• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.3 no.4
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• pp.271-275
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• 1998

Controversial observations on the effect of pre-attached bacteria on the attachment of diatoms to artificial surfaces immersed in seawater have been made. Furthermore, it is not known whether or not pre-attached bacteria on artificial surfaces influence attachment of diatoms in natural seawater. In this study, we used various surfaces to which marine bacteria were pre-attached for different incubation periods. In the first experiment, glass slides were initially attached by marine natural bacteria with different exposure time (0-133 hr) and then immersed into seawater for a certain time period. The attachment of diatoms was not affected by the abundance of pre-attached bacteria (p > 0.05). The maximum abundance of attached diatoms was found on control surfaces, and the minimum abundance on surfaces where attached bacterial abundance was highest. In the second experiment, glass slides and acryl slides, either attached by marine natural bacteria for 6 days or coated by agar, were immersed in seawater. Untreated slides were also employed. On the surfaces of acryl slides with the most abundant attached bacteria ($5.4{\pm}0.02{\times}10^5\;cells\;cm^{-2}$), abundances of attached diatoms were less than those on untreated slides. On the surfaces of glass slides with bacterial abundance of $2.5{\pm}1.0{\times}10^5\;cells\;cm^{-2}$, however, abundances of attached diatoms were not different from those of untreated slides. On the agar-coated slides, the immigration rate and immigration coefficient were on average > 2 folds compared to other surfaces, indicating high rates of diatom attachment on mucilage simulated surfaces. Therefore, it seems that pre-attachment of bacteria is not prerequisite for the attachment of diatoms on artificial surfaces.

• The Journal of Korean Academy of Prosthodontics
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• v.40 no.5
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• pp.470-483
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• 2002

The purpose of this study was to investigate further the mechanics of failure of magnets used for denture retention. Dyna magnets were retrived from denture that had failed after 34 months of clinical use. The magnets were observed and sectioned in order to analyse with high resolution scanning electron microscope. From this study, corrosion behaviors of used magnetic attachment were analysed. The results were as follows ; 1. In Nd-Fe-B based magnetic materials, the erosion-corrosion was started at ununiformed part of stainless steel cover. 2. Corrosion was initiated at weared stainless steel surface and then magnetic materials were spalled by corrosive solution. 3. Spatting was occurred in Nd-Fe-B magnet materials due to corrosion products and then corrosion rate was increased drastically. 4. Corrosion started from ununiformed stainless steel surface as well as welded zone. In conclusion, the failure of magnets may occur by either breakdown of the welding or breakdown of the encapsulating material. So, it is considered that the corrosion problem of dental magnetic materials could be solved to some extent with surface treatment of dental magnetic materials.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.15 no.7 s.100
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• pp.799-805
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• 2005

This paper describes the development process of high stiffness body for ride and handling performance. High stiffness and light weight vehicle is a major target in the refinement of Passenger cars to meet customers' contradictable requirements between ride and handling performance and fuel economy This paper describes the analysis approach process for high stiffness body through the data level of body stiffness. According to the frequency band. we can suggest the design guideline about lg cornering static stiffness, torsional and lateral stiffness, body attachment stiffness. The ride and handling characteristic of a vehicle Is significantly affected by vibration transferred to the body through the chassis mounting points from front and rear suspension. It is known that body attachment stiffness is an important factor of ride and handling performance improvement. And high stiffness helps to improve the flexibility of bushing rate tuning between handling and road noise. It makes possible to design the good handling performance vehicle and save vehicles to be used in tests by using mother car at initial design stage. These improvements can lead to shortening the time needed to develop better vehicles.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2004.11a
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• pp.358-361
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• 2004

This paper describes the development process of high stiffness body for ride and handling performance. High stiffness and light weight vehicle is a major target in the refinement of passenger cars to meet customers' contradictable requirements between ride and handling performance and fuel economy. This paper describes the analysis approach process for high stiffness body through the data level of body stiffness. According to the frequency band, we can suggest the design guideline about Is cornering static stiffness, torsional and lateral stiffness, body attachment stiffness. The ride and handling characteristic of a vehicle is significantly affected by vibration transferred to the body through the chassis mounting points from front and rear suspension. It is known that body attachment stiffness is an important factor of ride and handling performance improvement. And high stiffness helps to improve the flexibility of bushing rate tuning between Handling and road noise. It makes it possible to design the good handling performance vehicle at initial design stage and save vehicles to be used in tests by using mother car at initial design stage. These improvements can lead to shortening the time needed to develop better vehicles.

• The Korean Journal of Malacology
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• v.19 no.2
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• pp.107-110
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• 2003

The effect of broodstock age on the spawning performance of cultured abalone, Haliotis discus hannai, was assessed for five age groups (3, 4, 5, 6, 7 years old). Spawning performance of the five age groups was assessed in terms of the percentage of spawning abalone, eggs per spawning female, fertilization rates, hatch rates and attachment rates per spawning. The percentage of spawning abalone increased along with broodstock age, reached maximum in female groups of 5- and 6-year age (60%), and in male group of 6-year age (100%), subsequently decreased in 7-year age group. The number of eggs produced per spawning also increased with broodstock age, and the maximum was found in 6-year age. Although the number of spawned eggs for the oldest abalone declined, it still spawned much more eggs than 3, 4, and 5 years old females. The fertilization and hatch rates were obviously larger for 4-6 years old group than the younger and older abalones. The maxima were found in 5-year age group (96.0% and 84.5%), and the minimum were appeared in 7-year age group (79.3% and 58.2%). The attachment rate increased with broodstock age, reached maximum in 6-year age group (33.6%), although the increment gradually declined. The attachment rate for 7-year age group rapidly decreased (16.6%). These results suggested that broodstock age affected the spawning performance of H. discus hannai, which peaked between 5 and 6 years old, and broodstock should be bred during this period for hatchery production.

• Journal of Environmental Science International
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• v.21 no.1
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• pp.105-111
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• 2012

The effects of substrate size on the growth of microphytobenthos Achnanthes sp., Amphora sp., Navicula sp. and Nitzschia sp. were examined using glass beads in order for phytoremediation in the benthic layer of coastal waters. The glass beads used in this study were 0.09~0.15 mm (G.B 1), 0.25~0.50 mm (G.B 2), 0.75~1.00 mm (G.B 3) and 1.25~1.65 mm (G.B 4). No addition of glass bead used as control. The specific growth rate and maximum cell density of four microphytobenthos species were increasing with decreasing size of glass beads. Moreover, the control experiment without added attachment substrates showed the lowest specific growth rate and maximum cell density. Therefore, the suitable attachment substrates for mass culture of microphytobenthos seems to be important in order for phytoremediation using microphytobenthos.

• Fisheries and Aquatic Sciences
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• v.17 no.3
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• pp.385-390
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• 2014

Abalone immortal cell lines can be used to study the physiological properties and disease mechanisms of abalone at the cellular and molecular level. As a first step for the final goal to establish abalone immortal cell lines, we examined various initial culture conditions for primary cell populations derived from Haliotis discus hannai radula tissue. The survival rate after cell isolation procedures using the enzymatic method was as low as $9.95{\pm}2.37%$. Based on three different experimental conditions for H. discus hannai radula-derived cell culture, we found that the salinity of the media and the presence of growth-promoting factors were important to support radula-derived primary cell populations during the initial culture. The growth factor-containing media adjusted to 35 psu salinity could induce 100% (8 out of 8 trials) initial cell attachment, and the rate of cell attachment reached 50-70%. The data obtained from this study will provide useful information for developing immortal cell lines from abalone species.

• Journal of Aquaculture
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• v.18 no.3
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• pp.142-146
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• 2005

In this study, the reattachment processes of small and medium size of spot of Mytilus coruscus and M. edulis were observed. The small spats (Shell length, SH, $3{\sim}7mm$) of hard shelled mussel, M. coruscus showed 100% reattachment rate within 7 minutes after the detachment of byssus thread and 100% survival rate. While large spats (SH 25 mm) showed 85% reattachment rate within 5 hours and 100% survival rate for 24 hours. The reattachment rate of M. edulis (SH 30 mm) was higher than that of M. coruscus (SH, 28 mm). The thread consisted of three identifiable structures; adhesive disc, adhesive thread and adhesive root. The adhesive disk and adhesive thread of M. coruscus were larger and thicker than those of M. edulis, whereas the thickness of adhesive root was the other way round. Further studies are required to identify the relationship between the structure of byssus thread and attachment ability. This study suggests that the sizes of spats could be an important factor in determining the timing of removing and reattaching mussel seedling for aquaculture or releasing to the sea.

• The Journal of Korean Academy of Prosthodontics
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• v.42 no.2
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• pp.175-191
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• 2004

Statement of problem: Platelet-rich plasma(PRP) is well known to be very effective method to stimulate and accelerate the healing of bone and soft tissue. However, there are few reports which deal with the mechanisms of the PRP on the activation of the osteoblasts. Purpose: This study was aimed to investigate the effect of growth factors in PRP on the activity of osteoblasts. Material and method: To evaluate the effect on human, human osteoblast cell line was cultured. PRP was extracted from the blood of a healthy volunteer. Using the recombinant growth factors of PDGF, $TGFT-\beta$, IGF-1, bFGF which are mainly found at bone matrix and their neutralizing antibody, the effect of PRP on the attachment and proliferation of osteoblasts was evaluated. To evaluate the autocrine and paracrine effects, conditioned media(CM) of PRP was made and compared with PRP. By the western blot analysis, the expression of growth factors in PRP, CM was examined. Cell morphology was compared by the light microscope. Results : 1) The effects of CM on osteoblast were similar to the effects of PRP. 2) PRP, CM, recombinant $TGF-\beta$, bFGF, IGF-1 showed significantly higher cellular attachment than control(p<0.05) in the cell attachment assay. In the cell proliferation assay, PRP, CM, recombinant $TGF-\beta$, IGF-1, bFGF, PDGF increased significantly cell proliferation(p<0.01). Among the recombinant growth factors, IGF-1 showed the highest cellular attachment and proliferation. 3) In the western blot assay, bFGF, IGF-1, PDGF weve equally expressed in PRP and CM. 4) The attachment of osteoblast cell decreased significantly after the addition of neutralizing antibody against $TGF-\beta$, IGF-1(p<0.05). In the cell proliferation assay, the addition of neutralizing antibody against $TGF-\beta$, bFGF, PDGF, IGF-1 decreased significantly the cellular proliferation(p<0.05). The amount of decreasing in the cell attachment and proliferation is the highest in at-lGF-1. 5) The cells in control group were flattened and elongated with a few cellular processes in the a light microscope. But, the cells appeared as spherical, plump cells with well developed cellular processes in experimental groups. The cells in PRP and CM had more prominent developed features than recombinant growth factor groups. Conclusions : These findings imply that PRP maximize the cellular activity in early healing period using the synergistic effect, autocrine, paracrine effects of growth factors and increase the rate and degree of bone formation.

• Journal of Periodontal and Implant Science
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• v.31 no.2
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• pp.489-499
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• 2001

Bone graft and guided tissue regeneration have been used for the regeneration of periodontal tissue which is the ultimate goal of periodontal treatment. Recently, it was reported that some kind of growth factors were used for regeneration. Platelet rich plasma was researched that it could increase the density of bone and the rate of bone regeneration. For that, 25 patients which have pocket depth more than 5mm at any of 6 surfaces, of healthy patient without any systemic disease were treated. $Biogran^{?}$ Were grafted into 14 infrabony pockets as controls, and $Biogran^{(R)}$ with PRP were inserted into 31 infrabony pockets. And then, follwing evaluations were made at the end of 1, 3 and 6 months. 1. There was no statistical difference between control and experimental group in pocket depth, gingival recession, minimum probing attachment level and maximum probing attachment level at preoperation(p>0.05). 2. Decrease in probing pocket depth were reduced to 3.32mm for experimental group and 2.71mm for control group. The decrease was evident at the end of 1 month, they were 2.97mm and 2.29mm,and it was statistically difference(p<0.05). 3. Gingival recession was increased by 0.55mm in experimental group and 0.50mm in control group, it was evident at the end of 1 month. And it was statistically difference(p<0.05). 4. Minimum probing attachment level was increased by 0.35mm in experimental group and 0.36mm in control group, it was statistically difference(p<0.05). 5. Maximum probing attachment level was decreased by 3.19mm in experimental group and 2.93mm in control group, it was statistically difference(p<0.05). 6. There was no statistical difference between control and experimental group in pocket depth, gingival recession, minimum probing attachment level and maximum probing attachment level(p>0.05). There was statistical difference in decrease of pocket depth between pre-operation and 1 month after post-operation(p<0.05). In conclusion, bone graft using $Biogran^{?}$ and bone graft using $Biogran^{?}$ With platelet rich plasma were both effective in treatment of infrabony pocket, bone graft using $Biogran^{?}$ With platelet rich plasma was more effective in early soft tissue healing.