• Asian Pacific Journal of Cancer Prevention
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• v.16 no.13
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• pp.5199-5203
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• 2015

Background: Helicobacter pylori (H.pylori) is associated with chronic gastritis, peptic ulcers, gastric adenocarcinomas and mucosa associated tissue lymphomas. Cytotoxin associated gene A (CagA) is one of the virulence factors of H.pylori. It is hypothesized that reactive oxygen species (ROS) play roles in H.pylori associated disease especially in development of gastric adenocarcinoma. Individuals infected with H.pylori bearing CagA produce more ROS than others. 8-hydroxydeoxyguanosine (8OHdG) is an in vitro marker of DNA damage and oxidative stress. The aim of this study was to investigate the relationship between 8OHdG level, H.pylori infection and CagA and alterations of serum 8OHdG level after H.pylori eradication. Materials and Methods: Patients admitted with dyspeptic complaints and upper gastrointestinal endoscopy were assessed. H.pylori was determined from histopathology of specimens. Serum 8OHdG levels of three groups (H.pylori negative, H. pylori positive CagA negative and H.pylori positive CagA positive) were compared. Patients with H.pylori infection received eradication therapy. Serum 8OHdG levels pretreatment and posttreatment were also compared. Results: In total, 129 patients (M/F, 57/72) were enrolled in the study. Serum 8OHdG level of H.pylori negative, H. pylori positive CagA negative and H.pylori positive CagA positive groups were significantly different ($5.77{\pm}1.35ng/ml$, $5.43{\pm}1.14ng/ml$ and $7.57{\pm}1.25ng/ml$ respectively, p=0.05). Furthermore, eradication therapy reduced serum 8OHdG level ($6.10{\pm}1.54ng/ml$ vs $5.55{\pm}1.23ng/ml$, p=0.05). Conclusions: Individuals infected with H.pylori bearing CagA strains have the highest serum 8OHdG level and eradication therapy decreases the serum 8OHdG level. To the best of our knowledge this is the first study that evaluated the effect of CagA virulence factor on serum 8OHdG level and the effect of eradication therapy on serum 8OHdG levels together. Eradication of CagA bearing H.pylori may prevent gastric adenocarcinoma by decreasing ROS. 8OHdG level may thus be a good marker for prevention from gastric adenocarcinoma.

• Journal of Microbiology and Biotechnology
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• v.17 no.6
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• pp.993-1001
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• 2007

The technique of serological analysis of antigens by recombinant cDNA expression library (SEREX) uses autologous patient sera as a screening probe to isolate tumor-associated antigens for various tumor types. Isolation of tumor-associated antigens that are specifically reactive with patient sera, but not with normal sera, is important to avoid false-positive and autoimmunogenic antigens for the cancer immunotherapy. Here, we describe a selection methodology to isolate patient sera-specific antigens from a yeast surface-expressed cDNA library constructed from 15 patient lung tissues with non-small cell lung cancer (NSCLC). Several rounds of positive selection using patient sera alone as a screening probe isolated clones exhibiting comparable reactivity with both patient and normal sera. However, the combination of negative selection with allogeneic normal sera to remove antigens reactive with normal sera and subsequent positive selection with patient sera efficiently enriched patient sera-specific antigens. Using the selection methodology described here, we isolated 3 known and 5 unknown proteins, which have not been isolated previously, but and potentially associated with NSCLC.

• Hwankyungkyoyuk
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• v.25 no.2
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• pp.161-179
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• 2012

The aims of this study are to investigate the environmental perception of elementary school students through picture drawing and associated words about environment, and analyze whether there are differences in the environmental perception according to living places and grades. The drawing of 230 students are analysed and categorized into the criteria. The results are as follows: a number of students realized as images associated with natural environment for words related to environment. In the pictures of the 'positive environment', expression frequencies of natural environment and living things were higher than ones of artificial environment and non-living things. On the other hand, there were many pictures expressed as pollutions of waste, air and water in the pictures of the 'negative environment'. Comparison of environmental perception between rural and urban students showed a significant difference all in the two criteria. There was, however, no difference in the environmental perception between grades. Therefore, it is suggested that student's minds about the sentimental situation can be studied and they can be guided in positive directions to lead their future life in environment-friendly education rather than pollution education.

• Korean Journal of Health Education and Promotion
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• v.13 no.1
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• pp.28-44
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• 1996

The purpose of this study is to examine the associated risk factors for adolescent cigarette smoking. In February 1995, a total of 1793 students from 17 general high schools in Seoul were assessed with a self-completing questionnaire pertaining current smoking status and school.family.peer environmental factors. The results are as follows; 1. The overall proportion of current smoker among students in the study was 17.3%; 27.7% in males and 6.6% in females. 2. There was significant association between smoking status and all school environmental factors (ranks at school, satisfaction at school, study hours after school, extracurricular activity) examined. 3. Siblings smoking in males and family structure in females were significantly associated with the smoking status of students. 4. Functional aspect of family environmental factors, such as APGAR score, parental supervision, attachment to father or to mother were related to smoking status of students. 5. Association with friends who smoke was significantly associated with smoking status of students. 6. In males, association with friends who smoke, ranks at school, siblings smoking were significant positive predictors and APGAR score, parental supervision, attchment to mother were significant negative predictors. In females, association with friends whosmoke, ranks at school, satisfaction at school were significant positive predictors and parental supervision, attchment to father were significant negative predictors. These findings suggest that strategies that influence smoking behavior need to be directed not only to the individual student but also to their peer group, family and school environment.

• IMMUNE NETWORK
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• v.21 no.4
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• pp.31.1-31.16
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• 2021

Gastric cancer (GC) is the fourth most common cause of cancer-related death globally. The classification of advanced GC (AGC) according to molecular features has recently led to effective personalized cancer therapy for some patients. Specifically, AGC patients whose tumor cells express high levels of human epidermal growth factor receptor 2 (HER2) can now benefit from trastuzumab, a humanized monoclonal Ab that targets HER2. However, patients with HER2^negative AGC receive limited clinical benefit from this treatment. To identify potential immune therapeutic targets in HER2^negative AGC, we obtained 40 fresh AGC specimens immediately after surgical resections and subjected the CD45⁺ immune cells in the tumor microenvironment to multi-channel/multi-panel flow cytometry analysis. Here, we report that HER2 negativity associated with reduced overall survival (OS) and greater tumor infiltration with neutrophils and non-classical monocytes. The potential pro-tumoral activities of these cell types were confirmed by the fact that high expression of neutrophil or non-classical monocyte signature genes in the gastrointestinal tumors in The Cancer Genome Atlas, Genotype-Tissue Expression and Gene Expression Omnibus databases associated with worse OS on Kaplan-Meir plots relative to tumors with low expression of these signature genes. Moreover, advanced stage disease in the AGCs of our patients associated with greater tumor frequencies of neutrophils and non-classical monocytes than early stage disease. Thus, our study suggests that these 2 myeloid populations may serve as novel therapeutic targets for HER2^negative AGC.

• Journal of the Korean Mathematical Society
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• v.41 no.5
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• pp.883-894
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• 2004

We discuss in this paper the strong convergence for weighted sums of negative associated (in abbreviation: NA) arrays. Meanwhile, the central limit theorem for weighted sums of NA variables and linear process based on NA variables is also considered. As corollary, we get the results on iid of Li et al. ([10]) in NA setting.

• Honam Mathematical Journal
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• v.34 no.2
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• pp.209-217
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• 2012

Convergence rates in the law of large numbers for i.i.d. random variables have been generalized by Gut[Gut, A., 1978. Marc inkiewicz laws and convergence rates in the law of large numbers for random variables with multidimensional indices, Ann. Probab. 6, 469-482] to random fields with all indices having the same power in the normalization. In this paper we generalize these convergence rates to the identically distributed and negatively associated random fields with different indices having different power in the normalization.

• Journal of applied mathematics & informatics
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• v.6 no.3
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• pp.959-968
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• 1999

In this paper we are obtained new results for bivariate pro-cesses which help us to tell the dependent structure among hitting times of the processes. We are proposed both dependence properties and the-oretical results among the processes and certain kinds of dependence properties when we are imposed on processes are reflected as analo-gous properties of corresponding hitting times. Finlly we are given some examples to illustrate these concepts.

• Communications of the Korean Mathematical Society
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• v.22 no.2
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• pp.315-321
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• 2007

We show an exponential inequality for negatively associated and strictly stationary random variables replacing an uniform boundedness assumption by the existence of Laplace transforms. To obtain this result we use a truncation technique together with a block decomposition of the sums. We also identify a convergence rate for the strong law of large number.

• Korean Journal of Health Education and Promotion
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• v.28 no.4
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• pp.39-49
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• 2011

Objectives: This study was to examine the association between structural and functional characteristics of social network and self-rated health in middle-aged Korea population. We also explored gender difference in the relationship between social network and health. Methods: Data were collected from individuals aged 40-69 years old participating in the 2005 survey for the Korean Genome & Epidemiology Study. We examined the association between social network, social support, social conflict and self-rated health using multiple logistic regression analysis stratified by gender. Results: The extent and contact frequency of close people, and social participations were associated by not only the positive function but also the negative function of social network. Both the positive and negative functions of social network affected self-rated health. The relationship between the function of social network and health showed a gender difference: only positive function was significantly associated with health in men while only negative function had significant relationship with health in women. Conclusions: Social support and social conflict affected the health in both genders through different ways. The ambivalent effect of social network on health should be explored further.