• Title/Summary/Keyword: Automatic synthesis

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Genome Analysis and Optimization of Caproic Acid Production of Clostridium butyricum GD1-1 Isolated from the Pit Mud of Nongxiangxing Baijiu

  • Min Li;Tao Li;Jia Zheng;Zongwei Qiao;Kaizheng Zhang;Huibo Luo;Wei Zou
    • Journal of Microbiology and Biotechnology
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    • v.33 no.10
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    • pp.1337-1350
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    • 2023
  • Caproic acid is a precursor substance for the synthesis of ethyl caproate, the main flavor substance of nongxiangxing baijiu liquor. In this study, Clostridium butyricum GD1-1, a strain with high caproic acid concentration (3.86 g/l), was isolated from the storage pit mud of nongxiangxing baijiu for sequencing and analysis. The strain's genome was 3,840,048 bp in length with 4,050 open reading frames. In addition, virulence factor annotation analysis showed C. butyricum GD1-1 to be safe at the genetic level. However, the annotation results using the Kyoto Encyclopedia of Genes and Genomes Automatic Annotation Server predicted a deficiency in the strain's synthesis of alanine, methionine, and biotin. These results were confirmed by essential nutrient factor validation experiments. Furthermore, the optimized medium conditions for caproic acid concentration by strain GD1-1 were (g/l): glucose 30, NaCl 5, yeast extract 10, peptone 10, beef paste 10, sodium acetate 11, L-cysteine 0.6, biotin 0.004, starch 2, and 2.0% ethanol. The optimized fermentation conditions for caproic acid production by C. butyricum GD1-1 on a single-factor basis were: 5% inoculum volume, 35℃, pH 7, and 90% loading volume. Under optimal conditions, the caproic acid concentration of strain GD1-1 reached 5.42 g/l, which was 1.40 times higher than the initial concentration. C. butyricum GD1-1 could be further used in caproic acid production, NXXB pit mud strengthening and maintenance, and artificial pit mud preparation.

Analysis of Delay Characteristics in Advanced Intelligent Network-Intelligent Peripheral (AIN IP) (차세대 지능망 지능형 정보제공 시스템의 지연 특성 분석)

  • 이일우;최고봉
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.25 no.8A
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    • pp.1124-1133
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    • 2000
  • Advanced Intelligent Network Intelligent Peripheral (AIN IP) is one of the AIN elements which consist of Service Control Point (SCP), Service Switching Point (SSP), and IP for AIN services, such as play announcement, digit collect, voice recognition/synthesis, voice prompt and receipt. This paper, featuring ISUP/INAP protocols, describes the procedures for call setup/release bearer channels between SSP/SCP and IP, todeliver specialized resources through the bearer channels, and it describes the structure and procedure for AIN services such as Automatic Collect Call (ACC), Universal Personal Telecommunication (UPT), and teleVOTing(VOT). In this environments, the delay characteristics of If system is investigated as the performance analysis, Policy establishment.

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Parametrized Construction of Virtual Drivers' Reach Motion to Seat Belt (매개변수로 제어가능한 운전자의 안전벨트 뻗침 모션 생성)

  • Seo, Hye-Won;Cordier, Frederic;Choi, Woo-Jin;Choi, Hyung-Yun
    • Korean Journal of Computational Design and Engineering
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    • v.16 no.4
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    • pp.249-259
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    • 2011
  • In this paper we present our work on the parameterized construction of virtual drivers' reach motion to seat belt, by using motion capture data. A user can generate a new reach motion by controlling a number of parameters. We approach the problem by using multiple sets of example reach motions and learning the relation between the labeling parameters and the motion data. The work is composed of three tasks. First, we construct a motion database using multiple sets of labeled motion clips obtained by using a motion capture device. This involves removing the redundancy of each motion clip by using PCA (Principal Component Analysis), and establishing temporal correspondence among different motion clips by automatic segmentation and piecewise time warping of each clip. Next, we compute motion blending functions by learning the relation between labeling parameters (age, hip base point (HBP), and height) and the motion parameters as represented by a set of PC coefficients. During runtime, on-line motion synthesis is accomplished by evaluating the motion blending function from the user-supplied control parameters.

Image Completion using Belief Propagation Based on Planar Priorities

  • Xiao, Mang;Li, Guangyao;Jiang, Yinyu;Xie, Li;He, Ye
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.10 no.9
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    • pp.4405-4418
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    • 2016
  • Automatic image completion techniques have difficulty processing images in which the target region has multiple planes or is non-facade. Here, we propose a new image completion method that uses belief propagation based on planar priorities. We first calculate planar information, which includes planar projection parameters, plane segments, and repetitive regularity extractions within the plane. Next, we convert this planar information into planar guide knowledge using the prior probabilities of patch transforms and offsets. Using the energy of the discrete Markov Random Field (MRF), we then define an objective function for image completion that uses the planar guide knowledge. Finally, in order to effectively optimize the MRF, we propose a new optimization scheme, termed Planar Priority-belief propagation that includes message-scheduling-based planar priority and dynamic label cropping. The results of experiment show that our approach exhibits advanced performance compared with existing approaches.

The Postprocessor of Automatic Segmentation for Synthesis Unit Generation (합성단위 자동생성을 위한 자동 음소 분할기 후처리에 대한 연구)

  • 박은영;김상훈;정재호
    • The Journal of the Acoustical Society of Korea
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    • v.17 no.7
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    • pp.50-56
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    • 1998
  • 본 논문은 자동 음소 분할기의 음소 경계 오류를 보상하기 위한 후처리 (Postprocessing)에 관한 연구이다. 이는 현재 음성 합성을 위한 음성/언어학적 연구, 운율 모델링, 합성단위 자동 생성 연구 등에 대량의 음소 단위 분절과 음소 레이블링된 데이터의 필요성에 따른 연구의 일환이다. 특히 수작업에 의한 분절 및 레이블링은 일관성의 유지가 어렵고 긴 시간이 소요되므로 자동 분절 기술이 더욱 중요시 되고 있다. 따라서, 본 논문은 자동 분절 경계의 오류 범위를 줄일 수 있는 후처리기를 제안하여 자동 분절 결과를 직접 합성 단위로 사용할 수 있고 대량의 합성용 운율 데이터 베이스 구축에 유용함을 기술한다. 제안된 후처리기는 수작업으로 조정된 데이터의 특징 벡터를 다층 신경회로망 (MLP:Multi-layer perceptron)을 통해 학습을 한 후, ETRI(Electronics and Telecommunication Research Institute)에서 개발된 음성 언어 번역 시스템을 이용한 자동 분절 결과와 후처리기인 MLP를 이용하여 새로운 음소 경계를 추출한다. 고립단어로 발성된 합성 데이터베이스에서 후처리기로 보정된 분절 결과는 음성 언어 번역 시스템의 분할율보 다 약 25%의 향상된 성능을 보였으며, 절대 오류(|Hand label position-Auto label position |)는 약 39%가 향상되었다. 이는 MLP를 이용한 후처리기로 자동 분절 오류의 범위를 줄 일 수 있고, 대량의 합성용 운율 데이터 베이스 구축 및 합성 단위의 자동생성에 이용될 수 있음을 보이는 것이다.

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ACOUSTIC FEATURES DIFFERENTIATING KOREAN MEDIAL LAX AND TENSE STOPS

  • Shin, Ji-Hye
    • Proceedings of the KSPS conference
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    • 1996.10a
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    • pp.53-69
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    • 1996
  • Much research has been done on the rues differentiating the three Korean stops in word initial position. This paper focuses on a more neglected area: the acoustic cues differentiating the medial tense and lax unaspirated stops. Eight adult Korean native speakers, four males and four females, pronounced sixteen minimal pairs containing the two series of medial stops with different preceding vowel qualities. The average duration of vowels before lax stops is 31 msec longer than before their tense counterparts (70 msec for lax vs 39 msec for tense). In addition, the average duration of the stop closure of tense stops is 135 msec longer than that of lax stops (69 msec for lax vs 204msec for tense). THESE DURATIONAL DIFFERENCES ARE 50 LARGE THAT THEY MAY BE PHONOLOGICALLY DETERMINED, NOT PHONETICALLY. Moreover, vowel duration varies with the speaker's sex. Female speakers have 5 msec shorter vowel duration before both stops. The quality of voicing, tense or lax, is also a cue to these two stop types, as it is in initial position, but the relative duration of the stops appears to be much more important cues. The duration of stops changes the stop perception while that of preceding vowel does not. The consequences of these results for the phonological description of Korean as well as the synthesis and automatic recognition of Korean will be discussed.

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Low Phase Noise Design and Implementation of X -Band Frequency Synthesizer for Radar Receiver (레이다 수신기용 X-밴드 주파수 합성기의 저 위상잡음설계 및 구현)

  • So, Won-Wook;Kang, Yeon-Duk;Lee, Taek-Kyung
    • Journal of Advanced Navigation Technology
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    • v.2 no.1
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    • pp.22-33
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    • 1998
  • In the coherent-on-receiver radar system using the magnetron source, frequency synthesizer is employed as a STALO(Stable Local Oscillator) to keep the intermediate frequency stable. In this paper, X-band(8.4GHz~9.7GHz) single loop frequency synthesizer is designed and implemented by an indirect frequency synthesis technique. Phase comparison is performed by a digital PLL(Phase-Locked Loop) chip and the loop filter is designed for the low phase noise. The effects of loop component characteristics on the output phase noise are analyzed for single loop structures, and the calculated results are compared with the measured data.

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Efficient Acoustic Echo Cancellation System for Distant-Talking Automatic Speech Recognition (원거리 음성 인식을 위한 효율적인 에코제거 시스템)

  • Kim, Ki-Beom;Kim, Sang-Yoon;Lee, Woo-Jung;Kwon, Min-Seok;Ko, Byeong-Seob
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2014.10a
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    • pp.150-155
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    • 2014
  • 본 논문에서는, 원거리 음성인식을 위한 서브밴드 필터링 기반의 빠르고 효율적인 에코제거 시스템을 제안한다. 제안하는 에코제거 시스템은 우선 채널간 유사도 (correlation) 가 높을 경우 적응필터가 오작동하는 것을 방지하기 위해 spatial decorrelation 을 적용하게 된다. 그리고 tree 형태를 가지는 IIR filterbank 기반의 subband 구조를 채택함으로써, 적은 차수로도 효과적인 analysis, synthesis 필터링을 수행할 수 있도록 한다. 이 과정에서 불가피하게 발생하는 서브 밴드간 spectral aliasing은 notch filter를 적용해 해결할 수 있다. 또한 적응 필터로는 improved proportionate normalized least-mean-square (IP-NLMS) 알고리즘을 사용해 수렴속도 및 에코제거 성능에서 우수함을 확인하였다. 마지막으로 decision-directed estimation 기반의 residual echo suppressor를 적용해 잔여 에코를 제거하게 된다. 본 논문에서는 각 단계를 구성하게 된 이론적인 배경을 소개하고, 실제 에코가 존재하는 환경에서 ERLE, 원거리 음성 인식률, computational complexity를 통해 제안하는 에코제거 시스템의 효과를 입증하도록 한다.

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Total Synthesis and Expression in E, coli of a Gene Coding for Human Interleukin-2 (인간 인터루킨-2를 코드하는 유전자의 합성과 대장균에서의 발현)

  • 주재훈;강성만;송인선;권종범;한문희;나도선
    • Microbiology and Biotechnology Letters
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    • v.19 no.3
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    • pp.228-234
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    • 1991
  • - A synthetic gene coding for human interleukin-2 (IL-2) was constructed from the oligonucleotides synthesized by an automatic DNA synthesizer. The nucleotide sequence of the synthetic gene was chosen considering the preferred codons of E. coEi by not changing the amino acid sequence of IL-2 polypeptide. The synthetic gene was expressed in E. coli by placing the gene under the control of the $\lambda$ PL promoter. IL-2 was produced in the E. coli cytoplasm in the form of inclusion bodies. The recombinant IL-2 showed growth promoting activity on the IL-2 dependent cell line.

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Study on the Vocabulary Synthesis for Index Term Selection (색인어 선정을 위한 어휘결집력에 관한 연구)

  • Kim, Chul;Jeong, Jun-Min
    • Journal of the Korean Society for information Management
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    • v.13 no.1
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    • pp.205-226
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    • 1996
  • Under the hypothesis that any pair of terms in the sentence is meaningful to present the context of the paper, the Brillouin measure of term relatedness in automatic indexing is proposed. For the experiment, the pair of terms simul-taneously appeared in two or more sentences of the paper are extracted from the title and abstract of the paper. Com-pared with the list of index terms or subject headings suggested by the author, the terms in term relatedness graph are highly matched with the terms in the list. Especially, it is revealed that the rank of terms by synthetic strength is use-ful in the selection of index terms.

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