The Korean Journal of Mycology (한국균학회지)
- Volume 18 Issue 4
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- Pages.225-232
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- 1990
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- 0253-651X(pISSN)
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- 2383-5249(eISSN)
Inhibitory Conditions of Asexual Development and their Application for the Screening of Mutants Defective in Sexual Development
Aspergillus nidulans에 있어서 무성분화(無成分化)의 억제조건(抑制條件)과 이를 이용(利用)한 유성분화결손(有性分化缺損) 돌연변이주(突然變異株)의 대량분리(大量分離)
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Han, Dong-Min
(Department of Molecular Biology, Wonkwang University) ;
- Han, Yoo-Jeoung (Department of Molecular Biology, Wonkwang University) ;
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Lee, Young-Hoon
(Department of Chemistry, KAIST) ;
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Jahng, Kwang-Yeop
(Department of Biology, Chonbuk National University) ;
- Jahng, Seung-Hwan (Department of Biology, Chonbuk National University) ;
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Chae, Keon-Sang
(Department of Molecular Biology, Chonbuk National University)
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한동민
(원광대학교 자연대 분자생물학과) ;
- 한유정 (원광대학교 자연대 분자생물학과) ;
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이영훈
(한국과학기술원 화학과) ;
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장광엽
(전북대 자연대 생물학과) ;
- 장승환 (전북대 자연대 생물학과) ;
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채건상
(전북대 자연대 분자생물학과)
- Published : 1990.12.30
Abstract
In order to find an useful condition under which the mutants defective in sexual development could be isolated, the effects of several cultural conditions on the developments of Aspergillus nidulans were examined. Among several conditions found to restrict the asexual sporulation but enhance the sexual process, the interference of aeration by sealing the plates with sealing film was the most useful one for the purpose of mutant isolation. Sealing at any time before 20 hours from inoculation prevented both sexual and asexual process. When the seal was removed after 24 hours, however, the mycelia developed only to sexual organs. Using this properity, the early morphogenic process of sexual development could be easily observed and a number of mutants that showed some defects in the process could be isolated. The mutants were divided into 3 groups, NSD (never in sexual development), BSD (block in sexual development) and ASD (abnormal in sexual development). NSD mutants never produced either the
몇몇 배양조건이 유성 또는 무성분화에 미치는 영향을 조사하여, 유성분화 결손 돌연변이주를 분리하기 위한 효율적인 조건을 찾고자 하였다. 무성분화는 억제하며, 유성분화는 촉진시키는 여러조건중 Casein hydrolysate를 첨가한 최소배지에 밀봉 배양하는 것이 가장 효율적인 조건으로 선택되었다. 접종 후 20시간 이전에 밀봉하고 계속 배양하면, 유 무성분화 모두가 억제되나, 밀봉 후 20시간 정도 후에 밀봉을 해제하면 유성분화만이 진행되었다. 이러한 성질을 이용하여 유성분화과정에 이상이 일어난 돌연변이주를 대랑 분리해 냈었으며, 이들의 분화양상에 따라 크게 3 group : NSD(never in sexual development), BSD(block in sexual development), ASD(abnormal in sexual development)으로 분류하였다. NSD 돌연변이주는