250, 2500 and 5000 mg/kg). $LD_{50}$ of Ssanghwa-tang and fermented Ssanghwa-tang extracts might be over 5000 mg/kg and it is very safe to ICR mice.

• Korean Journal of Oriental Medicine
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• v.15 no.3
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• pp.93-98
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• 2009

In this research, the acute toxicity of fermented Yukmijihwangtang extract was examined using male and female ICR mice, To evaluate the acute toxity and safety, 0(control group), 1250, 2500 and 5000 mg/kg of fermented Yukmijihwangtang extract were orally administered to male and female ICR mice. After single administration, we observed survival rates, general toxicity, changes of body weight for the 14 days and autopsy at 1 day following the administration according to the Regulation of Korean Food and Drug Administration. Compared with the control group, we could not find any toxic alteration in all treated groups (1250, 2500 and 5000 mg/kg). $LD_{50}$ of fermented Yukmijihwangtang extract might be over 5000 mg/kg and it is very safe to ICR mice.

• Archives of Plastic Surgery
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• v.34 no.4
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• pp.426-431
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• 2007

Purpose: The aim of this study is to compare the effects of bone marrow stromal cells(BSCs) and fibroblasts on wound healing activity in vivo, especially on epithelization. Methods: The fibroblasts and BSCs were harvested from patients and cultured. Ten Spague-Dawley white rats were used. A 5 mm punches were made to excise skin and subcutaneous tissue in a round fashion at six sites on the back area of each rat. Four hundred thousand cells suspended in 0.05 ml fibrinogen were applied to the created wounds. The cells in group I, II, and III were no cells, fibroblasts and BSCs. The lengths of epithelial gap at the widest wound site were compared with autopsy specimens obtained on the 6th day after cell therapy under light microscope. Statistical comparisons were performed using the Mann-Whitney U-test, and the p value < 0.05 was considered statistically significant. Results: The best epithelization was also seen in the BSC group, followed by fibroblast and no cell groups.Conclusion: These results demonstrate that BSC has superior effect on stimulating wound healing than fibroblast, which is currently used for wound healing.

• Journal of Veterinary Clinics
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• v.24 no.2
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• pp.264-268
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• 2007

Three German Shepherd dogs (5 years old, 30 kg, male; 4 years old, 23 kg, female; 4 years old, 24 kg, female) with history of acute vomiting, hemorrhagic diarrhea, anorexia and dullness were admitted to veterinary clinic in the military working dogs training corp (MWDTC). All of them showed same symptoms after ingestion the $'PREVNTIC^{(R)}'$ collar to control ecto-parasites infection in field. One dog was operated by gastrotomy and the $5{\sim}6$ pieces of the $'PREVNTIC^{(R)}'$ collar were removed. The dog was completely cured by intensive care within 2 hours after $'PREVNTIC^{(R)}'$ collar ingestion. However, two dogs were operated by gastrotomy and intensive care over 24 hours after $'PREVNTIC^{(R)}'$ collar ingestion but died. In autopsy, the $5{\sim}6$ pieces of hard materials were found in the stomach, and severe thickness, congestion hemorrhage and swelling of the stomach walls and mucous membrane were observed. In these cases, it was suggested that the first aid of treatment such as gastrotomy within two hours after $'PREVNTIC^{(R)}'$ collar ingestion as soon as possible was helpful for attenuating of toxicosis.

• Journal of Microbiology
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• v.43 no.spc1
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• pp.65-84
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• 2005

Invasive candidiasis is associated with high morbidity and mortality. Clinical diagnosis is complicated by a lack of specific clinical signs and symptoms of disease. Laboratory diagnosis is also complex because circulating antibodies to Candida species may occur in normal individuals as the result of commensal colonization of mucosal surfaces thereby reducing the usefulness of antibody detection for the diagnosis of this disease. In addition, Candida species antigens are often rapidly cleared from the circulation so that antigen detection tests often lack the desired level of sensitivity. Microbiological confirmation is difficult because blood cultures can be negative in up to 50% of autopsy-proven cases of deep-seated candidiasis or may only become positive late in the infection. Positive cultures from urine or mucosal surfaces do not necessarily indicate invasive disease although can occur during systemic infection. Furthermore, differences in the virulence and in the susceptibility of the various Candida species to antifungal drugs make identification to the species level important for clinical management. Newer molecular biological tests have generated interest but are not yet standardized or readily available in most clinical laboratory settings nor have they been validated in large clinical trials. Laboratory surveillance of at-risk patients could result in earlier initiation of antifungal therapy if sensitive and specific diagnostic tests, which are also cost effective, become available. This review will compare diagnostic tests currently in use as well as those under development by describing their assets and limitations for the diagnosis of invasive candidiasis.

• Archives of Pharmacal Research
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• v.8 no.3
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• pp.119-132
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• 1985

The apparent effectiveness of 1-naphthyl-N-methyl carbamate (carbaryl) against a wide variety of insects motivated the study of its mammalian toxicity. In this toxicological study of carbaryl, mature male rats inhaled carbaryl at a mean concentration of 112mg, 168mg and 224 mg/$m^{3}$ for one hour. After inhalation, pentobarbital sleeping time, Nadph-cytochrome c reductase activity, cytochrome p-450 and protein content in liver microsomes, various tissue residues, cholinesterase inhibition in plasma and histopathological findings at autopsy were observed. The pentobarbital sleeping time was prolonged in rats inhaled with carbaryl for one day while the sleeping time was shortened in the 3 days inhaled group. The changes of cytochrome p-450 content and NADPH-cytochrome c reductase activity exhibited biphasic response showing the decrease in the one day inhaled group and the increase in the 3 days inhaled group. The marked depression of plasma ChE activity was observed in rats inhaled with carbaryl at 112 mg/$m^{3}$, however no more progressive effect was observed at the higher concentration of the compound. The main observations in histopathological finding were ciliary detachment, epithelial swelling and subepithelial inflammatory cellular infiltration in trachea due to the irritation.

• Membrane and Water Treatment
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• v.8 no.2
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• pp.185-199
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• 2017

Low-pressure membrane filtration is increasingly used for tertiary treatment of wastewater effluent organic matter (EfOM), mainly comprising organic base/neutral compounds. In-line coagulation with underdosing, charge neutralization, and sweep floc conditions prior to ultrafiltration (UF) was studied to determine removals of the EfOM components and consequent reduction of fouling using polyethersulfone membranes. Coagulation and UF substantially reduced fouling for all coagulation conditions while removing from 7 to 38% of EfOM organic acids. From 7 to 16% of EfOM organic base/neutrals were removed at neutral pH but there was no significant removal for slightly acid coagulation conditions even though fouling was substantially reduced. Sweep floc produced the lowest resistance to filtration but may be inappropriate for in-line use due to the large added volume of solids. Charge-neutralization resulted in poor recovery of the initial flux with hydraulic cleaning. Under-dosing paralleled sweep floc in reducing hydraulic resistance to filtration (for sub-critical flux) and the initial flux was also easily recovered with hydraulic cleaning. Hydrophobic and hydrophilic base/neutrals were identified on the fouled membranes but as previously reported the extent of fouling was not correlated with accumulation of organic base/neutrals.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.13 no.6
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• pp.3258-3279
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• 2019

Recently, ransomware has earned itself an infamous reputation as a force to reckon with in the cybercrime landscape. However, cybercriminals are adopting other unconventional means to seamlessly attain proceeds of cybercrime with little effort. Cybercriminals are now acquiring cryptocurrencies directly from benign Internet users without the need to extort a ransom from them, as is the case with ransomware. This paper investigates advances in the cryptovirology landscape by examining the state-of-the-art cryptoviral attacks. In our approach, we perform digital autopsy on the malware's source code and execute the different malware variants in a contained sandbox to deduce static and dynamic properties respectively. We examine three cryptoviral attack structures: browser-based crypto mining, memory resident crypto mining and cryptoviral extortion. These attack structures leave a trail of digital forensics evidence when the malware interacts with the file system and generates noise in form of network traffic when communicating with the C2 servers and crypto mining pools. The digital forensics evidence, which essentially are IOCs include network artifacts such as C2 server domains, IPs and cryptographic hash values of the downloaded files apart from the malware hash values. Such evidence can be used as seed into intrusion detection systems for mitigation purposes.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• v.32 no.1
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• pp.3-9
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• 2021

Objectives: It is hard to accumulate research data on adolescents' suicide, because friends and family of the suicide completers might be reluctant to share the experience. To overcome the lack of information on adolescent suicide victims, the authors examined the risk and protective factors for adolescents' suicide from a character in a novel. Methods: Krystal, an adolescent female in the novel The Casual Vacancy by Joanne Rowling, failed to overcome her unfortunate circumstances and committed suicide. The authors analysed Krystal's case based on the guideline for patients with suicidal behaviours to address the complicated situation of her death. Results: Krystal grew up in a poor and dangerous environment. Despite the environmental hardships, she developed ego maturation with affectionate help from Mr Fairbrother, an assistant coach of the Girls' Rowing Team and a parish councillor. The sudden passing away of Mr Fairbrother brought on a crisis of identity for Krystal. In addition, a villainous character raped her and her brother drowned to death, which brought her great sorrow. She felt helpless and committed suicide. Conclusion: In spite of many risk factors for suicide, Krystal was able to keep her life with a few protective factors, a younger brother in the home, and a sense of responsibility for the family. After the loss of her brother, however, she collapsed in a moment. Krystal's suicide might not only be a personal choice but a breakdown of the social protection system for the youth.

• Journal of Pharmacopuncture
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• v.25 no.3
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• pp.268-275
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• 2022

Objectives: This toxicological study was performed to assess for potential toxicity and to determine the approximate lethal dose of SU-Eohyeol pharmacopuncture (SUEP) following a single intramuscular injection of SUEP into male and female Sprague-Dawley (SD) rats. Methods: The groups in our experiment consisted of an experimental group treated with SUEP at a dose of 1.0 mL/animal and a control group injected with a normal saline solution, and five male and female rats were placed in each group. Each animal was administered a single intramuscular injection. We monitored all rats for clinical signs and body weight changes for 14 days after administration. At the end of the observation period, the rats were euthanized and autopsied, and localized tolerance examinations were conducted at the site of administration of the test substance. Results: There were no deaths in either sex in the SUEP-treated group. There was no significant difference between the SUEP-treated group and the control group in the clinical signs and weight changes among the rats. In addition, no significant SUEP-related changes were observed on autopsy findings or local tolerance examinations at the injection site by histopathological examination. Conclusion: Our results suggest that the approximate lethal dose of a single intramuscular administration of SUEP in female and male rats under the conditions of this study is greater than 1.0 mL/animal. To determine the safety of the use of SUEP in Korean medical clinical practice, additional toxicity studies will be needed.