• 농업과학연구
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• 제2권1호
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• pp.241-255
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• 1975

This experiment was conducted to observe the effects of fermented feed by Aspergillus oryzae and Aspergillus niger on the improvement of feed value and the effect of fermented feed additive for meat production of broiler. The results of fermented feed on the improvement of feed value were as follows; I; The effects of fermented feed value improvement were as follaws; 1) There were little difference between fermented feeds by Asp. oryzae and Asp. niger, compared with wheat bran, crude protein contents of Koji was highly increased and its nitrogen free extract and crude fat contents were decreased, but crude fiber and ash were little difference. 2) Total amino acids were highly increased as to fermented feeds but proline in Asp. niger koji feed, and proline and valine in Asp. oryzae koji feed were decreased and other amino acid were increased 2) The effect of fermented feeds on meat production of broiler were as follows; 1) Fermented feeds groups appeared higher weight (p<0.01)than weight of control on end of experimental period, but little difference were recognized between 5% and 10% fermented feed groups. 2) On the weight gain per day, highly significant were recognized(p<0.05) between control and test groups, 10% Asp. oryzae koji group was highest ($12.15{\pm}0.46g$) between all groups. 3) On the yield of carcass, there were significant highly difference (p<0.01) between control and test groups but little difference were recognized between each of 5% groups and 10% groups of fermented feeds. 4) Fermented feed groups appeared higher carcass yield (p (0<0.05) than control. But between all fermented feed groups were a little difference in partly. 5) On the influence of fowl meat composition, amount of moisture contents was a little decrease in fermented feed groups, and crude protein and crude fat were increased. 6) Feed conversion rate resulted a little amount decreasing. Specially, 10% Asp oryzae koji group was lowest (2.89) compare with control (3.35) 3. As a result of economical analysis appeared highest low income in koji groups. Low income were more gained percent of 40.22 in 10% Asp oryzae koji and 33.19 in 10% Asp. niger koji than control.

• 한국미생물·생명공학회지
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• 제19권2호
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• pp.140-146
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• 1991

Mutation experiments were performed to select the mutant of Aspergillus niger 55, which had lost almost all the ability to produce transglucosidases but retained that of high productivity of raw meal saccharifying enzyme, by means of successive induction with N-methyl-N'-nitro-N-nitrosoguanidine(MNNG), ultraviolet(UV) light, and ${\gamma}$-rays. Also, we used the mutant enrichment techniques, such as liquid culture-filtration procedure and differential heat sensitivity of conidia, in order to increase the possibility of obtaining a mutant. The glucoamylase productivity of mutant PFST-38 was 11 times higher than that of the parent strain. The mutant PFST-38 was morphologically identical to the parent strain, except for the size of conidia, the tendency to form conidia and the lenght of conidiophore. Asp. niger mutant PFST-38 apeared to be useful for the submerged production of the raw corn meal saccharifying enzyme.

• 한국미생물·생명공학회지
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• 제21권1호
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• pp.13-17
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• 1993

만다린 오렌지 과피를 기질로 하여 Asp. niger의 구연산 발효를 행하여 관련된 일련의 효소적 활성을 합성배지와 비교한 결과 만다린 오렌지 과피배지에서는 괴피에 함유된 Pectin이나 조섬유 등의 자화로 인하여 Polygalacturonase와 Pectin의 활성 뿐만 아니라 CMCase, xylannase 및 amylase의 활성이 높게 나타났다.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 1976년도 제8회 학술발표회
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• pp.190.4-190
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• 1976

Naringinase 생산 균주로 분리 선정된 Asp. niger S-1은 동시에 Hesperidinase도 강력히 생산함이 확인 되었으며 이 균의 효소학적 특성을 요약하여 1. 최적 반응 온도는 $60^{\circ}C이며$ $80^{\circ}C에서$ 30분 열처리 하여도 65%을 활성을 가지며 pH 5.0부위에서 최적반응과 안정성을 보였으며 Mg(이온)은 반응을 활성화 하였다. 2. Aceton을 60% 처리하여 조효소를 11배 정제하였으며 35%가 회수되었고 유안 0.4-0.6 포화로 48배 정제되었으며 13%가 회수되었다.

• 미생물학회지
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• 제18권1호
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• pp.1-6
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• 1980

Five species of food decay fungl, Aspergillus flavus, Asp. uiger, Penicillum sp., Botrytis cinerea, and Rhizopus stolonifer, were examined for their radio sensitivity in several suspension media. Asp. flavus, Asp. niger and Penicillum sp. have almost the same sensitivity toward gamma rays. with D value in the range of 30 to 35 K rad, whereas Botrytis cinerea has a D value of approximately 55K rad and Rhizopus stolonifer, the most re4sistant fungus studied, has a crease in their radioresistnace when compared with spores irradiated in water. Asp. flavus and penicillium sp. spores irradiated in citrate buffer at pH3-7 showed almost no change in their radisensitiity with pH, but Botryis cinerea spores showed a distinct decrease in their radioresistnace at pH 6 and 7. Penicillum sp. spores irradiated in sucrose solutions showed no sinificant change in their radioresistance. Botrytis cinerea spores displayed a higher radioresistance when they were irradiated in sucrose solution than in water.

• 미생물학회지
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• 제14권1호
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• pp.17-24
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• 1976

Celluloytic microorgnasims were isolated form the various sources and four of them were identified as Trichoderma roningi, Aspergillus niger, Penicillium chrysogenum, and Streptomyces sp. The induction of extracellular5 cellulase of these species in the liquid culture media containing carboxymethylcellulose (CMC) or Avicel as inducer showed that CMC was a better effective inducer for the production of CMCase(Cx cellulase component) as well as Avicelase(C$_{1}$ cellulase component) than Avicel. It is believed that certain hydrolysis products of cellulose(CMC) could serve as an inducer for an enzyme synthesis. In T. roningi, Asp. niger, and Strptomyces sp., the optimum temperature of CNCase on CMC-culture medium was 50.deg. but temperature around 40.deg.C was found to be optimum for the activities of CMCase prepared from P.ehrysogenum. The optimum temperature for Avicelase activitiles on Avicel-culture media of T. roningi and P. chrysogenum was $40{\circ}C$ whereas temperature $50{\circ}C$ was found to be optimum for Avicelase from A.niger and Streptomyces sp. The optimal activities of these CNCase and Avicelase prepared from. T. ronigi, Pen.chrysogenum and Streptomyces sp. were found similarly to be at pH's around 5.4 and 6.0 while pH 4.8 was optimum for the activities of CMCase and Avicelase from A.niger, indicating that A.niger in acidic media would yield an enzyme of high activity.

• 한국미생물·생명공학회지
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• 제6권4호
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• pp.161-165
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• 1978

자연계에서 유기산을 생성하는 균주 11 군주를 분리하여 그 중에 구연산 생성균 1종을 선정하여, 동정한 결과 Aspergillus niger로 나타났다. 분리된 균주로 500ml 삼각 flask에 Sakaguchi 배지 100ml를 넣고 3$0^{\circ}C$에서 발효한 결과 구연산이 17g/ι가 생성되었다. 또한 Sakagnchi 배지를 개량한 결과 즉 배지적응, peptone첨가, 통기 효과, methanol첨가 및 포도당 첨가 결과 구연산이 35g/ι가 생성되었다

• Journal of Microbiology and Biotechnology
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• 제3권3호
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• pp.209-213
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• 1993

The flow behavior of the mycelial broth of glucoamylase hyperproducer Asp. niger No. PFST-38 for the production of glucoamylase were studied. The mycelial broth followed Bingham-pseudoplastic flow model described by Herschel-Bulkley equation. The yield stress increased with the increase in mycelial concentration. The dependency of the consistency index and the flow behavior index on the mycelial concentration could be expressed by a linear relationship. The consistency index increased proportionally with the mycelial concentration while the flow behavior index decreased with the increase in mycelial concentration. The flow property of the broth was related to the morphological data obtain in the previous study. The changes in apparent viscosity of the broth could be expressed as a function of the hyphal thickness as shown below.

• 한국식품과학회지
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• 제28권2호
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• pp.366-370
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• 1996

흥삼분말의 위생적 살균을 위해 감마선 조사기법을 이용하였다. 감마선조사에 따른 오염 미생물의 살균효과는 호기성 전세균은 7.5 kGy, 곰팡이 및 대장균군은 2.5 kGy의 조사선량으로 검출한계 이하로 제거시킬 수 있었고 실온에서 6개월 저장후에도 미생물의 생육과 증식은 없었다. 홍삼분말에서 분리, 동정된 곰팡이는 Penicillium commune, Aspergillus niger, Aspergillus versicolor, Aspergillus unguis로 이들 포자의 $D_{10}$값은 각각 0.37-0.5 kGy. 0.24-0.31 kGy, 0.25-0.36 kGy, 0.28-0.41 kGy였으며, 2.5 kGy 선량에서의 불활성화계수는 각각 5-6.5, 7.4-9.3, 6.5·.9.1, 6.1-8.4이었다. 한편 홍삼 extract를 첨가한 배지가 PDA 배지보다 더 낮은 방사선 감수성을 보였고, 분리곰팡이의 배지특성에 따른 생육양상은 배양중기 이후부터 흥삼 extract 배지상에서 생육이 왕성하였다.

• Applied Biological Chemistry
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• 제19권3호
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• pp.139-144
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• 1976

전보(前報)에서 검토한 효소생산 최적조건에서 생성하는 cellulase의 특성(特性)에 대한 실험(實驗)을 행(行)하여 다음의 결과(結果)를 얻었다. 1. 조정제시(粗精製時)에 $(NH_4)_2SO_4$가 0.6포화도일 때 그리고 ethanol 농도(濃度)가 $80{\sim}90%$일 때 회수성(回收性)이 가장 좋았다. 2. 두 균주가 생산한 cellulase의 효소작용 최적 조건은 다음과 같다. Asp. niger-SM6 T. viride-SM10 최적(最適) pH 3.5 4.0 pH 안정성(安定性) $3.0{\sim}6.0$ $3.0{\sim}6.0$ 최적온도(最適溫度) $60^{\circ}C$ $60^{\circ}C$ 열안정성(熱安定性) $60^{\circ}C$이하(以下) $50^{\circ}C$이하(以下) 최적작용시간(最適作用時間) 30분(分) 60분(分) 최적기질농도(最適基質濃度) 3% 3% 3. CMCase에 대한 Km치(値)는 Aspergillus niger-SM6이 0.8%, Trichoderma viride-SM10이 1.01%였다. 4. Aspergillus niger-SM6은 xylanase 및 pectinase도 높은 활성(活性)을 가지고 있었다.