• YAKHAK HOEJI
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• v.39 no.5
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• pp.548-553
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• 1995

Effects of restraint stress and its modulations by ursodeoxycholic acid(UDCA) were evaluated on some biochemical and biophysical parameters in rats. Restraint stress induced elevations in blood alkaline phosphatase (ALP). cholesterol (CHOL), aspartate transaminase (GOT), alanine transaminase (GPT), lactate dehydrogenase (LDH) levels. It was also caused adrenal hypertrophy, decrease in weight of spleen and contents of ascorbic acid in stressed rats. As a results, stress indicators such as spleen, ascorbic acid, GOT, GPT, LDH were fastly changed after imposing stress, but those such as ALP, CHOL, adrenal were induced relatively later. UDCA was tested if it has an inhibitory effect against 18-hr restraint induced stress. UDCA lowered ALP, CHOL, LDH level and also effectively elevated the ascorbic acid contents in 25 mg/kg dosage of UDCA. In organ weights. the restraint stress induced increases in spleen and adrenal were attenuated by UDCA in 50 mg/kg dosage. However. stress-induced GOT and GPT levels were unaffected by UDCA.

• Korean Journal of Soil Science and Fertilizer
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• v.7 no.4
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• pp.209-213
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• 1974

The isozymes of glutamic oxalacetic transaminase from young rice root were isolated by DEAE-cellulose column chromatography, and investigated some of their enzymic properties. Obtained results were summarized as follows: 1. 93 percent of GOT activity in rice root existed in supernatant fraction, and their specific activity exibited the same pattern. 2. The rice root contained two types of GOT isozyme; cationic and anionic GOT. It seemed that both of them were distributed in supernatant fraction. 3. GOT isozymes of rice root on the pH dependance showed their maximum activity at 7.4. 4. On the stability of GOT isozymes for temperature, anionic GOT was more unstable than cationic GOT. 5. Apparent Michaelis constant for L-aspartate of GOT isozymes from rice root showed 12-14mM in the cationic GOT and 16mM in the anionic GOT, respectively.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.11
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• pp.273-282
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• 2020

This study analyzed the physiological effects of silkworms and silkworm pupae on the reduction of serum lipid levels. Research data specific to silkworms and their pupae published over the last 10 years were collected and analyzed. A large effect size of 0.852 was observed in the overall results and the range of 95% confidence intervals was 0.662 to 0.999. The Q value was 23.264, the Higgin's I² value was 92.217, indicating that each study was heterogeneous. There was no publication bias, as the corrected observed number of statistically significant effect sizes and the expected number of statistically significant effect sizes were both 0.654. The silkworm study showed a large effect size with respect to blood sugar and blood lipid reduction at 0.801 and 0.948, respectively, and LDL-cholesterol reduction at 1.371, showing a very large effect size. Aspartate transaminase / glutamic oxaloacetic transaminase (AST/GOT) was 0.768, alanine transaminase /glutamate-pyruvate transaminase (ALT/GPT) was 0.788, which was a medium effect size. The experiment period had a very large effect size of 1.170 for five to eight weeks and 1.020 for an intake of 1 to 50 mg/kg. The silkworm pupae had a large effect size of 0.991 and 0.951, respectively on blood lipids and total cholesterol reduction. The experimental period showed a very large effect size at 1.103 for more than nine weeks, and the intake showed a large effect size at 0.855 for 5001 mg/kg.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.3
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• pp.204-210
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• 2014

The brown alga Saccharina japonica is consumed as a foodstuff in many countries. Carbon tetrachloride ($CCl_4$) is a potent hepatotoxin that is used to assess hepatotoxicity in animal models. This study assessed the protective effect of S. japonica extract (SJE) on $CCl_4$-induced acute liver injury in rats. Experimental rats were divided into the following three groups: control, $CCl_4$, and $CCl_4$+SJE; the latter two groups were given 150 or 300 mg SJE/kg orally for 10 days. Three hours after the final treatment, all rats-except for those in the control group-were administered intraperitoneal injections of $CCl_4$. One day later, blood and liver samples were collected for evaluation biomarker of levels. Aspartate transaminase (APT; GPT) and alanine aminotransferase (ALT; GOT) levels were markedly lower in the $CCl_4$+SJE group than the $CCl_4$ group. The hepatic superoxide dismutase (SOD) activity of the $CCl_4$+SJE group was significantly lower than that of the $CCl_4$ group. In comparison, glutathione S-transferase (GSH) and catalase (CAT) levels were significantly higher in the $CCl_4$+SJE group. Western blotting revealed that SJE attenuated the $CCl_4$-induced EGFR and MAPK activity in the liver. Thus, we conclude that SJE protects against $CCl_4$-induced hepatotoxicity.