• 제목/요약/키워드: Atmospheric temperature and humidity

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생체 모사수 화장품이 세포 활성과 피부에 미치는 효과 (Effect of Cosmetics Contained Isotonic Water Mimicked Body Fluid on Cell Activities and Skin)

  • 박선영;이성훈;김은주;최소웅;김지영;조성아;조준철;이해광
    • 대한화장품학회지
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    • 제40권2호
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    • pp.195-201
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    • 2014
  • 생체수는 링거액, 인공관절액, 세포 배양 등 다양한 분야에서 연구되어 왔다. 이는 생체수가 체온을 조절하는데 결정적인 역할을 하고, 생체 내 다양한 대사 과정에서 용매로 사용되며, 삼투압이나 능동적 섭취를 통한 혈액 또는 림프액을 통해 세포에서 세포로 전달되는 미네랄, 에너지원, 호르몬, 시그널과 약물의 전달 물질로 이용되기 때문이다. 세포외 지질과 자연보습성분(natural moisturizing factor, NMF)을 함유하는 각질층은 외부의 수분을 끌어들여 내부 수화에 이용하며, 이러한 과정들은 피부 장벽 기능과의 연관성이 높다. 본 연구에서는 피부 장벽 기능을 강화하는 아미노산, 펩타이드, 단당류를 포함한 생체모사수(Cell Bio Fluid SyncTM)를 인체 피부에 처리하여 세포활성을 관찰하고, 생체 모사수를 함유한 제품을 피부에 도포하여 인체피부 개선 효과를 연구 하였다. 피부 세포 활성을 보기 위해 각질세포인 HaCaT 세포를 이용하였고, 에너지 고갈상태로 만들기 위해 3시간 동안 PBS에 전 처리한 후 이후 세포 배양액인 DMEM 및 등장액인 PBS, 생체 모사수(Cell Bio Fluid $Sync^{TM}$)에 각각 3시간 처리하였다. 이후 MTT assay와 이미지 분석을 수행하였다. 인체의 피부 개선 효능을 위한 임상 연구에 21명의 여성이 참여하였다. 생체 모사수를 함유한 제품을 1주일간 안면에 도포하였고, 수분량, 피부결, 밝기 그리고 피부 균일도를 측정하였다. 모든 측정은 피험자가 세안 후 항온항습조건($22{\pm}2^{\circ}C$, $50{\pm}5%$)에서 20분 간 대기하고 수행하였다. 모든 데이터는 SPSS ver. 21을 이용하여 분석하였다. 그 결과, 생체 모사수(Cell Bio Fluid $Sync^{TM}$)가 세포 활성을 높이고 인체의 피부에서 수분, 거칠기, 밝기, 균일도, 투명도 등의 개선 효과가 있음을 확인할 수 있었다.

한국산(韓國産) 왕대나무의 현존량(現存量)과 토양(土壤) 미세균류상(微細菌類相) (The Standing Crops and Soil-borne Microfungal Flora of Phyllostachys reticulata in Korea)

  • 김관수
    • 한국균학회지
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    • 제7권2호
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    • pp.91-116
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    • 1979
  • 본(本) 연구(硏究)는 예산지역(禮山地域)(A)과 광산지역(光山地域)(B)의 왕대나무(Phyllostachys reticulata)의 현존량(現存量)과 그 토양(土壤) 미세균류상(微細菌類相)을 조사(調査)한 것이다. 왕대나무의 죽림밀도(竹林密度)는 예산지역(禮山地域)이 17,250본(本)/ha이며 광산지역(光山地域)이 14,780본(本)/ha으로서 예산지역(禮山地域)이 16.1%가 많았다. 양지역(兩地域)의 환경요인(環境要因)은 B지역(地域)의 생장기간(生長期間)의 평균기온(平均氣溫)이 A지역(地域)보다 $1.5{\sim}2^{\circ}C$가 높았고 토양온도(土壤溫度)도 $1{\sim}2^{\circ}C$가 높았으며 토양내(土壤內)에 함유(含有)되어 있는 전실소(全室素), 인산(燐酸) 및 유기물질량(有機物質量)도 약간(若干)많았다. 또 B지역(地域)에서는 낙엽량(落葉量)과 부식량(腐植量) 그리고 죽림내(竹林內)의 식생수량(植生數量)도 많았으며 죽림지(竹林地)에서 환원(還元)되는 각종(各種) 유기물(有機物) 분해(分解)에 관여(關與)하는 미세균류(微細菌類)도 Mortierella elongata, Mucor circinelloides, Aspergillus japonicus, Penicillium waksmani and Trichoderma lignorum등의 5종(種)이 더 많았다. 온도(溫度)는 죽림내부(竹林內部)로 들어 갈수록 낮았고 습도(濕度)는 높았다. 죽림내(竹林內)의 상대조도(相對照度)의 비율(比率)은 A지역(地域)이 4.19% B지역(地域)이 2.7%로서 하단분(下端部)에서는 모두 광합성작용(光合成作用)을 할 수 있는 능력(能力)이 상실(喪失)되었으나 조도(照度)가 약(弱)할수록 표토(表土)근처에서 서식(棲息)하는 미세균류(微細菌類)는 오히려 활동력(活動力)을 강(强)하게 하는 것으로 생각한다. 생산구조도(生産構造圖)에서 B지역(地域)의 광합성부(光合成部)의 최대량(最大量)이 대나무 지상부(地上部)의 상단(上端)에 위치(位置)하고 있어서 높은 생산량(生産量)을 유지(維持)하는데 효과적(效果約)인 구조(構造)였다고 생각된다. A,B지역(地域) 죽림(竹林)에서 $D^2H$, $w_s,\;w_b$, 및 $w_l$ 상대생장식(相對生長式)을 유도(誘導)한 결과(結果)는 다음과 같다. A지역(地域) $logw_s=0.5262\;logD^2H+1.9546$ $logw_b=0.6288\;logD^2H+1.5723$ $logw_l=0.5181\;logD^2H+1.8732$ B지역(地域) $logw_s=0.5433\;logD^2H+1.8610$ $logw_b=0.1630\;logD^2H+2.3475$ $logw_l=0.4509\;logD^2H+2.0041$ 상기(上記)한 식(式)을 적용(適用)하여 10a당 현존량(現存量)을 조사(調査)한 결과(結果) A지역(地域) $w_s=1128.83kg,\;w_b=689.05kg,\;w_l=926.69kg$ 으로 $w_e=2744.57kg$이었고, B지역(地域) $w_s=1206.66kg,\;w_b=679.92kg,\;w_l=1112.51kg$으로 $w_t=2999kg$이였다. 따라서 A,B양지역간(兩地域間)에 있어서 $D^2H,\;w_s,\;w_b$, 및 $w_l$의 현존량(現存量)을 비교한 결과(結果)(t-test), $D^2H,\;w_s,\;w_b,\;w_l$에서는 유의차(有意差)가 인정되었으나 $w_b$는 유의차(有意差)가 없었다. 토양생(土壤生) 미세균류(微細菌類)를 조사(調査)한 결과(結果) 158균주(菌株)를 분리(分離)하고 55종(種)을 동정(同定)하였다. 그 중 A지역(地域) 50종(種), B지역(地域) 55종(種)으로 양지역(兩地域)의 우점종(優占種)들은 다음과 같다. Trichoderma viride, Penicillium janthinellum, P. commune, Aspergillus oryzae, A. niger, A. gigantus, A. fumigatus, Mortierella ramaniana, var. anguliFPora, Mucor hiemalis와 Zygorhynchus moelleri. 이상(以上)의 결과(結果)에 의(依)하면 토성(土性)이 좋고 토양양료(土壤養料) 및 토양생(土壤生) 미세균류(微細菌類)의 증가(增加) 그리고 생육기간(生育期間)의 온도(溫度)가 왕대나무의 생장(生長)이나 임상식물(林床植物)의 종(種)과 양(量)을 증가(增加)시킨 것으로 나타났고 왕대나무의 현존량(現存量)과 환경요인(環境要因)과의 상관관계(相關關係)는 이들의 모든 요인(要因)이 상호연관(相互連關)을 갖고 복잡(複雜)하게 작용(作用)한 것으로 보며 더욱 죽림밀도(竹林密度)가 중용(重要)한 인자(因子)로 작용(作用)한 것같다.

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논벼 장.단간품종의 증발산제계수와 건물량과의 관계에 대한 연구(I) (Studies on Relations between Various Coeffcients of Evapo-Transpiration and Quantities of Dry Matters for Tall-and Short Statured Varieties of Paddy Rice)

  • 류한열;김철기
    • 한국농공학회지
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    • 제16권2호
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    • pp.3361-3394
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    • 1974
  • The purpose of this thesis is to disclose some characteristics of water consumption in relation to the quantities of dry matters through the growing period for two statured varieties of paddy rice which are a tall statured variety and a short one, including the water consumption during seedling period, and to find out the various coefficients of evapotranspiration that are applicable for the water use of an expected yield of the two varieties. PAL-TAL, a tall statured variety, and TONG-lL, a short statured variety were chosen for this investigation. Experiments were performed in two consecutive periods, a seedling period and a paddy field period, In the investigation of seedling period, rectangular galvanized iron evapotranspirometers (91cm${\times}$85cm${\times}$65cm) were set up in a way of two levels (PAL-TAL and TONG-lL varieties) with two replications. A standard fertilization method was applied to all plots. In the experiment of paddy field period, evapotanspiration and evaporation were measured separately. For PAL-TAL variety, the evapotranspiration measurements of 43 plots of rectangular galvanized iron evapotranspirometer (91cm${\times}$85cm${\times}$65cm) and the evaporation measurements of 25 plots of rectangular galvanized iron evaporimeter (91cm${\times}$85cm${\times}$15cm) have been taken for seven years (1966 through 1972), and for TONG-IL variety, the evapotranspiration measurements of 19 plots and the evaporation measurements of 12 plots have been collected for two years (1971 through 1972) with five different fertilization levels. The results obtained from this investigation are summarized as follows: 1. Seedling period 1) The pan evaporation and evapotranspiration during seedling period were proved to have a highly significant correlation to solar radiation, sun shine hours and relative humidity. But they had no significant correlation to average temperature, wind velocity and atmospheric pressure, and were appeared to be negatively correlative to average temperature and wind velocity, and positively correlative to the atmospheric pressure, in a certain period. There was the highest significant correlation between the evapotranspiration and the pan evaporation, beyond all other meteorological factors considered. 2) The evapotranpiration and its coefficient for PAL-TAL variety were 194.5mm and 0.94∼1.21(1.05 in average) respectively, while those for TONG-lL variety were 182.8mm and 0.90∼1.10(0.99 in average) respectively. This indicates that the evapotranspiration for TONG-IL variety was 6.2% less than that for PAL-TAL variety during a seedling period. 3) The evapotranspiration ratio (the ratio of the evapotranspiration to the weight of dry matters) during the seedling period was 599 in average for PAL-TAL variety and 643 for TONG-IL variety. Therefore the ratio for TONG-IL was larger by 44 than that for PAL-TAL variety. 4) The K-values of Blaney and Criddle formula for PAL-TAL variety were 0.78∼1.06 (0.92 in average) and for TONG-lL variety 0.75∼0.97 (0.86 in average). 5) The evapotranspiration coefficient and the K-value of B1aney and Criddle formular for both PAL-TAL and TONG-lL varieties showed a tendency to be increasing, but the evapotranspiration ratio decreasing, with the increase in the weight of dry matters. 2. Paddy field period 1) Correlation between the pan evaporation and the meteorological factors and that between the evapotranspiration and the meteorological factors during paddy field period were almost same as that in case of the seedling period (Ref. to table IV-4 and table IV-5). 2) The plant height, in the same level of the weight of dry matters, for PAL-TAL variety was much larger than that for TONG-IL variety, and also the number of tillers per hill for PAL-TAL variety showed a trend to be larger than that for TONG-IL variety from about 40 days after transplanting. 3) Although there was a tendency that peak of leaf-area-index for TONG-IL variety was a little retarded than that for PAL-TAL variety, it appeared about 60∼80 days after transplanting. The peaks of the evapotranspiration coefficient and the weight of dry matters at each growth stage were overlapped at about the same time and especially in the later stage of growth, the leaf-area-index, the evapotranspiration coefficient and the weight of dry matters for TONG-IL variety showed a tendency to be larger then those for PAL-TAL variety. 4) The evaporation coefficient at each growth stage for TONG-IL and PAL-TALvarieties was decreased and increased with the increase and decrease in the leaf-area-index, and the evaporation coefficient of TONG-IL variety had a little larger value than that of PAL-TAL variety. 5) Meteorological factors (especially pan evaporation) had a considerable influence to the evapotranspiration, the evaporation and the transpiration. Under the same meteorological conditions, the evapotranspiration (ET) showed a increasing logarithmic function of the weight of dry matters (x), while the evaporation (EV) a decreasing logarithmic function of the weight of dry matters; 800kg/10a x 2000kg/10a, ET=al+bl logl0x (bl>0) EV=a2+b2 log10x (a2>0 b2<0) At the base of the weight of total dry matters, the evapotranspiration and the evaporation for TONG-IL variety were larger as much as 0.3∼2.5% and 7.5∼8.3% respectively than those of PAL-TAL variety, while the transpiration for PAL-TAL variety was larger as much as 1.9∼2.4% than that for TONG-IL variety on the contrary. At the base of the weight of rough rices the evapotranspiration and the transpiration for TONG-IL variety were less as much as 3.5% and 8.l∼16.9% respectively than those for PAL-TAL variety and the evaporation for TONG-IL was much larger by 11.6∼14.8% than that for PAL-TAL variety. 6) The evapotranspiration coefficient, the evaporation coefficient and the transpiration coefficient and the transpiration coefficient were affected by the weight of dry matters much more than by the meteorological conditions. The evapotranspiratioa coefficient (ETC) and the evaporation coefficient (EVC) can be related to the weight of dry matters (x) by the following equations: 800kg/10a x 2000kg/10a, ETC=a3+b3 logl0x (b3>0) EVC=a4+b4 log10x (a4>0, b4>0) At the base of the weights of dry matters, 800kg/10a∼2000kg/10a, the evapotranspiration coefficients for TONG-IL variety were 0.968∼1.474 and those for PAL-TAL variety, 0.939∼1.470, the evaporation coefficients for TONG-IL variety were 0.504∼0.331 and those for PAL-TAL variety, 0.469∼0.308, and the transpiration coefficients for TONG-IL variety were 0.464∼1.143 and those for PAL-TAL variety, 0.470∼1.162. 7) The evapotranspiration ratio, the evaporation ratio (the ratio of the evaporation to the weight of dry matters) and the transpiration ratio were highly affected by the meteorological conditions. And under the same meteorological condition, both the evapotranspiration ratio (ETR) and the evaporation ratio (EVR) showed to be a decreasing logarithmic function of the weight of dry matters (x) as follows: 800kg/10a x 2000kg/10a, ETR=a5+b5 logl0x (a5>0, b5<0) EVR=a6+b6 log10x (a6>0 b6<0) In comparison between TONG-IL and PAL-TAL varieties, at the base of the pan evaporation of 343mm and the weight of dry matters of 800∼2000kg/10a, the evapotranspiration ratios for TONG-IL variety were 413∼247, while those for PAL-TAL variety, 404∼250, the evaporation ratios for TONG-IL variety were 197∼38 while those for PAL-TAL variety, 182∼34, and the transpiration ratios for TONG-IL variety were 216∼209 while those for PAL-TAL variety, 222∼216 (Ref. to table IV-23, table IV-25 and table IV-26) 8) The accumulative values of evapotranspiration intensity and transpiration intensity for both PAL-TAL and TONG-IL varieties were almost constant in every climatic year without the affection of the weight of dry matters. Furthermore the evapotranspiration intensity appeared to have more stable at each growth stage. The peaks of the evapotranspiration intensity and transpiration intensity, for both TONG-IL and PAL-TAL varieties, appeared about 60∼70 days after transplanting, and the peak value of the former was 128.8${\pm}$0.7, for TONG-IL variety while that for PAL-TAL variety, 122.8${\pm}$0.3, and the peak value of the latter was 152.2${\pm}$1.0 for TONG-IL variety while that for PAL-TAL variety, 152.7${\pm}$1.9 (Ref.to table IV-27 and table IV-28) 9) The K-value in Blaney & Criddle formula was changed considerably by the meteorological condition (pan evaporation) and related to be a increasing logarithmic function of the weight of dry matters (x) for both PAL-TAL and TONG-L varieties as follows; 800kg/10a x 2000kg/10a, K=a7+b7 logl0x (b7>0) The K-value for TONG-IL variety was a little larger than that for PAL-TAL variety. 10) The peak values of the evapotranspiration coefficient and k-value at each growth stage for both TONG-IL and PAL-TAL varieties showed up about 60∼70 days after transplanting. The peak values of the former at the base of the weights of total dry matters, 800∼2000kg/10a, were 1.14∼1.82 for TONG-IL variety and 1.12∼1.80, for PAL-TAL variety, and at the base of the weights of rough rices, 400∼1000 kg/10a, were 1.11∼1.79 for TONG-IL variety and 1.17∼1.85 for PAL-TAL variety. The peak values of the latter, at the base of the weights of total dry matters, 800∼2000kg/10a, were 0.83∼1.39 for TONG-IL variety and 0.86∼1.36 for PAL-TAL variety and at the base of the weights of rough rices, 400∼1000kg/10a, 0.85∼1.38 for TONG-IL variety and 0.87∼1.40 for PAL-TAL variety (Ref. to table IV-18 and table IV-32) 11) The reasonable and practicable methods that are applicable for calculating the evapotranspiration of paddy rice in our country are to be followed the following priority a) Using the evapotranspiration coefficients based on an expected yield (Ref. to table IV-13 and table IV-18 or Fig. IV-13). b) Making use of the combination method of seasonal evapotranspiration coefficient and evapotranspiration intensity (Ref. to table IV-13 and table IV-27) c) Adopting the combination method of evapotranspiration ratio and evapotranspiration intensity, under the conditions of paddy field having a higher level of expected yield (Ref. to table IV-23 and table IV-27). d) Applying the k-values calculated by Blaney-Criddle formula. only within the limits of the drought year having the pan evaporation of about 450mm during paddy field period as the design year (Ref. to table IV-32 or Fig. IV-22).

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