• 한국축산식품학회지
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• 제38권6호
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• pp.1196-1202
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• 2018

The objective of this study was to determine the antioxidant activity of gamma-irradiated Asparagus cochinchinensis (Asparagi radix) (Lour.) Merr. Extract (ARE) and its inhibition effect on food lipid oxidation using emulsion-type pork sausage as a model. ARE was prepared from dried Asparagi radix root and ARE solution (1.0 g/mL) was gamma-irradiated with designated doses at 5, 10, and 20 kGy. Antioxidant activity of ARE solution was determined by measuring 1,1-diphenyl-e-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-9-sulphonic acid) (ABTS) radicals. Activities of DPPH and ABTS radicals were decreased, whereas total phenolic contents increased after gamma irradiation with a dose dependence. Addition of gamma-irradiated ARE dose-dependently retarded lipid oxidation of emulsion-type pork sausage during storage at $4^{\circ}C$. These results indicated that gamma-irradiated ARE might have antioxidant activity more than non-irradiated ARE due to increase of the content of polyphenolic compounds by ionizing radiation.

• 동의생리병리학회지
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• 제19권5호
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• pp.1391-1398
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• 2005

The anti-thrombic properties of the oriental herbal medicine Daejowhan(DJW, 大造丸) which consists of 11 kinds of herbs (indicated as ratio) of Rehmanniae Radix 24%, Hominis Placenta 5%, Testudinis Carapax 9%, Eucommiae Cortex 9%, Asparagi Radix 9%, Phellodendri Cortex 9%, Achyranthis Radix 7%, Liriopis Tuber 7%, Angelicae Sinensis Radix 7%, Ginseng Radix 5% and Schizandrae Fructus 3% were investigated. The water extracts from DJW inhibited Platelet-activating factor(PAF) induced platelet aggregation. DJW was extracted with methanol and further fractionated by ethylacetate. A 70% methanol extract showed a strong inhibition against PAF-induced aggregation in vitro and in vivo assays. The ethylacetate soluble fraction was shown to have inhibitory effect on PAF-induced platelet aggregation in vitro assay. The ethylacetate soluble fraction specially protected against the lethality of PAF, while verapamil did not afford any protection. These results indicate that the water extracts and alcoholic-fractions inhibit the action of PAF in vivo by an antagonistic effect on PAF, so that it may be useful in treating disorders caused by PAF, such as acute allergy, inflammation, asthma, gastrointestinal ulceration, toxic shock and so forth. DJW was investigated regarding its assumed anti-thrombic action on human platelets which was deduced from its ability to suppress Arachidonic acid(AA)-induced aggregation, exocytosis of ATP, and inhibition of Cyclooxygenase(COX) and Thromboxane synthase(TXS) activity. The latter two effects were estimated from the generation of Prostaglandin $E_2(PGE_2)$ and Thromboxane $A_2(TXA_2)$ respectively. Exogenously applied AA ($100{\mu}mol/{\ell}$) provoked a $89\%$ aggregation of platelets, the release of 14 pmol ATP, and the formation of either 225 pg $TXA_2$ or 45 pg $PGE_2$, each parameter being related to 106 platelets. An application of DJW 5 min before AA dose-dependently diminished aggregation, ATP-release and the synthesis of $TXA_2$ and $PGE_2$ with $IC_{50}$ values of 74, 108, 65, $72{\mu}g/m{\ell}$, respectively. The similarity of the $IC_{50}$ values suggest an inhibition of COX by DJW as primary target, thus suppressing the generation of $TXA_2$ which induces aggregation of platelets and exocytosis of ATP by its binding on $TXA_2$-receptors.

• 사상체질의학회지
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• 제12권1호
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• pp.216-227
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• 2000

1. 연구목적 태음인(太陰人) 청심연자탕(淸心蓮子湯)은 "동의수세보원(東醫壽世保元)" 태음인병 24처방 중에 기재된 신정방으로 태음인 간조열증(肝燥熱證)에 응용되는 처방이다. 근래에는 뇌졸중을 비롯한 중추신경계통 질환에 많이 사용되고 있다. 이러한 태음인(太陰人) 청심연자탕(心蓮子湯)의 용도를 과학기기를 이용하여 기전을 규명하고자 하였다. 2. 연구방법 백서를 urethane으로 마취시키고 태음인(太陰人) 청심연자탕(淸心蓮子湯)을 투여한 후 Pressure Transducer와 Laser-Doppler flowmetry를 이용하여 백서의 혈압과 국소뇌혈류량을 측정하였으며 이를 구성약물까지 진행하였다. 또한 태음인(太陰人) 청심연자탕(淸心蓮子湯)의 대뇌혈류에 관한 기전 규명을 위하여 propranolol과 methylene blue로 전처치한 후 태음인(太陰人) 청심연자탕(淸心蓮子湯)을 투여하여 이를 분석하였다. 3. 결과 및 결론 태음인(太陰人) 청심연자탕(淸心蓮子湯)의 투여로 혈압의 유의한 변화는 없었으며, 국소뇌혈류량은 유의하게 증가되었다. 구성약물 중 연자육(蓮子肉), 맥문동(麥門冬), 천문동(天門冬), 원지(遠志), 산조인(酸棗仁), 룡안육(龍眼肉), 나복자, 감국(甘菊)은 국소뇌혈류량을 유의하게 증가시켰다. 이 실험으로 태음인(太陰人) 청심연자탕(淸心蓮子湯)의 국소뇌혈류량 증가의 기전은 교감신경 ${\beta}$-수용체와 cyclic GMP의 생성효소인 guanylyl cyclase의 작용과 관련이 있는 것으로 생각된다.

• Journal of Periodontal and Implant Science
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• 제25권2호
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• pp.386-396
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• 1995

Interieukin 1${\beta}$ is a potent bone resorptive cytokine which mediates soft tissue destruction through the stimulatidn of prostaglandin production and the induction of collagenase. This constellation of activities suggests a role of IL-1${\beta}$ in the pathogenesis of periodontal disease. The purpose of this study was to evaluate the effects of herbal extracts on production and activity of IL-1${\beta}$. When LPS was added to cultured human blood monocytes, the effects of herbal extracts on the production of IL-1${\beta}$ was evaluate by thymocyte stimulation assay. When rHuIL-1${\beta}$ was added to cultured human gingival fibroblasts, the effects of herbal extracts on production of $PGE_2$ was evaluated by ELISA and when it was added to cultured mouse calvaria, the effects on bone resorption was estimated by .$^{45}Ca$-release bone resorption assay. The herbal extracts that had been used in this study were as follows; Asparagi Radix, Schzandrae Fractus, Zizyphi Fractus and Rhois Galla. The following results were obtained from this study. 1. All these extracts effectively inhibited the production of IL-1${\beta}$ on cultured human blood monocytes. 2. All these extracts effectively inibited the production of $PGE_2$ on cultured human gingival fibroblasts. 3. All these extracts did not effectively inhibit the bone resorption induced by rHulL-1${\beta}$ on cultured mouse calvaria.