• Ocean Science Journal
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• v.40 no.3
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• pp.177-182
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• 2005

The process of embryogenesis and larval development of the asteroid sea star Asterias amurensis $(U{\ddot{u}}tken)$ was observed, with special attention paid to morphological change and larval duration. In reproductive season, mature sea stars were collected under floating net cages, located in Tongyeong, southern Korea. The mature eggs are $138\;{\mu}m$ in average diameter, semi-translucent and orange in color, sperms in good condition appear light cream to white-gray in color. Embryos develop through the holoblastic equal cleavage stage and a wrinkled blastula stage that lasts about 9 hours after fertilization. Gastrulae bearing an expanded archenteron hatch from the fertilization envelope 22 hours after fertilization. At the end of gastrulation, rudiments of the left and right coelom are formed. By day 2, larvae possess complete alimentary canal and begin to feed. At this stage, the larva is called early bipinnaria. In 6-day-old larvae, the pre- and post- oral ciliated bands form complete circuits and the bipinnarial processes start to develop. By day 12, the lateral and anterior projection of the larval wall processes along the ciliated bands begins to thicken and curl, and the ciliated bands become more prominent. By day 32, early brachiolaria are presented with three pairs of brachiolar arms. Advanced brachiolaria with a well-developed brachiolar complex (three pairs of brachia and central adhesive disc) occur 6 weeks after fertilization. In the field, spawning of the sea star was observed in April to May, settlement form larvae and just settlements seem to occur from June to July, and early juveniles occur from August to September. Although we had not described the end of brachiolaria stage, it can be tentatively estimated that the duration of the pelagic stage of A. amurensis is 40 to 50 days.

• Proceedings of the Korean Society of Computer Information Conference
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• 2013.07a
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• pp.307-310
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• 2013

아무르불가사리에 기인한 환경적, 경제적 피해는 해마다 증가되고 있습니다. 우리나라 남해와 동해 바다에 널려 있는 불가사리들이 최근 서해안에서도 급속히 늘어나고 있습니다. 특히 러시아 해역에 서식하는 아무르 불가사리의 개체수가 급증해 어장을 황폐화 시키고 있습니다. 이로 인하여 엄청난 번식력과 포식력을 지닌 아무르불가사리가 살아있는 조개류를 먹어치우고 있어서 어민들의 피해가 막심합니다. 이 논문은 변형되어진 아무르불가사리의 구조적 특징과 방사대칭 특성을 이용하여 불가사리 영상을 복원하는 복원기법으로 불가사리의 인식률을 높게 하여 불가사리 퇴치에 많은 도움이 될 것으로 기대합니다.

• Proceedings of the Korean Society of Computer Information Conference
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• 2010.07a
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• pp.213-216
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• 2010

불가사리로 인한 어업 피해액은 연간 100억원에 이른다. 전복과 조개 등 어패류를 먹어치우는 불가사리는 강한 번식력에 어장 황폐화의 원인이 되고 있다. 불가사리는 해양 생태계에 뚜렷한 천적이 없고 세계적으로 불가사리의 개체수가 늘어나고 있는 것으로 추정하고 있다. 아무르불가사리는 단일 색상이 아니고 상황에 따라 변하는 변온동물이며, 방사대칭인 특징은 있지만 상황에 따라 외형이 많이 달라지므로 인식하기가 매우 어렵다. 이 논문은 아무르불가사리의 인식을 높이기 위하여 여러 개의 아무르불가사리의 개체들이 있을 때에 아무르불가사리로 인식하기 위한 간단한 기법을 제안하고자 한다.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.10 no.5
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• pp.83-88
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• 2010

This study is going to suggest the technology to recognize a starfish by judging various starfish images. In case of recognition of single objects of the asterias amurensis, a starfish can be judged by using concave features and short line features but in case of multiple objects, it is impossible to extract the features of a starfish using concave features or short line so that it can't be recognized as a starfish. Accordingly, it is going to suggest the recognition technology using the features such as numbers of standard deviation, relative degree standard deviation and valid deviation of a long line by using the region central moment and a long line of multiple objects. As a result of experiments of the suggested technology, there were cases that recognition failed because the conditions of the standard deviation of a long line or the numbers of valid deviation of the relative degree couldn't satisfy the conditions but around 95% of a high recognition rate was shown.

• Fisheries and Aquatic Sciences
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• v.12 no.1
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• pp.1-5
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• 2009

Crude saponin, extracted from the starfish Asterias amurensis, was assessed for its capacity as a biological pesticide. As part of this analysis, its insecticidal and repellent activities, in addition to its acute and chronic toxicities were tested. In comparison with the control group, insecticidal activity of saponin against tobacco cutworm, Spodoptera litura, in kale, Brassica loeracea was low at 36.4%. Repellent activities of the extracted saponin against green peach aphid, Myzus persicae, and S. litura, on soybean leaf, Glycine max and kale were 65.6% and 35.0% at $1^{st}$ day, and 54.5% and 30.0% at $3^{rd}$ day, respectively. Acute and chronic toxicity analysis was carried out using acute immobilizatioin test and reproduction impairment test, respectively. The saponin had 48 h-$EC_{50}$ of $65.21{\mu}g/mL$. Twenty-one day accumulative reproduction after treatment was lower in Daphnia magna at $7{\mu}g/mL$ saponin (78 youngs), compared with the control group (129 youngs). These results indicate that the extracted saponin exhibited some toxicity and has potential as a repellent against insects.

• Korean Journal of Food Science and Technology
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• v.39 no.6
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• pp.625-629
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• 2007

This study was carried out to investigate the anti-wrinkle effects of peptides derived from collagens isolated from Asterias amurensis, which was collected in the East Sea. The molecular weights of the peptides were between 10-50 kDa, as determined through sephadek G-75 gel. The cytotoxicities against CCD-986sk cells and HEL-299 cells were measured using the MTT assay. The cytotoxicity of all the fractions(F1: Fraction No. 4-13, 116 kDa; F2: Fraction No. 25-30, 100 kDa; F3: Fraction No. 45-55, 58 kDa; F4: Fraction No. 59-63, 43 kDa; F5: Fraction No. 79-90, 24 kDa) was less than 25%, by the addition of 1.0 mg/mL. These peptides did not show any adverse effects on human skin cells. In the presence of F1 at 1.0 mg/mL, matrix metalloproteinase-1 (MMP-1) expression of UVA-induced human normal fibroblasts was reduced to 34.8%. Overall, the results seem to suggest that peptides of approximately 20 kDa have superior anti-wrinkle effects.

• Proceedings of the Korean Aquaculture Society Conference
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• 2004.05a
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• pp.379-380
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• 2004

• Korean Journal of Food Science and Technology
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• v.42 no.4
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• pp.424-430
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• 2010

Immuno-modulatory activities of peptides from Asterias amurensis were investigated using a nano-encapsulation process. The molecular weights of the peptides in the range of 5-7 kDa were separated using Sephadex G-75 gel filtration. Eighty-five percent of the nano-particles were in the 300 nm range using dynamic light scattering. The cytotoxicity of the A. amurensis nano-particles against CCD-986sk human dermal fibroblast cells was 11.64% after adding 1.0 mg/mL of the samples, which was lower than that from the control (13.28% collagen). The secretion of $NO^-$ from macrophages was estimated as $40\;{\mu}M$ after adding 1.0 mg/mL of gelatin nano-particles, which was higher than the others. Prostaglandin $E_2$ production from UV-induced human skin cells decreased greatly to 860 pg/mL after adding 1.0 mg/mL of the samples. Confocal microscopy revealed that nano-particles effectively penetrated the cells within 1 hour. From these results, we consider that nano-encapsulation of the peptides from A. amurensis can improve their biological functions.

• Korean Journal of Food Science and Technology
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• v.40 no.5
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• pp.522-527
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• 2008

Low molecular peptides were isolated from Asterias amurensis via SDS-PAGE. The peptides were separated via consecutive gel filtration as five fractions (F1-F5) according to molecular weights, based on the results of MALDI-TOF MS analysis. The molecular weight of the most active peptide was estimated as 15,000 daltons. The peptide showed cytotoxicity on normal human fibroblast cells at levels as low as 20% when 1.0 mg/mL of the samples was added. The peptide also exhibited higher levels of nitric oxide production from macrophages than the lipopolysaccaharides. It was determined that prostaglendin $E_2$ production was significantly inhibited, up to 127.8% as compared to the control. The low molecular peptide inhibited hyaluronidase activity as 535.7 ${\mu}g/mL$ of $IC_{50}$. It can be concluded that the relatively low molecular weight peptide, fucoidan, from A. amurensis has excellent cosmetic and immunomodulatory activities, which can be considered as a possible resource of new cosmetic agents for skin immunomodulation.

• Journal of Fisheries and Marine Sciences Education
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• v.24 no.6
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• pp.781-792
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• 2012

Seasonal variation of species composition in marine organisms around Oryukdo of Busan was investigated using a gill net between 2009 and 2011. A total of 82 species, 52 families, and 6 taxa were collected during the sampling periods. Species were included two species in Cnidaria, one in Urochordata, 12 in Mollusca, 12 in Crustacea, seven in Echinodermata, and 48 in Pisces. The dominant species were Crepidula onyx, Asterina pectinifera, Ceratostoma burnetti, Asterias amurensis, Anthocidaris crassispina, and Trachurus japonicus. These six species accounted for 48.4% in the total number. Diversity indices were highest in spring 2009 (2.77) and lowest in spring 2011 (1.66). Seasonal variation of species composition and abundance in marine organisms corresponded with the water temperature.