• 제목/요약/키워드: Ascospore

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한국산(韓國産) 자낭균류(子囊菌類) 버섯의 미기록종(未記錄種) (Notes on Unrecorded Fleshy Ascomycetes in Korea)

  • 홍순우;장용석
    • 한국균학회지
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    • 제15권2호
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    • pp.76-79
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    • 1987
  • 자낭균류에 속하는 100여 개체의 버섯표본을 관찰한 결과 3속 9종의 미기록 속과 종이 동정되었기에 보고하는 바이다. 미기록 속은 땅주발버섯 속(Tarzetta,), 살갗버섯 속(Mollisia), 점버섯속(Hypocrea) 등이다. 미기록 종은 노란긴대주발버섯(Cyathipodia cupuliformis), 솜털긴대주발버섯(Cyathipodia villosa), 갈색땅주발버섯(Tarzetta catinus), 오목땅주발버섯(Tarzetta cupularis), 갈색투구버섯(Cudonia confusa), 작은잎맥섯(Rutsroemia petiolorum), 노란상수리술잔고무버섯(Hymenoscyphus pileatus), 연한살갗버섯(Mollisia ventosa), 점버섯(Hypocrea rufa) 등이다.

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감나무 둥근무늬낙엽병균 Mycosphaerella nawae 분생포지의 2창 전염원으로서 역할 (Further Evidence that Ramularia-type Conidia in vivo Plays a Role as a Secondary Inoculum of Mycosphaerella nawae)

  • 권진혁;강수웅;박창석;김희규
    • 한국식물병리학회지
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    • 제14권5호
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    • pp.393-396
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    • 1998
  • The characteristic Ramularia type conidia of Mycosphaerella nawae were formed on naturally infected leaves. Artificial inoculation with the conidia induced typical symptom on leaves, which was not distinguishable from those of ascospore infection, which has been considered as a primary inoculum source in nature. Also the morphology of the conidia produced on PDA was not different from those formed on artificially inoculated leaves or on naturally infected leaves at later stage of symptom development. Accordingly, we report the role of the conidia as a secondary inoculum of the circular leaf spot pathogen of persimmon for the first time.

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Occurrence of Powdery Mildew on Safflower Caused by Sphaerotheca fuliginea in Korea

  • Kwon, Jin-Hyeuk;Kang, Soo-Woong;Lee, Heung-Su;Park, Chang-Seuk
    • Mycobiology
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    • 제28권1호
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    • pp.51-53
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    • 2000
  • The powdery mildew of safflower (Carthamus tinctorius L.) extensively occurred at 1999 at the experimental farm of Kyongsangnam-do Agricultural Research and Extension Services. Both sides of the leaves and the older stems were covered with the fungus, and then the leaves and stems turned yellow. The conidia, conidiophores and perithecia were observed on the leaf lesion. Perithecia were ellipsoidal, $80-117\;{\mu}m$ in diameter. Asci were subglobose and $84{\sim}99{\times}59{\sim}73\;{\mu}m$ in size. Ascospore were ellipsoidal to ovoid, and $15{\sim}34{\times}11{\sim}23\;{\mu}m$ in size. Conidia were ellipsoid to barrel-shaped, $25{\sim}37{\times}11{\sim}22\;{\mu}m$ in size and formed in long chains. The causal organism was identified as Sphaerotheca fuliginea. This is the first report on powdery mildew of safflower caused by Sphaerotheca fuliginea in Korea.

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Cultural Characteristics and Fruiting Body Production in Cordyceps bassiana

  • Lee, Je-O;Shrestha, Bhushan;Sung, Gi-Ho;Han, Sang-Kuk;Kim, Tae-Wong;Sung, Jae-Mo
    • Mycobiology
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    • 제38권2호
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    • pp.118-121
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    • 2010
  • Single ascospore isolates of Cordyceps bassiana were observed for their colony pigmentation on Sabouraud Dextrose agar plus Yeast Extract (SDAY) plates and were inoculated in a brown rice medium for production of fruiting bodies. Colony pigmentation did not show any relationship with perithecial stromata formation. The isolates were also grown on opposite sides of SDAY agar plates and were observed for vegetative compatibility. Neither vegetative compatibility nor perithecial stromata could be found to be related to each other. It was concluded that fertile fruiting body production was independent of colony pigmentation and vegetative compatibility. Synnemata formation was found to be more common than perithecial stromata formation. This might be due to its highly conidiogenous anamorphic stage, i.e., Beauveria bassiana.

Revisiting Rhytisma lonicericola: Morphological Characterization and Molecular Phylogenetic Analysis

  • Jung, Bok-Nam;Park, Ji-Hyun;Shin, Hyeon-Dong
    • Mycobiology
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    • 제50권2호
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    • pp.150-154
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    • 2022
  • Rhytisma lonicericola was identified as a tar spot fungus on Lonicera sp. in 1902, and has since been recorded on several species of Lonicera in China, Japan, and Korea. Most of the previous records of R. lonicericola have been based on a list of disease occurrences in the absence of any formal morphological identification or molecular analyses. Using six newly obtained specimens collected in the past 2 years, we confirmed the tar spot fungus found on L. japonica in Korea as R. lonicericola based on morphological examinations and molecular phylogenetic analyses. This fungus was distinguished from R. xylostei, another tar spot fungus on Lonicera, by ascospore size and geographical distributions. We present detailed mycological information and, for the first time, DNA sequence data useful for the identification of R. lonicericola.

목초액, 식물추출물 및 살균제를 이용한 표고골목해균인 주홍꼬리버섯 방제 (Control of Diatrype stigma Occurred on the Bed-log of Shiitake Using Wood Vinegar, Plant Extracts and Fungicides)

  • 박원철;이봉훈;가강현;조태수;이학주;이성숙;김명길;차병진
    • 한국균학회지
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    • 제34권1호
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    • pp.22-28
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    • 2006
  • 목초액, 잣나무추출물, 후추열매추출물 및 살균제를 이용해서 표고 골목에 발생하는 주홍꼬리버섯을 방제하고자 하였다. 식물추출물 선발시험결과, 목초액 35,000 ppm에서 균사생장이 완전히 억제되었고 25,000 ppm에서 자낭포자의 발아가 관찰되지 않았다. 그리고 잣나무추출물 200 ppm과 후추열매추출물 1,000 ppm에서 각각 98.9%와 95.9%의 자낭포자발아억제율을 보였다. 살균제 선발시험 결과, benomyl, carbendazim, thiabendazole 등의 최소억제농도(MIC)는 $0{\sim}0.4\;{\mu}g\;a.i./m{\ell}$였다. Difenoconazole은 $0.08%\;{\mu}g/m{\ell}$ 농도에서 자낭포자 발아를 98.9% 억제했다. 살균제에 의한 표고균사생장억제율 조사 결과, 저온성, 중온성, 고온성균주 간에 차이가 거의 없는 것으로 나타났다. Benomyl, carbendazim, thiabendazole, thiophanate-methyl 등은 높은 농도에서도 비교적 낮은 억제율을 보였으나, tebuconazole은 $0.4\;{\mu}g\;a.i./m{\ell}$ 농도에서도 80% 이상의 억제율을 보여 표고균에 좋지 않은 영향을 미치는 것으로 나타났다. 목초액 및 식물추출물과 살균제를 골목에 살포한 결과 목초액 150,000 ppm이 주흥꼬리버섯의 포자퇴형성 전 처리에서 72.7%의 방제가를 보였고 포자퇴형성 후 처리에서 목초액 70,000 ppm과 thiophanate-methyl 1,000 ppm이 각각 58.1%와 52.3%의 방제가를 보였다.

감나무 둥근무늬낙엽병의 발생과 병원균(Mycosphaerella nawae)의 전염원 동태 (Ecology of Disease Outbreak of Circular Leaf Spot of Persimmon and Inoculum Dynamics of Mycosphaerella nawae)

  • 권진혁;박창석
    • 식물병연구
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    • 제10권4호
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    • pp.209-216
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    • 2004
  • The circular leaf spot of persimmon is occurred almost every place where persimmon is cultivated, especially the disease outbreak severely in southern part of Korea. The disease reveals unusually long incubation period after pathogen invade into leaf tissue and no practical control measure is available once the symptom has appeared. Most of the farmers just follow the suggested spray schedules calculated on the basis of weather condition of ordinary years. Therefore the damages due to circular leaf spot greatly differ year after year. In this article, we tried to describe and summarized the investigation on the circular leaf spot pathogen, Mycosphaerella nawae, related to disease outbreak such as overwintering of pathogen, inoculum formation and spread, incubation period after infection, and secondary inoculum. With the summary of these results, we suggest the disease cycle of circular leaf spot of persimmon. The pathogen overwinters in diseased leaves as mycelial form or pseudoperithecial premodium. The pseudoperitheria become matured in spring as the temperature raise and forms asci and ascospores. The maturation of pseudoperithecia are closely related to the temperatures during March and early April. The ascospores completely mature in early May and the ascospores released when the pseudoperithecia absorbed enough moisture after rainfall. The release of ascospores are diverse greatly with the variation of maturity of pseudoperithecia. Generally the spore start to release from middle of May to early of July. Duration of ascospore release is depend on the weather condition of particular year, especially amount and number of precipitation. The ascospores produced from pseudoperithecia is known to the only inoculum for circular leaf spot disease. But according to the results obtained from our investigations, the conidia formed on the lesions which incited by natural infection. This conidia are infectious to persimmon leaves and formed identical symptom as natural infection. The time of producing secondary inoculum of circular leaf spot of persimmon is considered too late to develop new disease. Generally the importance of secondary inoculum is low but the conidia produced in early September are competent to develop new disease and new infection also significantly affect to harvest of persimmon. The importance of circular leaf spot disease is recognized well to farmers. The approaches to control of the disease should be initiated on the basis of the knowledges of inoculum dynamics and ecology of disease development. The forecasting system for circular leaf spot is need to be developed.

콩나물버섯과 미기록종 Mitrula paludosa (습지등불버섯 : 신칭)의 보고 (Note on the New Record of Mitrula paludosa (Geoglossaceae) in Korea)

  • 박용우;구창덕;홍대의;김태헌
    • 한국균학회지
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    • 제37권1호
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    • pp.104-107
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    • 2009
  • 본 연구에서는 국내 미기록종인 자낭균 콩나물버섯과 (Geoglossaccae)의 Mitrula paludosa Fr.에 대하여 속명(모자버섯속)과 종명(습지등불버섯)의 한국명을 부여하고, 이 버섯의 내외부 형태, 서식지, 발생시기 등 생태적 특징을 보고 하는 것이다. 습지등불버섯은 속리산국립공원의 얕은 계류의 소나무 낙엽에서 5월${\sim}$6월에 발생하였다. 이 버섯은 자실체 크기가 20-50 mm으로, 갓은 밝은 노랑${\sim}$주황색이고 주름이 진 원통형 내지 구형이다. 갓의 길이는 $10\;{\times}10{\sim}20 $ mm정도며 매끄럽다. 이와는 대조적으로 버섯의 대는 흰색으로 $2{\sim}3\;{\times}\;5{\sim}10$ mm 원통모양으로 갓과는 뚜렷하게 구분된다. 자낭은 무색이며 단막이고, 크기는 $90{\sim}110\;{\times}\;5{\sim}6{\mu}m$ 였다. 자낭의 측면에 배열하는 측사는 $95{\sim}120\;{\times}\;1.0-1.5{\mu}m$으로 무색의 바늘형이고, 4-5개의 격벽을 지니고 있고, 자낭보다 $5{\sim}10{\mu}m$ 정도 길었다. 자낭 안에는 무색인 8개의 포자가 머릿구멍이 없는 자낭 끝을 향하여 비스듬히 기울여져 있었다. 자낭포자는 크기가 $13-17{\mu}m\;{\times}\;2.5-3{\mu}m$로 길고 좁은 오이모양의 타원형${\sim}$방추형이었다.

Aspergillus nidulans에 있어서 무성분화(無成分化)의 억제조건(抑制條件)과 이를 이용(利用)한 유성분화결손(有性分化缺損) 돌연변이주(突然變異株)의 대량분리(大量分離) (Inhibitory Conditions of Asexual Development and their Application for the Screening of Mutants Defective in Sexual Development)

  • 한동민;한유정;이영훈;장광엽;장승환;채건상
    • 한국균학회지
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    • 제18권4호
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    • pp.225-232
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    • 1990
  • 몇몇 배양조건이 유성 또는 무성분화에 미치는 영향을 조사하여, 유성분화 결손 돌연변이주를 분리하기 위한 효율적인 조건을 찾고자 하였다. 무성분화는 억제하며, 유성분화는 촉진시키는 여러조건중 Casein hydrolysate를 첨가한 최소배지에 밀봉 배양하는 것이 가장 효율적인 조건으로 선택되었다. 접종 후 20시간 이전에 밀봉하고 계속 배양하면, 유 무성분화 모두가 억제되나, 밀봉 후 20시간 정도 후에 밀봉을 해제하면 유성분화만이 진행되었다. 이러한 성질을 이용하여 유성분화과정에 이상이 일어난 돌연변이주를 대랑 분리해 냈었으며, 이들의 분화양상에 따라 크게 3 group : NSD(never in sexual development), BSD(block in sexual development), ASD(abnormal in sexual development)으로 분류하였다. NSD 돌연변이주는 $H{\ddot{u}}lle$ cell이나 cleistothecia 등의 유성생식기관을 전혀 생성하지 못하였고, BSD 돌연변이주는 $H{\ddot{u}}lle$ cell, cleistothecia, crozier, asci 또는 ascospore 형성 중 어느 과정에서 중단되어 분화가 끝가지 진행되지 못하였으며, ASD 돌연변이주는 유성분화의 최종단계가지 진행은 되지만 유성생식 기관의 생성시기나 양이 야생형과 현저하게 차이를 보였다.

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수도갈색엽고병(Fusarium nivale)의 동정 (Identification of Fusarium leaf spot(Fusarium nivale) newly reported In Korea)

  • 권신한;송희섭;손청열
    • 한국응용곤충학회지
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    • 제12권3호
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    • pp.121-124
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    • 1973
  • 새로운 수도품종통일에 발생하는 갈색엽고병 유사증상을 발견하여 그 병반에서 병원균을 분리하여 그 형태를 조사하였으며 분생포자, 병반상에 형성하는 자남, 자남포자의 기재를 타연구자를의 문헌과 비교한 결과, 이는 일본에서도 보고된 바 있는 갈색엽고병임이 확인되었으며 병원균이 Fusarium nivale로 동정되었다. 또한 포장조건하에서는 본병은 다질소구에서 그 발생이 현저히 많았음을 관찰하였고, 본균의 생육적온은 $24^{\circ}C$였다.

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