• Applied Microscopy
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• v.51
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• pp.4.1-4.12
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• 2021

Fluorescence in situ hybridization (FISH) is a technique to visualize specific DNA/RNA sequences within the cell nuclei and provide the presence, location and structural integrity of genes on chromosomes. A confocal Whole Slide Imaging (WSI) scanner technology has superior depth resolution compared to wide-field fluorescence imaging. Confocal WSI has the ability to perform serial optical sections with specimen imaging, which is critical for 3D tissue reconstruction for volumetric spatial analysis. The standard clinical manual scoring for FISH is labor-intensive, time-consuming and subjective. Application of multi-gene FISH analysis alongside 3D imaging, significantly increase the level of complexity required for an accurate 3D analysis. Therefore, the purpose of this study is to establish automated 3D FISH scoring for z-stack images from confocal WSI scanner. The algorithm and the application we developed, SHIMARIS PAFQ, successfully employs 3D calculations for clear individual cell nuclei segmentation, gene signals detection and distribution of break-apart probes signal patterns, including standard break-apart, and variant patterns due to truncation, and deletion, etc. The analysis was accurate and precise when compared with ground truth clinical manual counting and scoring reported in ten lymphoma and solid tumors cases. The algorithm and the application we developed, SHIMARIS PAFQ, is objective and more efficient than the conventional procedure. It enables the automated counting of more nuclei, precisely detecting additional abnormal signal variations in nuclei patterns and analyzes gigabyte multi-layer stacking imaging data of tissue samples from patients. Currently, we are developing a deep learning algorithm for automated tumor area detection to be integrated with SHIMARIS PAFQ.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2019.05a
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• pp.157-158
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• 2019

Reinforcing bars are an important material for tensile strength of structures. For this reason, the inspection of the reinforcing bars confirming the layout and omission is very important for the safety of the structures. However, the current method of inspecting of the reinforcing bars through photographs of specific areas is difficult to identify condition all reinforcing bars. And It is also difficult to confirm after completion of a building. Therefore, reinforcing bar inspection using 3D scanner is required for automation of rebar inspection and database construction. For this purpose, this study test application of automated inspection method for rebar inspection using 3D scanner and discuss the effect of this method.

• Proceedings of the KSME Conference
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• 2001.06c
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• pp.134-139
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• 2001

Laser scanners are widely used for reverse engineering and inspection of freeform parts in industry such as motors, electronic products, dies and molds. Due to the lack of measuring software and positioning device, the laser scanning processes have been erroneous and inconsistent. In order to automate measuring processes, an automated scan plan generation software and a proprietary hardware are developed. In this paper, an automated laser scanning system using a 3-axis motorized stage is proposed. In the scan planning step, scan directions, paths, and the number of scans are generated considering optical and mechanical parameters. In the scanning step, the generated scan plan is downloaded into the laser scanner and the motorized stage and the points on the surface are captured automatically. Finally, the point data set is analyzed to evaluate the performance of the system.

• Journal of the Korean Society for Nondestructive Testing
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• v.23 no.1
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• pp.30-37
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• 2003

In order to replace the manual ultrasonic testing(UT) with an automated UT(AUT), a scanner which enables us to control the positions of a transducer is essential. Encoders have been commonly used to obtain the position information from the conventional scanners controlled by motor. Encoders have various advantages in many aspects. However, if the slip of motor wheel occurs during scanning, various errors are involved in the position accuracy. Thus, the position information of encoders becomes meaningless in case of slip. The reliability of AUT results nay become serious problem. Hence, slip must be avoided, but it can not be completely avoided at present time. In this paper, a new idea that surface wave is used to solve this problem and replace encoders has been proposed. It is shown that this idea can be employed in AUT scanner without encoders. That is, one transducer transmitting surface wave is fixed and the other transducer attached to the scanner receives UT signal. Then, computer calculates the present position of scanner based on the information given by surface wave. Thus, the movement of a scanner can be controlled by the amount of input based on the information obtained.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2004.10a
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• pp.929-934
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• 2004

As 3D scanner develops, it can be used in measurement. To accomplish complete 3D measurement, the scanner has to view different sides of the target. It can be done by moving the scanner and fix it at every measuring point. By human, it would take so much time. However, by using robot, measuring time can be reduced and the procedure can be automated. It is suitable for 6R serial manipulator to do this kind of work in which the scanner should go any position in arbitrary orientation. We did inverse kinematics analysis by analytical and graphical methods. Then, we compared two methods.

• Journal of Korean Tunnelling and Underground Space Association
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• v.8 no.4
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• pp.377-388
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• 2006

Most structures experience deterioration after construction. A routine inspection and maintenance must be accomplished for the efficient use of the structures. The routine inspection will play a major role on the determination of maintenance period and method. This study aims development of an automated tunnel inspection system based upon a 3 dimensional laser scanner. As for the initial stage of the project, a prototype tunnel scanner has been developed. The development of a tunnel scanner prototype follows comparison between image scanning and laser scanning system and investigation on the applicability and adaptivity of the scanners to the railway tunnel scanner. The applicability of the laser scanner on the railway tunnel has been confirmed from the pilot test by using commercialized general purpose close range laser scanner and applicability of a laser scanner as a railway tunnel scanner has been checked. From the result, a prototype of railway tunnel scanner has been built and the calibration of the system was carried out. Finally the developed tunnel laser scanner has been applied to different shapes and sizes of tunnels in use.

• International Journal of Precision Engineering and Manufacturing
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• v.1 no.1
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• pp.146-151
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• 2000

The importance of shape reconstruction is increasing in many areas such as RPD(Rapid Product Development) and reverse engineering. Typical data in these areas are mainly classified as the shape data measured by a laser scanner and the data extracted from the CT image. The goal of this research is to realize three-dimensional shape construction by showing a possible way to analyze input image data and reconstruct the original shape. Two main steps of the reconstructing process are obtaining cross-section data from image processing and linking loops between one slice and the next. Objects reconstructed in this way are compared with other objects using a laser scanner and modelled by commercially available software. The technique is expected to be used in reverse engineering applications and the object modeling with automated process.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 1996.11a
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• pp.167-176
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• 1996

Flaw classification and sizing are very essential issues in quantitative ultrasonic nondestructive evaluation of various materials and structures including weldments. For performing of these tasks in an automated fashion, we are developing an intelligent ultrasonic evaluation system with a multi-axis portable scanner which can do consistent and efficient acquisition and processing of ultrasonic flaw signals. Here we present our efforts to develop of this intelligent system including design of the portable scanner, acquisition and processing of ultrasonic flaw signals, display of pseudo 3-D image of flaws, and classification and sizing of flaws in weldments.

• Journal of Auto-vehicle Safety Association
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• v.9 no.3
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• pp.24-30
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• 2017

This paper presents the development of laser scanner based static obstacle detection algorithm for vehicle localization on lane lost section. On urban autonomous driving, vehicle localization is based on lane information, GPS and digital map is required to ensure. However, in actual urban roads, the lane data may not come in due to traffic jams, intersections, weather conditions, faint lanes and so on. For lane lost section, lane based localization is limited or impossible. The proposed algorithm is designed to determine the lane existence by using reliability of front vision data and can be utilized on lane lost section. For the localization, the laser scanner is used to distinguish the static object through estimation and fusion process based on the speed information on radar data. Then, the laser scanner data are clustered to determine if the object is a static obstacle such as a fence, pole, curb and traffic light. The road boundary is extracted and localization is performed to determine the location of the ego vehicle by comparing with digital map by detection algorithm. It is shown that the localization using the proposed algorithm can contribute effectively to safe autonomous driving.

• International Journal of Precision Engineering and Manufacturing
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• v.3 no.4
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• pp.15-23
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• 2002

With the development of a laser scanner of high precision and increased speed, reverse engineering becomes a key approach to reduce the time for the development of new products. But the modeling process is not so automated enough until now. Modeling in real workshops is usually performed by the experienced operators and it requires a skillful technique to get the resultant surface of high quality and precision. In this paper, a systematic solution is proposed to automate the free-form surface generation from the measured point data. Compatibility is imposed to the measured point data during input curve generation. And the compatibility of cross-sectional curve is also considered for the loft surface generation. The data in each step is produced in IGES file format to make an easy interface to other CAD/CAM software without any further data manipulation.